Adult ; Fracture Fixation, Internal ; Fractures, Bone ; complications ; surgery ; Humans ; Joint Dislocations ; surgery ; Male ; Middle Aged ; Talus ; injuries ; surgery ; Treatment Outcome ; Young Adult

BACKGROUNDAnatomic and electrophysiological studies have revealed that the neurons located in the media vestibular nuclei (MVN) receive most of the sensory vestibular input coming from the ipsilateral labyrinth and the responses of MVN neurons to caloric stimulation directly reflect changes in primary vestibular afferent activity. The aim of this study was to clarify the intrinsic characteristics of serotonin (5-hydroxytryptamine, 5-HT) release in the MVN during the period of vertigo induced by caloric stimulation.

METHODSWe used an in vivo microdialysis technique to examine the effects of caloric stimulation on the serotoninergic system in MVN. Twenty four guinea pigs were randomly divided into the groups of irrigation of the ear canal with hot water (n = 6), ice water (n = 6) and 37 degrees C water (n = 4), and the groups of irrigation of the auricle with hot water (n = 4) and ice water (n = 4), according to different caloric vestibular stimulation. We examined the animal's caloric nystagmus with a two-channel electronystagmographic recorder (ENG), and meanwhile examine serotonin (5-hydroxytryptamine, 5-HT) level in the MVN with microdialysis technique after caloric stimulation.

RESULTSIn the caloric test the hot water (44 degrees C) irrigation of the right external auditory canal induced horizontal nystagmus towards the right side lasting about 60 seconds and the ice water irrigation of the right external auditory canal induced it towards the left side lasting for about 90 seconds. No nystagmus was induced by 37 degrees C water irrigation of the external ear canal. Therefore, it was used as a negative control stimulation to the middle ear. The MVN 5-HT levels significantly increased in the first 5-minute collecting interval and increased to 254% and 189% of the control group in the second collecting interval in response to caloric vestibular stimulation with ice water and hot water respectively. The serotonin release was not distinctly changed by the irrigation of the auricle with ice water or hot water.

CONCLUSIONSNeither somato-sensory stimulation of the middle ear nor nonspecific cold or hot stress affects the serotonin release. The rise of 5-HT in MVN may be involved in the mechanism of vertigo induced by caloric stimulation.

Animals ; Caloric Tests ; Guinea Pigs ; Microdialysis ; Serotonin ; secretion ; Vertigo ; etiology ; Vestibular Nuclei ; pathology

OBJECTIVEObserving the dynamic change characteristics of serum liver function indexes in occupational dermatitis medicamentosa-like of trichloroethylene patients with liver damage, we can underlie for guiding therapy, prognosis and mechanism of dermatitis medicamentosa-like of trichloroethylene patients with liver damage.

METHODSWe collected serum of 10 cases of occupational dermatitis medicamentosa-like of trichloro-ethylene patients with liver damage from different time points since they were hospitalized, using automatic biochemistry analyzer to detect total protein (TP), albumin (ALB), total bilirubin (TBIL), direct bilirubin (DBIL), indirect bilirubin (IBIL), alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transpeptidase (GGT), alkaline phosphatase (ALP), albumin/globulin ratio etc 11 liver function biochemical indicators. We used Excel to establish database, professional drawing software gnuplot to draw dynamic variation diagram of each index.

RESULTSThe variation range of 11 liver function indexes of 10 cases was TP 43.2-74.2 g/L, ALB 24.6-44.6 g/L, A/G 0.77-2.10, TBIL 3.7-268.2 umol/L, DBIL 1.0-166.0 umol/L, IBIL 2.4 -167.5 umol/L, ALT 11-5985 U/L, AST 14-5586 U/L, GGT 15-1500 U/L, ALP 35-309 U/L, S/L 0.07-1.94, respectively. TBIL, DBIL, ALT, AST, GGT, ALP concentration significantly increased, especially ALT, AST, GGT, ALT topped 5985 U/L, AST topped 5586 U/L, GGT topped 1500 U/L. But TP, ALB and S/L significantly decreased, TP lowest to 43.2 g/L, S/L lowest to 0.07. A/G basically remained unchanged, but IBIL didn't change regularly.

CONCLUSIONThe early liver damage in dermatitis medicamentosa-like of trichloroethylene patients was serious, and repeatedly attacked, so we should lead to enough attention to the clinical work and prevention. This also provided the basis for studying the mechanism of trichloroethylene poisoning.

