2.Effect of TGF-? on the expression of type Ⅰ inositol 1,4,5-triphosphate receptor in WB rat liver epithelial cells
Chinese Journal of Immunology 2000;0(08):-
Objective:To investigate the effect of TGF-? on the expression of type Ⅰ inositol 1,4,5-triphosphate receptor in WB rat liver epithelial cells.Methods:The expression of type Ⅰ inositol 1,4,5-triphosphate receptor protein and mRNA were detected by Western blot and RT-PCR in WB rat liver epithelial cells in vitro stimulating with TGF-?.Results:The expression level of type Ⅰ inositol 1,4,5-triphosphate receptor protein and mRNA in WB rat liver epithelial cells were both increased after stimulating with TGF-? in several time points and reached the highest at 8 h stimulated, and then decreased.Conclusion:TGF-? enhance the IP_3R1 protein and mRNA expression in WB rat liver epithelial cells.
3.Correlation Study between Electronic Bronchus Mirror and Chinese Medical Syndrome TVDinci of Mycoplasma pneumonia Children.
Xiu-tai YUAN ; Rui-feng LI ; Pei-li YANG
Chinese Journal of Integrated Traditional and Western Medicine 2016;36(2):188-190
OBJECTIVETo explore the correlation between electronic bronchus mirror and Chinese medical syndrome typing of Mycoplasma pneumonia children.
METHODSTotally 198 Mycoplasma pneumonia children inpatients were assigned to three syndrome types according to Chinese medical syndrome typing and self-formulated typing standards of electronic bronchus mirror, i.e., Fei-qi accumulation of damp and heat syndrome, Fei-qi accumulation of toxicity and heat syndrome, deficient vital qi leading to lingering of pathogen syndrome. The correlation between electronic bronchus mirror and Chinese medical syndrome typing was explored.
RESULTSAs for comparison between electronic bronchus mirror and Chinese medical syndrome typing, Kappa value (K^) was 0.645 and Spearman coefficient correlation (r) was 0.653 (P < 0.01) for Fei-qi accumulation of damp and heat syndrome; K^ was 0.724 and r(s) was 0.727 (P < 0.01) for Fei-qi accumulation of toxicity and heat syndrome; K^ was 0.506 and r(s) was 0.515 (P < 0.01) for deficient vital qi leading to lingering of pathogen syndrome.
CONCLUSIONChinese medical syndrome typing of Mycoplasma pneumonia children was moderately in line with inspection typing under electronic bronchoscope with significant correlation.
Bronchoscopy ; Child ; Humans ; Inpatients ; Medicine, Chinese Traditional ; Pneumonia, Mycoplasma ; classification ; diagnosis
4.Analysis of the Effect of Vitapex Paste in the Root Canal for Deciduous Teeth with Chronic Apical Periodontitis
Li GUO ; Xi CHEN ; Pei LI ; Tong LIN ; Ying FENG
Progress in Modern Biomedicine 2017;17(27):5287-5289
Objective:To investigate the effect of Vitapex paste in the root canal for deciduous teeth with chronic apical periodontitis.Methods:200 children with chronic apical periodontitis were selected and divided into two groups.The observation group (108 cases)was given Vitapex paste.The control group (92 cases) was given Zinc oxide iodoform paste.The effect of Vitapex paste in root canal for deciduous teeth with chronic apical periodontitis was evaluated by filling quality after 1 month'treatment,the VAS scores before and after treatment,long-term curative effect were compared between two groups.Results:After 1 month'treatment,the suitable filling rate of observation group was higher than that of the control group(P<0.05).There was no statistical significance in the VAS scores between two groups (P>0.05).After 1 week'and 1 month'treatment,the pain feeling were alleviated in two groups and significantly lighter in the observation group(P<0.05).After 6 months' follow-up,the success rate was 96.3% in the observation group and 87.0% in the control group,the success rate of observation group was higher than that of the control group (P<0.05).Conclusion:The filling process of Vitapex paste was simple and easy,which had a good therapeutic effect on chronic apical periodontitis of deciduous teeth and could relieve the toothache of patients.
5.Clinical Effect of Modified Simiao San Compared and Colchicine in the Treatment of Damp Heat Gout
Lei FENG ; Ruixia PEI ; Xingbo LI ; Xiaoli LI ; Yanfang WANG
Progress in Modern Biomedicine 2017;17(25):4912-4915
Objective:To compare the clinical effect and safety of modified Simiao San and colchicines on the damp heat gout.Methods:120 patients with damp heat gout admitted in our hospital in 2015 were selected and randomly divided into group A,group B and group C with 40 cases in each group.Patients in group A were given modified Simiao San,patients in group B were given colchicine,patients in group C were given modified Simiao San combined with colchicine.Then the clinical effect,scores of symptoms,expression levels of inflammatory factors and other biochemical indexes,adverse reactions and recurrence rate of 3 groups were compared berween three groups.Results:The total effective rate and effectually effective rate of group A were 92.5% and 57.5% respectively,which were 95.0% and 65.0% in group C was,obviously higher than 77.5% and 35.0% of group B (P<0.05).The symptom scores of 3 groups after treatment were all lower than those before treatment (P<0.05).The variety of ESR and UA of group C were greater than other groups (P<0.05).The incidence rate of adverse reaction in group A and group C were 5.0% and 7.5% respectively,obviously lower than 22.5 of group B with statistically signficance (P<0.05).The SF-36 score of group C was obviously lower than those of group A and group B with statistically significance (P<0.05).Conclusion:Modified Simiao San combined with colchicine had better effect and safety compared to single medicine,which could greatly improve the quality of life.
