1.The monitor report of Kashin-Beck Disease prevalence ra te in Changdu of Tibet
Jiang, LI ; Zhen-Qun-Pei, DING
Chinese Journal of Endemiology 2001;20(2):130-131
Objective To monitor KBD prevalence rate in Changdu of Tibet.Methods The aged 7~12 year children are tested with X- ray and epidemiological investigation.Results Xizang are still high yet,the 3 of 4 porints X-rates are more than 20% and the highest one is 55.34%.Conclusions The Changdu is the severest point of KBD in our country.
2.Influences of Radix Astagali seu Hedysari on contents of nitric oxide and malondialdehyde in brain tissue of young rats with cerebral injury after cerebral ischemia and anoxia
Shaodan ZHANG ; Lin PEI ; Chunhua DING ; Yiling LING
Chinese Journal of Tissue Engineering Research 2005;9(37):185-187
BACKGROUND:After cerebral tissue ischemia and anoxia in young rats,the cerebral edema gets serious, and the levels of nitric oxide (NO) and malondialdehyde (MDA) decrease. Radix Astagali seu Hedysari has the pharmacological effects of enhancing immunity, anti-anoxia and improving myocardial ischemic reinfusion injury.OBJECTIVE: To investigate the influences of Radix Astagali seu Hedysari (huangqi) on contents of NO and MDA in brain tissue of young rats with cerebral injury after cerebral ischemia and anoxia.DESIGN:A randomized and controlled trial.SETTING: Department of Pediatrics, Second Hospital, Hebei Medical University; Department of Internal Medicine, Institute of Traditional Chinese Medicine, Hebei Medical University; Department of Pathophysiology, Hebei Medical UniversityMATERIALS:The experiment was conducted from January to April 2004at Department of Pathophysiology, Hebei Medical University. Total 40 SD rats, 7-day old, were at random divided as normal control group, model group, humgqi low-dose group and huangqi high-dose group, with 10 rats in each group. Huangqi injection (The content in 10 mL injection is consistent with 20 g raw drug) was provided by Institute of Traditional Chinese Medicine of Hebei Medical University (produced in Chengdu Di'ou Jiuhong Pharmaceutical Factory, Batch No. 0005028).METHODS:Except rats in normal group, those in the rest groups, under conscious and local anesthesia, were all given common carotid artery ligation, establishing cerebral injury model due to ischemia and anoxia. Rats in normal group were intraperitoneally injected 0.1 mL normal saline; rats in model group were intraperitoneally injected 9 g/L normal saline, 0.1 mL each day; rats in huangqi low-dose group and huangqi high-dose group were respectively given 0.1mL, 0.5 mL huangqi injection, once a day, intraperitoneally. Cerebral blood flow was detected immediately, 2 and 4days after injection. Then the rats were decapitated for collecting the brains to measure the water content in brain, the contents of NO and MDA.MAIN OUTCOME MEASURES: [1] Water contents in brains of rats in every group. [2] Cerebral blood flow, and the contents of NO and MDA.RESULTS:Totally 40 rats were involved in the trial and all entered in the final result analysis. [1] The water content in brain of each group: Compared with normal group, the content in model group was increased immediately after model establishment [(87.316±0.275)%, (88.259±0.297)% ,P < 0.05 ],and did not return to the normal level at the second day [(86.973±0.265)%,(88.173±0.445)%,P < 0.05]; compared with model group, the content in huangqi high-dose group was obviously decreased at second day[(88.173±0.445)%, (86.542±0.141)% ,P < 0.05]. [2] Measurement of cerebral blood flow: compared with control group, the blood flow in model group was obviously decreased immediately after model establishment[(231.88±13.33), (139.54±10.58)mV,P< 0.05], and did not return to normal level till the 4th day [(234.57±14.38), (145.38±13.33)mV,P < 0.05];compared with model group, the blood flow in huangqi low-dose group and huangqi high-dose group, at day 4, was obviously increased [(145.38±13.33),(288.45±12.89), (313.82±21.74)mV,P < 0.01]. [3] The contents of NO and MDA: The contents in model group, immediately after model establishment, were obviously higher than those in normal control group [(26.55±5.23 ), ( 19.67±7.17 )μmol/L,P < 0.05; (7.88±2.55), (4.22±0.12) μmol/L, P< 0.01], and at day 4, were significantly higher than those in normal control group [(48.65±17.06), (18.65±2.12)μmol/L,P < 0.01; (5.29±0.68),(4.06±0.39)μmol/L,P < 0.05]; compared with model group, the contents in huangqi low-dose group and huangqi high-dose group were obviously decreased at day 4 [(48.65±17.06), (23.77±12.79), (24.67±11.54)μ mol/L,P< 0.01; (5.29±0.68), (4.51±2.30), (3.68±0.39)μmol/L,P < 0.01].CONCLUSION:Huangqi could obviously reduce cerebral edema from ischemia and anoxia, increase cerebral blood flow. It could decrease the contents of NO and MDA that is metabolite of free radical injury, thus playing its role to inhibit lipid peroxidation injury.
