Eight rabbits exhibited head tilt and subsequently died. At necropsy, three rabbits had crusty deposits in ears and four had reddish lungs. The main histopathological features were severe diffuse suppurative meningoencephalitis (75.0% of rabbits), fibrinopurulent pneumonia (37.5%), and otitis externa (37.5%). Pasteurella multocida (P. multocida) was isolated from brains, ears, and lungs. The capsular serogroups of the isolates were untypable. Based on histopathological features and bacterial analysis results, the rabbits were diagnosed as P. multocida infection. P. multocida infections might result in considerable economic loss in commercial rabbit production facilities in Korea.
Brain ; Ear ; Head ; Korea ; Lung ; Meningoencephalitis ; Otitis Externa ; Pasteurella Infections ; Pasteurella multocida ; Pasteurella ; Pneumonia ; Rabbits ; Serogroup

Pasteurella multocida infection in man is rare. This organism was isolated from a calf infection of a 48-year-old woman with degenerative arthritis who had the history of treatments with prednisolone, acupuncture and moxa cauterization. She did not have any animal contact. It was condsidered probable that the organism invaded through the cauterization ulcers. The organism was difficult to identify, because of its superficial resemblance to other organisms. Oxi/Ferm and N/F systems failed to identify the isolate. The organism was susceptible to many antimicrobial agents tested except to amikacin and clindamycin.
Female ; Human ; Leg Ulcer/etiology* ; Middle Age ; Moxibustion/adverse effects* ; Osteoarthritis/therapy* ; Pasteurella Infections/etiology*

Pasteurella dagmatis is an oxidase and catalase positive, facultative anaerobic, gram-negative coccobacillus classified as a member of the family Pasteurellaceae. Pasteurella species are commonly colonizing the oropharynx of healthy domestic and wild animals including cats and dogs. These are usually pathogenic to domestic animals, but rarely to human beings. Pasteurella infection of human causes pneumonia, empyema, meningitis, peritonitis, bone and joint infection and septicemia. Recently, we experienced a case of dog-bite wounds from which Pasteurella dagmatis was isolated in a 39-year-old woman. To our knowledge, this is the first report of Pasteurella dagmatis isolated from dog-bite wounds in Korea.
Adult ; Animals ; Animals, Domestic ; Animals, Wild ; Catalase ; Cats ; Colon ; Dogs ; Empyema ; Female ; Humans ; Joints ; Korea ; Meningitis ; Oropharynx ; Oxidoreductases ; Pasteurella Infections ; Pasteurella* ; Pasteurellaceae ; Peritonitis ; Pneumonia ; Sepsis ; Wounds and Injuries*

Pasteurellae are aerobic gram-negative coccobacillary organism and usually pathogenic to domestic animal, but rarely to human beings. Human Pasteurella infections are mostly caused by animal but occasionally happens without known history of animal contacts. Pasteurella infection of human has been reported in numerous systems including pneumonia, empyema, meningitis, peritonitis, bone and joint infection and septicemia. This organism was difficult to identify because of its superficial resemblance to other organism, until recently a new technique used a requirement of X, V factor is developed. This organism is susceptible to many antimicrobial agents. A 55-year-old man was admitted to Seoul Paik Hospital Inje University due to fever and pleuritic chest pain for 2 weeks. He denied any animal contact history. Pasteurella hemolytica was recovered from pleural effusion. This is the first case report of empyema caused by Pasteurella hemolytica in Korea and provides another definitive causative organism of empyema.
Animals ; Animals, Domestic ; Anti-Infective Agents ; Chest Pain ; Empyema* ; Fever ; Fibrinogen ; Humans ; Joints ; Korea ; Mannheimia haemolytica* ; Meningitis ; Middle Aged ; Pasteurella Infections ; Pasteurella* ; Peritonitis ; Pleural Effusion ; Pneumonia ; Seoul ; Sepsis

Bite wounds are often mistakenly considered innocuous. However, they are frequently complicated by infection which may be serious. We describe a case of Pasteurella multocida septicaemia with myopericarditis following a dog bite. Treatment of the infection as well as active support of myocardial function led to a successful outcome.
Adult ; Animals ; Bites and Stings ; microbiology ; Dogs ; Hand Injuries ; microbiology ; Humans ; Male ; Pasteurella Infections ; pathology ; Pasteurella multocida ; Pericardial Effusion ; microbiology ; Pericarditis ; microbiology ; Sepsis ; pathology ; Wound Infection ; pathology

The immunologic reactivity of a lipopolysaccharide (LPS)-protein complex isolated from a potassium thiocyanate extract of a Pasteurella multocida (capsular type A and somatic type 3) strain was evaluated in mice. The LPS-protein complex provided 100% protection in mice against a challenge with the homologous strain. However, when the complex was fractionated into LPS and protein moieties by phenol-water treatment, both components lacked immunogenicity. The complex and extracted components were mitogenic for mouse B lymphocytes with the protein moiety the most active. Although immune serum against the LPS-protein complex protected mice against challenge thereby indicating a role for humoral immunity, the LPS-protein complex of P. multocida was also found to induce cell-mediated immunity. This cell-mediated immunity was demonstrated in mice immunized with the complex by: (1). mitogenic responses of T lymphocytes, (2). induction of delayed type hypersensitivity reaction in the hind footpads, and (3). enhanced resistance to challenge infection with Salmonella enteritidis.
Animals ; Antibodies, Bacterial/blood/immunology ; Bacterial Proteins/chemistry/*immunology ; Chemical Fractionation ; Hypersensitivity, Delayed ; Immune Sera/immunology ; Immunity, Cellular ; Immunization, Passive ; Lipopolysaccharides/chemistry/*immunology ; Lymphocyte Activation ; Mice ; Pasteurella Infections/immunology/*prevention & control ; Pasteurella multocida/*chemistry/immunology ; Salmonella Infections, Animal/immunology/prevention & control ; Salmonella enteritidis/growth & development/immunology ; Spleen/cytology/immunology/microbiology

