Objective: To study the protective effects of canthaxanthin against decreasing of i mmuno-function of the acute lung injury induced by lipopolysaccharide in mice and research its possible mechanism for offering scientific basis of its health function. Methods: Randomly distribute 60 male Kunming mices into control group, lipopolysaccharide-induced acute lung injury model group(ALI), dexamethasone (DXM) group (5 mg/kg) and low, middle, high dose groups of canthaxanthin (10 mg/kg, 15 mg/kg, 20 mg/kg). After 30 d feed by different dosage canthaxanthin, control group were given physiological saline, ALI model group, canthaxanthin administrated groups and DXM group were injected with lipopolysaccharide (LPS) (6.0 mg/kg) to induce ALI. Six hours after LPS or physiological saline challenged, abdominal aorta blood for measuring lymphocyte subpopulations(CD3+, CD4+, CD8+), tumour factor-?(TNF-?), leckocyte interpose-8(IL-8), malondialdehyde (MDA) content, superoxide dismutase (SOD), glutathione perioxidase (GSH-Px) activities, and lung wet weight/dry weight ratio, neutrophil MPO activity of lung, tumour factor-?(TNF-?), leckocyte interpose-10 (IL-10) content of lung. Results: Treatment with different dosage canthaxanthin could significantly decrease serum TNF-?, IL-8, MPO, MDA content and lung wet weight/dry weight ratio, while inhibit the reduction of IL-10 in lung and SOD, GSH-Px activities, improve the percent of lymphocyte subpopulations. Conclusion: Canthaxanthin showed protective effects on immuno-function of the acute lung injury induced by LPS in mice.

This article summarized clinical studies and mechanism research literature on acupuncture in treating chemotherapy induced nausea and vomiting in the latest 20 years. It found that the domestic and foreign researches tend to regard acupuncture as an effective method for the treatment of chemotherapy induced nausea and vomiting, which still requires further high-level clinical researches to verify. Acupuncture may prevent and treat chemotherapy induced nausea and vomiting through multiple mechanisms, and studies on these mechanisms show extensive research prospect.

Recently,the incidence of Alzheimer's disease has been significantly increasing.However,the pathogenesis of Alzheimer's disease remains unknown.It is presumed that insulin and insulin receptor may be involved in the pathogenesis of Alzheimer's disease.The relationship between insulin and cerebral glucose metabolism,the relationship between insulin,insulin receptor,insulin resistance and pathogenesis of Alzheimer's disease as well as the possible mechanism are reviewed in this paper,and the progress of treatment of AD is also described.

Myelodysplastic syndromes ( MDS ) represent a heterogeneous group of clonal hematopoietic disorders with diverse clinical course .Because of the heterogeneity and the lack of molecular markers to monitor disease progression, clinical management of MDS patients is challenging .Recently, with the development of molecular analysis techniques , an increasing number of MDS related molecular biomarkers have been reported.In this review, we will discuss the clinical applications of the newly reported molecular makers in terms of diagnosis , prognosis and treatment.These markers may improve the diagnosis and prognostic assessment systems of MDS , which may potentially be used to guide decision making in the individual therapy.

The mutation status of calreticulin gene (CALR) is helpful for the diagnosis of JAK2 / MPL mutation-negative myeloproliferative neoplasms (MPN), and is closely related to the MPN progression and prognosis.Currently, Sanger sequencing, PCR-fragment analysis, high resolution melting, Taqman RQPCRand the next generation sequencing have been reported to be used to detecting the CALR gene mutations.A proper method for CALR mutation detection and a right laboratory diagnostic procedure according to the MPN-related molecular markers will facilitate the rapid and effective diagnosis and treatment of MPN.

Objective To explore the association between the polymorphism in IL-28B gene and the interferon virological response in HBeAg-posi-tive chronic hepatitis B patients. Methods A literature search was performed on PubMed,web of Science,China Bio Medicine(CBM)and wan-Fang database. The references was screened with the inclusion and exclusion criteria,and then data were extracted and methodological quality of the studies was judged. Statistical analysis was carried out with RevMan 5.3 and STATA 11.0. Results Totally 7 publications were recruited for the study,including 1 345 patients. The meta-analysis showed that there was no significant difference of interferon response between HBeAg-positive chronic hepatitis B patients with rs8099917TG/GG alleles and rs8099917TT alleles (P= 0.20). There was no statistical difference between Rs12979860CC and CT/TT allele for the sustained virological response(P=0.75). Conclusion Our current meta-analysis suggests that there was no correlation of rs8099917,rs12979860 polymorphism in IL-28B with the sustained virological response to interferon in HBeAg-positive chronic hepatitis b.

Blood Vessels ; physiology ; Humans ; Kruppel-Like Transcription Factors ; metabolism ; physiology

Aminopeptidase N,a zinc-dependent exopeptidase,is highly expressed in many kinds of tumors,which involves in the degradation of extracellular matrix barriers and angiogenesis and promotes the invasion and metastasis of cancer cells.Aminopeptidase N inhibitors can induce apoptosis and inhibit the invasion and metastasis of tumor cells,which has become an attractive target for anti-tumor therapy.

Objective To evaluate the expressive levels of calcitonin gene related peptide (CGRP) and neuropeptide Y (NPY) in tissue-engineered bone with fascia flap in vivo. Method A segmental bone defect 1.5cm in length was made at the both radius of 12 healthy New Zealand rabbits. The defects were repaired by implantation in two ways: for left radius, engineered bone with fascia flap was implanted (served as experiment group), and for right radius only engineered bone was implanted (served as control group). 3, 6 and 12 months after implantation, the expressions of CGRP and NPY in the new bone were determined with immunohistochemistry and semi-quantified using image analysis software. Results CGRP and NPY expressions in the both groups were significantly increased in a time-dependent manner (P

In this research, phosphate and thiophosphate prodrugs 3a, 3b of anti-HIV agent AZT were synthesized, and their anti-HIV activities and cytotoxicities were investigated in vitro. Results showed that the prodrugs 3a and 3b with an IC50 value of 11.0 and 4.0 micromol x L(-1), respectively, were less toxic than AZT (1.0 micromol x L(-1)). Although the EC50 values of both 3a (0.04 micromol x (L(-1) and 3b (0.16 micromol x L(-1)) were lower than that of AZT (0.01 micromol x L(-1)), the therapeutic index (IC50/EC50) of prodrug 3a (275) was much higher than that of both AZT (100) and prodrug 3b (25). This indicated that the prodrug 3a merited further investigation as an anti-HIV agent.