Carcinoma in Situ ; metabolism ; pathology ; Carcinoma, Endometrioid ; metabolism ; pathology ; Cystadenocarcinoma, Serous ; metabolism ; pathology ; Endometrial Neoplasms ; metabolism ; pathology ; Female ; Humans ; Tumor Suppressor Protein p53 ; metabolism

ObjectiveTo investigate the delayed umbilical cord clamping in preterm children of anemia in premature children.Methods60 preterm children were randomly divided into study group and control group 30 patients in each group.The control group was taken regular off umbilical,the observation group delay 90s(after birth at least 2min) off umbilical.ResultsThe Hb, HCT, and SI in the postnatal 1,2,4,8 weeks were significantly lower than the observation group, the difference was statistically significant (all P ＜ 0.05), and the control group after birth in preterm children Ret in the first week was significantly higher than 2,4 in the observation group, the difference was statistically significant(all P ＜ 0.05) ; The observation group at 6 months the incidence of anemia(13.3％) and transfusion rate(6.7％) were significantly lower than the control group(43.3％ ,33.3％) ,the difference was statistically significant(all P ＜0.01).ConclusionDelyed umbilical cord clamping in preterm children could increase the redblood cells and iron reserves in preterm children, reduce the incidence of anemia caused by blood transfusion, was worthy of clinical application.

Objective To analyze the results of human papillomavirus(HPV)DNA genotyping detection in 312 female outpatients attending STD clinics.Methods HPV DNA genotyping was detected by fluorescent quantitative polymerase chain reaction(FQ-PCR)for 312 samples collected from female outpatients attending STD clinics.Results The HPV detection rates in outpatients with HPV in their neoplasm and secretion were 83.8% and 38.1%,respectively,and the difference between the two groups had statistical significance.Of the 111 cases with visible neoplasm 4,5 and 57 cases were with 16,18 and 6/11 single type HPV infection,respectively,while 0,16,8 and 3 cases were.with 16+18,16+6/11,18+6/11 and 16+18/11 multiple type infection,respectively.Of the 201 cases with abnormal secretion including cases with other STDs,cases with CA and other STDs in their spouses,treated cases with CA,and cases asking for self-examination because of their risky sex behavior,15,12 and 43 were with 16,18,and 6/11 single type infection,respectively,while 2,5,1 and 0 cases were with 16+18,16+6/11,18+6/11,16+18+6/11 multiple type infection,respectively.Conclusions High HPV DNA rates were detected in neoplasm and secretion of female STD outpatients;although the low risk 6/11 type HPV infection was the main cause,the common high risk type HPV infection was also detected.Therefore,routine HPV DNA detection should be provided to this population group.

The research on war injuries in tropical areas is an important subject to be urgently dealt with at present in Chinese military medicine. Military medicine related to hot and humid environment is a significant branch of military environmental medicine. More efforts should be made in the research on care of war injuries as well as on military hygiene and military epidemiology so as to establish an integrated system involving basic and clinical science and art in military environmental medicine in hot and humid areas, which should be considered as an important task in the transition of our military strategy to win a war with improvement in the military medical support capability in a local high technicology conflict in such an area. In this paper, the significance of paying more attention to the research on the care of war injuries in hot and humid areas is emphasized, their features are highlighted, and main problems demanding further research are proposed.

AlM: To evaluate the clinical effect of implantation of phakic anterior chamber iris clip intraocular lens in high myopia patients.
METHODS: Fifty eyes of 28 high myopia patients with anterior chamber phakic Verisyse iris clip intraocular lens implantation were observed. The visual acuity, best corrected visual acuity, intraocular pressure, corneal and anterior chamber inflammation changes were observed after the procedure.
RESULTS: Fifty eyes of 28 high myopia patients were successfully accepted implantation of phakic anterior chamber iris clip intraocular lens. Visual acuity of all the patients was increased after operation. And one year later, visual acuity of 38 eyes ( 76%) ≥1. 0, 12 eyes (24%) ≥0. 5, best corrected visual acuity of 40 eyes (80%) ≥1. 0, 10 eyes ( 20%) ≥0. 5. ln 22 eyes, the postoperative best corrected visual acuity were reached and exceeded preoperative best corrected visual acuity.CONCLUSlON: lmplantation of iris clip intraocular lens in high myopia patients with anterior chamber lens was feasibility and effective.

