Objective To compare the effect of ureteroscopic pneumatic lithotripsy(URL) and extracorporeal shock wave lithotripsy(ESWL) for ureteral stones.Methods 90 patients with ureteral stones were randomly divided into group A( URL group),group B( ESWL group),each group 45 cases.The effect of surgical treatment was compared between the two groups.Results The one-time success rate of gravel,stone free after 4 weeks of A group was significantly higher than that of group B( x2 =9.680,5.414,all P ＜0.05),but there was no significant difference in the surgical time and hospital stay of the two groups( t =1.010,0.909,all P ＞ 0.05 ).The postoperative renal colic,fever and other complications of group A were significantly lower than that of group B ( x2 =6.049,5.075,all P ＜0.05),but the gross heraturia and ureteral injury of group A was higher than that of group B(x2 =5.874,3.873,all P ＜ 0.05 ).Conclusion The ureteroscopic pneumatic lithotripsy and extracorporeal shock wave lithotripsy in the treatment of ureteral stones all have good effect,but ureteroscopic pneumatic lithotripsy and stone rubble drain success rate are significantly higher than extracorporeal shock wave lithotripsy,and postoperative complications are relatively less than extracorporeal shock wave lithotripsy.

ObjectiveTo investigate the delayed umbilical cord clamping in preterm children of anemia in premature children.Methods60 preterm children were randomly divided into study group and control group 30 patients in each group.The control group was taken regular off umbilical,the observation group delay 90s(after birth at least 2min) off umbilical.ResultsThe Hb, HCT, and SI in the postnatal 1,2,4,8 weeks were significantly lower than the observation group, the difference was statistically significant (all P ＜ 0.05), and the control group after birth in preterm children Ret in the first week was significantly higher than 2,4 in the observation group, the difference was statistically significant(all P ＜ 0.05) ; The observation group at 6 months the incidence of anemia(13.3％) and transfusion rate(6.7％) were significantly lower than the control group(43.3％ ,33.3％) ,the difference was statistically significant(all P ＜0.01).ConclusionDelyed umbilical cord clamping in preterm children could increase the redblood cells and iron reserves in preterm children, reduce the incidence of anemia caused by blood transfusion, was worthy of clinical application.

Objective To analyse the clinical value of aspartate aminotransferase mitochondrial isoenzyme (m‐AST ) determina‐tion in patients with alcoholic liver disease .Methods 61 cases of patients with alcoholic liver disease (observe group) receiving treatment in this hospital from March 2013 to March 2015 and 61 cases of healthy individuals(control group) were enrolled in this study ,and serum levels of m‐AST ,alanine amino transferase(ALT) ,aspartate amino transferase(AST) and γ‐glutamyl transferase (GGT) were detected .Results Compared with the control group ,serum levels of of m‐AST ,ALT ,AST and GGT were increased in the observe group ,there were statistically significant differences (P<0 .05) .In patients with alcoholic liver disease ,after treatment , serum levels of m‐AST ,ALT ,AST and GGT were different among patients with hepatic adipose infiltration ,patients with hepatitis and patients with liver cirrhosis ,there were statistically significant differences(P<0 .05) .Conclusion Combined with ALT ,AST and GGT ,m‐AST could be utilized as an indicator for diagnosing alcoholic liver disease ,which has clinical significance .

Tissue engineering has been one of the most challenging and rapidly developing frontier fields of science in the recent 20 years. Being referred to as “a medical revolution of far-reaching significance”, “a new epoch of regenerative medicine”, it signals the coming of a period of reproduction of regenerative tissue and organs. It has drawn intensive attention and support from medical circles and governments all over the world because of its advantages in trauma repair, functional reconstruction, life saving and improving quality of life, as well as its huge social and economic values. Products of tissue engineered skin and cartilage with approval of the American FDA have been available. Hundreds of tissue engineering companies and research centers have been set up both abroad and at home. It is now possible to construct tissue-engineered tissues. Moreover, projects of complex organ construction by means of tissue engineering is being carried out vigorously in the world. Some tissue-engineered tissues have been put into trial use in clinic and resulted in encouraging outcome. All these forecast a bright and promising prospect for tissue engineering. As a new branch of science and technology that involves multiple specialties and fields, however, it is still at its initial stage. Many fundamental scientific problems are to be explored, and many technical difficulties to be overcome. Hope and difficulty coexist; opportunity and challenge stand side by side. We should seize the opportunity and meet the challenge with our creative and strenuous efforts to make tissue engineering benefit human beings as early and much as possible.

