Amphotericin B ; administration & dosage ; Antifungal Agents ; administration & dosage ; Child ; Cryptococcus neoformans ; drug effects ; pathogenicity ; Diagnosis, Differential ; Drug Delivery Systems ; instrumentation ; methods ; Humans ; Injections, Intraventricular ; Injections, Spinal ; Intracranial Hypertension ; complications ; drug therapy ; etiology ; Male ; Meningitis, Cryptococcal ; diagnosis ; drug therapy ; physiopathology ; Treatment Outcome

Child, Preschool ; Humans ; Lead Poisoning, Nervous System, Childhood ; complications ; Male ; Optic Neuritis ; etiology

Objective To report the outcome of emergency repair traumatized limbs by vascularized free tissue transplantation.Methods From April 1988 to August 2004,86 patients,58 men and 28 women,had undergone emergency vascularized free tissue transplantation to have their injured limbs repaired in 54 cases and the missing thumbs reconstructed in 32.The patients aged from 5 to 55 (mean 27.9) years. The transplants included latissimus dorsi myocutaneons flap,anterolateral femoral skin flap,medial crural skin flaps,dorsal pedal flaps,medial plantar flap,composite tissue mass of the discarded limbs and big toe skin- nail flap.The operations were performed 1 to 5 days after injuries.Results Postoperative vascular crises occurred in 8 cases and were all followed by exploration with successes in 5 cases while failure in 3.The total survival rate of the transplants was 96.5% (83/86).In this series all the patients were followed up for 1 to 16 years with a mean of 7.5 years only to reveal satisfying functional recovery in all the repaired limbs and an ex- cellent and good rate of 87.5% in the reconstructed thumbs.Conclusion Emergency vascularized free tis- sue transplantation is an effective way to repair a traumatized limb and to reconstruct a traumatically missing thumb.

Objective To explore the methods for coverage of the defect of great toe after the wrap-a- round flap transferand decrease the morbidity of donor site in great toes.Methods Twenty-five patients received three kinds of procedure for immediate resurfacing of donor defect of the great toes during wrap-around flap transferAmong them9 cases received the free flaps for coverage of defect in donor great toes12 cases was repaired by local pedieled dorsal or plamarpedis flapsand the other cases were treated by the nail-flap of second toe.Results All the flaps were survivalTwo patients received the flap thinning procedure in 6 months laterall patients were satisfied with cosmetic and functional outcomeThe appearance and sensory function of donor toe repaired by second toe nail-flap was best among three methods.Conclusion Accord- ing the detect situation of great toesthree kinds of flap were selected for immediate coverage of donor site, which can decrease the complication of donor great toe at the most.

OBJECTIVEThis study examines the impacts of an improved electrode placement on the electrocardiogram (ECG) results in order to determine a better electrode placement for ECG monitoring in children.

METHODSECG was recorded using the traditional electrode placement and the modified electrode placement (with shortened electrode distance) respectively in 50 pediatric patients. The amplitudes of P wave and QRS wave on ECG by the two measurements were compared. Furthermore, the impacts of different body positions on the amplitudes of P wave and QRS wave were studied after applying the modified electrode placement.

RESULTSThere were no significant differences in the amplitudes of P wave and QRS wave on ECG by the traditional electrode placement and the modified electrode placement (P>0.05). When modified electrode placement was utilized, the body position change did not lead to significant changes in the amplitudes of P wave and QRS wave (P>0.05).

CONCLUSIONSA satisfactory ECG can be obtained with the modified electrode placement independent of patient's body position, suggesting that the modified electrode placement can be used instead of the traditional placement in children.

Child ; Child, Preschool ; Electrocardiography ; instrumentation ; Electrodes ; Female ; Humans ; Male ; Monitoring, Physiologic ; Patient Positioning

OBJECTIVETo explore the regulative effects and significance of neuropeptide substance P (SP) on the expression of basic fibroblast growth factor (bFGF) of granulation tissue fibroblasts in vitro.

METHODSA local aseptic inflammation was induced by injection of formaldehyde in rats, and its granulation tissue was cultured. RT-PCR was employed to observe expression of bFGF mRNA after inducement of SP at different concentrations and time points in the granulation tissue, and western blot to assay expression of bFGF protein.

RESULTSThe expression of bFGF mRNA was markedly increased significantly 3 and 6 hours after inducement with SP in 10(-7) mol/L, compared with control group (P < 0.01). The expression of bFGF protein was markedly higher than the control group after 12 hours, and it reached the peak at the 24th hour and declined gradually after 48 hours. SP at concentrations of 10(-9) - 10(-5) mol/L could significantly promote the expression of bFGF mRNA, and that at 10(-8) - 10(-5) mol/L induce the expression of bFGF protein. Both expressions reached the peak when SP concentration was 10(-7) mol/L (P < 0.01).

CONCLUSIONSP can induce the expressions of bFGF mRNA and bFGF protein of granulation tissue fibroblasts in vitro, which may possess an important significance in wound healing.

