1.Human Bocavirus in Patients with Respiratory Tract Infection.
Jang Su KIM ; Chae Seung LIM ; Young Kee KIM ; Kap No LEE ; Chang Kyu LEE
The Korean Journal of Laboratory Medicine 2011;31(3):179-184
BACKGROUND: Human bocavirus (HBoV) is a newly identified viral pathogen, and its clinical epidemiology and significance in respiratory infections have not yet been completely elucidated. We investigated the prevalence of HBoV infection and the association between viral (HBoV) load and clinical features of the infection in patients of all age-groups. METHODS: Nasopharyngeal aspirates from patients with symptoms of respiratory infection were tested for presence of HBoV by using real-time polymerase chain reaction. HBoV-positive patients were categorized into low- and high-viral-load groups using 1.0x10(6) copies/mL as the threshold value of viral load. RESULTS: Detection rate of HBoV was 4.8% (N=93) in a total of 1,926 samples with peak incidence of infection being observed in patients aged 6-12 months. HBoV infection was more frequently observed in young children, especially, in children aged less than 5 yr, and the HBoV load decreased with increase in age. HBoV was codetected with other respiratory viruses in 17 (18.3%) of the 93 HBoV-positive patients and 15 patients (88.2%) belonged to the low-viral-load group. Patients infected with HBoV alone showed a higher viral load than those patients in whom HBoV was codetected with other respiratory viruses (median load, 3.78x10(5) copies/mL vs. 1.94x10(4) copies/mL, P=0.014). Higher pulse rate (P=0.007) and respiratory rate (P=0.021) were observed in patients with a high-viral-load. CONCLUSIONS: Our results suggest that HBoV may be the causative agent of respiratory infection in the high-viral-load group.
Adolescent
;
Adult
;
Aged
;
Child
;
Child, Preschool
;
DNA, Viral/analysis
;
Female
;
Human bocavirus/*isolation & purification
;
Humans
;
Infant
;
Male
;
Middle Aged
;
Nasopharynx/virology
;
Parvoviridae Infections/diagnosis/*epidemiology/virology
;
Polymerase Chain Reaction
;
Prevalence
;
Respiratory Tract Infections/diagnosis/*epidemiology/virology
;
Viral Load
2.Human Bocavirus in Patients with Respiratory Tract Infection.
Jang Su KIM ; Chae Seung LIM ; Young Kee KIM ; Kap No LEE ; Chang Kyu LEE
The Korean Journal of Laboratory Medicine 2011;31(3):179-184
BACKGROUND: Human bocavirus (HBoV) is a newly identified viral pathogen, and its clinical epidemiology and significance in respiratory infections have not yet been completely elucidated. We investigated the prevalence of HBoV infection and the association between viral (HBoV) load and clinical features of the infection in patients of all age-groups. METHODS: Nasopharyngeal aspirates from patients with symptoms of respiratory infection were tested for presence of HBoV by using real-time polymerase chain reaction. HBoV-positive patients were categorized into low- and high-viral-load groups using 1.0x10(6) copies/mL as the threshold value of viral load. RESULTS: Detection rate of HBoV was 4.8% (N=93) in a total of 1,926 samples with peak incidence of infection being observed in patients aged 6-12 months. HBoV infection was more frequently observed in young children, especially, in children aged less than 5 yr, and the HBoV load decreased with increase in age. HBoV was codetected with other respiratory viruses in 17 (18.3%) of the 93 HBoV-positive patients and 15 patients (88.2%) belonged to the low-viral-load group. Patients infected with HBoV alone showed a higher viral load than those patients in whom HBoV was codetected with other respiratory viruses (median load, 3.78x10(5) copies/mL vs. 1.94x10(4) copies/mL, P=0.014). Higher pulse rate (P=0.007) and respiratory rate (P=0.021) were observed in patients with a high-viral-load. CONCLUSIONS: Our results suggest that HBoV may be the causative agent of respiratory infection in the high-viral-load group.
Adolescent
;
Adult
;
Aged
;
Child
;
Child, Preschool
;
DNA, Viral/analysis
;
Female
;
Human bocavirus/*isolation & purification
;
Humans
;
Infant
;
Male
;
Middle Aged
;
Nasopharynx/virology
;
Parvoviridae Infections/diagnosis/*epidemiology/virology
;
Polymerase Chain Reaction
;
Prevalence
;
Respiratory Tract Infections/diagnosis/*epidemiology/virology
;
Viral Load
3.Prevalence of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2 and porcine parvovirus from aborted fetuses and pigs with respiratory problems in Korea.
Kwang Soo LYOO ; Yong Ho PARK ; Bong Kyun PARK
Journal of Veterinary Science 2001;2(3):201-207
Porcine reproductive and respiratory syndrome virus(PRRSV)0, porcine circovirus type 2(PCV-2) and porcine parvovirus (PPV)0 infections were investigated as possible causes of the postweaning multisystemic wasting syndrome(PMWS). Specific primers for RT-PCR and PCR were designed for the differential detection of PRRSV, PCV-2 and PPV. Using PCR, these viruses were detected in homogenized tissue samples from pigs that had respiratory of reproductive problems in the time period between 1998 and 2000; the overall prevalences were: PRRSV 31.4%, PCV-2 46.5%, and PPV 8.1%. PCV-2 was also detected in aborted fetal tissues.
Aborted Fetus/virology
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Animals
;
Base Sequence
;
Circoviridae Infections/diagnosis/epidemiology/*veterinary
;
Circovirus/genetics/isolation&purification
;
DNA Primers
;
Diagnosis, Differential
;
Korea/epidemiology
;
Parvoviridae Infections/diagnosis/epidemiology/*veterinary
;
Parvovirus, Porcine/genetics/isolation&purification
;
Polymerase Chain Reaction/methods/veterinary
;
Porcine Reproductive and Respiratory Syndrome/diagnosis/*epidemiology
;
Porcine respiratory and reproductive syndrome virus/genetics/isolation & purification
;
Prevalence
;
Respiratory Tract Infections/veterinary/virology
;
Reverse Transcriptase Polymerase Chain Reaction/methods/veterinary
;
Sequence Homology
;
Swine
;
Swine Diseases/diagnosis/*epidemiology
;
Wasting Syndrome/*veterinary/virology