1.Progress in study on toxin of Conus textile
Xiao-Ling, LI ; Qiu-yun, DAI ; Pei-tang, HUANG
Bulletin of The Academy of Military Medical Sciences 2001;25(1):67-70
Conus textile is a kind of highly toxic and abundantly existing conus in the South China Sea. The toxin from C.textile could act on sodium channels(δ-conotoxins)and calcium channels (ω-,ε-conotoxins), respectively. Their specific chemical structure and biological activity have attracted a lot of attention in recent years. This article briefly reviews their biochemical characteristics, isolation, gene cloning, biological and neuropharmacological activities, as well as their potential applications.
2.Knockdown of ClC-2 Gene Expression Inhibits the Growth of BT-325 Human Glioma Cells
Xiang-Yun YANG ; Xiao-Gang LAI ; Yong ZHANG ; Jianming PEI ; Angang YANG ; Shisheng ZHOU
Chinese Journal of Rehabilitation Theory and Practice 2006;12(5):378-380
ObjectiveTo observe the growth of BT-325 human glioma cells after interfering volume-regulated chloride channel ClC-2 gene.MethodsTwo expression recombinant vectors of ClC-2 gene were designed and constructed. The primary plasmid, pSUPER.puro-shRNA, and the two recombinant plasmids, pSUPER.puro-shRNA-ClC-21 and pSUPER.puro-shRNA-ClC-22, were transfected into BT-325 cells by LipofectamineTM2000 (Groups: control, PP1 and PP2, respectively). The mRNA expression of ClC-2 gene was detected with reverse transcription polymerasse chain reaction (RT-PCR), the cellular survival was determined with MTT assay, and the cell cycle was measured with flow cytometry (FCM). ResultsClC-2 mRNA expression and the growth of the cells in PP1 and PP2 groups were significantly lower than that of control group. The cell cycle progression was blocked in G1 phase (PP1 and PP2 vs control,P<0.01). ConclusionThe growth of BT-325 human glioma cells is prevented by knockdown of ClC-2 gene expression, which may be one of the novel targets to inhibit growth of human malignant glioma cells.
3.Significance of COX2 and beta-catenin expression in nephroblastoma.
Xiao-Li HU ; Lin-Sheng ZHAO ; Lan-Yun SONG ; Pei-Ru NING
Chinese Journal of Pathology 2008;37(10):698-699
Child
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Child, Preschool
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Female
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Gene Expression Regulation, Neoplastic
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Humans
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Infant
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Male
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Wilms Tumor
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genetics
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metabolism
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Wnt Proteins
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genetics
;
metabolism
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beta Catenin
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genetics
;
metabolism
4.Epigastric heteropagus twins: report of a case.
Xiao-li HU ; Lan-yun SONG ; Lin-sheng ZHAO ; Pei-ru NING
Chinese Journal of Pathology 2009;38(7):491-492
Abnormalities, Multiple
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pathology
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surgery
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Humans
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Infant, Newborn
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Male
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Twins, Conjoined
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pathology
;
surgery
6.Carcinoid tumor of common bile duct: report of a case in pediatric patient.
Lan-yun SONG ; Xiao-li HU ; Lin-sheng ZHAO ; Pei-ru NING
Chinese Journal of Pathology 2011;40(1):54-55
Adenocarcinoma
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metabolism
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pathology
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Carcinoid Tumor
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metabolism
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pathology
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surgery
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Child
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Chromogranin A
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metabolism
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Common Bile Duct
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pathology
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surgery
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Common Bile Duct Neoplasms
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metabolism
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pathology
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surgery
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Diagnosis, Differential
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Duodenum
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pathology
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surgery
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Gallbladder
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pathology
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surgery
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Humans
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Keratins
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metabolism
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Lymphoma
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metabolism
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pathology
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Male
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Mucin-1
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metabolism
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Neoplasm Invasiveness
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Rhabdomyosarcoma
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metabolism
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pathology
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Stomach
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pathology
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surgery
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Synaptophysin
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metabolism
8.Nephroblastomatosis and nephroblastoma: report of a case.
Xiao-li HU ; Lan-yun SONG ; Lin-sheng ZHAO ; Pei-ru NING ; Li ZHAO
Chinese Journal of Pathology 2013;42(12):841-842
Humans
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Infant
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Kidney Neoplasms
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metabolism
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pathology
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surgery
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Male
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Mucin-1
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metabolism
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Nephrectomy
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Precancerous Conditions
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metabolism
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pathology
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surgery
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Vimentin
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metabolism
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WT1 Proteins
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metabolism
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Wilms Tumor
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metabolism
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pathology
;
surgery
9.Study of intraocular pressure diurnal variation curves in patients with open-angle glaucoma and normal subjects
Hong-Min YUN ; Pei FU ; Jin-Song YUAN ; Bin ZHANG ; Xiao-Xin LI ;
Ophthalmology in China 1993;0(01):-
Objective To observe the diurnal variation of intraocular pressure(IOP)measured with Goldmann applanation tonomoter(GAT)in patients with primary open angle glaucoma(POAG),normal tension glaucoma(NTG)and healthy controls,and to compare the differences of diurnal variation curves between two eyes of each subject and define the distribution of the peak of IOP. Design Prospective case series.Participants POAG patients,NTG patients and healthy controls.Methods The diurnal variation of IOP in the two eyes of each of 30 POAG patients,30 NTG patients and 30 normal controls were investigated and the distributions of the peak IOP were observed.Main Outcome Measure Intraocular pressure.Results Measured with GAT,6.7% right eyes and 10.0% left eyes of normal people,20.0% right eyes and 23.3% left eyes of NTG patients,and 23.3% right eyes and 20.0% left eyes of POAG patients showed peak IOP at the time points of out-office period.Conclusions POAG patients,NTG patients and normal controls have asymmetric IOP diurnal variation.The two eyes of one individual cannot always be treated as the same.The IOP peak can occur at the time point during the out-office time.Measure IOP in office-time only may miss the dynamic information of IOP.
10.The influences of hepatitis B virus precore and basic core promoter region mutations on the immune responses of specific cytotoxic T lymphocytes
Xiao-Juan YANG ; Guo-Rong WU ; Hao PEI ; Jin-Juan QIAN ; Rui-Yun JI ;
Chinese Journal of Infectious Diseases 2007;0(12):-
Objective To investigate the influences of mutation at precore and basic core promoter(BCP) region in hepatitis B virus(HBV) on the immune response of specific cytotoxic T lymphocytes(CTL) in patients with chronic hepatitis B(CHB).Methods The number of specific CTL in peripheral blood mononuclear(PBMC) of CHB patients were tested by cytokine flow cytome- try(CFC) and HBV core18-27 peptide.HBV precore and BCP fragments were directly sequenced. Results Twenty-one(38.9%) samples were HBV precore G1896A mutation.Twenty-six(48.1%) samples were BCP region 1762/1764 combined mutation.Thirteen(24.1%) stains were three sites mutated simultaneously.Stimulated with HBV core 18-27 in vitro,the specific CTL level was signifi- cantly higher in the patients with G1896A mutation and BCP region mutation [(0.41?0.09)%, (0.36?0.08)%,(0.48?0.08)%,respectively]than those without mutation[(0.11?0.06)%, P