Objective:To approach the determination method of 5-hydroxymethyl-furfural in Radix Rehmanniae,and to investigate influence of steam processing on catalpol and 5-hydroxymethyl-furfural. Methods:Catalpol and 5-hydroxymethyl-furfural from Radix Rehmanniae at different processing time was determined by high performance liquid chromatography. Results:In the steam processing,content of 5-hydroxymethyl-furfural increased with the increase of time in the beginning,when steamed to 52h,the levels began to decline; content of catalpol reduced with increase of the processing time. Conclusion:Content of catalpol and 5-hydroxymethyl-furfural changed signifi cantly in the steam processing.

Objective To evaluate the therapeutic effect of total mesorectal excision in radical operation of rectal carcinoma.Methods From 1997 to 2007,126 consecutive patients with middle and inferior rectal carcinoma underwent surgical operation according to the principle of total mesorectal excision.Results Two patients(1.6%) died of postoperative pulmonary infection,and postoperative anastomotic leakage occurred in 2 cases(1.6%).The postoperative follow-up of 1-5 years showed that local recurrence rate 2 years after surgical operation was 4%(5/126),the 3-,5-year survival rate was 93.6%(116/124) and 84.7%(105/124),respectively.Conclusions Total mesorectal excision can decrease the frequency of postoperative local recurrence of rectal cancer without increasing complications as long as its correct indications are selected.

Objective To build a method for content determination of Hypericin in Guanye Jinsitao drop pills by HPLC.Methods The column was Kromasil C18 (150 mm×4.6 mm, 5μm), mobile phase was acetonitrile-0.1% phosphoric acid (16∶84), UV detection was performed at 360 nm, the flow rate was 1.0 mL/min, and the detector drift tube temperature was set at 30℃.Results The calibration curves were linear in the range of 0.132-1.188μg for Hypericin (r=0.999 9). The average recovery was 99.55% (RSD=0.78%).Conclusion The method is simple, accurate, reliable, and can be used for the quality control of Hypericin in Guanye Jinsitao drop pills.