Objective To investigate clinical value of anti-adhesion agent:chitosan in preventing adhesions followed by laparotomy surgery in obstetrics and gynecology.MethodsFrom Jan.2006 to Dec.2009,770 patients underwent laparotomy surgery at Department of Obstetrics and Gynecology in the Second Affiliated Hospital of Harbin Medical University.One hundred and twenty-five patients underwent secondary surgery due to disease recurrence or cesarean section,the previous surgery were 18 cases with myomectomy,20 cases with endometriosis surgery,5 cases with resection of pelvic abscess,20 cases with ectopic pregnancy surgery,27 cases with benign adnexal neoplasm resection,9 cases with cyto-reductive surgery in ovarian cancer,26 cases with variable infertility surgery.Those 125 patients were managed by different agents washing before abdomen closure,which were assigned into two groups,including 59 cases washed by saline in control group and 66 cases washed by chitosan in study group.The abdomen adhesion in secondary surgery was evaluated by adhesion classification system.Results In study group,37 patients without adhesions,20 patients with degree Ⅰ adhesion,6 patients with degree Ⅱ adhesion,3 patients with degree Ⅲ adhesion,and no patients with degree Ⅳ adhesion were observed.While in control group,there were 11 patients without adhesions,23 patients with degree Ⅱ adhesion,14 patients with degree Ⅱ adhesion,8 patients with degree Ⅲ adhesion,and 3 patients with degree Ⅳ adhesion.The distribution of adhesion reached statistical significance between the two groups (x2 =20.9999,P =0.0003 ).Twenty-six patients in previous surgeries due to infertility included 17 cases in control group and 9 cases in study group.They all were managed by cesarean section in secondary surgery,it was found that 15 cases without adhesion and 2 cases with degree Ⅰ adhesion were in study group and 2 cases without adhesion and 7 cases with degree Ⅰ adhesion were in control group.It reached statistical difference ( P =0.0016 ).ConclusionAnti-adhesion agent could prevent adhesion followed by surgery in obstetrics and gynecology effectively.

OBJECTIVE To investigate and control the risk factors about hospital infection in patients with chronic severe hepatitis B. METHODS Totally 466 hospitalized patients with chronic severe hepatitis B in our hospital from Jan 2001 to Dec 2007 were studied retrospectively. RESULTS The total incidence of hospital infection was 20.1% for inpatients with chronic severe hepatitis B,the infection located as follows:56 patients were in lower respiratory tract (59.6%);17 patients in upper respiratory tract (18.1%);7 patients with bacteremia (7.4%);3 patients in urinary tract (3.2%);7 patients in gastrointestinal tract (7.4%);and 1 patients was in cutis tissue (1.1%). CONCLUSIONS The knowledge about the on infection risk factors liver disease must be strengthened by hospital medical staff of the use of antibiotics must be strict controlled in principle,operation must be aseptic,the concept of prevention standards must be enhanced,and the primary disease must be actively treated,all these can effectively reduce infection in hospital.

Objective To evaluate which b value is the best to distinguish the glioma recurrence and radiation-induced brain injury using DWI when b value were at 1 000 s/mm2、3 000 s/mm2 and 5 000 s/mm2 respectively.Methods Retrospective analysis the DWI of 21 patients who suffered from glioma recurrence and radiation-induced brain injury obtained on a 3T MRI scanner.Results (1)All recurrent glioma (100%)showed hyper-intense signal,while most radiation-induced brain injury patients (80%)showed hypo-in-tense signal when b=5 000 s/mm2 .The sensitivity and specificity were high (100% and 80% respectively)when hyper-intense sig-nal was taken as a diagnostic point of glioma recurrence and radiation-induced brain injury.(2)The receiver operating characteristic (ROC)curve analysis suggested that the minimum ADC resulted in a highest sensitivity to differentiate glioma recurrence from radi-ation-induced brain injury when b=5 000 s/mm.Conclusion High b-value is more accurate to reflect cell density,and the minimum ADC is better to differentiate the glioma recurrence and radiation-induced brain injury when b=5 000 s/mm2 on DWI.

