Objective Orthogonal design was employed to determine the best techniques in processing Carbonized Imperata Cylindrical. Methods UV was used to detect the absorption force and tannis in Carbonized Imperata Cylindrica by different kinds of processing methods. Results The best technique is to bark the drug for 4 minutes at 270 ℃. Conclusion The optimized processing technique was proved to be reasonable by the repeated test.

Objective To observe the effect and safety of emergent intracoronary stenting in acute myocardial infarction (AMI) complicated by acute pump failure.Methods Fifteen patients with anterier wall AMI and apparent signs of cardiac shock were (70.1?8.4) years old on average. Their mean blood pressures were less than 85/60 mm?Hg and the ejection fractions (EF) were less than 42% on echocardiography. All the cases were performed in plantation of stenting in left anterior discending branch (LAD), among which, 4 were in right anterior artery (RCA) and 3 in left circumflex (LCX). Before and after stenting, the changes of the patients′ blood pressures, heart rates and cardiac functions tested by ultrasonography were observed. Results In 15 patients, whose LADs were completely occlusive, after stenting, the coronary arteries gained perfect reperfusion. Compared before and after stenting, the blood pressures were increased significantly after stenting [SBP (106?11.8) vs (76.2?4.9)mm?Hg, P

Objective To evaluate the efficacy of fibercholedochoscopy for the removal of residual stones after a surgical choledochostomy. Methods Two hundred and twenty cases of cholelithiasis underwent fibercholedochoscopy through a surgically formed T tube fistulae for residual stones from Sept. 1993 to Feb. 2002. Results A total of 572 times of fibercholedochoscopy was performed with residual stones totally evacuated in 201 cases (91.4%). Complications developed in 84 cases with no mortality. Conclusion Postoperative fibercholedochoscopy through a T tube fistulae is less traumatic and effective remedy for postoperatively retained common bile duct stones.

BACKGROUND:The aim of spinal mobilization and spinal manipulation is to correct vertebral subluxation. However, facet joint pressures are not clear during these two therapies.
OBJECTIVE:To compare human lumbar facet joint pressures during simulated high-velocity, low-amplitude spinal manipulationversuslow-velocity, low-amplitude spinal mobilization.
METHODS:Totaly 12 adult fresh lumbar spinal specimens (T12-S2) were divided into two groups randomly. Parameters of simulated spinal mobilization (n=6): preload angle 15° (speed 3°/s), maximum angle 20° (speed 1°/s), with 9 N horizontal force to L5 spinous process. Parameters of simulated spinal manipulation (n=6): preload angle 15° (speed 3°/s), impulse angle 20° (impulse speed 33°/s), with 22 N horizontal force to L5 spinous process. Pressures of bilateral L4-5/L5-S1 facet joints were measured with Tekscan system.
RESULTS AND CONCLUSION:(1) During two spinal manipulative therapies (rotation to the right and then back to the neutral position), pressures of right facet joints decreased first and then increased gradualy, while pressures of left facet joints changed oppositely. (2) Pressures of right facet joints were similar regardless of manipulation type (P > 0.05). The maximum pressure of left facet joints was larger during manipulation than that during mobilization (P < 0.05). (3) Descending speed of pressures of right joint was larger during manipulation than that during mobilization (P < 0.01), and no significant difference in ascending speed of pressure of right facet joints was detected (P > 0.05). Both ascending and descending speeds of the left facet joints were larger during manipulation than that during mobilization (P < 0.01). (4) During two spinal manipulative therapies, pressures of ipsilateral facet joints decreased first and then increased, while pressures of contralateral facet joints increased first and then decreased. Joint pressure after treatment restored to that before treatment. (5) Impulse speed and magnitude of pressures of facet joints during manipulation were larger than that during mobilization. Facet joints are more possible to be injured during manipulation than that during mobilization. During manipulation, we should pay attention to the speed and intensity of the impact.