No abstract available.
Animals ; Anti-Bacterial Agents/pharmacology ; Bites and Stings ; Disk Diffusion Antimicrobial Tests ; Dogs ; Female ; Humans ; Middle Aged ; Pasteurella/drug effects/genetics/*isolation & purification ; Pasteurella Infections/*diagnosis/microbiology ; RNA, Ribosomal, 16S/chemistry/genetics/metabolism ; Republic of Korea ; Sequence Analysis, DNA ; Soft Tissue Infections/*diagnosis/microbiology

The antigenicity of Photobacterium damselae (Ph. d.)subsp. piscicida, cultured in four different growth media[tryptone soya broth (TSB), glucose-rich medium (GRM),iron-depleted TSB (TSB+IR-), and iron-depleted GRM(GRM+IR-)] was compared by enzyme-linked immuno-sorbent assay (ELISA) and Western blot analysis usingsera obtained from sea bass (Dicentrarchus labrax) raisedagainst live or heat-killed Ph. d. subsp. piscicida. Theantigenic expression of Ph. d. subsp. piscicida was found todiffer depending on the culture medium used. A significantlyhigher antibody response was obtained with iron-depletedbacteria by ELISA compared with non-iron depletedbacteria obtained from the sera of sea bass raised againstlive Ph. d. subsp. piscicida. The sera from sea bass raisedagainst live bacteria showed a band at 22kDa in bacteriacultured in TSB+IR- or GRM+IR- when bacteria thathad been freshly isolated from fish were used for thescreening, while bands at 24 and 47kDa were observedwith bacteria cultured in TSB or GRM. When bacteriawere passaged several times on tryptic soya agar prior toculturing in the four different media, only bands at 24 and47kDa were recognized, regardless of the medium used toculture the bacteria. It would appear that the molecularweight of Ph. d. subsp. piscicida antigens change in thepresence of iron restriction, and sera from sea bassinfected with live bacteria are able to detect epitopes onthe antigens after this shift in molecular weight.
Animals ; Antibodies, Bacterial/blood ; Antigens, Bacterial/immunology/*metabolism ; Bass/blood/*immunology ; Blotting, Western/veterinary ; Cell Count/methods ; Culture Media ; Enzyme-Linked Immunosorbent Assay/veterinary ; Fish Diseases/immunology/*microbiology ; Molecular Weight ; Pasteurella Infections/immunology/microbiology/*veterinary ; Photobacterium/*immunology

Epidemiological characteristics of swine pulmonary Pneumocystis (P.) carinii and concurrent infections were surveyed on Jeju Island, Korea, within a designated period in 172 pigs submitted from 54 farms to the Department of Veterinary Medicine, Jeju National University. The submitted cases were evaluated by histopathology, immunohistochemistry, PCR/RT-PCR, and bacteriology. P. carinii infection was confirmed in 39 (22.7%) of the 172 pigs. Histopathologically, the lungs had moderate to severe lymphohistioctyic interstitial pneumonia with variable numbers of fungal organisms within lesions. Furthermore, porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV-2) co-infection was a common phenomenon (12.8%, 20.5%, and 48.7% were positive for PRRS, PCV-2, or both, respectively, as determined by PCR/RT-PCR). Infection was much more concentrated during winter (December to March) and 53.8% of the infected pigs were 7- to 8-weeks old. In addition, three pigs showed co-infection with bacteria such as Pasteurella multocida and Streptococcus suis. The results of the present study suggest that the secondary P. carinii infection is common following primary viral infection in swine in Korea. They further suggest that co-infection of P. carinii might be enhanced by the virulence of primary pathogens or might have synergistic effects in the pigs with chronic wasting diseases.
Aging ; Animals ; *Circovirus/pathogenicity ; Incidence ; Pasteurella Infections/complications/epidemiology/veterinary ; Pasteurella multocida/i ; *Pneumocystis carinii/immunology/pathogenicity ; Pneumonia, Pneumocystis/complications/epidemiology/physiopathology/*veterinary ; Porcine Postweaning Multisystemic Wasting Syndrome/complications/*epidemiology ; Porcine Reproductive and Respiratory Syndrome/*epidemiology ; *Porcine respiratory and reproductive syndrome virus/pathogenicity ; Prevalence ; Republic of Korea/epidemiology ; Reverse Transcriptase Polymerase Chain Reaction ; Sea ; Streptococcal Infections/complications/epidemiology/veterinary ; Streptococcus suis/i ; Sus scrofa ; Swine Diseases/epidemiology/virology

At the Department of Communicabe Disease of Ha Noi – Dong Da Hospital 546 patients (52 dengue fever (DF) patients and 44 dengue hemorrhagic fever (DHF) patients were studied. IgM antibody was quantified by ELISA, blood count by 680 plus device. Results: a leucopenia (<4000) 24.2% in DF and 15.9% in DHF. Thrombocytopenia 51.2% in DF. In DF, 81.8% exerted an antibody response; in DHF there were a high rate of stimulating lympho cells (84.1%), there is 100% correlation between stimulating lympho cell and IgM antibody response
Dengue ; Hemorrhagic Septicemia, Viral ; Diseases