Objective:To study the incidence,risk factors and clinical manifestaion for ectopic pregnancy(EP)after in vitro fertilization and embryo transfer(IVF-ET). Methods:A retrospective study of IVF and intracytoplasmic sperm injection (ICSI) pregnancies was performed between January 2002 and July 2005. Results:A total of 668 woman conceived after IVF or ICSI were studied. The rate of EP was 8.2%,corresponding with 55 Eps,of which 15 were heteropic pregnancy(HP). There were 40 Hps in tubal facter group,There apper to be no relationship between d2 ET and d 3 ET,ovulation induction and age. Conclusions:Tubal pathology is associated risk of EP.

Objective: To study clinical significance of endogenous vitamin C 、 E in patients With hypertension and stroke. Methods: The serum vitamin C、 E, Malondialdehyde (MDA), superoxide dismutase (SOD) were determined by ultraviolet spectrophotometry in patients with hypertension (23 cases)、 hypertensive cerebral hemorrhage (CH, 30 cases)、 ischemic cerebrovascular disorders (IC, 25 cases) and control groups (20 cases). Results: Compared with control group, at acute stage of stroke, the Vit C、 E and SOD levels significantly reduced (P＜0. 01), the MDA level significantly increased (P＜0.01); at convalescent stage, the Vit C、 E gradually recover to normal, the SOD still reduced (P＜0.05), the MDA still incresed (P＜0.05). Conclusion: The endogenous Vit C、 E have significant change in patients with acute stage of stroke, it gradually recovers to normal in convalescent stage. Meanwhile the SOD and MDA change accordingly.

Intracoronary effects of calcitonin gene related peptide (CGRP) on coro-nary hemodynamics were observed in the normal and following different extent of coro-nary stenosis using graded coropary- stenosis modl in dogs. The results showed that CGRPincreased the coronary artery blood flow (CBF) and distant coronary blood prepsure (DCP),but decreased mean artery pressure (MAP), coronary vessels total resistance (R_T),large co-ronary vessels, resistance (R_L) and small coronary vessels resistance (R_s). Thirty minutesafter. coronary middle. stenosis, CGRP was given intracoronary, MAP had no significantchange, but CBF insreased and R_T R_L, R_s decreased, and which maintained for 30 min. 30min after coronary severe stenosis, CGRP increased CBF and MAP, but decreased DCP,R_T, R_L, R_S continuously. These results suggested that CGRP could ameliorate myocardialischemia through enlargement of coronary vessels and by increasing CBF.

The article reviews the biologica l characteristics of stem cells and discusses the possibility of utilizing stem ce lls from various origins for diabetes therapy.

Objective To observe the change of Wnt-β-catenin signaling's location and expression in kidney repair after acute kidney injury induced by ischemla reperfusion (I/R).Methods Ischemia reperfusion injury in BAT-gal reportor mice was made and blood sample was taken from tails on the 1th day after injury. Mice were sacrified on the 2th or 7th day and kidneys and blood were collected. Renal pathological change was observed by PAS stain. The changes of location and expression of Wnt-β-catenin signaling were detected by immunofluorescence costainning X-gal-LTL, X-gal-NKCC2, X-gal-DBA respectively. The protein expressions of the Wnt4 and co-receptor Lrp6 were assessed by Western blotting. Results PAS-stained kidney sections showed desquamative or flattened epithelia, necrotic debris on day 2 and regenerating tubules on day 7. An injury-induced enhancement of the Wnt pathway response (X-gal staining) in kidney cortex and out-medulla. Immunolabelling of kidney sections from injured BAT-gal mice revealed that X-gal staining was detected in kidney epithelial cells (double-labelled with LTL or NKCC2). Western blotting showed the Wnt4 protein was up-regulated and phospho-Lrp6, indicative of active canonical Wnt signaling, was noted in kidney cortex from day 2 after I/R, but in control kidney cortex pLrp6 was not detected. Conclusion Wnt-β-catenin signaling is activited after acute kidney I/R injury and is required for tubular epithelial repair and regeneration following kidney I/R injury.

