Poisoned the rats by pesticide wofatox with dose 10mg/kg of their body weight. After 60 minutes, cut off two third of liver by method of Higgins and Anderson. The changes in morphological structures and mitotic activity of liver rats were as follows: Lesions in the liver were markedly manifested, but were not prolong. The mitotic activity of hematocytes decreased. The regenenation of liver weakly manifested
Methyl Parathion ; Rats ; Liver

Study on the changes of ultra and micro-structure of the liver of white rats, which was effected by Wafatox with longed-low dose. Rats with the beginning body mass of 120-150g were everyday oral administrated by oil containing 0.1% of Wafatox with the dose of 0.5mg/kg of their body mass (similarly with 1/70 dose of LD50 for white rats). They were killed by stages of 10, 20, 30, 40 and 50 days from the first oral administration. The results: in clinical, the rat’s intoxicated symptoms were not clear, but the changes in the liver were clearly shown. In the liver, there were some points of necrozism, hepatocytes strongly regressed at the level of ultra and micro-structure. The portals areas and the liver’s tissues were infiltrated by cells like lymphocytes. There were happened stretching veins, stagnating blood and particularly, getting thicker and thicker in the walls of veins of central lobules of the rats’ liver at every stage. Up to the 50th day of intoxication by Wafatox, the quantity of two-nucleus hepatocytes was decreased and mitotic activity of hepatocytes was not increased
Methyl Parathion ; Rats ; Liver

Chromatography, Gas ; methods ; Dimethoate ; blood ; Humans ; Insecticides ; blood ; Methyl Parathion ; blood

The evaluation of the bioavailability of pollutants in soil is crucial to accurately assess the pollution risk, and whole-cell biosensor is one of the important tools for such evaluation. This study aimed to develop a novel whole-cell biosensor for the detection of methyl parathion in soil using. First, a whole-cell biosensor was constructed by the screened methyl parathion hydrolase mpd gene, the existing specific induction element pobR, and the pUC19 plasmid skeleton. Then, the detection method of methyl parathion in soil extracts was established using 96-well microtiter plate as carrier and five whole-cell biosensors as indicator. The method was applied in the detection of methyl parathion in tested and field soil extracts. Taking E. coli DH5α/pMP-AmilCP with the best detection performance as an example, this biosensor had a detection limit of 6.21-6.66 µg/L and a linear range of 10-10 000 µg/L for methyl parathion in four soil extracts. E. coli DH5α/pMP-RFP and E. coli DH5α/pMP-AmilCP methods have good detection performance for the analysis of methyl parathion in soil extract samples. This biosensor method can help to quickly assess the bioavailability of methyl parathion in soil, and thus help to understand the risk of soil pollution caused by organophosphorus pesticide methyl parathion.
Methyl Parathion/analysis* ; Pesticides/analysis* ; Organophosphorus Compounds ; Escherichia coli/genetics* ; Soil ; Farms ; Biosensing Techniques

Animals ; Chromatography, Thin Layer ; Dichlorvos ; blood ; Dimethoate ; blood ; Insecticides ; blood ; Methyl Parathion ; blood ; Mice ; Organothiophosphorus Compounds ; blood

We observed the change of serum cholinesterase and clinical symptoms of acute parathion intoxication in I.C.U. of N.M.C. from 1975 Sep. to 1976 Oct. The results were as follows; 1. Serum choinesterase level of control group (55 cases) was l00. 53+/-13. 8 and range was 74~125 unit. 2. Serum cholinesterase level were reduced markedly below 10 units in 10 cases of severe parathion intoxication. 3. With adequate treatment PAM & atropine, serum cholinesteraee level rose steadly from 3 rd hospital day to 7 th hospital day and thereafter marked rising was noted remarkable improvement of respiratory symptoms. 4. In cases of low serum cholinestrsse level, sudden aggravation of general symptoms even death occurred and the symptoms & signs were correlated serum cholinesterase level.
Atropine ; Cholinesterases* ; Clinical Study* ; Parathion*

The Authors observed 16 cases of Parathion intoxication from 1972 to 1974 in LC.U. of Busan National University Hospital. The results were as follows; 1. Parathion solution (20%) was taken for the purpose of suicide in all 16 cases. 2. Male-to-female ratio was 1. 28: 1. Age distribution ranged from 18 to 31, and the highest age group was 2 nd decade (ll cases), next 3 rd (3 cases), and 1st decade (2 cases) in order. 3. Culture of bronchial secretion was done in 6 cases and Pseudomonas Aeruginosa were detected in 5 cases. 4. Adequate control of respiratory problems and drug therapies for this intoxication resulted.
Age Distribution ; Busan ; Drug Therapy ; Humans ; Parathion* ; Pseudomonas aeruginosa ; Suicide

A case of parathion poisoning has been reported; in which the symptoms of hypoxia resulted from partial airway obstruction, however this was mistaken for parathion poisoning, and the correct diagnosis was proved by removal of hard blood clot in the bronchial bifurcation. Conclusions were as follows: Adquate administration of PAM and atropine presented impressive response of symptoms. 2. Careful and frequent removal of secretion might be the most important problem especially in this poisoning. 3. Massive administration of antibiotics could prevent pulmonary complications. 4. Pulmonary edema, the most common complication, was not present.
Airway Obstruction* ; Anoxia ; Anti-Bacterial Agents ; Atropine ; Diagnosis ; Paralysis* ; Parathion* ; Poisoning* ; Pulmonary Edema

The aquatic quality of the Naktong river after two of three months in June, 1991 with phenol spillage from a electrical factory in Kumi was investigated. The samples were collected at six sites of the Naktong river basin and Kachang and Kongsan lakes. Phenol was not detected from all water samples. Turbidity was very much increased to the down stream in the Naktong river. The BOD and COD values exceeded the 2nd grade(3 mg/l) of the Korean standard quality of Environmental Water Act at the all sampling sites of the Naktong river. Especially, the value of COD at Kaejin (12.5 mg/l) was poorly classified as to the 5th grade of water class for the environmental quality standards. Organophosphorous pesticides such as parathion, malathion, fenitrothion and diazinon were investigated but not detected. Diazinon was only detected at the Ilson bridge(1.42 ppb), Okkye stream(6.95 ppb), Waekwan bridge(0.32 ppb), Gangjung reservior(0.13 ppb), Kaejin(0.05 ppb). Of the carbamates such as carbanyl, isoprocarb and cabofuran, the carbofuran was detected all sites except tap water, and Kachang and Kongsan lakes. The content of heavy metals such ans Cd, Pb, Zn, Fe, Mn, Hg were not exceeding for drinking water standards at the all sampling region, but only mecury was detected from Okkye stream(0.018ppb) and Kaejin(0.09ppb). In the regions of Kachang and Kongsan lakes, the content of heavy metals were lower than that of reservoir of Naktong river.
Carbamates ; Carbofuran ; Diazinon ; