The article summarized the teaching experience to cultivate the capability of writing the standardized diagnostic imaging reports in the internes of the medical imaging speciality.The need and content of diagnostic imaging reports was standardized.The skill to read image and the capability to write the standardized diagnostic imaging report was cultivated gradually by three phases composed of the accidence phase,and the improve phase and the practice phase.

OBJECTIVETaxus species are highly valued for the production of taxol. Based on high-throughput sequenceing, EST-SSRs were explored and studied in the transcriptome of Taxus cuspidata.

METHODT cuspidata leaf cDNA was extracted and sequenced by 454 GS FLX Titanium. High-quality sequences were assembled using Newbler Assembler Software, which produced unique sequences. SSRs motif was explored using simple sequence repeat identification tool (Perl Script). Primers were designed using PRIMER3.

RESULTA total of 81 148 high-quality reads from the needles of T. cuspidata were produced using the Roche GS FLX Titanium system. A total of 20 557 unique sequences were obtained. There were 753 simple sequence repeat motifs identified. Primers of PCR were obtained for 519 EST-SSRs, randomly selected cloning sequencing revealed that 87.5% of ESTs were the same as the results of Sanger sequencing.

CONCLUSIONThe results provide the first EST-SSRs collection in Taxus and are essential for future efforts of gene discovery, functional genomics, and genome annotation in related species.

DNA Primers ; genetics ; Expressed Sequence Tags ; Gene Expression Profiling ; methods ; Gene Expression Regulation, Plant ; High-Throughput Nucleotide Sequencing ; methods ; Microsatellite Repeats ; genetics ; Taxus ; genetics ; Transcriptome ; genetics

OBJECTIVETo study the expression of phosphatase and tensin homology deleted on chromosometen ten (PTEN) protein, a tumor suppressor gene in breast cancer and its correlation with p27(kip1) and cyclin D1 expression.

METHODSPTEN protein expression, p27(kip1) and cyclin D1 protein expression were detected by immunohistochemical method in paraffin sections from 61 women with primary breast cancer. PTEN protein expression was compared with clinico-pathologic parameters as related to p27(kip1) and cyclin D1.

RESULTSPTEN, being shown in the cytoplasm, was negative in 6.6% (4/61), reduced in 41.0% (25/61) and positive in 52.5% (32/61) samples. PTEN expression level was correlated with axillary lymph node status, loss of estrogen receptor stain, recurrence and metastasis. On univariate analysis, the disease-free survival rate of patients with higher PTEN expression (> 50% cells stained) was better than those with lower expression (P = 0.0101). However, there was no correlation between p27(kip1), cyclin D1 expression or PTEN expression.

CONCLUSIONPTEN, its lower expression being correlated with poor outcome of breast cancer patients, plays a prominent role in breast cancer. p27(kip1) or cyclin D1 may not be the primary downstream genes of PTEN in breast cancer.

Adult ; Aged ; Breast Neoplasms ; chemistry ; mortality ; pathology ; Cyclin D1 ; analysis ; physiology ; Cyclin-Dependent Kinase Inhibitor p27 ; Female ; Humans ; Immunohistochemistry ; Intracellular Signaling Peptides and Proteins ; analysis ; physiology ; Lymphatic Metastasis ; Middle Aged ; PTEN Phosphohydrolase ; analysis ; physiology ; Prognosis

OBJECTIVETo investigate the chemical constituents of Ehretia thyrsiflora.

METHODCompounds were isolated by using silica gel, Sephadex LH-20 and RP-C18 chromatography; their structures were elucidated by means of spectral data analysis.

RESULTSeven compounds were isolated and identified as methyl rosmarinate (1), caffeic acid (2), quercetin (3), kampferol (4), kaempferol 3-O-alpha-D-arabinoside (5), quercetin 3-O-alpha-D-arabinoside (6), and p-hydroxy benzoic acid (7).

CONCLUSIONAll these compounds were isolated from E. thyrsiflora for the first time. Compounds 2-7 were isolated from genus Ehretia for the first time.

Acetates ; analysis ; Boraginaceae ; chemistry ; Drugs, Chinese Herbal ; chemistry

Objective:To explore the effect of acknowledge, introduction, duration, explain and thank (AIDET) communication model on the awareness rate of health knowledge, self-perceived burden and quality of life of stroke patients in return visits.Methods:Using the convenient sampling method, a total of 300 stroke patients who were admitted to the First Affiliated Hospital of Zhengzhou University from January 2017 to December 2019 were selected as the research objects. According to the odd and even order of admission, they were divided into the observation group and the control group, with 150 cases in each group. The control group was given regular communication mode intervention, while the observation group was given AIDET communication model intervention. Hamilton Anxiety Scale (HAMA) , Hamilton Depression Scale (HAMD) , awareness rate of health knowledge, Self-Perceived Burden Scale (SPBS) score, the MOS Item Short From Health Survey (SF-36) score and nursing satisfaction were compared between two groups.Results:After intervention, HAMA and HAMD scores in the observation group were lower than those in the control group, and the differences were statistically significant ( P<0.05) . After the intervention, the awareness rate of health knowledge in the observation group was 96.67% (145/150) , which was higher than 83.33% (125/150) in the control group, and the difference was statistically significant ( P<0.05) . After the intervention, the total score of SPBS of the observation group was lower than that of the control group, and the difference was statistically significant ( P<0.05) . There was no statistically significant difference in the economic burden between the two groups ( P>0.05) . After the intervention, the SF-36 score in all dimensions and nursing satisfaction of the observation group were higher than those of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:AIDET communication mode can reduce the negative emotion and self-feeling burden of stroke patients in return visits and improve their awareness rate of health knowledge and nursing satisfaction.

Objective:To investigate the status quo of psychological characteristics of mindfulness, family concern and rehabilitation compliance of patients undergoing hip arthroplasty and to explore the internal relationships between psychological characteristics of mindfulness, family concern and rehabilitation compliance of patients undergoing hip arthroplasty in order to clarify the mediating effect of psychological characteristics of mindfulness.Methods:Using the convenient sampling method, a total of 101 patients who underwent hip arthroplasty in Department of Orthopedics in Peking University Shenzhen Hospital from July 2017 to June 2018 were selected as research objects. Five Facet Mindfulness Questionnaire (FFMQ) , Family Adaptability, Partnership, Growth, Affection, and Resolve (Family APGAR) Index and Rehabilitation Compliance Scale were used to conduct questionnaire surveys. The Pearson correlation analysis were used to analyze the correlations between rehabilitation compliance, family concern and psychological characteristics of mindfulness. Then a hypothetical model was established to analyze the mediating effect of psychological characteristics of mindfulness between family concern and rehabilitation compliance.Results:Finally, 90 valid questionnaires were collected. The total scores of FFMQ, Family APGAR Index and Rehabilitation Compliance Scale were respectively (115.01±8.99) , (7.27±0.84) and (5.27±0.92) . The psychological characteristics of mindfulness, family concern and rehabilitation compliance of patients with hip replacement were positively correlated ( r=0.562, 0.491; P<0.01) . The constructed mediation model showed that family concern had a positive predictive effect on rehabilitation compliance ( P<0.05) , psychological characteristics of mindfulness had a positive predictive effect on rehabilitation compliance ( P<0.05) , and psychological characteristics of mindfulness played a mediating role between family concern and rehabilitation compliance of patients with hip replacement. Conclusions:In this study, family concern, psychological characteristics of mindfulness and rehabilitation compliance of patients with hip replacement are all at a moderate level. Psychological characteristics of mindfulness of patients, family concern and postoperative rehabilitation compliance are positively correlated, and psychological characteristics of mindfulness has a mediating role between family concern and rehabilitation compliance. Medical staff can carry out mindfulness psychological interventions for patients with hip replacement to improve family concern and promote rehabilitation compliance of patients.

OBJECTIVETo summarize the experiences and results of ventricular septal myectomy concomitant mitral valve replacement (MVR) for obstructive hypertrophic cardiomyopathy (OHCM).

METHODSFrom January 2000 to June 2007, 22 patients of OHCM with moderate or severe mitral regurgitation underwent concomitant ventricular septal myectomy concomitant MVR. There were 20 male and 2 female patients. The age ranged from 28 to 51 years old with a mean of (36 + or - 5) years old. The left ventricular out tract gradient pressure (LVOTGP) was 55 to 120 mm Hg (1 mm Hg = 0.133 kPa), with a mean of (88.0 + or - 15.8) mm Hg. The manifestation of pre-operative UCG, intra-operative transesophageal echocardiography (TEE) and post-operative UCG in 10 d, 6 months and 1 year were compared and analyzed.

RESULTSOne patient died in hospital due to serious ventricular arrhythmias. The intra-operative TEE showed that the phenomenon of systolic anterior motion (SAM) of mitral valve disappeared in all patients. Twenty-one cases were followed-up. The intra-operative TEE and post-operative UCG in every period of all 21 cases survived indicated that the mean LVOTGP and interventricular septal thickness (IVST) decreased obviously (P < 0.01).

CONCLUSIONConcomitant ventricular septal myectomy concomitant MVR is an effective and safe treatment for OHCM with moderate or severe mitral regurgitation, the short and mid-term outcome is excellent.

Adult ; Cardiomyopathy, Hypertrophic ; complications ; surgery ; Female ; Heart Valve Prosthesis Implantation ; Humans ; Male ; Middle Aged ; Mitral Valve ; surgery ; Mitral Valve Insufficiency ; complications ; surgery ; Retrospective Studies ; Treatment Outcome ; Ventricular Septum ; surgery