Adolescent ; Adult ; Bilirubin ; blood ; Dermatitis, Occupational ; blood ; physiopathology ; Female ; Humans ; Liver ; enzymology ; physiopathology ; Liver Function Tests ; Male ; Trichloroethylene ; Young Adult

Objective To analyze and compare the MRI appearances of ovarian thecoma with pathologic findings in order to improve the knowledge of the disease.Methods Nineteen cases of ovarian thecoma confirmed by histopathology were analyzed.MRI morphological characteristics and signal intensity of the lesions were observed and compared with findings of pathomorphology.Correlation analysis between tumor size and amount of ascites was made.Results Ovarian thecoma displayed iso-or hypointense signal on T_1WI and significant hypointensity in the focal lesion on T_2WI.Hyperintensity occurred when cystic degeneration of the lesions existed.Fibrous septation was detected in some lesions.After enhancement,most lesions showed mild early enhancement with slight increase on the delayed phase.Pathological necrosis and cystic degeneration were seen in 9 cases which corresponded to the number and shape of the cystic lesions on MRI.A large amount of collagen hyperplastic was found between the oncocytes microscopically in 15 cases, which displayed significant hypointensity in the focal lesion on T_2WI;another 4 cases showed relatively less amounts of collagen hyperplastic and more oncocytes,which appeared moderate intensity in the focal leisom on T_2WI.The amount of ascites was not significantly correlated with the lesion size(r=0.43,P=0.10). Conclusions Hypointensity on T_2WI and mild enhancement pattern due to poor blood supply are the characteristics of ovarian thecoma.The MR findings can reflect the pathologic features of the tumor,which is helpful in the diagnosis and differential diagnosis.

Diacylglycerol, DAG, because of its multifunctional and nutritional properties, attracted considerable attention recently. Enzymatic synthesis of diacylglycerols from linoleic acid was investigated in a solvent-free reaction in a continuously operated fixed bed reactors containing Lipozyme RM IM. By appropriate manipulation of the fluid-residence time, the relative proportions of the various acylglycerols in the effluent stream can be controlled. In addition, the presence of excess glycerol is effective for the removal of water produced during the esterification reactions. Under the conditions of molar ratio of linoleic acid to glycerol of 0.5, the immoblized enzyme maintained high stability and allowed the reaction to continue for 10 days without significant deterioration in enzyme activity. It was determined that the conversion of fatty acid, content of 1,3-DAG and volume efficiency of reactor reached optima under the conditions: a packaged-bed reactor(with a ratio of packed length to inner diameter of 7.8), reacting temperature at 65 degrees C, molar ratio of linoleic acid to glycerol of 0.5, and feeding flow rate of 1.2 mL/min.
Catalysis ; Diglycerides ; chemical synthesis ; Enzyme Stability ; Enzymes, Immobilized ; chemistry ; Lipase ; chemistry

Objective To evaluate the efficacy and safety of liraglutide ( Lira) combined with insulin aspart 30 injection in the treatment of type 2 diabetes poorly controlled with premixed insulin aspart 30 ( Asp 30 ) . Methods Seventy eight patients with type 2 diabetes mellitus without reaching glycemic targets were randomized into two groups.On the basis of the insulin aspart 30 and oral hypoglycemic drugs, the patients in the trial group were given liraglutide 0.6 mg? d-1 sc once per night for the first week, and 1.2 mg? d-1 for the following two to sixteen weeks, while the other patients in the control group received insulin detemir ( Det) sc once a night for 16 weeks, with the beginning dosage of 0.2 U? kg -1? d-1.Before and after the treatment, the body mass index ( BMI) , waist to hip ratio ( WHR) , fasting plasma glucose ( FPG) , fast-ing C -peptide ( FCP ) , 2 -hour postprandial plasma glucose (PPG 2 h), glycosylated hemoglobin A1c (HbA1c) and insulin resist-ance index(HOMA-IR) were tested.Also, the adverse drug reactions such as weight gain and hypoglycemia reaction were estimated. Results After the treatment, the level of FPG, PPG 2 h and HbA1c decreased obviously in both groups ( P<0.01 ) , while PPG 2 h in the trial group appeared a better improvement compared to the control group ( P<0.05) ,and BMI as well as HOMA-IR was improved significantly in the trial group after the treatment ( P<0.01).Both drugs were well tolerated with minor hypoglycemia, yet Lira appeared much safer than Det.Conclusion For treatment of refractory type 2 diabetes mellitus, liraglutide combined with insulin aspart 30 injection had certain clincal efficacy with minor adverse reaction.

