1.Contralateral stroke with rapid recovery in a patient of herpes zoster ophthalmicus
Rajesh Verma ; Dilip Singh ; Pawan Sharma
Neurology Asia 2011;16(3):247-250
Herpes zoster ophthalmicus commonly presents with vesicular eruptions of skin along a dermatome
and post herpetic neuralgia. Viral invasion into the intracranial vessel and resultant arteritis is rare
complication, reported mainly in elderly, children and immunocompromised individuals. We report
a young immunocompetent male who reported to us with acute right sided hemiparesis following
an episode of left sided herpes zoster ophthalmicus three months prior to stroke. Patient had a near
complete recovery of his weakness without any treatment with persistent mild dysarthria. Magnetic
resonance imaging of brain showed subacute infarct in left middle cerebral artery territory. Magnetic
resonance angiogram demonstrated marked narrowing of the proximal segment of left middle cerebral
artery. This case highlights herpes zoster ophthalmicus as one of the causes of stroke in young with
good prognosis.
2.Influence of nitric oxide on in vitro growth, survival, steroidogenesis, and apoptosis of follicle stimulating hormone stimulated buffalo (Bubalus bubalis) preantral follicles.
Pawan K DUBEY ; Vrajesh TRIPATHI ; Ram Pratap SINGH ; G Taru SHARMA
Journal of Veterinary Science 2011;12(3):257-265
Effect of sodium nitroprusside (SNP), a nitric oxide (NO) donor, on in vitro survival, growth, steroidogenesis, and apoptosis of buffalo preantral follicles (PFs) was investigated. PFs (200~250 microm) were isolated by micro-dissection and cultured in 0 (control), 10(-3), 10(-5), 10(-7), and 10(-9) M SNP. To examine the reversible effect of SNP, PFs were cultured with 10(-5) M SNP + 1 mM Nomega-nitro-L-arginine methyl ester (L-NAME) or 1.0 microg hemoglobin (Hb). The results showed that greater concentrations of SNP (10(-3), 10(-5), 10(-7) M) inhibited (p < 0.05) FSH-induced survival, growth, antrum formation, estradiol production, and oocyte apoptosis in a dose-dependent manner. However, a lower dose of SNP (10(-9) M) significantly stimulated (p < 0.05) the survival, growth, antrum formation, follicular oocyte maturation, and stimulated progesterone secretion compared to the control. A combination of SNP + L-NAME promoted the inhibitor effect of SNP while a SNP + Hb combination reversed this effect. Nitrate and nitrite concentrations in the culture medium increased (p < 0.05) in a dose-dependent manner according to SNP concentration in the culture medium. At higher concentrations, SNP had a cytotoxic effect leading to follicular oocyte apoptosis whereas lower concentrations have stimulatory effects. In conclusion, NO exerts a dual effect on its development of buffalo PFs depending on the concentration in the culture medium.
Animals
;
*Apoptosis
;
Buffaloes/*physiology
;
Estradiol/biosynthesis
;
Female
;
Follicle Stimulating Hormone/metabolism
;
NG-Nitroarginine Methyl Ester/pharmacology
;
Nitrates/pharmacology
;
Nitric Oxide/*metabolism
;
Nitric Oxide Donors/pharmacology
;
Nitrites/pharmacology
;
Nitroprusside/pharmacology
;
Oocytes/cytology/drug effects/growth & development/metabolism
;
Ovarian Follicle/*cytology/drug effects/growth & development/*metabolism
;
Progesterone/biosynthesis
3.Blunt traumatic superior gluteal artery pseudoaneurysm presenting as gluteal hematoma without bony injury: A rare case report.
