1.Analysis on the Communication Barriers between Doctors and Patients
Journal of Medical Informatics 2009;30(7):54-56
Communication barriers between doctors and patients are the main reasons leading to contradictions between doctors and patients. The paper analyzes the existing barriers in the information communication process, including language barrier, time barrier and communication psychological barrier, some possible strategies of dredging are proposed such as popularizing the medical information, establishing communication mechanism between doctors and patients, and improve the communication ability of doctors.
2.Causes and prevention low rectal cancer after anterior resection of anastomotic leakage
Peifeng LI ; Yu WANG ; Lie WANG
International Journal of Surgery 2009;36(7):451-454
Anus fine of low rectal cancer surgery, can make the quality of Life of patients significantly im-proved,but postoperative anastomotic leakage is still the main complication, its occurrence can lead to in-creased perioperative mortality, prolonged hospitalization, increased cost, causing a great deal to patients suffering. In this paper, we review the causes and prevention of low rectal cancer after anterior resection of anastomotic leakage.
3.Application of social tagging in health social networks
Fangfang LI ; Yangfang TAI ; Peifeng HE
Chinese Journal of Medical Library and Information Science 2014;(11):23-28
Social tagging, as an important method for organizing network resources, has been widely applied in different kinds of website with the development of Web2.0 technologies.Proposed in this paper are the suggestions for promoting the better development of healthsocial networks, such as establishing social login model, developing tag library, constructing tag automatic classification system, implementing personal recommendation system, analy -zing tag semantemes , and limiting users login rights according to the application of social tagging in health social networks and related problems needing to be solved .
4.Treatment of Infantile Muscular Torticollis with Tuina: A Report of 22 Cases
Peifeng HAN ; Yanping LI ; Chouping HAN
Journal of Acupuncture and Tuina Science 2004;2(1):53-54
Twenty-two cases of infantile muscular torticollis were treated with such Tuina manipulations as pushing, kneading, grasping and wrenching with the following result: 18 cases got recovery, 3 cases got marked effect and 1 case got no effect. The whole effective rate was 95.4%.
5.Effect of tagging on library information ecosystem and its countermeasures
Fangfang LI ; Yangfang TAI ; Peifeng HE ; Xiaoxia LI ; Caiyu LIU
Chinese Journal of Medical Library and Information Science 2015;(4):1-4
Librarians play a key role in the library information ecosystem.Librarians and library users interact and influence each other in process and use of information.Tagging defined by network users is used in description of words and phrases in different information resources.When it is used in library, user-oriented service will become the target of information service, and the role of information service persons, information organization methods and information low efficiency will change.However, rational planning of ecosystem, rational control of tagging, and improvement of librarian information literacy can reduce the negative effect of tagging.
6.Regulation effect of taurochenodeoxycholic acid immunologic function in mice
Youfei SHI ; Peifeng LI ; Jinlian LI ; Hong GUAN
Chinese Pharmacological Bulletin 2003;0(07):-
Aim To study the effect of Taurochenodeoxycholic Acid (TCDCA) on immunologic cell in vitro and in vivo in mice. Methods Five doses were used , including 0.043, 0.065, 0.1, 0.153, 0.189 g?kg-1 in vivo and 0.01, 0.05, 0.1, 1, 10 mg?L-1 in vitro. Effects of TCDCA on peritoneal macrophage in mouse in vitro,splenic lymphocyte, normal organism, immunologic functional inhibiting model and nerve-endocrine-immunoregulation system were investigated. Results TCDCA had the obvious immunoregulation effect on normal organisms in mouse. 0.1 g?kg-1 TCDCA had the obvious anastated effects on the immunological inhibiting model made up by the cyclosporine A and cyclophosphamide. TCDCA may affect the peritoneal macrophage and splenic lymphocyte in vitro directly, meanwhile two kinds of immunological cells showed the obvious immunoregulation effects on the whole. 0.1 g?kg-1 TCDCA can increase the hydrocortisone contents of the nerve-endocrine-immunoregulation system in mouse most remarkably. Conclusion TCDCA had the adjustment effect on the immunoregulation function in mouse.
7.Antispasmodic Anti-inflammation and Analgesic Effect of Clematis
Yunyi ZHANG ; Hongwei ZHANG ; Peifeng LI ; Binglin CHENG ; Ji FU
Chinese Traditional Patent Medicine 1992;0(11):-
Objective: To evaluate the pharmacodynamic of clematis. Methods: 1) The relaxation of isolated ileum muscle of guinea pig by clematic was tested. The antagonism of clematis on histamine or Ach induced ileum muscle contraction was tested also. 2) By using the model of turning of trunk, the pain releasing effect of clematis was evaluated. 3) The experiments about anti-inflammation action of clematis were carried out in two models. Results: Clematis relaxed the ileum muscle. It also antagonized the contraction of the muscle caused by histamine and Ach. A single intrapeditional administration of clematis to Kun-Ming mice significantly prolonged the latency and reduced the writhe number at the turning of trunk model; clematis dose-dependently inhibited the mouse ear swelling caused by xylol. It also had anti-inflammation effect at the other model. The results demonstrated that clematis could release the pain, suppress the inflammation and smooth muscle contraction.