Objective The purpose of this study was to explore the relationship between the loss of the gene deleted in colorectal carcinoma (DCC) gene expression in ovarian carcinoma and the transformation, progression of the tumor and its clinicopathological factors. Methods DCC gene mRNA expression were examined by reverse transcription polymerase chain reaction (RT-PCR) in 34 malignant, 10 benign and 10 normal ovarian samples. To clarify the expression of DCC gene by the DNA cloning and the DNA sequencing analysis in normal ovarian sample. Results The expression of DCC gene was lost in no normal ovarian tissues, in 2 (2/10) benign lesions, while the loss of DCC gene expression was found in 19(19/34,56%) carcinomas ( P

Objective To evaluate the inhibitory effect of endostatin on ovarian cancer cell line SKOV3 and to investigate the possible mechanism of the inhibition.Methods The effect of endostatin on SKOV3 cell proliferation was studied by means of MTT.The cell apoptosis was detected by transmission electronic microscopy.BCL-2 and BAX expressions were measured in SKOV3 cells treated by endostatin by immunocytochemistry,RT-PCR and Western blot.Results Endostatin inhibited SKOV3 cell proliferation(P

Objective To analyze the clinical and laboratory manifestations of primary Sj(o)gren's syndrom (pSS) with neurological involvement.Methods One hundred and forty eight patients fulfilling the 2002 American-European pSS classification criteria were retrospectively analyzed.Neurological manifestations were diagnosed based on the clinical,biological,electrophysiological,and imaging findings.Biographical,clinical,and laboratory data were compared between patients with and without neurological manifestations.Statistical methods used were Mann-Whitney U test,Chi-square test and Fisher exact probability.Results The prevalence of neurological involvement in pSS was 20.3％ (30/148),and the incidence of peripheral neuropathy,the central neuropathy and combination of the central neuropathy with peripheral neuropathy were 10.1％(15/148),9.5％(14/148) and 0.7％(1/148),respectively.The clinical spectrum of peripheral neuropathies encountered in Sj(o)gren's syndrome (SS) patients varied,with the pure sensory neuropathies being the most common,followed by sensorimotor neurophathies.Motor neuron disease was the most common type of central neurophathies.Compared with those without neurological manifestations,the duration of peripheral nerve system/central nerve system (PNS/CNS)-pSS patients was relatively short [(55±76) months vs (100±108) months,Z=-2.682,P＜0.05],and the antinuclear antibody (ANA) titer and RF titer were lower [(234±248) vs (377±339),Z=-2.008,P＜0.05;(126±279) U/ml vs (359±1 445) U/ml,Z=-2.243,P＜0.05].In PNS/CNS-pSS patients,the most common clinical manifestations included numbness (50％),pain (23％),and muscle weakness (63％).Conclusion The prevalence of neurological involvement in pSS is high.The duration is relatively short and the disease activity is high,but the disease features are atypical and may be neglected by rheumatologists.

Background and purpose:Ovarian cancer is one of three common malignant tumors in the female reproductive system,whose mortality is the highest in all of the gynecological cancers.The genetic instability and hypermutation rate of tumor cells were found to be associated with chemotherapeutic resistance,and accounted for the high recurrence rate and the failure of current treatment.Invasion and metastasis of tumor are closely related to angiogenesis.Experiments in vivo and in vitro have confirmed that there are many kinds of promoter and suppressor of angiogenesis in primary malignant tumor cells.Angiogenesis could be a potential molecular target for cancer treatment.Endostatin is a specific inhibitor of vessel endothelium.Animal experiments have confirmed that Endostatin could inhibit tumor growth through anti-angiogenesis and result in the decrease of invasion and metastasis of cancer,and it would not easily induced drug resistance in the cells.Thus Endostatin could be safely used for a variety of cancer treatments,being able treat any patho-angiogensis.Recently it has been reported that Endostatin may significantly enhance anti-tumor effects when it is combined with traditional treatment methods such as surgery,chemotherapy,radiotherapy and immunotherapy.Although the study of Endostatin was done in vitro,the primary results were very encouraging and provide the rationale for future clinical trials.In this study,we aim to evaluate the inhibitory effect of Endostatin on SKOV_(3) cell and to investigate the possible mechanism of the inhibition.Methods:1.The effects of Endostatin on SKOV_(3) cells proliferation was studied by means!of MTT.2.The cell apoptosis and cell cycle were detected by transmission electron microscope and cell flow cytometry.3.bcl-2 and bax expression were determined in SKOV_(3) cell by immunocytochemistry,RT-PCR and Western blot analysis.Results:Endostatin inhibited SKOV_(3) cell proliferation,and the value of A_(490) of 15?g/ml 72 h Endostation group(0.454) was much lower than that of PBS control group(1.369)(P

