Objective To explore the molecular and cytogenetic abnormalities in multiple myeloma (MM).Methods The la-boratory data of bone marrow smears were retrospectively analyzed in 61 patients of MM.24 hours short-term culture of bone marrow and R banding technology were performed in 31 patients.Among these patients,10 cases were selected for de-tecting the IgH gene expression by the interval FISH method.Results The proportions of myeloma cells were 0.19~0.94 in bone marrow smears of 61 patients.In 31 patients,25 patients had enough metaphases for analysis,in which 19 cases (71.3%)had abnormal clones,8q24,11q13,13q14 and 17p13 were important structural abnormalities,where 14q32 rear-rangement was the most characteristic abnormal structure,6 patients were detected IgH gene rearrangement.Conclusion Bone marrow smear combined with other laboratory examinations could make the diagnosis of MM,chromosomal abnormali-ties may help to explore the pathogenesis of MM,and provide a theoretical basis for the early diagnosis,treatment and prog-nosis of this disease.

Objective To explore the reason of serious interference red blood cell(RBC)parameters in blood routine test and correct method.Methods 30 cases of patients whose results of blood routine RBC parameters obviously abnormal ,According to the interference factors were divided into four groups:cold agglutination (7 cases),severe hemolysis(13 cases),high triglycerides (8 cases),high white blood cell(WBC)(2 例).Take appropriate corrective action for processing respectively.The difference of red blood cell parameters were compared with t test.Results To analysis of interference factors and take appropriate corrective meas-ures,RBC parameters could be more close to the real results.Conclusion We should pay attention to the changes of the RBC pa-rameters,analysis of interference factors,and to take appropriate measures to correct,in order to avoid clinical misdiagnosis and mi-stherapy.

Objective:To investigate the correlation and predictive value of serum uric acid (SUA) and short-term clinical outcomes after intravenous thrombolysis in patients with acute ischemic stroke (AIS).Methods:Patients with AIS received intravenous thrombolysis in the First Affiliated Hospital of Kangda College of Nanjing Medical University from July 1, 2018 to March 31, 2022 were retrospectively enrolled. Fasting SUA, blood glucose and blood lipids were measured the next morning after admission. The modified Rankin Scale was used to evaluate the functional outcome at discharge. 0-2 points were defined as good outcome, and 3-6 points were defined as poor outcome. Multivariate logistic regression analysis was used to determine the independent risk factors for poor short-term outcome in patients with AIS after intravenous thrombolysis. The receiver operating characteristic (ROC) curve was used to evaluate the predictive value of SUA for poor short-term outcome after intravenous thrombolysis. Results:A total of 291 patients were enrolled during the study. Among them, 197 (67.70%) were male, aged 65.02±11.56 years. The median baseline National Institutes of Health Stroke Scale (NIHSS) score was 5 (interquartile range 3-11), and the SUA was 322.06±90.54 μmol/L. Univariate analysis showed that the age, proportions of patients with atrial fibrillation and cardiogenic embolism, baseline fasting blood glucose and NIHSS scores in the poor outcome group were significantly higher than those in the good outcome group, while the SUA after intravenous thrombolysis was significantly lower than that in the good outcome group (all P<0.05). Multivariable logistic regression analysis showed that higher SUA was independently associated with the good outcomes (odds ratio [ OR] 0.986, 95% confidence interval [ CI] 0.985-0.991; P<0.01), while older age ( OR 1.047, 95% CI 1.021-1.075; P<0.01) and baseline NIHSS score ( OR 1.155, 95% CI 1.063-1.254; P<0.01) were independently associated with the poor outcomes. ROC curve analysis showed that the area under the curve of poor outcome predicted by SUA was 0.642 (95% CI 0.552-0.732; P=0.002), the best cutoff value was 307.45 μmol/L, and the sensitivity and specificity of prediction were 57.7% and 68.0% respectively. Conclusion:Higher SUA is associated with the short-term outcome of patients with AIS after intravenous thrombolytic therapy, but its predictive value of the outcomes is limited.

Objective:To investigate the regulation of Parkin-dependent mitophagy mediated by calcyclin-binding protein and Siah-1 interacting protein (CacyBP/SIP) on apoptosis and cycle of dopaminergic (DA) neurons.Methods:SH-SY5Y cells were divided into model group, control group and CacyBP/SIP group; cells in the model group were treated with 1-methyl-4-phenylpyridine (MPP +, 0.5 mmol/L) for 24 h, and cells in the control group and CacyBP/SIP group were transfected with empty lentivirus or CacyBP/SIP-sgRNA lentivirus on the basis of MMP +(0.5 mmol/L) treatment for 24 h, respectively. Western blotting was used to detect the protein expression levels of CacyBP/SIP, microtubule-associated protein l light chain 3 (LC3), lysosome-associated membrane protein 2 (LAMP2), phosphatase and tensin homolog ten induced kinase 1 (Pink1), Parkin, P53, Bcl-2, and Bax; flow cytometry was used to detect the cell apoptosis and cycle; immunofluorescent single staining was used to detect the expressions of LC3 and LAMP2; immunofluorescent double staining was used to detect the coexpressions of CacyBP/SIP and Parkin. Results:As compared with the model group and control group, the CacyBP/SIP group had significant reduction in protein expressions of CacyBP/SIP, LAMP2, Pink1, and Parkin, LC3-II/I ratio, immunofluorescent staining intensities of LC3-II and LAMP2, and Bcl-2 protein expressions ( P<0.05). As compared with the model group and control group, the CacyBP/SIP group had significantly increased Bax protein expression, significantly decreased Bcl-2/Bax ratio, significantly increased apoptosis rate, significantly increased P53 protein expression, significantly increased proportion of cells at G1 phase, and significantly decreased immunofluorescent intensity of CacyBP/SIP and Parkin co-expressions ( P<0.05). Conclusion:After knocking out CacyBP/SIP gene, the decrease of Parkin protein leads to cell cycle being arrested at G1 stage, and mediates the decrease of Parkin-dependent mitochondrial autophagy, thereby leading to increased cell apoptosis.

Humans ; COVID-19 ; SARS-CoV-2