Thyroid associated ophthalmopathy (TAO) is often shown as upper eyelid retraction,conjunctival hyperemia and edema,proptosis,diplopia,strabismus and decreased visual acuity.It is difficult to make a certain diagnosis in the early stage of TAO.Patients suffering from TAO often have upper eyelid retraction at the early stage of disease,which affects appearance and causes feelings of discomfort and decreased visual acuity.Learning more about upper eyelid retraction contributes to the early diagnosis and treatment of TAO.Although upper eyelid retraction with TAO has distinctive manifestations,it is easy to misdiagnose.The differential diagnosis includes congenital eyelid retraction,neurological disorders,high axial myopia,glaucoma filtering bleb,and so on.Family history,clinical manifestations and TAO related examinations should also be taken into consideration.Comprehensive treatments should be adopted.Glucocorticoids and eyelid retraction surgeries are current mainstay of treatment.There are controversies on the application of glucocorticoids about the specific dosage,while agreement achieved that the cumulative dose should not be more than 8 g in one stage in order to reduce the risk of liver failure and other adverse events.Different operation methods should be chosen according to the degrees of retraction and causes of disease for each type of patients.In addition,special attentions should be paid to the decrease of muscle strength in the temporal side.Individual analysis is needed in order to diagnose and treat upper eyelid retraction with TAO.Early diagnosis and intervention can improve the appearance,visual function and quality of life of the patients.

Objective To establish and validate a method for the determination of the interesting protein expression level of recombinant adeno-associated virus(rAAV)infected cells,so as to monitor the product quality in different stages of rAAV9production process.Methods After incubation of serial diluted rAAV samples with infection enhancer Envirus-AAV,the human malignant glioblastoma cells(U87-MG)pretreated with hydroxyurea(HU)were infected.Using rAAV9 reference as the standard,the expression level of glutaryl-CoA dehydrogenase(GCDH)was detected by ELISA,and the specificity,accuracy,precision,linear range,limit of quantitation(LOQ)and durability of the method were verified.Eight batches of rAAV9 samples were detected by the established method.Results The A_(450)-A_(630) value of the sample buffer was 0.3,which was slightly lower than the lowest dilution point(1 ng/mL)of the four-parameter standard curve for protein quantification.The average recoveries of samples with 150%,100% and 50% theoretical relative titer levels were in the range of 100.0%-107.3%.The RSDs of the target protein expression level of the samples with three theoretical relative titer levels detected by the same experimenter three times and different experimenters were all less than 25%.There was a good linear relationship between rAAV9 samples and the target protein expression levels in the range of 50%-150% theoretical relative titer levels,and the linear regression equation was y = 1.077 x-0.022,R~2= 0.984.The LOQ of the method was 0.59,namely 6.0×10~(12) vg/mL.After U87-MG cells were incubated with HU for different time(18,21,24 h),and the culture supernatant was stored under different conditions(room temperature for 0.5 h,below-60 ℃ for 12 h,below-60 ℃ for 24 h).The RSDs of target protein expression levels were all less than 25%.The target protein expression levels of 1-8 batches of rAAV9 samples were 111%,121%,72%,65%,86%,75%,102% and 91%,respectively.Conclusion The established method for the determination of the target protein expression level after rAAV infection has good specificity,accuracy,precision and durability,and can be used for the quality control of products in different stages of rAAV9 production.

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Objective To analyze the detection results of blood fat and proinsulin related indicators in the patients with type 2 diabetes mellitus(T2DM ) and to investigate the means for improving the patients′ clinical indicators .Methods 57 patients with T2DM in our hospital from February 2013 to February 2015 were divided into the research group ( ≥ 15 .6 mIU /L ,29 cases) and the control group (< 15 .6 mIU /L ,28 cases) according to the proinsulin level .All the cases took glucose ,at the same time the cor‐relation between the blood fat indexes with serum true insulin ,proinsulin and insulin resistance index of steady state insulin assess‐ment model was analyzed .Results Proinsulin ,serum true insulin ,2 h postprandial proinsulin ,2 h postprandial serum true insulin , insulin resistance index of steady state insulin assessment model all had correlation with and apolipoprotein B /apolipoprotein A1 . Conclusion The proinsulin level in the patients with T 2DM is increased ,thus the ratio of apolipoprotein B/apolipoprotein A1 will be accordingly increased .