Pancreatic cancer has a strong ability of invasive and metastasis,and the mechanism is still not completely clear.It has been demonstrated that aberrant expression of microRNAs(miRNA) is closely associated with the occurrence,development and metastasis of pancreatic cancer.The reveal of miRNA mechanism related to the metastasis of pancreatic cancer would provide a new approach to the treatment of pancreatic cancer.

Objective To observe the hypoglycemic, hypolipidemic and antioxidant effects of Zhuye Shigao Decoction (ZSD) on experimental type 2 diabetes mellitus rats. Methods Type 2 diabetes mellitus rat model was induced by intragastric administration of high fat diet(10 mL/kg) and intraperitoneal administration of streptozotocin (30 mg·kg-1). Sixty male Sprague-Dawley rats were randomized into normal group, model group, low-, moderate-and high-dose ZSD groups (in the dosage of 3.6, 7.2, 14.4 g·kg-1·d-1 respectively), and Metformin group (0.15 g·kg-1·d-1) , 10 rats in each group. With fasting blood glucose, urine glucose, glycosylated hemoglobin, total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol(LDL-C), serum insulin, serum superoxide dismutase(SOD) and malondialdehyde (MDA) as the main indexes, we evaluated the influence of ZSD on the blood glucose and lipid levels as well as oxidative stress in type 2 diabetes mellitus rats. Results Compared with the model group, the levels of blood glucose, urine glucose and glycosylated hemoglobin, and the contents of TC, TG, MDA were decreased (P <0.05), whereas SOD activity and serum insulin level were obviously increased (P < 0.05) in ZSD groups. ZSD showed dose-related effectiveness in the treatment of diabetic rats. Conclusion ZSD has certain hypoglycemic, hypolipidemic and antioxidant effects in treating type 2 diabetes mellitus.

Objective To analyze the risk factors for traumatic cerebral venous sinus occlusion (CVSO)and to investigate the strategies of early diagnosis of traumatic CVSO. Methods The clinical data of 212 patients with moderate to severe closed traumatic brain injury from January 2012 to December 2015 were analyzed retrospectively. Logistic regression analysis was used to evaluate the risk factors for traumatic CVSO. Results Of the 212 patients with traumatic brain injury, 16.5%(35/212) patients had CVSO. Ten patients had CVSO of thrombotic type (typeⅠ), 16 patients had CVSO of compression type (typeⅡ), and 9 patients had CVSO of mixed type (typeⅢ). Logistic regression analysis showed that skull fracture (OR = 8.141; 95%CI: 3.224-20.840) and epidural hematoma of crossing venous sinus (OR = 3.179; 95%CI: 1.470-7.037) were the risk factors for CVSO, and the former was more significantly correlated with CVSO. Female gender was the risk factor for CVSO typeⅠ(OR =10.425; 95%CI: 1.831-30.053), epidural hematoma of crossing venous sinus was the risk factor for CVSO typeⅡ(OR = 5.766; 95%CI: 1.885-18.197), and skull fracture, epidural hematoma of crossing venous sinus, and the previous history of vein thrombosis was the risk factors for CVSO type Ⅲ(OR =18.005, 4.596, 11.394; 95%CI: 2.021-58.836, 1.144-19.525, 1.436-46.558). Conclusions In the early diagnosis of traumatic CVSO, the crossing venous sinus fracture line and epidural hematoma should be given attention. Attention should be paid to the history of venous thrombosis. MR venography and CT venography contributes to early diagnosis of CVSO.

Humans ; Lymphoma, Large B-Cell, Diffuse ; Societies, Medical ; United States

Objective To evaluate the safety of CT guided percutaneous lung biopsy in the elderly,and to analyze the risk factors for its complications.Methods Totally 204 elderly patients aged ≥65 years underwent transthoracic needle biopsy under CT guidance.Clinical data,complication and its risk factors were analyzed retrospectively.Results The complications included pneumothorax accounting for 15.2％ (31/204),hemoptysis 21.6％ (44/204),and no mortality was found.Logistic regression analysis showed that needle path ≥5.00 cm,lesion size ≤2.00 cm and emphysema around the lesion were the independent risk factors for postoperative pneumothorax (OR=4.05,2.54 and 3.97,all P＜0.05).Meanwhile,the enhancement degree by CT ＞40 HU,needle path ≥5.00 cm and lesion size ≤2.00 cm were the independent risk factors for postoperative bleeding (OR=2.27,2.82 and 2.33,all P＜0.05) in elderly patients undergoing lung biopsy.The sensitivity and specificity for postoperative pneumothorax and bleeding obtained by forecasting equation were 87.1％ and 49.1％,54.5％ and 80.8％ respectively.Conclusions The length of needle path,lesion size and emphysema around the lesion are correlated with pneumothroax,and length of needle path,lesion size and the CT enhancement degree are correlated with bleeding after percutaneous lung biopsy in the elderly.

