Aims: Dermatophytosis is an emerging health problem in all age group people. The treatment is very challenging due to the high side effects of oral anti-fungal drugs and the increased resistance pattern against drugs and ointments. The aim of this study was to evaluate the sensitivity and resistance pattern of isolated and characterized dermatophytes to find the sensitive essential oil as a natural source of medicine to inhibit dermatophytosis Methodology and results: A total of 50 dermatophytes were isolated from different age groups and further sensitivity testing was performed against anti-fungal drugs, ointments, and essential oils. The best sensitivity was observed againstanti-fungal cream and three essential oils. Conclusion, significance and impact of study From 50 dermatophytes, the highest percentage of dermatophytes were obtained by the male patient under the age group of 19-64. In all, Trichophyton were the highly isolated species and showed high sensitivity against essential oil. Anti-fungal susceptibility testing is also vital for resistance surveillance and for comparing the in vitro activity of new and existing agents. The present study shows that natural essential oil can be a good alternative source of medication against these dermatophytes with no side effects.