Objective To study the influence of small interfering RNA(siRNA) inhibiting Jumonji-containing JMJD3 gene on growth,proliferation and invasion of hepatocellular carcinoma cells.Methods JMJD3 was silenced by RNA interference(siR-JMJD3),non-specificity sequence pSilercer 2.1 was transfected into liver cancer cells QGY 7703 and HepG2 cells as the negative control.MTT assay,colony formation and Transwell invasion assays were used to detect the cell growth,proliferation and invasion ability of hepatocellular carcinoma cells.Results The Western Blot detection results showed that the siR-JMJD3 plasmid transfected experiment group successfully inhibited the JMJD3 protein expression level in liver cancer cells.The MTT results showed that after transfecting siR-JMJD3,the growth activity of QGY-7703 and HepG2 were decreased by 25% or 17% compared with the control group,.The colony formation results showed that in the colony formation number of two cell lines were decreased by 31% and 25% respectively.The Transwell invasion test results indicated that transmembrane cells number was decreased by 44% and 47% respectively.Conclusion Using siRNA technique can effectively inhibit JMJD3 gene expression,meanwhile effectively inhibits the in vitro growth,proliferation and invasion of liver cancer cells QGY-7703 and HepG2 and provides a new thinking for the biological therapy of liver cancer.

OBJECTIVE:To investigate the general regularity and characteristics of ADR induced by quinolones,and to pro-vide reference for rational drug use in the clinic. METHODS:A total of 84 quinolones-induced ADR reports in our hospital from 2011 to 2014 were analyzed statistically in respects of general information,drug use,organs/systems involved in ADR,clinical manifestations,outcome and DDDs. RESULTS:The adverse reactions were caused by 7 kinds of drugs,among which 89.29% of them were caused by intravenous injections,manifesting as lesion of skin and its appendants,digestive system and nervous system injury,etc. The proportion of female patients(60.71%)was higher than that of male(39.29%). No serious ADR was found,and all patients were recovered or cured. CONCLUSIONS:Gatifloxacin injection shows the low safety. The monitoring of ADR in-duced by quinolones should be strengthened to ensure the safe and effective use of drugs.

OBJECTIVE:To provide reference for rational use of antibiotics during perioperative period of transcatheter arterial chemoembolization(TACE). METHODS:After full evaluation of 1 cases of primary liver cancerpatient’s conditions,the optimal clinical evidences were retrieved and evaluated,which were related to antibiotics regimen during perioperative period of TACE. Us-ing“hepatocellular carcinoma”“antibiotic prophylaxis”“hepatic cancer”“TACE”as subjects,the evidence were retrieved from Co-chrane Library(issue 4,2015),Medline(1980 to 2015)and CJFD(1990 to 2015)and then evaluated. RESULTS:1 meta-analy-sis or systematic review,10 RCTs and 2 practice guidelines were identified. This patient didn’t need to use antibiotics to prevent in-fection according to literature analysis. CONCLUSIONS:The rational treatment plan according to evidence-based medicine methods for patients can not only promote the rational use of antibiotics,reduce bacterial drug resistence and treatment cost,but also guide both doctors and patients to take the indeterminate risk of medicine.

Human parainfluenza virus (HPIV)is one of the most common pathogens of community acquired respiratory infection.The epidemiology and clinical features vary in different subtypes.HPIV also affect other systems except respiratory system.The sequences of nucleotide and amino acid showed high identity among the strains in the same type,while remaining some variation.Most research shows the variation associated with time and district.Hemagglutinin-neuraminidase(HN) protein shows most polymorphic among all the proteins of HPIV.Some amino acid variation among the active site of HN protein will affect its activity.The influence of many gene mutations,which lead to amino acid variation,and affect the activity of HN protein remains uncertain.Further study on the epidemiology,clinical features,phylogenetics analysis of HPIV plays an important role in the prevention and control of its prevalence.

Drug resistance is one of the major obstacles for chemotherapy and targeted therapy in lung cancer with complex mechanisms.The researches have demonstrated that epigenetic changes are closely related to drug resistance of tumor.DNA methylation is an important epigenetic modification.In this paper, the relationships between hMLH1 promoter aberrant methylation and platinum-resistance, and DAPK promoter aberrant methylation and EGFR-TKI resistance in lung cancer will be briefly reviewed.

