1.Molecular Diagnostics for Detection of Microorganism.
Korean Journal of Clinical Microbiology 2003;6(1):1-6
No abstract available.
Pathology, Molecular*
3.Discussion on Key Control Points and Common Problems of Molecular Diagnostic Reagents in the Process of Design and Development.
Chinese Journal of Medical Instrumentation 2023;47(3):320-323
Design and development process of molecular diagnostic reagents is critical to quality management system of in vitro diagnostic reagent. Based on the technical characteristics of molecular diagnostic reagents, the study analyzed the concerned key control points and common problems in the process of design and development from the view of registration quality management system. It aimed at offering technical guidance on design and development process of molecular reagents and registration quality management system to enterprises, thus improving the product development efficiency, optimizing the quality management system, and increasing the efficiency and quality of registration and declaration.
Indicators and Reagents
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Pathology, Molecular
5.Methodological study on eliminating nucleic acid contamination in molecular diagnostic laboratory.
Yunlong LI ; Jian ZHANG ; Yanqiu WEI ; Xiaojuan JIA ; Xiaoyan LI ; Liming TAN ; Wenjun LIU ; Limin YANG
Chinese Journal of Biotechnology 2021;37(2):673-679
Nucleic acid detection technique has good sensitivity and specificity and is widely used in in vitro diagnosis, animal and plant commodity quarantine, forensic identification, and other fields. However, it is susceptible to carryover contamination during the operation and leads to false-positive results, which seriously affects the detection accuracy. Therefore, finding an effective solution to prevent and eliminate nucleic acid carryover contamination has become particularly urgent. This study compared several different methods for removing nucleic acid contamination and confirmed that sodium hypochlorite solution and PCRguard reagent could effectively eliminate nucleic acid carryover in the liquid and on surfaces of different materials. Besides, the combination of sodium hypochlorite solution and PCRguard can solve the nucleic acid aerosol contamination. This study proposes solutions for the routine prevention of carryover contamination and removal of aerosol that has occurred in molecular diagnostic laboratories.
Laboratories
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Nucleic Acids
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Pathology, Molecular
7.Application of Microfluidics in Molecular Diagnostics.
Chao WANG ; Dongfeng ZHANG ; Liuqing YANG ; Yaoji LIU ; Yue WU
Chinese Journal of Medical Instrumentation 2020;44(6):520-524
In recent years, molecular diagnostics has been the most promising branch of
Humans
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Microfluidics
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Molecular Diagnostic Techniques/instrumentation*
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Pathology, Molecular/instrumentation*
9.Evaluation of traditional pathological classification at molecular classification era for gastric cancer.
Chinese Journal of Gastrointestinal Surgery 2014;17(1):18-20
Histopathological classification is in a pivotal position in both basic research and clinical diagnosis and treatment of gastric cancer. Currently, there are different classification systems in basic science and clinical application. In medical literatures, different classifications are used including Lauren and WHO systems, which have confused many researchers. Lauren classification has been proposed for half a century, but is still used worldwide. It shows many advantages of simple, easy handling with prognostic significance. The WHO classification scheme is better than Lauren classification in that it is continuously being revised according to the progress of gastric cancer, and is always used in the clinical and pathological diagnosis of common scenarios. Along with the progression of genomics, transcriptomics, proteomics, metabolomics researches, molecular classification of gastric cancer becomes the current hot topics. The traditional therapeutic approach based on phenotypic characteristics of gastric cancer will most likely be replaced with a gene variation mode. The gene-targeted therapy against the same molecular variation seems more reasonable than traditional chemical treatment based on the same morphological change.
Humans
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Molecular Biology
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Stomach Neoplasms
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classification
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pathology
10.Applications of molecular imaging in vulnerable atherosclerotic plaque.
Acta Academiae Medicinae Sinicae 2009;31(2):248-252
Atherosclerosis plaque is the underline cause of ischemic stroke and acute coronary syndrome. In this article, the applications of molecular imaging in vulnerable plaque are reviewed.
Atherosclerosis
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diagnosis
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pathology
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Humans
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Molecular Imaging
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methods