Objective: To study clinical significance of endogenous vitamin C 、 E in patients With hypertension and stroke. Methods: The serum vitamin C、 E, Malondialdehyde (MDA), superoxide dismutase (SOD) were determined by ultraviolet spectrophotometry in patients with hypertension (23 cases)、 hypertensive cerebral hemorrhage (CH, 30 cases)、 ischemic cerebrovascular disorders (IC, 25 cases) and control groups (20 cases). Results: Compared with control group, at acute stage of stroke, the Vit C、 E and SOD levels significantly reduced (P＜0. 01), the MDA level significantly increased (P＜0.01); at convalescent stage, the Vit C、 E gradually recover to normal, the SOD still reduced (P＜0.05), the MDA still incresed (P＜0.05). Conclusion: The endogenous Vit C、 E have significant change in patients with acute stage of stroke, it gradually recovers to normal in convalescent stage. Meanwhile the SOD and MDA change accordingly.

Objective To investigate the relationship between CD+3CD28+T cell value and chemotherapeutic efficacy in patients with advanced digestive cancer. Methods 38 digestive cancer patients with distant metastatic lesion were included.10 healthy persons were as control group. The absolute counts of CD+3CD28+T cell were measured with flow eytometry in these patients before chemotherapy. All patients received chemotherapy, gastric cancer: DCF regimen (docetaxel plus tegafur and cisplatin, one cycle), colorectal cancer:. Folfox regimen (L-OHP plus tegafur, two cycles), esophageal cancer: mitomycin plus tegafur and cisplatin (two cycles), gallbladder or pancreatic cancer: gemzar plus tegafur and cisplatin (one cycle). According to WHO standard, the chemotherapeutic efficacy was determined, 38 patients were divided into three groups: CR+PR (complete remission plus partial remission), SD (stable of disease) and PD (progress of disease) group. Statistical analysis was used upon all data. Results There was no significant difference in value of CD+3CD28+ T cell between CR+PR group and control group (P=0.549). The value of CD+3CD28+ T cell in SD group and PD group were significantly decreased (P=0.002, P=0.000). The value of CD+3CD28+T cell in CR+PR group was more than that in PD group (P =0.001), while in SD group was more than in PD group (P = 0.044). Conclusion The efficacy is poor, if the value of CD+3CD28+ T cell in patients with advanced digestive cancer is less than that of in healthy persons in peripheral blood. Therefore, the value of CD+3CD28+ T cell is important for the early prediction of the efficacy before chemotherapy.

concern in breast cancer involves changes in weight which may adversely affect the prognosis of patients. This study intended to evaluate weight changed uring chemotherapy and its association with prognostic outcome measured as event-free survival (EFS)among breast cancer patients. A total of63 women diagnosed with breast cancer and have been treated by anthracycline-based chemotherapy between 2005 and 2011were included.A weight change (WC) was calculated based on relative percentage of weight changes from baseline to post-chemotherapy and categorized into either weight change (WC >5%) or stable (±5% WC).Survival probabilities were estimated using the Kaplan-Meier (SPSS 20). Upon treatment completion, 42.9% of the women experienced weight changes. A small weight reduction was observed (61.1 ± 11.6kg to 60.2 ± 11.9kg; -0.8 ± 4.0kg; p=0.106). The median EFS of women who experienced weight change was shorter (median 61.0 months) compared to those who maintained their weight (median 89.0 months) (p=0.044). In this study, weight changes during breast cancer chemotherapy were associated with poorer prognosis in comparison to women who maintained their weight.

Objective To investigate the application effect of quality control management in the prevention of indwelling catheter associated urinary tract infection in intensive care unit (ICU) patients. Methods Cases of patients stayed in the department of ICU undergoing indwelling catheter over 10 d were selected by using time stage sampling method. Totally 136 cases of patients were selected from January 1, 2012 to December 31, 2012, as the control group. A total of 145 cases of patients were selected from January 1, 2013 to December 31, 2013, as the performance group. Cases in performance group were taken standardized training and quantify the performance appraisal on the basis of measures in the control group. The urine routine was tested and bacterial was cultured at indwelling catheter 3 d, 7 d and 10 d, respectively. The indwelling catheter associated urinary tract infections of the two groups were compared. Results After the implementation of the performance appraisal management, the incidences of indwelling catheter associated urinary tract infection at 3 d, 7 d and 10 d were 4.8%(7/145), 19.3% (28/145) and 32.4% (47/145), respectively. Within each quarter, the incidence of indwelling catheter associated urinary tract infection was rising with indwelling catheter time prolonged. And the incidences of the first and second quarter were higher than three and four quarter. The incidences of indwelling catheter associated urinary tract infections at 3 d, 7 d and 10 d in the performance group were lower than the control group, the differences were statistically significant (χ2=4.494, 30.660 and 49.307, P < 0.05). Conclusions Standardized training of nursing staff in ICU and implementation of performance appraisal management could effectively improve the enthusiasm and sense of responsibility of the nurses, and effectively reduce the incidence of indwelling catheter associated urinary tract infection.

