Objective:To investigate the expression of a new cytokine,thymic stromal lymphopoietin (TSLP) and its receptor TSLPR in the villi of human first trimester gestation.Methods:Villi were collected from women who had undergone an artificial abortion at 7-11 weeks of normal gestation.The trophoblast cells (Tros) were isolated and cultured.The total RNA was extracted using TRIzol reagent,from both villi and the Percoll-gradient-isolated Tros,then the DNA fragments of hTSLP and hTSLPR were amplificated by RT-PCR.Villous tissues were detected for TSLP by immunohistochemistry (IHC) and Western blot.Immunocytochemistry (ICC) was carried out on cultured trophoblast cells for TSLP/TSLPR expression.Levels of TSLP in the supernatants were detected by ELISA.Results:Normal villi and the cultured Tros transcript were found to express TSLP/TSLPR mRNA and secreted TSLP protein.In addition,TSLP receptor was also expressed on trophoblasts.Conclusion:Both TSLP and its receptor are expressed on villi and trophoblast cells,which suggests that TSLP plays an important role in maternal-fetal immuno-tolerance in human early pregnancy.

Objective:To investigate the current status of health-promoting lifestyle in patients with chronic low back pain and explore its influencing factors.Methods:Convenience sampling method was adopted to select patients with chronic low back pain who visited a ClassⅢ Grade A hospital in Guangzhou from March to October 2019 as the research object ( n=189) . All patients with chronic low back pain were investigated with the General Information Questionnaire, Health-Promoting Lifestyle ProfileⅡ, Social Support Rate Scale, Low Back Pain Knowledge Questionnaire and the Chronic Disease Self-efficacy Scale. Single factor analysis, Pearson correlation analysis, multiple linear regression analysis were used to explore the influencing factors of health-promoting lifestyle. Results:Among 189 patients with chronic low back pain, the total score of health-promoting lifestyle was (118.68±18.61) , and scores from high to low were stress management (2.59±0.62) , interpersonal support (2.47±0.67) , nutrition (2.35±0.25) , self-actualization (2.33±0.35) , exercise (2.04±0.35) , health responsibility (1.93±0.19) . Single factor analysis showed that there were statistical differences in health-promoting lifestyle scores among chronic low back pain patients with different ages, marital status, co-resident, education level, occupation, body mass index (BMI) and with or without chronic diseases ( P<0.05) . Correlation analysis showed that the health-promoting lifestyle of patients with chronic low back pain was positively correlated with social support ( r=0.358, P<0.01) , self-efficacy ( r=0.418, P<0.01) and disease knowledge ( r=0.369, P<0.01) with statistical differences. Regression analysis indicated that social support, disease knowledge, self-efficacy, age and educational level were the main factors affecting whether patients with chronic low back pain adopted a health-promoting lifestyle ( P<0.05) . Conclusions:Patients with chronic low back pain have poor health-promoting lifestyles, especially in exercise and health responsibility, and health-promoting lifestyles are affected by social support, disease knowledge and self-efficacy. Scientific, professional and personalized health guidance should be strengthened to improve patients ' awareness of disease development, treatment, and control, strengthen patients ' health responsibilities and improve the accuracy of exercise therapy. At the same time, we should provide multi-channel information acquisition methods, encourage family participation, meet patients ' health promotion needs, and enhance their disease management self-efficacy.