OBJECTIVES: To quantitatively analyzing the anatomic variants on temporal computed tomography (CT) in congenital external auditory canal stenosis (EACS), congenital aural atresia (CAA), and normal ear structure. METHODS: Through a retrospective study, we analyzed 142 temporal high-resolution CT studies performed in 71 microtia patients. The following 6 parameters were compared among the three groups: Marx classification, medial canal diameter, vertical facial nerve (VFN) anterior displacement, tegmen mastoideum position, tympanic cavity volume, and malleus-incus joint or malleus-incus complex (MIC) area. RESULTS: The results showed that the microtia distributions in the Marx classification in these three groups were significantly different, as 86% (31 of 35) of ears with major microtia (third-degree dysplasia) had an atresia, and in 54.8% (23 of 42) of the minor microtic (first-degree or second-degree) ears, the bony or cartilaginous part of the external auditory canal was stenotic. Measurement data also showed that the potential medial canal diameter of the atresia group was obviously shorter than that of the stenosis group. The VFN anterior displacement and temporomandibular joint backward-shift together lead to medial canal diameters in ears with atresic canals that is smaller than those with stenotic canals. The tegmen mastoideum position was not significantly different between the three groups. CONCLUSION: The mal-development of the external auditory canal is significantly associated with auricle and middle ear developmental anomalies. Compared with CAA ears, EACS have better development of the auricle, canal, tympanic cavity and MIC and relatively safer surgical operation except for the position of the tegmen mastoideum and the VFN.
Classification ; Constriction, Pathologic* ; Ear ; Ear Canal ; Ear, Middle ; Facial Nerve ; Humans ; Joints ; Multidetector Computed Tomography ; Retrospective Studies ; Temporomandibular Joint

Objective To explore the application value and clinical effect of the bi-directional quality control module for Surgical Package in artificial femoral head replacement. Methods A total of 360 patients undergoing artificial femoral head replacement in our hospital from June 2015 to June 2017 were selected and divided into observation group and control group, 180 cases in each group. The observation group,used bi-directional quality control module of surgical bag, while the control group used conventional management model of surgical bag . The operation time, the incidence of surgical bag, postoperative complications and postoperative satisfaction were compared. Results The average operation time in the observation group was (69.2 ± 11.6)min, the average operation time in the control group was (76.8 ± 14.5)min, P<0.01. The total defect rate in the observation group was 2.2% . The total defect rate in the control group was 22.2%, P<0.01; the observation group had less severe pain, incision split, fever, postoperative bleeding, incision infection than the control group, P<0.05. Patient satisfaction score was (89.38±7.83) points in the observation group, (79.18±5.55) points in the control group, P<0.01. Conclusions The bi-directional quality control module of surgical package can shorten the operation time, reduce the defect surgical packages, reduce the incidence of postoperative complications , improve the quality of operation, and improve patient satisfaction in the application of artificial femoral head replacement. It is worthy of clinical application.

OBJECTIVE: To explore the relationship between three-dimensional (3-D) reconstruction of regenerated fibers and functional recovery after mastoid segment of facial nerve was repaired with either end-to-end anastomosis or autogenous great auricular nerve grafting. METHOD: Thirty healthy adult New Zealand white rabbits were randomly divided into two groups: one was end-to-end anastomosis group and another was autogenous great auricular nerve grafting group. Only mastoid segment of right facial nerve of each animal was dissected and the contralateral nerve was as a control. Electromyogram (EMG) were recorded from 5 animals of each group at the 30th d, 90th d and 120th d after operation. After that the nerves were extracted, fixed, decalcified and embedded in paraffin. Samples was sectioned serially at 6 microm and stained with special trichrome stain. All the imagines were imported into Mimics software to reconstruct the 3-D model. RESULT: The significant differences were found in the regenerate fibers on 30 th d,and were found in amplitude of EMG on 30 th d and 90 th d. The image of 3-D reconstruction showed that the myelin sheath were thickening, connected from proximal to the distal gradually after repair. CONCLUSION The 3-D reconstruction of regenerated nerve fibers partly conformed to the functional recovery after facial nerve trunk was repaired. The functional recovery of facial nerve was related with both the quantity and the quality of regenerated nerve fibers.
Anastomosis, Surgical ; Animals ; Facial Nerve ; physiology ; surgery ; Image Processing, Computer-Assisted ; Male ; Mastoid ; innervation ; Nerve Regeneration ; Rabbits ; Reconstructive Surgical Procedures ; rehabilitation ; Recovery of Function

