Objective To evaluate the effects of rigorous surveillance and retroperitoneal lymph node dissection (RPLND) in the treatment of low-risk patients with clinical stage Ⅰ nonseminomatous germ cell testicular tumors (NSGCT) after radical orchiectomy.Methods The data of 71 patients with clinical stage Ⅰ NSGCT were analyzed retrospectively in Hunan Provincial Tumor Hospital,Xiangya Third Hospital of Central South University and Hunan Provincial People's Hospital between Feb,2001 and Apr,2012.Excluding lymphatic and vascular invasion,percentage of embryonal carcinoma＞50％ and increasing tumour markers (AFP/β-HCG) following orchiectomy,46 low-risk patients out of 71 patients with clinical stage Ⅰ NSGCT were selected and divided into rigorous surveillance group (30 cases) and RPLND group (16 cases) according to different therapeutic methods after radical orchiectomy.Univariate analysis was used to confirm variables associated with disease progression,and the disease free survival rates (DFSR) were compared by using Kaplan-Meier analysis.Results Five cases were lost,and 41 cases were followed up for an average of 61 months (range,15-147 months),with 58 months in rigorous surveillance group (range,19-147months) and 65 months in RPLND group (range,15-144 months).The survival rate was 100％ in 2 groups.The DFSR was 89％ (24/27) and 86％ (12/14),respectively,and there was no significant difference between the 2 groups (x2 =0.08,P=0.78).The DFSR was 83％ in patients with small amout of embryonal (percentage of embryonal carcinoma ＜ 50％),and 92％ in patients without embryonal carcinoma,and there was no significant difference between the 2 groups (x2=1.07,P=0.30).Also there was no significant difference between the patients less than 15 years and patients more than 15 years (x2=1.59,P =0.21).Conclusions There is no significant difference in recurrence rate and DFSR between rigorous surveillance group and RPLND group.Low-risk patients with clinical stage Ⅰ NSGCT may achieve satisfactory prognosis with surveillance after radical orchiectomy.

Abstract: Objective　To explore the spatial epidemiological characteristics of severe cases hand, foot and mouth disease (HFMD) in Guangxi, China, from 2014 to 2018, and to provide a basis for identifying the high-risk regions as well as the prevention and control of severe cases of HFMD in Guangxi. Methods　Spatial-temporal scanning analysis, global and local spatial autocorrelation analysis were used to analyze the spatial clustering of HFMD. The trend surface analysis was used to evaluate the spatial distribution trend of HFMD. Results　From 2014 to 2018, the incidence and severe case fatality rates of HFMD were 3.89/100 000 and 4.23%, respectively. Monte Carlo scanning analysis showed that the first cluster region was Cenxi City, the second cluster was mainly concentrated in northwest of Guangxi, and the aggregation time was mainly concentrated in April to May and August to October. The global spatial autocorrelation analysis showed that the severe HFMD was significant clustering distribution, and the Moran's I coefficients of the sever cases, severe morbidity and severe case fatality rate were 0.088, 0.118, 0.197, respectively (P<0.05). Local spatial autocorrelation analysis showed that hotspots of severe HFMD cases were concentrated in the southern Guangxi, mainly in Lingshan County. Anselin local Moran's I clustering and outlier analysis indicated that 5 high-high (H-H) clustering regions for fatality were Lingshan, Pubei, Zhongshan, Zhaoping and Pinggui County. There were 6 high-high (H-H) clustering regions for severe incidence rate, namely Lingshan, Qinnan, Lingyun, Youjiang, Bama Yao Autonomous and Pinggui County, and 1 high-low (H-L) clustering region, Cenxi County. The trend surface analysis showed that the overall number of severe cases of death decreased from east or west to the middle, and increased from north to middle, and then decreased to south. Conclusions　Severe HFMD cases in Guangxi have obvious spatial-temporal clustering, and the hop spots are mainly concentrated in southern Guangxi. The prevention and control of HFMD in areas with high incidence of severe cases should be strengthened to reduce the burden of HFMD cases.