6.Allogenic tendon materials and Tibial-inlay technique for posterior cruciate ligament reconstruction of the knee joint in 17 cases
Zhihuai LI ; Zhen FENG ; Yilong ZHANG ; Bo SUN ; Pei WANG
Chinese Journal of Tissue Engineering Research 2010;14(8):1479-1482
BACKGROUND: Donor complications have been detected following autologous tendon transplantation for posterior cruciate ligament reconstruction. Although artificial tendon development and tissue-engineered tendon have achieved great progresses, there are some issues in clinical application. Since 1980's, allogenic tendon transplantation has aroused increasing attention. OBJECTIVE: To explore the selection of allogenic tendon materials and the effect of their application on reconstructing posterior cruciate ligament. METHODS: A total of 17 patients with posterior cruciate ligament injury of knee joint were treated with cryopreserved allogenic tendon by Tibial-inlay technique. During the operation, two tracts of tendons soaked in gentamicin saline for 15 minutes were conduplicated, and one end of the tendon was cancellous bone screw and fixed to the tibia attachment point of posterior cruciate ligament, and the other end was introduced into the joint through retention suture. The posterior joint capsule was repaired. The patient was placed at supine position, and the knee was flexed for 90°. The other end of the graft was introduced to femoral tunnel, and anterior drawer was tensed, and fixed by screw. RESULTS AND CONCLUSlN: The preoperative posterior drawer test of patients was >2+, including 7 cases of 3+ and 6 of 4+. The postoperative posterior drawer test was 0 in 4 cases, 1+ in 8 cases, 2+ in 4 cases and 3+ in 1 case, suggesting the posterior movement of the knee joint was significantly improved. Lysholm scores of patients were (48.5±4.3) points before operation and (88.3±5.4) points after operation. Results show that cryopreserved allogenic tendon by Tibial-inlay technique could restored function of posterior cruciate ligament with a favorable effect.
7.Antibacterial Drugs Consumption During Perioperative Period: An Analysis
Yanping XU ; Jie FENG ; Baoxiang PEI ; Lan LI
Chinese Journal of Nosocomiology 2006;0(06):-
OBJECTIVE To analyze and evaluate the comsumption of the antibacterial drugs during surgery operation in our hospital objectively.METHODS To carry out statistical analysis of antibacterial drugs from May to Dec in 2007.RESULTS The 93 percent of preventive usage of antibacterial drugs were in 2 hours after operation.The most was cephalosporins.CONCLUSIONS It is important to enhance the education of rational use and supervision.
8.Differences between the expression of matrix metalloproteinase-2.9 in preeclampsia and normal placental tissues
Jin-Ke LI ; Qing XIONG ; Shu ZHOU ; Pei-Feng YANG ;
Chinese Journal of Obstetrics and Gynecology 2001;0(02):-
Objective To study the possible relationships between expression of matrix metalloproteinase(MMP)2,9 and the pathogenesis of preeclampsia in which trophoblast invasion is impaired. Methods MMP-2,9 expression were detected by immunohistochemistry streptavidin-biotin complex (SABC)method in 20 normal term placentae and 20 preeclampsia placentae,respectively.In addition, mRNAs for MMP-2,9 were analyzed by real time PCR in both groups.Results The intensities of both MMP-2 and MMP-9 immunostaining in preeclampsia placentae were significantly declined compared to those of normal term placentae(P
9.Homocysteine decreases the invasion in cultured human trophoblasts:relationship between homocysteine and matrix metalloproteinase-2,-9 expression
Pei-Feng YANG ; Jin-Ke LI ; Qing XIONG ;
Chinese Journal of Obstetrics and Gynecology 2001;0(03):-
Objective To test the hypothesis that homocysteine can decrease MMP-2 and MMP-9 expression in cultured trophoblasts of early pregnancy and that homocysteine can prevent trophoblasts invasion in the early stage of preeclampsia.Methods Cytotrophoblasts from early pregnancy were isolated and cultured.Trophoblasts were treated with or without Hcy(1 mmol/L)for 48 hour,and real time RT-PCR and gelatin zymography were used to quantify the mRNA and protease activity of MMP-2,-9.Results Treatment with Hcy(1 mmol/L)induced a decrease in MMP-2 mRNA by 21% and MMP-9 mRNA by 11%.At protein level MMP-2 expression decreased 14% and MMP-9 expression decreased 52% compared with control.Conclusions Homocysteine can decrease MMP-2,-9 expression in trophoblasts of early pregnancy and influence its invasion process.
10.A Brief Review on the Biodegradation of Chloroanilines
Hua-Feng REN ; Shu-Qin LI ; Zhi-Pei LIU ;
Microbiology 1992;0(03):-
Since 1970's , lots of studies on biodegradation of chloroanilines (CAS) have been done, especially, in these aspects: spieces and capability of the microbes; metabolic pathway; gene cloning, expression of degradation plas-mid and pivotal metabolic emzymes. It is necessary for us to review the study on biodegradation of chloroanilines in order to summarize some useful results and the problems in this study.