Objective To analyze the CT imaging features of Castleman disease and enhance our knowledge of Castleman disease.Methods Twenty two patients with lymph node biopsy-proved or surgeryproved Castleman disease were retrospectively reviewed in this study.Of the 22 patients,18 had localized lesion and 4 patients had multicentric lesions.Correlation was made between CT and pathologic findings.Results Eighteen patients with localized Castleman disease had the hyaline-vascular type and showed well-circumscribed masses with soft-tissue density [mean CT value,(45 ± 16) HU],punctate or bifurcate calcification and linear low-density areas on non-enhanced CT images.All localized masses showed significant enhancementwith an increase of(56 ± 22)HU on arterial phase and showed residual enhancement and some low-density areas on delayed phase.Enhancing patterns were variable,including homogeneous enhancement,gradual enhancement from the edge to the center of mass and heterogeneous enhancement.Four patients with localized lesion demonstrated enhancing vessels around masses.Four patients with muhicentric CD belonged to the plasma cell type and had multiple enlarged lymph nodes.Plasma cell type masses with homogeneous density also showed enhancement after injection of contrast media but appeared to reveal a less increase of (32 ± 10) HU than the hyaline vascular type.Conclusions The localized Castleman disease showed certain characteristics on CT imaging includingcalcification and contrast enhancing patterns,which could help in the differential diagnosis of this disease.The muhicentric Castleman disease did not reveal any useful imaging features.

Objective To observe the clinical efficacy of ozone injection combined with extracorporeal shock wave in treatment of arthritis of perishoulderr.Methods 120 patients with arthritis of perishoulderr and treated were choiced,they were randomly divided into two groups.Patients in group C were performanced extracorporeal shock wave treatment.Patients in group H were performanced with ozone injection combined with extracorporeal shock wave.Visu-al analogue score(VAS)and rane of motion(ROM)were used to evaluate the therapeutic effect.Results There was no significant difference between the two groups preoperation.Then significant difference of VAS in the two groups for 1week,1 month,3 months and 6 months after treatment showed respectively as (4.56 ±0.27)points vs.(2.49 ± 0.25)points,t =43.57,(3.96 ±0.23)points vs.(1.86 ±0.29)points,t =43.95,(3.36 ±0.20)points vs.(1.79 ± 0.25)points,t =36.51,(3.98 ±0.23)points vs.(2.09 ±0.27)points,t =41.29,P <0.05;The ROM data were respectively(55 ±18)points vs.(67 ±16)points,t =3.86,(60 ±20)points vs.(72 ±18)points,t =3.45,(56 ±19) points vs.(78 ±15),t =7.04,(46 ±18)points vs.(77 ±14)points,t =10.53,P <0.05.Patients feeled pain eased significantly in group H was significantly higher than group C in 6 month postoperativeiy.(χ2 =38.90,P <0.01;χ2 =8.91,P <0.05).Conclusion There is significant effect in patients with arthritis of perishoulderr treated by com-bined ozone injection with extracorporeal shock wave.

Twenty-seven in-patients with hemiplegia following brain injury were studied by using upper extremity median nerve somatosensory evoked patentials (SVEP), Brunnstrom assessment in hemiplegic hand and assessment of the patients' activities of daily lioing (ADL) (Barthel index). The upper extremity median nerve SEP on the affected and normal sides was determined. By using Kovindha standard, upper extremity median nerve SEP was graded in accordance with N20. The correlation between the differences of SEP N20 amplitude and the latencies on the both sides and the Barthel index scores was analyzed. A Spearman correlation analysis was made between the median nerve SEP N20 grades and Brunnstrom stages in hand or ADL on the affected side. The results showed that upper extremity median nerve SEP grades were positively correlated with those of the Brunnstrom stages in hand (r1 = 0.6925, P1 < 0.01). The correlation coefficient between SEP N20 grades and patients' ADL grades was r2 = 0.5015, P2 < 0.01. It was concluded that upper extremity median nerve SEP could be used as a sensitive electrophysiological predictor to clinically assess hemiplegic hand function. SEP N20 might play a role in predicting the ADL of the patients with hemiplegia to some extent, but could not be used as a sensitive predictor to directly observe and predict the ADL of the patients.
Adult ; Aged ; Brain Injuries ; complications ; physiopathology ; Cerebral Infarction ; complications ; physiopathology ; Evoked Potentials, Somatosensory ; Female ; Hemiplegia ; etiology ; physiopathology ; Humans ; Male ; Median Nerve ; physiopathology ; Middle Aged