OBJECTIVE:To provide references for drugstores to launch pharmaceutical care.METHODS:The consents,methods and obstacles in launching pharmaceutical care for drugstores were analyzed.RESULTS&CONCLUSION:The launching of pharmaceutical care in drugstores can not only considerably ensure the benefits of consumers in drug use,but also raise their own core competitive power.

OBJECTIVE: To establish an analytical method of ultra performance liquid chromatography-quadrupole/orbitrap high resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) for identification and quantitation of the multi-constituents of Danshen-chuanxiongqin injection. METHODS: The method of UHPLC-HRMS was developed to identify the complex chemical composition and full ms scan mode was used to determine the main active ingredients precisely, then the means of principal component analysis (PCA) was used to comprehensively assess the quality of Danshen-chuanxiongqin injection. RESULTS: A total of 23 chemical constituents were identified in Danshen-chuanxiongqin injection, 14 of which were unambiguously identified by comparing with chemical standards, and seven major compounds, ligustrazine, danshensu, salvianolic acid A, succinic acid, rosmarinic acid, protocatechuic aldehyde, caffeic acid, in Danshen-chuanxiongqin Injection were determined accurately. The analysis by multivariate data processing software SIMCA 14.0 indicated that the quality of most batches of samples were stable except ligustrazine, danshensu, and salvianolic acid A which were a bit unstable in some individual batches, and it was essential to monitor the content of salvianolic acid A to control the drug quality. CONCLUSION: The established HPLC-TOF-MS/MS method has the properties of rapid identification, high specificity, simpliness, and feasibility, which can be used as a better means for the qualitative and quantitative analysis of danshen-chuanxiongqin injection. Moreover, it lays the ground for quality control of the drug and provides data reference for the rational use in clinic.

Objective To preliminarily establish the reference interval of serum non‐esterified fatty acids(NEFA)among popula‐tion in Wuhan area by enzymatic assay .Methods NEFA level of serum samples from A total of 1 250 individuals undergoing healthy physical examination in our hospital were selected as the research subjects .The Olympus AU5400 biochemistry analyzer and SEKISUI reagents were adopted to detect serum NEFA level .The regression analysis was adopted to analyze the NEFA influencing factors .The NEFA 95% reference interval was estimated by using the normal distribution method and the reference interval was verified .Results The regression analysis showed that the body mass index (BMI)and age had significant effect on NEFA (P<0 .01) .NEFA was highest in the group aged ≥60 years old ,followed by the group of 18- <45 years old ,and lowest in the group of 45-60 years old ;NEFA was highest in the group of BMI<18 .5 kg/m2 .The 95% reference interval of NEFA for healthy popula‐tion in Wuhan area was estimated as 204 .6 -975 .2 μmol/L .The detected NEFA level for clinical verifiers was 242 .0 -831 .2μmol/L ,which conformed to the above reference interval .Conclusion The serum NEFA reference interval among healthy popula‐tion in Wuhan area is primarily established .

Objective To evaluate the safety of CT guided percutaneous lung biopsy in the elderly,and to analyze the risk factors for its complications.Methods Totally 204 elderly patients aged ≥65 years underwent transthoracic needle biopsy under CT guidance.Clinical data,complication and its risk factors were analyzed retrospectively.Results The complications included pneumothorax accounting for 15.2％ (31/204),hemoptysis 21.6％ (44/204),and no mortality was found.Logistic regression analysis showed that needle path ≥5.00 cm,lesion size ≤2.00 cm and emphysema around the lesion were the independent risk factors for postoperative pneumothorax (OR=4.05,2.54 and 3.97,all P＜0.05).Meanwhile,the enhancement degree by CT ＞40 HU,needle path ≥5.00 cm and lesion size ≤2.00 cm were the independent risk factors for postoperative bleeding (OR=2.27,2.82 and 2.33,all P＜0.05) in elderly patients undergoing lung biopsy.The sensitivity and specificity for postoperative pneumothorax and bleeding obtained by forecasting equation were 87.1％ and 49.1％,54.5％ and 80.8％ respectively.Conclusions The length of needle path,lesion size and emphysema around the lesion are correlated with pneumothroax,and length of needle path,lesion size and the CT enhancement degree are correlated with bleeding after percutaneous lung biopsy in the elderly.