AIM: To explore the effect and the mechanism of sulphated heparin on the proliferation and the apoptosis of human hepatocellular carcinoma cells. METHODS: The human hepatocellular carcinoma cell line (HepG-2) was used to identify the expression of ras gene protein and to study the effect of sulphated heparin on proliferation and the apoptosis in vitro . RESULTS: The sulphated heparin downregulated the ras protein expression and inhibited the cell growth in HepG2 cells. In the presence of sulphated heparin, the apoptosis rate of HepG2 increased. CONCLUSION:The data suggest that the effects of sulphated heparin on the proliferation and the apoptosis of the human hepatocellular carcinoma cell are correlated with the signaling transduction mediated by ras gene protein.

Objective To explore the clinical manifestations and the characteristics of images of indocyanine green angiography (ICGA) and fundus fluorescein angiography(FFA) of multifocal choroiditis. Methods Eight patients (10 eyes) with multifocal choroiditis were gathered. The clinical manifestations and the images of ICGA and FFA were analyzed. Results Foci of multifocal choroiditis were found in posterior pole and peripheral areas of ocular fundi of all of the 10 eyes. The images of ICGA revealed hypofluorescence in focal area. The images of FFA showed hypofluorescence at the early phase and fluorescein leakage at the late phase in the active focus, and fluorescein staining and window defect fluorescence in the inactive focus. Conclusions The clinical manifestations of multifocal choroiditis varied with disease course, location and numbers of the lesions. ICGA and FFA can show the development of the disease clearly, which may guide the treatment.

Objective To investigate the CT and MRI features of solid-cystic papillary tumors(SCPTs) of the pancreas.Methods Eight cases of SCPTs confirmed by operation and pathology underwent both plain and contrast-enhanced CT examination,and MR examination in three cases and contrast-enhanced MRI in two cases.Results In the eight patients,the mean tumor diameter was 7.6 cm and the lesions were found mainly in the body and tail of the pancreas,only one at the pancreatic head.On plain CT scan,the tumors appeared as huge solid-cystic mass with definite margin and an integritied capsul,the solid areas were papillary or irregular components,and the cystic areas in central were low attenuation.On contrast-enhanced CT,the cystic wall and solid part were slight enhancement in arterial phase,and moderate or obvious enhancement in parenchymal phase.Irregular ringed and punctate calcification could be seen in five cases,the acute hemorrhage occurred in one case,slight dilation of the pancreatic duct was noticed in one patient.MRI showed an ovoid solid-cystic mass of the pancreas with distinct border.On T1WI,heterogeneous iso-and hypo-intensity was observed.One case showing the high signal intensity in tumor was subacute hemorrhage.On T2WI,the tumors were mixed hypo-,iso-and hyperintensity.The papillary or flocculent solid portion and the cystic wall were enhanced after gadolinium was used.Conclusion SCPTs is of certain clinic,CT and MRI characteristic findings,which can aid in reaching a proper diagnosis.

To investigate the changes in the activated T-lymphocyte CD3/HLA-DR and CD3/CD(16+56) populations in peripheral blood of the patients with allogeneic hand transplantation, lymphocytes from peripheral blood of the patients at different time points were immunologically labeled with dual color fluoresecent monoclonal antibodies CD3/CD(16+56) and CD3/HLA-DR, mono-color fluoresecent monoclonal antibody CD25. CD25, CD3/CD(16+56), and CD3/HLA-DR were determined with flow cytometry (FCM). The levels of activated T-lymphocyte (CD25 +,CD3 +/HLA-DR + ), silent T-lymphocyte [CD3 +/CD(16+56) -,CD3 +/HLA-DA - ] decreased significantly during the first week after transplantation and then increased gradually to the pre-operafive level. Nature killer cells [CD3 -/CD(16+56) +] increased significantly at the first day after transplantation, then decreased sharply and maintained a lower level. The results suggest that immunosuppressive agents have significantly effects on lymphocyte subsets in allogenaic hand transplanted patients, and dynamic determination of HLA-DR, CD3 /CD(16+56) could be valuable in immunomonitoring after allogeneichand transplantation.

To design and manufacture a new bone matrix material (NBM) composed of both organic and inorganic materials for bone tissue engineering, the osteogenic potential of NBM combined with osteoblasts was studied in vitro and in vivo. Osteoblasts from bone marrow stromal cells of New Zealand rabbit were cultured with NBM in vitro , then the materials combined with osteoblasts were implanted into the skeletal muscles of rabbits. The osteogenic potential of NBM was evaluated using contrast microscope, scan electromicroscope and histological examination. Osteoblasts could attach and proliferate well in the NBM, secrecting lots of extracellular matrix, while in vivo experiment of the NBM in osteoblasts group showed that a large number of lymphacytes and phagocytes invaded into the inner of the material in the rabbit skeletal muscle beds after 4 weeks of implantation, no new bone formation was observed after 8 weeks. The different osteogenic potential expressed by NBM between in vitro and in vivo may be due to the immunogenity of NBM which causes cellular immunoreaction to destroy the osteogenic environment. More attention should be paid to the immunoreaction problem in tissue engineering between the host and organic inorganic composite materials.