OBJECTIVETo investigate the influence of the vascular endothelial growth factor( VEGF) antibody targeted vascular therapy on the expression of human collagen type I in hyperplastic scar of nude mice.

METHODSThe hyperplastic scar from one female burn patient with 1% TBSA deep-partial thickness burns were implanted into subcutaneous skin of scapular region of 48 nude mice. Three weeks later, the nude mice were divide into large dose (LA) , medium dose (MD) , small dose (SD) and control groups, with 12 mice in each group. The mice in LA,MD and SD groups were injected with 200 microl of 15,10, 5 microg/ml VEGF monoclonal antibody diluted in 0.01 mol/L PBS, respectively in the scar twice a week for 3 weeks, while those in C group were injected with equal amount of 0. 01 mol/L PBS. The area and volume of the scar in each group were calculated and histological changes were observed, and the expression of collagen type I mRNA and its protein in each group were determined 3 days after treatment.

RESULTSThe volume of scar in LA, MD, SD and C groups were (55.3 +/-4.1, 67.9 +/-5.7, 78.9 +/-5.5, 85.0 +7.3) mm(3), respectively. Compared with that in C group, the volume of the scar were significantly decreased in AD and MD groups ( P <0.05). A few number of vessels and fibroblasts were observed in LD, MD groups, with decreased number of collagen fibers arranged in order. Compared with that in C group ,The expression of procollagen type I mRNA and its protein in C group was obviously higher than those in LD and MD groups ( P < 0. 05) , but it was similar to those in SD group.

CONCLUSIONVEGF targeted vascular therapy is beneficial for the inhibition of the angiopoietins of hyperplastic scar, the expression of collagen , and the growth of scar.

Adult ; Animals ; Antibodies, Monoclonal ; therapeutic use ; Cicatrix, Hypertrophic ; metabolism ; therapy ; Collagen Type I ; metabolism ; Disease Models, Animal ; Female ; Gene Expression ; Humans ; Hyperplasia ; metabolism ; therapy ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Neovascularization, Pathologic ; metabolism ; RNA, Messenger ; metabolism ; Vascular Endothelial Growth Factor A ; immunology

Objective To study the dynamic growth of adsorption films on solid surfaces in point contacts with aqueous protein aqueous solutions and investigate the dominating factors.Methods The formation of the adsorption films within a ball-on-disc contact was measured using optical interference technique.The effects of impact loads,impact times and surface properties of the materials on the adsorption of BSA aqueous solution were also studied.Results Under dynamic conditions,the thickness of the adsorption film was much higher than that under static condition.The adsorption film was growing with increasing impact times and finally the stable thickness could be achieved.Small loads and hydrophobic materials were favorable for the growth of the adsorption film.The BSA adsorption film was easy to grow on the steel surface,but on the ceramic surface the adsorption film thickness was low.Conclusions The research about adsorbed lubricating films evolution of protein aqueous solution under impact loads is of clinical significance for treating and preventing of artificial prosthesis.

Objective To anderstand the metagenesis of Oncomelania snails in the mountainous regions so as to control the spread of snails and the epidemics of schistosomiasis.Methods Observation spot was established at a typical snail habitat close to Puge county,Sichuan province from February 2008 to July 2009.Random sampling was applied to determine the place of each frame during the observation.All the snails in each frame were collected and numbers counted in the laboratory,with the number of mating pairs in each frame also observed.Snails being collected were measured for the body indices and the dissection was carried out to identify gender composition.survival status and the number of live snails in each frame counted.Line graphs of the body indices.mating pairs in each observed months,bar graphs of the snail density,proportions of gender together with the maturity of the snails in each month were drawn.Results The number of live snail existed the whole year and its density fluctuated.All the three kinds of body indices showed the same time trend and a dynamic circulation.The young snail existed all year around and arose constantly in proportion from May,becoming the dominant snailin October to replace the adult snails.The young and adult snails also showed a dynamic alternative.The gender composition showed no significant difference during each month.The number of the mating pairs was more on April.May and June annually,when were the snail's main multiplying stage.Conclusion In mountain area.the young snails existed through all the year while adult snails appeared to be dominantin each month except for October.Oncomelania snail showed a circular process of metagenesis which started in May and finished in October.The snail population presented a dynamic equilibrium.It was concluded that ecological studies on Oncomelania snail were extremely relevant,either to optimally apply the existing control measures or to develop alternative measures for snail control,ecologically or biologically.