3.Relationship between plasma adiponectin and TNF-? concentration in women with gestational diabetes
Zengjuan LIU ; Fengmin PEI ; Yan LI ; Jinyun DING ; Feng ZHANG
Basic & Clinical Medicine 2006;0(10):-
Objective To investigate the serum adiponectin levels and TNF-? levels in women with gestational diabetes and to study their clinical significance.Methods Plasma concentration of adiponectin,tumor necrosis factor alpha and correlate parameters were measured in 50 patients with gestational diabetes(GDM)and 36 pregnant women with normal glucose tolerance(NGT).Radio immunoassay was used to measure the adiponectin,TNF-? level was measured by ELISA.Serum insulin level was measured by Electrochemiluminescent immunoassays fasting glucose levels by glucose oxidase method.Results GDM patients had significantly lower concentrations of adiponectin(10.3?(2.4) mg/L) and elevated levels of TNF-?(4.6?(1.5)?g/L) in comparison to NGT women.In GDM group TNF-? level was correlated positively with serum insulin level,glucose level.Concentration of adiponectin was negatively correlated with corresponding parameters.Conclusion Elevated level of TNF-? and decreased adiponectin concentration may not simply reflect maternal adiposity and insulin resistant state,but may contribute to the impaired glucose metabolism during pregnancy and forecast the risk of metabolism syndrome.
6.Sampling results and quality assessment of recombinant human interferon α1b injection
Dening PEI ; Youxue DING ; Ying GUO ; Xinchang SHI ; Hua BI ; Xi QIN ; Chunming RAO
Drug Evaluation Research 2017;40(3):341-344
Objective To evaluate the quality status of recombinant human interferon α1b injection and find out some quality problems.Methods Totally 31 batches of recombinant human interferon α1b for injection and 11 batches of recombinant human interferon α1b injection from two enterprises were examined according to Chinese Pharmacopoeia Volume Ⅲ (2010),and the quality status of recombinant human interferon α1b injection was evaluated by statistical analysis of the results.Results All 42 batches of samples were qualified.The production process of each enterprise was steady.Conclusion At present the quality of recombinant human interferon αlb injection is generally good.The current standards are feasible,but the specified standard of osmolality needs to be improved.
7.Association of vitamin D receptor gene polymorphisms and haplotypes with Crohn ' s disease
Jihua PEI ; Hao WU ; Xiuqing LIN ; Jie JIN ; Ran DING ; Yi JIANG
The Journal of Practical Medicine 2017;33(5):735-738
Objective To analyze the association of Crohn's disease(CD)with vitamin D receptor(VDR) gene polymorphisms. Methods After collecting 326 CD patients and 464 healthy controls,the four single nucleotide polymorphisms of VDR (FokI, BsmI, ApaI and TaqI) were examined by a SNaPshot technique. Results Compared with those in controls,the frequencies of mutant allele(A)and genotype(GA+AA)of BsmI were significantly decreased in CD patients(both P=0.001). The similar conclusions were also drawn for the mutant allele(C)and genotype(TC+CC)of TaqI(both P<0.05). In further stratified analysis,compared with those in controls,the mutant alleles and genotypes of BsmI and TaqI were significantly reduced in stenotic type CD patients (all P<0.0083). The analyses of linkage disequilibrium(LD)and haplotype showed that BsmI,ApaI and TaqI were in a strong LD,and the formed haplotype AAC was significantly lower in CD patients than that in controls (P <0.05). Conclusions VDR(BsmI and TaqI)polymorphisms are significantly related with the reduced susceptibility to CD,especially for patients with stenotic CD. Moreover,the haplotype AAC might engender a reduced risk of CD.