8.Simultaneous Determination of 3 Optical Isomers in Carfilzomib by HPLC
Haixia LI ; Na LIU ; Peifeng BAI ; Jingyuan LIU ; Wenmin GUO
China Pharmacist 2016;19(6):1094-1095,1101
Objective:To establish an HPLC method for the determination of optical isomers in carfilzomib .Methods:The sample was separated on a Chiralpak OX-H column.The mobile phase consisted of n-hexane∶isopropanol∶alcohol (89 ∶5 ∶6, v/v/v) with a flow rat of 1.0 ml· min-1 .The detection wavelength was 220 nm.Results:The linear rang of enantiomer , diastereomer Ⅰand di-astereomer Ⅱwas 0.54-2.14 μg· ml -1,0.11-1.80 μg· ml-1 and 0.11-1.81 μg· ml-1(r≥0.998), respectively.The lower limit of quantification was 0.07-0.27 μg· ml-1 .The recoveries of optical isomers were within the range of 99.6%-100.9% with RSD of 1.13%-1.59%(n=9).Conclusion:The method is sensitive, simple, fast, accurate and specific, and suitable for the study of opti-cal isomers in carfilzomib .
9.Preparation of the Coxsackievirus A16 VP1 subunit vaccine and detection of its immunogenicity
Xiaonan LI ; Deyan LUO ; Zhongpeng ZHAO ; Yueqiang DUAN ; Peifeng LI ; Xiliang WANG
Chinese Journal of Microbiology and Immunology 2010;30(3):250-255
Objective To prepare VP1 protein vaccine of Coxsackievirus A16(CA16) and evalu-ate immunngenicity the subunit vaccines of Coxsackievirus (VP1), and to establish foundation for studying CA16 vaccine. Methods CA16 VP1 was amplified by RT-PCR and cloned into pFastBac HT A plasmid, recombinated with Bacmid DNA by transposition reaction and then transfected Sf9 cell, mixed with adjuvant AI(OH)_3. After immunization BALB/c mice, evaluating immune effectiveness after booster injections 2 weeks. Results The expressed protein was analyzed by SDS-PAGE and Western blot, mice immunized with CA16 (VP1) both induced specific IgG antibody and neutralization antibody. The best immunization antigen was 20 μg, IgG antibody was 1: 1600, neutralization antibody was 1:250, typical Th1/Th2 immune response was determined by lymphocyte proliferation assay and cytokine analysis. Conclusion The CA16 VP1 gene was cloned successfully and expressed in Sf9 insect cells, CA16 VP1 protein vaccine induced both humoral and cellular immune response, to lay solid foundation for further study on CA16 vaccine.
10.Roles of GITR/GITRL signal system expression at bloodstream lymphocytes in children with asthma
Li FANG ; Rongrong LI ; Fangjun LUO ; Liyan ZHAO ; Peiying CHEN ; Peifeng YU
Chinese Journal of Primary Medicine and Pharmacy 2015;(23):3558-3560
Objective To investigate the expression of blood CD +4 CD +25 Treg GITR,CD +4 T cell GITRL in children with asthma,and the role of them in asthmatic inflammation.Methods 50 cases of severe asthma were selected,and were controlled with thirty two healthy children.The venous blood was collected both in the period of acute episode and clinic remission.The mean fluorescence intensity of CD +4 CD +25 Treg GITR and CD +4 T cell GITRL was detected by flow cytometry.Results The expression of CD +4 CD +25 Treg GITR in the asthma acute period group was (24.2 ±8.2)MFI,which was significantly lower than (28.5 ±6.0)MFI in the control group(t =2.5,P <0.05).While there was no significant difference of CD +4 T cell GITRL expression between the asthma acute period group[(5.2 ±3.6)MFI]and the control group[(4.6 ±1.0)MFI](t =1.1,P >0.05).Moreover,the expression of CD +4 CD +25 Treg GITR in the asthma in remission group after treatment was (29.5 ±8.3)MFI,which was significantly higher than that in acute period group before treatment(t =-9.9,P <0.01 ).Whereas,there was no significant difference of CD +4 T cell GITRL expression in asthma group between in remission after treatment[(5.7 ±3.6)MFI] and in acute period before treatment(t =-1.6,P >0.05).Furthermore,there was no significant correlation between levels of CD +4 CD +25 Treg GITR and CD +4 T cell GITRL.Conclusion The level of CD +4 CD +25 Treg GITR in acute period asthmatic patients was decreased,but it was increased in remission,but no changes of CD +4 T cell GITRL expression were observed.GITR/GITRL signal system might be involved in the asthmatic inflammation procession.