3.Celluar Immunity in Patients with Juvenile Rheumatoid Arthritis (JRA)
zheng-hai, QU ; pei-xuan, CHENG ; ke, SHI
Journal of Applied Clinical Pediatrics 1986;0(02):-
Objective To evaluate the change of cellular immunity and its clinical significance in JRA.Methods 7 lymphocyte swbpopulation was analyzed by immunofluorescein and interleukin 2 (IL-2) produced by peripheral blood mononuclear cells in vitro by MTT colorimetric assay. 29 times of various stage with JRA were examined, including 14 clinically active patients, 8 posttreatment or 7 clinically inactive ones. There are 19 healthy children of similar age in control group.Results In active patients, the number of OKT8, OKT4, the ratio of OKT4/OKT8 and the level of IL-2 decreased significantly compared with normal controls. These changes recovered matkedly in remission patients though they did alter affective treatment for (2~4) weeks.Conclusion Patients with active JRA are characterised by aberration of cellular immunity and the aberration reverses obviously slow in comparision with the clinical manifestetions and the routine laboratory investigation.
4.Intervention Effects of Granisetron Hydrochloride on Vasovagal Syncope in Rabbits
yan, DONG ; hong-wei, WANG ; ping, HUANG ; pei-xuan, CHENG ; zhi, XIA ; liang-pin, ZHANG
Journal of Applied Clinical Pediatrics 1992;0(05):-
Objective To study the effects of granisetron hydrochloride on vasovagal syncope(VVS) in rabbits.Methods Twenty-four healthy New Zealand rabbits were divided stochastically into control group and intervention group,12 in each group. The control group was injected intravenously with normal saline. The intervention group was injected intravenously with granisetron hydrchloiride.Rabbit VVS models were established,each was taken at 4 points in time in the bloodletting process:T1,T2,T3,T4,to compare the bloodletting time,the concentration of 5-hydroxytryptamine(5-HT) in T2,T3,T4 and the total blood volume between the groups,and monitor the heart rate, blood pressure during the entire process.Results 1.The time of intervention group in T2,T3,T4 was longer than the time of control group obviously(P
5.Experimental Study of Adrenomedullin in Autoimmune Myocarditis Induced by Immunization of Mice with Lactobacillus Casei Cell Wall Element
ji-wei, ZHANG ; hong-wei, WANG ; mei-zhen, NIU ; hong, SHI ; qing-jun, LIU ; pei-xuan, CHENG ; ya -li, LIU
Journal of Applied Clinical Pediatrics 2004;0(11):-
Objective To study adrenomedullin (AM) mRNA and protein expression level in myocardium of autoimmune myocarditis animal models induced by immunization of mice with lactobacillus casei cell wall element(LCWE). Methods Forty-five Balb/c male mice were randomly divided into experimental group (n = 30) and control group (n = 15), which were intraperitoneally injected with LCWE and phosphate buffered solution(PBS) at day 0,3,5 and 10,respectively. Sera and myocardium samples were gained 14,21 and 28 days after the first immunization. AM expression levels were determined by semiquantitative reverse transcriptase-polymerase chain reaction(RT- PCR) and immunchistochemistry,and mycardial histopathological lesions were observed. The anti- myosin antibodies in different stages were examined by an ELISA. Results There were myocardial necrosis or inflammatory infiltration in the experimental group, but myocardial lesions were not found in the control group. Anti - myosin antibodies were detected in sera of experimental mice,but not in control group. Immunchistochemistry findings demonstrated that AM expression level was higher in the experimental group than in the control group( P
6.Regulatory effect of procyanidins on the expressions of interferon-gamma, interleukin-12 and transcription factor T-bet mRNA in peripheral blood mononuclear cell of patients with alopecia areata.
Pei-Cheng ZHU ; Guo-Wei XUAN ; Da-Can CHEN
Chinese Journal of Integrated Traditional and Western Medicine 2008;28(10):900-902
OBJECTIVETo investigate the regulatory effect of procyanidins (PC) on the expressions of Th1 type cytokines (IFN-gamma and IL-12) and the transcription factor T-bet mRNA in peripheral blood mononuclear cell (PBMC) in patients with alopecia areata (AA).
METHODSAbove-mentioned expressions were detected using RT-PCR technique in 20 AA patients (8 of mild and 12 of severe degree) and 10 healthy subjects after phytoaemagglutinin (PHA) or PHA + PC stimulation.