PET is the most advanced nuclear medical imaging equipment. This paper introduces the characteristics, application and trend of PET.

Objective:To investigate the possibility and the usefulness of group psychological guidance in mental health education course. Methods:Two classes of the same grade were chosen,one was group psychological guidance class,and the other as control. SCL-90 was used to measure the effect of group instruction. Results:after group intervention, the study group decreased more in SCL-90 total score than control (24.5?7.2/13.4?2.5,t=6.24,P

Objective:To study the incidence,risk factors and clinical manifestaion for ectopic pregnancy(EP)after in vitro fertilization and embryo transfer(IVF-ET). Methods:A retrospective study of IVF and intracytoplasmic sperm injection (ICSI) pregnancies was performed between January 2002 and July 2005. Results:A total of 668 woman conceived after IVF or ICSI were studied. The rate of EP was 8.2%,corresponding with 55 Eps,of which 15 were heteropic pregnancy(HP). There were 40 Hps in tubal facter group,There apper to be no relationship between d2 ET and d 3 ET,ovulation induction and age. Conclusions:Tubal pathology is associated risk of EP.

Objective:To establish a new fluorescence-based quantitative PCR assay for detecting hepatitis B virus covalently closed circular DNA(HBV cccDNA).To explore the effect of artificial liver support system(ALSS)on total HBV DNA and HBV cccDNA in sera of patients with fulminant hepatitis B.Methods:The serum specimens from 50 patients of fulminant hepatitis B prior to and after treatment with ALSS were collected.Then HBV cccDNA was quantitatively detected by fluorescent PCR with specific primer set and Taqman probe.And the results were analyzed by SAS8.0 statistics software.Results:We successfully established a new fluorescence-based quantitative PCR method for HBV cccDNA.HBV cccDNA was detectable in sera of the patients with fulminant hepatitis B.The level of total HBV DNA and HBV cccDNA decreased significantly following plasma exchange in ALSS.The levels of total HBV DNA and HBV cccDNA had positive correlation with degree of PT,ALT,andAST.Total HBV DNA also had positive correlation with HBV cccDNA.Conclusion:The level of total HBV DNA and HBV cccDNA has positive correlation with degree of PT,ALT,and AST,which might be indication of hepatocyte damage.The finding that significant decrease in total HBV DNA and HBV cccDNA after plasma exchange in ALSS may provide a clue of further research about the fulminant hepatitis B treated with ALSS.

Objective:To investigate the outcomes,complications and safety about Endoscopic thoracic sympathectomy for palmar hyperhidrosis.Methods:Analyzing the results and complications about the 336 cases,who were received Endoscopic thoracic sympathectomy for treating palmar hyperhidrosis from Jan.2005 to Sep.2008,and were followed up.Results:All of their hands became dry and warm after operation.326 patiens were followed-up for 1 to 45 months,and 25.4 months on average.The total Results:No recurrence.Compensatory hyperhidrosis was found in 42 cases(12.88%).Conclusions:It is effective,safer and mini-trauma that Endoscopic thoracic sympathectomy for treating palmar hyperhidrosis.

Objective To analyze the results of human papillomavirus(HPV)DNA genotyping detection in 312 female outpatients attending STD clinics.Methods HPV DNA genotyping was detected by fluorescent quantitative polymerase chain reaction(FQ-PCR)for 312 samples collected from female outpatients attending STD clinics.Results The HPV detection rates in outpatients with HPV in their neoplasm and secretion were 83.8% and 38.1%,respectively,and the difference between the two groups had statistical significance.Of the 111 cases with visible neoplasm 4,5 and 57 cases were with 16,18 and 6/11 single type HPV infection,respectively,while 0,16,8 and 3 cases were.with 16+18,16+6/11,18+6/11 and 16+18/11 multiple type infection,respectively.Of the 201 cases with abnormal secretion including cases with other STDs,cases with CA and other STDs in their spouses,treated cases with CA,and cases asking for self-examination because of their risky sex behavior,15,12 and 43 were with 16,18,and 6/11 single type infection,respectively,while 2,5,1 and 0 cases were with 16+18,16+6/11,18+6/11,16+18+6/11 multiple type infection,respectively.Conclusions High HPV DNA rates were detected in neoplasm and secretion of female STD outpatients;although the low risk 6/11 type HPV infection was the main cause,the common high risk type HPV infection was also detected.Therefore,routine HPV DNA detection should be provided to this population group.