Objective:To explore the mediating effect of loneliness on the relationship between interpersonal adaptation and mobile phone addiction in college students.Methods:Totally 368 college students (176 males and 192 females )were surveyed with the College Student Adaptability (CSAI),University of Califomiaat Los Angels (UCLA)and Mobile Phone Addiction Index (MPAI).Then AMOS18.0 was used to establish structural equation modeling (SEM)among interpersonal adaptation,loneliness and mobile phone addiction,and test the mediating effect of loneliness on the relationship between interpersonal adaptation and mobile phone addiction in college students.Results:The MPAI scores were significantly higher in the low interpersonal adaptation group (the score of CSAI less than 21 )than in the high interpersonal adaptation group (the score of CSAI higher than 32)(P<0.001).The scores of interpersonal adaptation were negatively correlated with scores of loneliness and mobilephone addiction (r =-0.71,-0.25,P﹤0.01).The scores of loneliness were positively correlated with scores ofmobile phone addiction (r =0.32,P ﹤ 0.01).College students'interpersonal adaptation did not have significantnegative predictability for mobile phone addiction (β=﹣0.08,P =0.32),but it had significant negative predictabilityfor loneliness (β=﹣0.71,P <0.001).Loneliness had significant positive predictability for mobile phone addiction(β=0.29,P <0.001).Conclusion:The results show loneliness completely plays a mediating effect betweeninterpersonal adaptation and mobile phone addiction.

Objective To define the CT imaging characteristic of menetrier disease. Methods Four patients with proved Menetrier disease were retrospectively studied by means of CT performed with the water as oral contrast, hypotonic agent and dynamic or spiral CT technique. Results All cases showed that the enlarged rugae were seen projecting into the gastric lumen, but the gastric wall was otherwise normal. CT detected all lesions and made a confirmed diagnosis before pathology biopsy. Conclusion Our primary result revealed that the CT appearance of Menetrier disease was quite characteristic, CT is a very valuable tool for the diagnosis of Menetrier disease.

Eighteen Chinese mongrel dogs were divided into 3 groups randomly according to the given environment: normal environment (NE) group, hot and humid environment (HHE) group, heat acclimatization (HA) group. The dynamic changes in the lymphocyte subsets with CD4 + , CD8 + ,CD11a + ,CD11b + expressions and neutrophil with CD11a and CD11b expressions in peripheral blood were detected post trauma using mAb FACS immuno fluerescence technique. In HHE group, there was a temporary increase (1h) and prolonged decrease (10h) in CD4 + subset, with an increase in CD8 + subset (4h later), resulting in final decrease in CD4 + /CD8 + ratio. In addition, CD11a + T cell decreased 8h later, with a mild change in CD11b + subset. The mechanism is unclear presently. Adaptive training seemed to raise the level of CD4 + and CD11a + lymphocyte subsets and suppress the expression of CD8 + subset. The expression of CD11a and CD11b in NE group increased immediately after injury, presenting a second peak at 4h. The second peak with a higher level was also observed in HHE group, but its duration was shorter than that of the NE group. Heat acclimatization lowered the level of the second peak, though it was remarkably prolonged. Changes in lymphocyte subsets and adhesion molecules of neutrophil suggested that hot and humid environment might suppress the immunity after gunshot injuries, while adaptive exercise could blunt the immunosuppressive response.

Arsenic ; urine ; Chromatography, High Pressure Liquid ; methods ; Formamides ; analysis ; Humans ; Urinalysis ; methods

Objective To explore the chemotherapeutic agents-induced modulating effects of Egr-1 promoter sequence on GM-CSF expression and its protective effect against injury to haematopoiesis due to chemotherapy.Methods Human GM-CSF cDNA and enhanced green fluorescent protein(EGFP) cDNA were linked to internal ribosome entry site(IRES) respectively,and then recombined to pCIneo vector containing Egr-1 promoter(Egr-EG).The vector was transferred into human bone marrow stromal cell line HFCL by lipofection,and the HFCL/EG cells were then finally constructed.MTT assay was performed to determine the effects of cisplatin(DDP),5-fluorouracil(5-FU),gemcitabine(GEM) and paclitaxel(PTX) on the survival rates of HFCL/EG and HFCL cells,and the IC50 of such agents against HFCL/EG and HFCL cells were calculated.The percentage of HFCL/EG cells which positively expressed EGFP was assessed by both flow cytometry and inverted fluorescence microscope.The effects of the active oxygen inhibitor N-acetylcysteine(NAC) on the expression of GM-CSF,which was modulated by promotor Egr-1,were detected by ELISA.The effects of HFCL/EG supernatant on CFU-GM after being exposed to chemotherapeutic agents were examined.Results With different sensitivity to DDP,5-FU,GEM and PTX,HFCL/EG cells were successfully constructed.The drugs showed higher IC50 value against HFCL/EG cells than HFCL cells(P