OBJECTIVE To observe both TRPA1 and TRPM8 expressions in synovial tissues of rats with cold hyperalgesia induced by experimental knee osteoarthritis(KOA).METHODS 45 Sprague Dawley male rats,weight ranging from 440 g to 470 g were randomly individed into the model group,model-antagonist and normal group.Paw withdrawal latency (PWL) of five rats from each group was determined one week before modeling,2,4,6 and 8 weeks after modeling,respectively.8 weeks after modeling,the rats were killed and synovial and cartilage tissues from diseased knee were collected,to observe pathological morphology at cartilage tissues and to determine the protein and gene expressions of TRPA1 and TRPM8 at syno-vial tissues.RESULTS KOA rats showed obvious cold hyperalgesia from two weeks after modeling to the latest follow-up,8 weeks after modeling.The abnormally low level of PWL could be increased by TRPA1 and TRPM8 ion channel blocker.CON-CLUSION Up-regulating expressions of TRPA1 and TRPM8 participate in the occurrence mechanism of KOA cold hyperalge-sia.

Alkaline Phosphatase ; biosynthesis ; Animals ; Bone Morphogenetic Protein 4 ; Bone Morphogenetic Proteins ; pharmacology ; Cell Line ; Enzyme Activation ; Enzyme Induction ; drug effects ; Imidazoles ; pharmacology ; MAP Kinase Signaling System ; Mice ; Osteoblasts ; enzymology ; Pyridines ; pharmacology ; Smad Proteins ; genetics ; Stem Cells ; enzymology ; p38 Mitogen-Activated Protein Kinases ; physiology

OBJECTIVETo study whether the substitutions at the major hydrophilic region II (MHRII) of hepatitis B surface antigen (HBsAg) will impair the antigenicity of HBsAg.

METHODSFour recombinant plasmids expressing mutant HBsAg (mtHBsAg) P1120T, C121S, K122I and T123N were constructed. HepG2 cells were transfected with the four plasmids and a plasmid expressing G145R HBsAg. The immunoreactivity of the cells expressing mtHBsAg with P1120T, C121S, K122I, T123N and G145R were detected by immunofluorescence (IF) staining and ELISA with 4 antibodies and 7 HBsAg diagnostic kits respectively.

RESULTSmtHBsAg with P120T was recognized by mAb1 and mAb2. mtHBsAg with C121S and K122I was not recognized by any mAbs. mtHBsAg with T123N in lysates was recognized by mAb2, but not recognized in the supernatants.

CONCLUSIONSubstitutions at amino acid positions 120-123 of HBsAg strongly impaired the antigenicity of HBsAg, a fact that was not appreciated previously.

Amino Acid Substitution ; Antigenic Variation ; Hep G2 Cells ; Hepatitis B Surface Antigens ; genetics ; immunology ; Hepatitis B virus ; genetics ; immunology ; Humans ; Mutation ; Plasmids ; Transfection

OBJECTIVETo study the effect of high-density lipoprotein (HDL) on the proliferation of endothelial progenitor cells (EPC) isolated from human umbilical cord blood; to further explore its effect on prevention and development of atherogenesis.

METHODSEPC isolated by density gradient centrifugation were cultured in a M200 medium. Immunofluorescence staining for CD133, CD34, KDR and Factor VIII were adopted respectively as the specific markers for identification. The effect of HDL on EPC proliferation was estimated on the 7th day of cell cultivation using MTT assay, confocal microscopy and fluorescence activated cell sorting.

RESULTSHDL, when incubated with EPC, was able to promote remarkably the proliferation rate of EPC, dose- and time-dependent. HDL participated in the transcriptional regulation of cell cycle by affecting the regulatory proteins such as cyclin D1.

CONCLUSIONSA subtype of progenitor cells was isolated from human cord blood with a potential of differentiating into mature endothelial cells (known as endothelial progenitor cells). HDL plays an important role on EPC fluorescence activated cell sorting differentiation and proliferation. Further studies are required to identify the signal pathway and the molecular mechanism of HDL effect on EPC proliferation.

AC133 Antigen ; Adult ; Antigens, CD ; metabolism ; Antigens, CD34 ; metabolism ; Cell Cycle ; drug effects ; Cell Differentiation ; drug effects ; Cell Proliferation ; drug effects ; Cell Separation ; Cells, Cultured ; Cyclin D1 ; metabolism ; Dose-Response Relationship, Drug ; Endothelial Cells ; cytology ; drug effects ; metabolism ; Factor VIII ; metabolism ; Fetal Blood ; cytology ; Flow Cytometry ; Fluorescent Antibody Technique ; Glycoproteins ; metabolism ; Humans ; Lipoproteins, HDL ; blood ; pharmacology ; Microscopy, Confocal ; Peptides ; metabolism ; Stem Cells ; cytology ; drug effects ; metabolism