Objective To understand the human immunodeficiency virus(HIV) infection status and features of sexual behavior among men who have sex with men (MSM) and receive voluntary counseling and serological testing appointed through a web-based registering system to analyze the related factors.Methods The MSM who have homosexual behavior in nearly a year were recruited for HIV,syphilis and hepatitis C virus (HCV) antibody assay in peripheral blood and a face-to-face questionnaire survey was conduct among them to obtain the information about their demographic characteristics and sexual behavior.Binary logistic regression model was used to identify the risk factors associated with HIV infection.Results A total of 400 MSM were enrolled in our study,the median age is 27 years old,the unmarried MSM accounted for 65.25％,56.00％ of them had high education level and the awareness rate of knowledge regarding HIV was 92.75％.The percentages of using condom at latest homosexual or heterosexual behavior were 78.96％ and 79.82％.The HIV incidence was 7.25％ (29/400).Age,condom use at each homosexual or heterosexual behavior,the awareness rate of knowledge regarding HIV,sexually transmitted disease (STD) infection,Treponema pallidum infection were associated with HIV infection.Conclusions MSM receiving web appointed HIV counseling and testing had high prevalence of risk behaviors and high HIV infection rate,but the rate of condom use was relatively low.It is necessary to strengthen the promotion of HIV test through web based appointment.The integrated mechanism for the prevention and control of STD and AIDS should be improved and effective target behavior intervention in MSM should be strengthened in our intervention programs.

Cardiovascular disease is still the world's first cause of death,its morbidity and mortality rates are still increasing. Recent studies have shown that circular RNA is involved in the regulation of gene expression and plays a significant role in the pathogenesis of cardiovascular diseases. Circular RNA is a kind of special non-coding RNA, which can regulate the expression of genes at the level of post-transcription or transcription,then regulate various life activities as well as cardiovascular system diseases,becoming a latest research hot spot in the field of RNA. Nowadays,more and more attention is paid to the role of cyclic RNA in the development, early diagnosis, disease monitoring and other aspects of cardiovascular diseases. In this paper,we make an overview of the latest research on circRNAs from the aspects of genesis, function,and the relationship with cardiovas cular diseases,and so on.

The objective of this study is to investigate the effect of chemokine CXCR4 on the apoptosis of gastric cancer cells through IL-6/STAT3 signaling pathway and to explore the related mechanisms.Fluorescence quantitative PCR and Western blot were performed to detect the mRNA and protein expression levels of the chemokine CXCR4 in human gastric cancer tissues and adjacent non-cancerous tissues(PCT).Next,CXCR4 knockdown and overexpression were achieved by transfecting SGC7901 gastric cancer cell line with lentiviral vectors.TUNEL staining was used to evaluate the apoptosis of SGC7901 cells,while MTT assay was employed to measure cell proliferation.Western blot was conducted to determine the expression of apoptosis-related proteins Bax and Bcl2.Further,enzyme-linked immunosorbent assay(ELISA)was employed to measure the secretion levels of inflammatory cytokines.Real-time fluorescent quantitative PCR(RT-PCR)was utilized to quantify the expression of IL-6 mRNA in the IL-6/STAT3 signaling pathway,and Western blot was performed to analyze the expression of STAT3-Ser727 protein.In addition,after knocking down CXCR4 in SGC7901 cells,IL-6/STAT3 signaling pathway agonist lipopolysaccharide(LPS)was transfected,while in CXCR4-overexpressing SGC7901 cells,IL-6/STAT3 signaling pathway inhibitors angoline or bruceantinol were transfected.Then TUNEL staining was used to assess cell apoptosis,and Western blotting was performed to examine the expression levels of apoptosis-related proteins Bax and Bcl2 in these cells.Data showed that the expression of immune chemokine CXCR4 was increased in gastric cancer tissues,as compared with adjacent non-cancerous tissues.Single-cell gel electrophoresis analysis indicated that knockdown or overexpression of CXCR4 do not induce DNA damage in SGC7901 cells.TUNEL staining,MTT cell proliferation assay and Western blotting demonstrated that knockdown of CXCR4 in SGC7901 cells promoted the apoptosis in SGC7901 cells,while overexpression of CXCR4 inhibited the apoptosis.ELISA showed that knockdown of CXCR4 in SGC7901 cells promotes the expression of pro-inflammatory factors IL-1β and TNF-α,while inhibited the expression of anti-inflammatory factors IL-10 and TGF-β.Conversely,overexpression of CXCR4 demonstrated opposite effects.Finally,the activation of the IL-6/STAT3 signaling pathway significantly reduced the apoptosis induced by knocking down CXCR4 in iSGC7901 cells,whereas the inhibition of IL-6/STAT3 signaling pathway can significantly suppressed the induction of SGC7901 cells proliferation induced by CXCR4 overexpress.In conclusion,immunochemokine CXCR4 regulates gastric cancer cell apoptosis and inflammatory cytokines secretion through IL-6/STAT3 signaling pathway.