8.EFFECT OF CHINESE WILD RICE ON LIPID METABOLISM AND INFLAMMATORY FACTORS IN RATS FED WITH HIGH CHOLESTEROL DIETS
Hong ZHANG ; Pei CAO ; Chengkai ZHAI ; Zhoubo DING ; Yanbo GUO ; Qun ZHANG
Acta Nutrimenta Sinica 1956;0(03):-
Objective To study the effect of Chinese wild rice diet on lipid metabolism in rats. Method Forty four male SD rats were divided into 4 groups:control group, high lipid group, white rice-flour group and Chinese wild rice group. All groups were given different experimental diets for 8 w and body weights, serum TC, TG, HDL-C, high sensitive C-reactive protein (hs-CRP), TNF-? and IL-6 were measured. Results The hyperlipidemic rat model was successfully induced. When compared with high lipid group and white rice-flour diet group, serum TG and TC contents were significantly decreased, and HDL-C significantly increased in the Chinese wild rice group. Moreover, Chinese wild rice group had lower contents of serum hs-CRP and TNF-? than those in high lipid group and white rice-flour group, but no effect on serum IL-6. Conclusion Chinese wild rice could improve lipid metabolism and low-grade inflammation of hyperlipidemic rats induced by high lipid diet.
9.Sampling Results and Quality Assessment of Recombinant Human Interferon α2 a Injections
Dening PEI ; Ying GUO ; Yonghong LI ; Chunmei HAN ; Youxue DING ; Xiang LI
China Pharmacist 2015;(1):52-54,55
Objective: To evaluate the quality status of recombinant human interferon α2a injections and find out some quality problems. Methods:The statutory testing methods combining with the exploratory studies were used to examine the samples, and the quality status of recombinant human interferon α2a injections was evaluated by statistical analysis of the results. Results: All 28 bat-ches of the injections were qualified using the statutory testing methods. The exploratory studies showed that if the specific activity was determined, the qualified rate was only 87. 0%. All 7 batches of drug substances were qualified using the statutory testing methods. The exploratory studies showed that if the related protein was determined, the qualified rate was 57. 1%. Conclusion:At present the quality of recombinant human interferonα2a injections is generally good. The current standards are feasible;however, improvement is still needed. Specific activity determination should be supplemented the standards for drug products and related protein determination should be supplemented the standards of drug substances.
10.QT dispersion in acute pulmonary embolism.
Xiaoxue DING ; Saidan ZHANG ; Zhifang PEI
Journal of Central South University(Medical Sciences) 2013;38(4):395-399
OBJECTIVE:
To explore the alteration and the clinical significance of QT dispersion in acute pulmonary embolism (PE).
METHODS:
From May 2011 to April 2012, 42 hospitalized PE patients in Xiangya Hospital of Central South University were enrolled, and divided into a high-risk group and a non-high-risk group according to the clinic state on admission. Another 30 healthy subjects with matched age and genders were enrolled as a normal control group. QT interval was measured manually in 12- lead conventional electrocardiogram within 24 hours on admission and after the treatment. QT dispersion (QTd) and heart rate-corrected QT dispersion (QTcd) were also calculated. All patients were followed up during hospitalization, and were divided to a death group and a survival group.
RESULTS:
QTd and QTcd in the high-risk group [(70.2±34.0), (88.1±43.3) ms] and the non-high-risk group [(49.3±21.8), (59.1±26.2) ms] were significantly higher than those in the normal control group[(33.2±12.4), (36.7±14.2) ms] (P<0.05), while QTd and QTcd in the high-risk group were significantly higher than those in the non-high-risk group (P<0.05). The interval of electrocardiogram was (5.6±2.5) days between 24 hours on admission and after the treatment (ECG). QTd and QTcd were reduced significantly after the treatment in the survival group [(41.0±16.4), (47.4±18.0)ms] compared with those on admission [(54.0±33.0), (67.2±40.5)ms] (P<0.05), but the QTd and QTcd after the treatment were also significantly higher than those in the normal control group (P<0.05). There was no significant difference in the QTd and QTcd between 24 hours on admission and after the treatment in the death group (P>0.05). Logistic regression showed that high-risk of PE, right ventricular dysfunction and high QTcd after the treatment were the main risk factors of hospital death.
CONCLUSION
QTd and QTcd are increased in PE. PE patients with right ventricular dysfunction, high-risk of PE, and high QTcd after the treatment suggest weak prognosis.
Adolescent
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Adult
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Aged
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Case-Control Studies
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Electrocardiography
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Female
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Heart Conduction System
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physiopathology
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Humans
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Male
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Middle Aged
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Prognosis
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Pulmonary Embolism
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complications
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physiopathology
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Ventricular Dysfunction, Right
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etiology
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physiopathology
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Young Adult