OBJECTIVE: To explore the effects of exogenous transforming growth factor-beta 1 (TGFbeta1) on peripheral nerve regeneration after the peripheral nerve injury and if TGFbeta1 regulates the expression of basic fibroblast growth factor (bFGF) in the anterior horn motoneurons of spinal cord during regeneration. METHODS: Forty-eight rats were crushed on the right sciatic nerve and then randomly divided into 2 groups: TGFbeta1 group and NS group. In TGFbeta1 group, TGFbeta1 50 microL (0.1 microg/mL) was injected into the proximal nerve near to the crushed nerve and after the operation the injured leg was injected with equal TGFbeta1 whereas the NS was replaced in the NS group. The rats of each group survived for 3, 7, 14 and 21 days after the lesion. The bFGF expression in the anterior horn motoneurons of spinal cord was detected by immunohistochemistry (IHC). Semi-thin section and Fast Blue retrograde tracing were also performed with the rats surviving for 21 days to observe the regeneration of distal end in the injured right sciatic nerve. RESULTS: The number of bFGF immunoreactive positive motoneurons in TGFbeta1 group was obviously higher than that of the NS group (P < 0.05). In the distal sciatic nerve of the rats treated with TGFbeta1, the number and diameter of regenerating myelinated axons and the thickness of myelinated sheath were more than those of the NS group (P < 0.05). The number of motoneurons in spinal cord and neurons in dorsol root ganglia (DRG) labelled with Fast Blue in the NS group was obviously lower than in the TGFbeta1 group (P < 0.01). CONCLUSION Exogenous TGFbeta1 plays an important role in promoting the peripheral nerve regeneration; TGFbeta1 up-regulates the bFGF expression in the anterior horn motoneurons of spinal cord during the peripheral nerve regeneration.
Animals ; Female ; Fibroblast Growth Factor 2 ; biosynthesis ; genetics ; Male ; Motor Neurons ; metabolism ; Nerve Regeneration ; drug effects ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Sciatic Nerve ; injuries ; metabolism ; physiology ; Spinal Cord ; metabolism ; Transforming Growth Factor beta ; pharmacology ; Transforming Growth Factor beta1

Objective To investigate the role of Wnt/β-catenin signaling pathway in hyperbaric oxygen (HBO) therapy for hypoxic-ischemic brain damage(HIBD).Methods The neural stem cells (NSCs) of neonatal rats were randomly divided into control group and 8 treatment groups,then the treatment groups were cultured in the supernatant of brain homogenate to simulate micro-environment of HIBD and normal brain respectively.Through electroporation β-catenin siRNA and negative control plasmid was transfected into NSCs respectively,then HBO therapy was performed.Immunocytochemical staining was performed simultaneously in the 9 groups of NSCs on precoated chamber slides to detect the differentiation of NSCs.Quantitative reverse transcriptase(RT)-PCR was used to detect the relative content of Ngn1 mRNA and bone morphogenetic protein(BMP4) mRNA in the of NSCs.Western blot was used to detect the relative content of Ngn1 protein and BMP4 protein in the NSCs.Results In vitro,HBO alone promoted NSCs infected with negative control siRNA (ncNSCs) to differentiate into neurons and oligodendrocytes and depressed astrogliosis ; HIBD or normal brain tissue extract cultures promoted ncNSCs to differentiate into neurons and oligodendrocytes but depressed astrogliosis,and the effect of HIBD brain extract cultures was superior to the latter and could be further increased by HBO; β-catenin siRNA decreased the NSE-positive neurons and increased glial fibrillary acidic protein-positive astrocytes in the siNSCs (NSCs infected with β-catenin siRNA) in vitro,the effect could not be inversed by HBO,but could be alleviated; HBO increased the level of Ngn1 mRNA and Ngn1 protein,decreased BMP4 mRNA and BMP4 protein of ncNSCs,transfection of β-catenin siRNA could down-regulate the expression of Ngn1 mRNA and upregulate BMP4 mRNA of NSCs in vitro respectively.Conclusions HBO can promote NSCs cultured with HIBD brain extract cultures to differentiate into neuronal or Oligodendrocyte,and inhibit them to differentiate into astrocytes.HBO therapy promotes the proliferation of NSCs in vitro,an effect which is correlated with β-catenin protein.HBO therapy can promote neurogenesis by β-catenin-induced activated Ngn1,and repress astrocytogenesis by β-catenin-induced down-regulated BMP4.There are potential cooperative actions of BMP4 and Ngn1 on differentiating rat NSCs in cerebral ischemic brain.The ability of Ngn1 to promote neurogenesis may allow Ngn1 to act as a potent neuronal commitment factor.

An open-access microfluidic chip which enabled automatic cell distribution and complex multi-step operations was developed.The microfluidic chip featured a key structure in which a nanoporous membrane was sandwiched by a cell culture chamber array layer and a corresponding media reservoir array layer.The microfluidic approach took advantage of the characteristics of the nanoporous membrane.On one side, this membrane permitted the flow of air but not liquid, thus acting as a flow-stop valve to enable automatic cell distribution.On the other side, it allowed diffusion-based media exchange and thus, mimicked the endothelial layer.In synergy with a liquid transferring platform, the open-access microfluidic system enabled complex multi-step operations involving medium exchange, drug treatment, and cell viability testing.By using this microfluidic protocol, a 10 × 10 tissue arrays was constructed in 90 s, followed by schedule-dependent drug testing.Morphological and immunohistochemical assays results indicated that the resultant tumor tissue was faithful to that in vivo.Drug testing assays showed that the microfluidic tissue array promised multi-step cell assays under biomimetic microenvironment, thus providing an advantageous tool for cell research.