Annu BABU ; Amit GUPTA ; Pawan SHARMA ; Piyush RANJAN ; Atin KUMAR
Chinese Journal of Traumatology 2016;19(4):244-246
Blunt traumatic injuries to the superior gluteal artery are rare in clinic. A majority of injuries present as aneurysms following penetrating trauma, fracture pelvis or posterior dislocation of the hip joint. We reported a rare case of superior gluteal artery pseudoaneurysm following blunt trauma presenting as large expanding right gluteal hematoma without any bony injury. The gluteal hematoma was suspected clinically, confirmed by ultrasound and the arterial injury was diagnosed by CT angiography that revealed a large right gluteal hematoma with a focal contrast leakage forming a pseudoaneurysm within the hematoma. Pseudoaneurysm arose from the superior gluteal branch of right internal iliac artery, which was successfully angioembolized. The patient was discharged on day 4 of hospitalization with resolving gluteal hematoma. This report highlighted the importance of considering an arterial injury following blunt trauma to the buttocks with subsequent painful swelling. Acknowledgment of this rare injury pattern was necessary to facilitate rapid diagnosis and appropriate treatment.
Aneurysm, False
;
etiology
;
Buttocks
;
Hematoma
;
diagnostic imaging
;
etiology
;
Humans
;
Iliac Artery
;
injuries
;
Male
;
Middle Aged
;
Tomography, X-Ray Computed
;
Wounds, Nonpenetrating
;
complications
5.Cascade of Complications Following Carotid Body Tumor Excision
Pawan K GARG ; Sarbesh TIWARI ; Tushar S GHOSH ; Surendra PATEL ; Ankur SHARMA ; Pushpinder S KHERA
Neurointervention 2021;16(3):298-302
Carotid body tumor excision can lead to various complications including vascular injury and pseudoaneurysm formation. Here we describe a case of carotid body tumor excision followed by series of complications including pseudoaneurysm formation, failure of primary surgical repair, carotid stump syndrome following parent artery occlusion, and persistent hypotension.
6.Developmental Competence of Buffalo (Bubalus bubalis) Pluripotent Embryonic Stem Cells Over Different Homologous Feeder Layers and the Comparative Evaluation with Various Extracellular Matrices.
Manjinder SHARMA ; Pawan K DUBEY ; Rajesh KUMAR ; Amar NATH ; G Sai KUMAR ; G Taru SHARMA
International Journal of Stem Cells 2013;6(1):26-36
BACKGROUND AND OBJECTIVES: Use of somatic cells as a feeder layer to maintain the embryonic stem cells (ESCs) in undifferentiated state limits the stem cell research design, since experimental data may result from a combined ESCs and feeder cell response to various stimuli. Therefore, present study was designed to evaluate the developmental competence of the buffalo ESCs over different homogenous feeders and compare with various extracellular matrices using different concentrations of LIF. METHODS AND RESULTS: Inner cell masses (ICMs) of in vitro hatched blastocysts were cultured onto homologous feeders viz. fetal fibroblast, granulosa and oviductal cell feeder layers and synthetic matrices viz. fibronectin, collagen type I and matrigel in culture medium. Developmental efficiency was found higher for ESCs cultured on fetal fibroblast and granulosa layers (83.33%) followed by fibronectin (77.78%) at 30 ng LIF. Oviductal feeder was found to be the least efficient feeder showing only 11.11% undifferentiated primary ESC colonies at 30 ng LIF. However, neither feeder layer nor synthetic matrix could support the development of primary colonies at 10 ng LIF. Expression of SSEA-4, TRA-1-60 and Oct-4 were found positive in ESC colonies from all the feeders and synthetic matrices with 20 ng and 30 ng LIF. CONCLUSIONS: Fetal fibroblast and granulosa cell while, amongst synthetic matrices, fibronectin were found to be equally efficient to support the growth and maintenance of ESCs pluripotency with 30 ng LIF. This well-defined culture conditions may provide an animal model for culturing human embryonic stem cells in the xeno-free or feeder-free conditions for future clinical applications.
Animals
;
Blastocyst
;
Buffaloes
;
Collagen
;
Collagen Type I
;
Drug Combinations
;
Embryonic Stem Cells
;
Extracellular Matrix
;
Feeder Cells
;
Female
;
Fibroblasts
;
Fibronectins
;
Granulosa Cells
;
Humans
;
Laminin
;
Mental Competency
;
Models, Animal
;
Oviducts
;
Proteoglycans
;
Stage-Specific Embryonic Antigens
;
Stem Cell Research