Animals ; Cells, Cultured ; Fibroblast Growth Factor 2 ; metabolism ; Fibroblasts ; drug effects ; metabolism ; Granulation Tissue ; drug effects ; metabolism ; Male ; RNA, Messenger ; metabolism ; Rats ; Rats, Wistar ; Substance P ; pharmacology ; Wound Healing ; drug effects

OBJECTIVETo explore the proliferation-promoting effect of sensory neuropeptide substance P (SP) on the cultured granulation tissue fibroblasts in vitro and its regulative effect on the gene expression of basic fibroblast growth factor (bFGF) mRNA.

METHODSThe proliferation-promoting effect of cultured granulation tissue fibroblasts was observed by means of MTT; the regulative effect of SP on gene expression of fibroblast bFGF by RT-PCR. The time and dose-efficiency relations were also observed.

RESULTSThere was a significant proliferation-promoting effect of SP on the cultured granulation tissue fibroblasts in vitro in a remarkable dose-dependent fashion. However, bFGF antibody only partly exerted its inhibitive effect. SP could induce the bFGF mRNA expression of the fibroblasts at the 3rd and 6th hour (P < 0.01). SP could promote the bFGF mRNA expression of the fibroblasts in the concentration of 10(-9) - 10(-5) mol/L and peaked in the concentration of 10(-7) mol/L.

CONCLUSIONSSP has a significant proliferation-promoting effect on the granulation tissue fibroblasts, which is correlated with SP inducing bFGF mRNA expression of fibroblasts.

Animals ; Cell Division ; drug effects ; Dose-Response Relationship, Drug ; Fibroblast Growth Factor 2 ; genetics ; Fibroblasts ; cytology ; drug effects ; metabolism ; Gene Expression ; drug effects ; Granulation Tissue ; cytology ; drug effects ; metabolism ; Male ; RNA, Messenger ; genetics ; metabolism ; Rats ; Rats, Wistar ; Reverse Transcriptase Polymerase Chain Reaction ; Substance P ; pharmacology

Acute-Phase Proteins ; physiology ; Carrier Proteins ; physiology ; Humans ; Membrane Glycoproteins ; physiology ; Receptors, Cell Surface ; physiology ; Receptors, Immunologic ; physiology ; Receptors, Scavenger ; Sepsis ; metabolism ; physiopathology ; Signal Transduction ; Toll-Like Receptors

OBJECTIVETo investigate the frequencies of -1470, -511 and -31 single nucleotide polymorphisms (SNPs) in the promoter of IL-1beta and its haplotype constitution in Chongqing population.

METHODSOne hundred and twelve healthy Chongqing people were enrolled in this study. Polymorphisms at -1470 (G to C), -511 (T to C) and -31 (C to T) of IL-1beta were genotyped with the method of restriction fragment length polymorphism (RFLP). Haplotype frequencies were analyzed by Arlequine software.

RESULTSFrequencies of IL-1beta -1470, -511 and -31 SNPs were 41.67%, 50% and 45.33%, respectively. Genotype frequencies of -1470 locus were 39.81%, 37.04% and 23.15% for G/G, G/C and C/C respectively. As for T-511C SNP, genotype frequencies of T/T, T/C and C/C were 29.91%, 40.18% and 29.91%, respectively. Genotyping results of C/C, C/T, and T/T of -31 locus were 35.51%, 38.32% and 26.71% respectively. Haplotype analysis found that there were mainly three haplotypes constituted by three SNPs, ie., G-T-C, C-T-C and G-C-T.

CONCLUSIONSPolymorphisms exist in the promoter of IL-1beta in Chongqing population. Three SNPs locate in the same haplotype block.

Asian Continental Ancestry Group ; genetics ; China ; Cohort Studies ; Female ; Genetics, Population ; Genotype ; Haplotypes ; Humans ; Interleukin-1 ; genetics ; Male ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Polymorphism, Single-Stranded Conformational ; Promoter Regions, Genetic ; Sensitivity and Specificity

OBJECTIVETo investigate the feasibility and effectiveness of laparoscopic radical trachelectomy and lymphadenectomy in the treatment of early-stage cervical cancer.

METHODSThe clinical data of 6 patients (stage 1a2 to 1b1), who underwent laparoscopic fertility-preserving radical operation for cervical cancer in our department from February 2009 to October 2010, were retrospectively analyzed in terms of operation duration, intra-operative blood loss, postoperative pathology, complications, and pregnancy.

RESULTSBoth radical resection of cervical and pelvic lymph node dissection were completed under laparoscopy, and only the cervical and vaginal cuffs were closed from vagina. The operation duration ranged 155-210 min (mean: 185 min) and the intra-operative blood loss was approximately 60-120 ml(mean: 105 ml). The average length of hospital stay was 18 days without complications, postoperative infection, and bleeding. Postoperative pathology showed no lymph node metastasis, and no ligament, blood vessels, vaginal cutting margin, or upper part of cervix was invaded by tumor cells. During the 8-20-month follow-up, 1 patient had become pregnant for 4 months and no case experienced tumor recurrence.

CONCLUSIONLaparoscopic fertility-preserving lymphadenectomy and radical trachelectomy is feasible for patients with early-stage cervical cancer who have strong wish to have a child.

Adult ; Female ; Fertility Preservation ; Follow-Up Studies ; Humans ; Hysterectomy ; methods ; Laparoscopy ; Retrospective Studies ; Treatment Outcome ; Uterine Cervical Neoplasms ; surgery ; Young Adult