RESULTSAfter being stimulated by PHA and PHA + PC, in patients with severe AA, the expression of T-bet mRNA was 0.581 +/- 0.148 and 0.419 +/- 0.113 respectively; that of IFN-gamma mRNA, 0.689 +/- 0.219 and 0.430 +/- 0.162; and that of IL-12 mRNA, 0.198 +/- 0.056 and 0.136 +/- 0.035, respectively. As compared with those before stimulation, the respective difference was statistically significant (P < 0.05).
CONCLUSIONSPC can inhibit the expressions of Th1 type cytokines and transcription factor T-bet in PBMC of severe AA patients, and reverse the Th1 responses. The authors offered that it maybe part of the acting mechanism of pine needle for treatment of AA.
Adolescent ; Adult ; Alopecia Areata ; drug therapy ; genetics ; immunology ; Case-Control Studies ; Cells, Cultured ; Gene Expression ; drug effects ; Humans ; Interferon-gamma ; genetics ; immunology ; Interleukin-12 ; genetics ; immunology ; Leukocytes, Mononuclear ; drug effects ; immunology ; Male ; Middle Aged ; Proanthocyanidins ; administration & dosage ; T-Box Domain Proteins ; genetics ; immunology ; Young Adult
8.Clinicopathologic study of aortic valves in children.
Ping HUANG ; Hong-wei WANG ; Zhen-lu ZHANG ; Xiu-fen HU ; Yan-ping LI ; Pei-xuan CHENG ; Jian-ying LIU
Chinese Journal of Pathology 2006;35(10):623-624
Adolescent
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Aortic Valve
;
abnormalities
;
Aortic Valve Insufficiency
;
complications
;
pathology
;
surgery
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Aortic Valve Stenosis
;
complications
;
pathology
;
surgery
;
Child
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Endocarditis
;
complications
;
pathology
;
surgery
;
Female
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Heart Defects, Congenital
;
complications
;
pathology
;
surgery
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Heart Valve Prosthesis Implantation
;
Humans
;
Male
;
Rheumatic Heart Disease
;
complications
;
pathology
;
surgery
9.The Emergence, Epidemiology, and Etiology of Haff Disease
Pei PEI ; Yan Xiao LI ; Shuang Shuang LU ; Zhe LIU ; Rui WANG ; Cheng Xuan LU ; Kai LU
Biomedical and Environmental Sciences 2019;32(10):769-778
Haff disease is a type of human rhabdomyolysis characterized by the sudden onset of unexplained muscular rigidity and an elevated serum creatine kinase level within 24 h after consuming cooked aquatic products.Here,we reviewed a previous study on Haff disease and summarized the clinical manifestations,epidemiological characteristics,and etiological data to confirm the incidence and global epidemiology of the disease and identify the most common seafood vectors.Future directions for Haff disease study will include further prospective etiological studies and the development of prevention and control strategies.
10.Expression of Matrix Metallproteinase-2 and Matrix Metallproteinase-9 on Aortic Valve in Children with Rheumatic Heart Disease
yan-ping, LI ; xiu-fen, HU ; hong-wei, WANG ; ping, HUANG ; pei-xuan, CHENG ; neng-bao, LIU ; shao-hua, ZHU ; xiao-heng, LI
Journal of Applied Clinical Pediatrics 2006;0(21):-
Objective To detect the expression of matrix metallproteinases(MMPs) in aortic valve of children who suffered from rheumatic heart disease(RHD) and to explore the pathological role of MMPs in children′s rheumatic aortic valve disease.Methods RHD group composed of 18 aortic valves from children suffered from RHD.Controls were 8 children who were died accidentally without cardiovascular system diseases.Hematoxylin and eosin stain observing the histological characteristic of the 2 groups.Immunohistochemistry was used to detect expression of MMP2 and MMP9 on aortic valves in 2 groups.Results Hematoxylin and eosin stain showed:in RHD the valves′ structure were destroyed along with fibrous tissue proliferation,mucinous degeneration,collagen and fiber hyalinization,blood vessel and blood capillary proliferation,lymphocyte,plasmocyte,monocyte infiltration.Immunohistochemistry showed that MMP2 and MMP9 expression were significantly higher than those in the aortic of RHD(68.85?13.08,64.35?9.59) compared with control group(107.31?23.39,116.28?6.99)(t=3.92,10.18 all P