BACKGROUND: Microwave treatment is a common physical therapy method that can increase the temperature and blood circulation of deep tissues, and is used for improving fracture repair. However, microwave treatment cannot be used if there is surgically implanted metal plate or screw. OBJECTIVE: To observe the dame of microwave treatment to the tissues surrounding the titanium alloy implants. METHODS: Forty-four New Zealand white rabbits were randomized into experimental and control groups. The model of the fracture at the middle of the femur was established in all rabbits, and the rabbits in the experimental group were implanted with titanium alloy internal fixation systems. A 30-day microwave treatment (2 450 MHz, 20 W or 40 W, 20 minutes daily) was applied to the fracture site in all rabbits at 3 days after operation. RESULTS AND CONCLUSION: After 20 W of wave microwave treatment, the temperature of tissues around the implants showed no significant increase or severe heat injury. While, 40 W of wave microwave treatment significantly increased the temperature of tissues around the implants and the tissue was damaged severely. Our results indicate that, the low-dosage microwave treatment may be a promising method in the rehabilitation therapy of fractures with titanium alloy internal fixation.

OBJECTIVETo investigate the regulatory mechanism of the transcription of tumor metastasis suppressor gene TMSG-1.

METHODSLuciferase reporter assay and site-directed mutagenesis were used to analyze the regulatory region of TMSG-1. Electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) were carried out to verify the interaction of KLF6 and Sp1 with the regulatory region of TMSG-1. Co-immunoprecipitation (CoIP) was performed to analyze the interaction between KLF6 and Sp1. TMSG-1 and wt-KLF6 mRNA expressions in cells with different metastatic capacities were quantitated by real-time PCR. Cell invasive capability was determined by Matrigel invasion assay.

RESULTSA 63 bp inducible regulatory region (+59 bp - +123 bp) in exon 1 was identified by luciferase assay using reporter plasmids with a series of TMSG-1 regulatory region deletions. Mutations in KLF6/Sp1 binding sites of this region resulted in a decrease of luciferase activity, while cotransfection with KLF6 or Sp1 expressing plasmids led to a remarkable increase of luciferase activity. EMSA and ChIP demonstrated that KLF6 as well as Sp1 interacted with this region. CoIP also indicated a possible interaction between KLF6 and Sp1 proteins. In the highly metastatic cell sublines, a low level of wild type KLF6 was associated synchronously with a low TMSG-1 level. Prostate carcinoma cells overexpressing KLF6 exhibited a higher TMSG-1 level and a lower invasive capability.

CONCLUSIONSTranscription factor complex of KLF6 and Sp1 may participate in the inducible transcriptional regulation of TMSG-1, and a decreased wild type KLF6 expression is likely associated with a low TMSG-1 level in the highly metastatic cell sublines.

Binding Sites ; genetics ; Cell Line, Tumor ; Electrophoretic Mobility Shift Assay ; Humans ; Immunoprecipitation ; Kruppel-Like Factor 6 ; Kruppel-Like Transcription Factors ; genetics ; metabolism ; Lung Neoplasms ; metabolism ; pathology ; Male ; Membrane Proteins ; genetics ; metabolism ; Mutagenesis, Site-Directed ; Mutation ; Neoplasm Invasiveness ; Prostatic Neoplasms ; metabolism ; pathology ; Proto-Oncogene Proteins ; genetics ; metabolism ; RNA, Messenger ; metabolism ; Sp1 Transcription Factor ; genetics ; metabolism ; Sphingosine N-Acyltransferase ; genetics ; metabolism ; Transcriptional Activation ; Transfection ; Tumor Suppressor Proteins ; genetics ; metabolism

OBJECTIVETo observe the biomechanic properties of sciatic nerve at the suture site in rats following repairing.