Host cell proteins(HCPs) of biological products are endogenous host proteins expressed by host cells or produced when they die during the production of biological products. Incomplete elimination of HCPs is the main factor causing allergy or other adverse reactions. The analytical methods for HCPs mainly include SDS-PAGE, Western blot(WB), two-dimensional gel electrophoresis(2DE), ELISA, liquid chromatography, mass spectrometry and so on. Electrophoresis can provide qualitative and semi-quantitative determination for proteins, but the resolution and accuracy are low. ELISA has good reproducibility and high sensitivity, and is most widely used at present. Chromatography and mass spectrometry have high separation and resolution as well as accurate quantification, but high application cost. ELISA was used to determine HCPs in recombinant biological products in the general rules <3412>, <3413> and <3414> of Chinese Pharmacopoeia(Volume Ⅲ, 2020 edition)and United States Pharmacopoeia(USP) <1132>. Analysis of HCPs components is helpful to improve the coverage of antiHCPs antibody and the sensitivity and specificity of detection methods. Traditional analytical methods can only qualitatively analyze HCPs, but cannot perform quantitative determination and characterization. Based on electrophoresis technology and mass spectrometry technology, combined with the development trend of HCPs component analysis at home and abroad, this paper summarizes the relevant HCPs analysis method to provide a reference for the verification of HCPs in the research and production process of biological products.

Objective: To establish a method for the quality standard of Biyankang granule. Methods: Scutellaria baicalensis Georgi and Puerariae Iobatae radix were identified by TLC. The content of baicalin was determined by HPLC with a Dubhe C18 column (250 mm × 4. 6 mm, 5 mm). The mobile phase was methanol and 0. 4% phosphoric acid with gradient elution. The flow rate was 1. 0 ml· min-1 and the detection wavelength was 280 nm. The column temperature was 30℃ and the injection volume was 10 ml. Re-sults:The spots in TLC were clear without any interference. A good linear relationship was shown within the range of 15. 02-300. 43 m g·ml-1 for baicalin (r=1.000 0). The average recovery of baicalin was 102.62% (RSD =0.72%, n =6). Conclusion: The method is specific with high accuracy and good reproducibility, which can be used for the quality control of Biyankang granule.

Objective To evaluate the curative effects of venlafaxine in patients with depressive disorder between therapeutic modes of inpatient and outpatient. Methods Seventy-two patients with depressive disorder including 36 inpatients and 36 outpatients were measured respectively with HAMD24 after treatment for 1 week, 2 weeks, 4 weeks and 8 weeks, and then the curative effects were compared between two groups. Results It was found that all patients in the inpatient group completed the observation and two patients in the outpatient group didn’t complete the observation. The HAMD24 score was significantly decreased in the inpatient group than that of the outpatient group from the second week to the end of the eighth week (P<0.05). The total effective rates were 80.6% for inpatient group and 64.7% for outpatient group, no significant difference between them. Conclusion Venlafaxine shows a higher therapeutic value and outcome in the therapeutic mode of inpatient.

Objective To study the expression levels of aerobic glycolytic enzymes in the cerebellums and its correlation with pathogenesis of autism in patients with autism. Methods The Western blotting was used to quantita-tively examine the expression levels of aerobic glycolytic enzymes, including HK-Ⅰ, HK-Ⅱ, PFKP, PKM1/2, PKM2, GAPDH, PDH and LDHA in the cerebellums of eight patients with autism and eight age-matched controls. Results Compared to controls, PDH expression was significantly decreased [(0.715±0.342) vs.(1.028±0.203), P=0.043], while expression of other seven aerobic glycolytic enzymes remained unchanged ( P>0 . 05 ) in the cerebellums of patients with autism. Conclusion The present study has revealed a decrease in the expression of PDH in the cerebellums of patients with autism, which may be involved in the pathologic process of autism.

The seven-year system in medical education is a new teaching model of cultivating the high-level medical talents to meet the need of construction of socialist health modernization of our country.As a student having had medical study for two years,I realize some advantages and shortcomings existing in the class education.So I have made analysis of the current conditions of its basic medical education and put forward some proposals,hoping to provide help for the seven-year teachings in the undergraduate courses.