Objectives: This paper mainly intended to review the experiences and challenges encountered particularly in mental health issues and to additionally analyze the methodologies used in studies involving HIV/AIDS informal caregivers. Methods: Four electronic databases; Science Direct, EBSCOhost, Ovid and Springer Link were searched for articles published in the past 10 years (2002 – 2012). Only full-text English articles related to research on care giving of HIV-infected adult patients were selected. Results: Twenty two out of 293 articles (7.5%) were reviewed, involving 2,765 caregivers in the USA (n=1,610), Africa (n=253), Asia (n=838) and Oceania (n=64) regions. A variety of age categories was involved in care giving with the youngest carer being 12 years old and the oldest, 60 years on average. Females and whites appeared to be dominant and 603 caregivers themselves were HIVpositive. The main outcomes measured were care giving burden, challenges and coping. Stress and depression, stigma and discrimination, insufficient support, role overload and extreme poverty were the main challenges experienced in care giving. Both qualitative (n=11) and quantitative (n=9) were the equally preferred types of study. Purposive sampling emerged as the most preferred sampling technique. Various instruments were utilized, but the Beck Depression Inventory (BDI) was the most popular particularly in quantitative studies. Conclusion: A variety of life aspects were negatively affected in the process of care giving for HIV/AIDS patients and studies of such nature commonly focused on caregivers’ psychosocial burden.

This research is to investigate study the flavonoids from stems of Nelumbo nucifera and the cytotoxic activities of iso- lated compounds. The constituents were separated by column chromatography,and their structures were elucidated by spectroscopic data analyses. The isolated compounds were evaluated for cytoxic activities by MTT method. Twelve compounds were isolated and identified as rhamnazin-3-O-beta-D-glucopyranoside (1), luteolin-3', 4'-dimethylether-7-O-beta-D-glucoside (2), kaempferol-3-O-beta-D-xylopyranosyl-(1-->2)-O-beta-D-glucopyranoside (3), quercetin-3,3'-di-O-beta-D-glucopyranoside (4), 1, 8-dihydroxy-3,7-dimethoxyxanthone (5), isorhamnetin-3-O-beta-D-glucopyranoside(6) , kaempferol(7), isorhamnetin (8), quercetin(9), astragalin(10), hyperoside (11) and 1-hy- droxy-3,7,8-trimethoxyxanthone(12). All compounds were isolated from stems of this plant for the first time, and compounds 1-5 were firstly isolated from the family nelumbonaceae. Compounds 24 and 6 showed significant cytotoxic activities against BEL-7402 carcinoma cell lines at a concentration of 1 x 10(-5) mol x L(-1) with the inhibitory rate of 67.36%, 53.25%, 57.78%, 60.13% and 52.11%, respectively.
Cell Line, Tumor ; Flavonoids ; chemistry ; pharmacology ; Humans ; Nelumbo ; chemistry ; Plant Extracts ; chemistry ; pharmacology ; Plant Stems ; chemistry

Drugs, Chinese Herbal ; administration & dosage ; therapeutic use ; Humans ; Phytotherapy ; methods ; Pulmonary Disease, Chronic Obstructive ; drug therapy

Aged ; Anion Exchange Protein 1, Erythrocyte ; metabolism ; Brenner Tumor ; metabolism ; pathology ; surgery ; Carcinoma, Transitional Cell ; metabolism ; pathology ; surgery ; Chromogranin A ; metabolism ; Cystectomy ; Female ; Humans ; Hysterectomy ; Membrane Proteins ; metabolism ; Neoplasms, Multiple Primary ; metabolism ; pathology ; surgery ; Ovarian Neoplasms ; metabolism ; pathology ; surgery ; Urinary Bladder Neoplasms ; metabolism ; pathology ; surgery

OBJECTIVE: To establish an HPLC method for the simultaneous determination of butorphanol tartrate, dextroisomer and benzethonium chloride which works as the bacteriostatic agent in butorphanol tartrate injection on a cyclodextrin chiral column. METHODS: The HPLC analysis was performed on an Astec Cyclobond II cyclodextrin chiral column (4.6 mm×250 mm, 5 μm), with mobile phase consisting of 0.05 mol·L-1 ammonium acetate solution (pH was adjusted to 4.1 by acetic acid) and acetonitrile using gradient elution at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 280 nm. RESULTS: The method showed good linearity for the three components to be measured. The linear ranges were 0.04-2.0 mg·mL-1 for butorphanol tartrate (r=0.999 9), 0.01-2.0 mg·mL-1 for dextroisomer (r=1.000 0), and 0.02-1.0 mg·mL-1 for benzethonium chloride (r=0.999 9), respectively. The average recoveries were 100.2%, 100.7%, and 99.4%, respectively. CONCLUSION: The method is simple, accurate and reproducible. It can be used for the quality control of butorphanol tartrate injection.

OBJECTIVE: To study the absorption and metabolism of active compounds of Polygoni Multiflori Radix in L-02 cells and elucidate their chemical structures by liquid chromatography tandem-mass spectrometry. METHODS: The active compounds in Polygoni Multiflori Radix, 2,3,5,4'-tetrahydroxystilbene-2-O-γ-5-glucoside (TSG) and emodin (EN), were cultured into L-02 cells. Cell culture media and cell lysis were collected after 0,2,4,6,8,12,24,36 and 48 h respectively. Reversed-phase high-performance liquid chromatography (HPLC) was used to explore the possible absorption and metabolism. Liquid chromatography-tandem-mass spectrometry (LC-MS/MS) was used to identify the chemical structures of the metabolic products. RESULTS: TSG showed no obvious accumulation in L-02 cells. One metabolic product of TSG was detected by HPLC, and its concentration increased with lime after 8 h. This metabolite was identified as the glucuronidation of TSG by LC-MS/MS. In contrast, EN was partially accumulated in L-02 cells. Three isomers of glucuronidations and one sulfation of EN were also identified by LC-MS/MS. CONCLUSION: EN is easier to be absorbed and accumulated in L-02 cells than TSG. Phase II metabolic reactions play an important role in the metabolism of TSG and EN in cells. However, the accurate binding sites need to be further confirmed.