Based on the pathogenesis and symptom of rheumatoid arthritis (RA), professor LIN Guohua's unique opinion and method for RA in clinical treatment are summarized and analyzed. In the opinion of Professor Lin, RA is considered as "Jinbi" and "Gubi" in TCM, which is caused by deficient root with superficial excess. Based on the symptoms of RA, attention should be focused on lung-kidney diagnosis and treatment, and gold and catgut implantation at acupoint can be mutually combined, which is aimed to provide a special and effective method for clinical treatment of RA.
Acupuncture Points ; Arthritis, Rheumatoid ; therapy ; Female ; Gold ; therapeutic use ; Humans ; Middle Aged ; Prostheses and Implants

Drugs, Chinese Herbal ; chemistry ; therapeutic use ; Female ; Humans ; Lung Neoplasms ; drug therapy ; pathology ; radiotherapy ; Male ; Middle Aged ; Phytotherapy ; Radiation Injuries ; prevention & control ; Spinal Neoplasms ; drug therapy ; secondary ; Treatment Outcome

Objective To set up care service quality evaluation indicators and establish weight coefficient for assessment of elderly institution in Chongqing.Methods SEVQUAL Model was used as the theoretical guidance,through Delphi expert inquiry,analytic hierarchy process (AHP),the weight of each indicator in five grades and consistency check was calculated.Results The care serv ice quality evaluation indicators contained five first-grade indexes and twenty second-grade indexes.The first-grade indexes included tangibles,reliability,responsiveness,assurance,empathy,respectively.The results of the consisten-cy check of the single grade sorts had a satisfying consistency(CR＜0.10).Conclusions The scientific meth-ods and comprehensive indicators were applied to the construction of care service quality e-valuation indicators for elderly institution in Chongqing.In some degree,this construction of indicators may improve quality of care service and provide useful reference evidence for quality assessment in elderly in-stitution.

Objective To investigate whether the effect of PBL is better than LBL when used in a new type of three phases labor trainee teaching. Methods 216 medical students of the five-year and seven-year program who took part in labor trainee in the 2013-2014 school year were chosen and divided into two groups:PBL group and LBL group. Both the two groups' labor trainee were performed as three phases: trainee preparation, delivery room trainee, and discussion-class after trainee. Ques-tionnaire investigations were used to evaluate the effect of the delivery room trainee and discussion-class in PBL group and LBL group. A quiz after the third phase was used to examine whether the teaching effect of the two groups is different or not. Statistical analysis was performed using SPSS 16.0 software. Count data between sample rates were compared by chi square test, mean data between the two groups were compared by t test, the inspection level is α=0.05. Results Both in the delivery room trainee and discussion-class after trainee, questionnaire score in PBL group was significantly higher than that in LBL group (P<0.05). The average of quiz score in PBL group was 25.7±2.1, which was significantly higher than that in LBL group (19.3±3.6) (P<0.05). The excellent rate in PBL group was 82.6%, which was also higher than that in LBL group (P<0.05). Conclusion PBL teaching method elevates learning initiative of medical students and increases the teaching effect, and it can be promoted in labor trainee teaching.

The active form of vitamin D,1,25-Dihydroxyvitamin D3 \,is a secosteroid hormone that binds to the vitamin D receptor(VDR),a member of the superfamily of nuclear receptors for steroid hormones,thyroid hormone,and retinoic acid.1,25-Dihydroxyvitamin D3 and its analogue regulate calcium and bone metabolism,control cell proliferation and differentiation,and exert immunoregulatory activities.Recent advances in understanding their functions and novel insights into the immunomodulatory mechanisms they control suggest a wider applicability in the treatment of autoimmune diseases and induction of allograft tolerance.In addition to direct effects on T cell activation,1,25-Dihydroxyvitamin D3 and its analogue modulate with different mechanisms the phenotype and function of antigen-presenting cells(APC),and,in particular,of dendritic cells(DC).In vitro and in vivo experiments have shown that 1,25-Dihydroxyvitamin D3 and its analogue induce DC to acquire tolerogenic properties that favor the induction of regulatory rather than effector T cells.These intriguing actions of 1,25-Dihydroxyvitamin D3 and its nanlogue have been demonstrated in several experimental models and could be exploited,in principle,to treat a variety of human autoimmune diseases,or inhibit allograft rejection.