METHODSThe right sciatic nerves of 40 white Sprague-Dawley 300-350 gm rats were exposed, cut and then repaired with 10-0 nylon sutures, laced in the epineurium. 0, 1, 3, 6 weeks after operation, the tensile strength of the sciatic nerves were measured, the data analyzed statistically.

RESULTSThe load-elongation curves for both the normal unoperated and operated nerves had the similar shape. The tensile strength of the 0 week was significant difference to 1, 3 and 6 weeks (P < 0.01). No significant difference was found among 1, 3 and 6 weeks.

CONCLUSIONThe tensile strength of the injured nerves are recovered in the first week and resistant in 6 weeks after repairing.

Animals ; Elasticity ; Male ; Rats ; Rats, Sprague-Dawley ; Sciatic Nerve ; injuries ; physiology ; surgery ; Tensile Strength ; Time Factors

The objective of this study was to elucidate the effect of human placenta adherent cells (hPDAC) on expansion of human umbilical cord blood CD34(+) cells in vitro. hPDAC was isolated and characterized in human placenta tissue by using enzyme-digesting method and flow cytometry. A co-culture system was established with hPDAC and cord blood CD34(+) cells. The CD34(+) cells were cultured in different culture systems with different combinations of hPDAC and SCF, IL-3, IL-6 and FL. The number of total nucleated cells, CFC and CD34(+) cells were repeatedly counted in culture for 4 weeks. The results showed that the hPDAC displayed fibroblast-like morphology, and were positive for CD29, CD44, CD166, HLA-ABC and UEA-1, and negative for CD34, CD45 and HLA-DR. Functionally, ex vivo expansion of CD34(+) cells on feeder layer of placental adherent cells was significantly higher than that no feeder layer group. SCF + IL-3 + IL-6 + FL + hPDAC manifested the most potent combination, with the number of total nucleated cells increasing by (126.0 +/- 6.7)-fold, progenitor cells (CFC) (49.8 +/- 1.7)-fold and CD34(+) cells (8.3 +/- 1.65)-fold. It is concluded that placental adherent cells could support hematopoiesis in vitro and work as a suitable feeder layer for cord blood stem/progenitor cell expansion.
Antigens, CD34 ; analysis ; Cell Adhesion ; Cell Separation ; Fetal Blood ; cytology ; Hematopoietic Stem Cells ; cytology ; Humans ; Placenta ; cytology

OBJECTIVETo establish a computer-aided three-dimensional visualization operation simulation system based on Mimics and Unigraphics NX software to provide reliable evidence for accurate preoperative surgical planning.

METHODSThe preoperative CT scans of 5 patients with intertrochanteric fractures were used for three-dimensional reconstruction of intertrochanteric fractures using Mimics software. Three-dimensional reconstruction of the surgical instruments was carried out using the modeling function of Unigraphics NX software, whose assembly function was used to visualize the simulates internal fixations of intertrochanteric fractures with dynamic hip screw (DHS) system. The operative procedures simulated by Unigraphics NX were analyzed preoperatively.

RESULTSThe virtual surgery procedures were clearly and vividly visualized in three dimensions. The fracture reduction and surgical effects could be predicted using this system.

CONCLUSIONSThis system of three-dimensional visualization of virtual surgery for intertrochanteric fractures has important values in surgical planning, risk assessment and clinical training, and can help improve the reliability and outcome of orthopedic surgery.

Bone Screws ; Computer Simulation ; Female ; Hip Fractures ; surgery ; Humans ; Imaging, Three-Dimensional ; Male ; Middle Aged ; Software ; Surgery, Computer-Assisted ; Tomography, X-Ray Computed ; User-Computer Interface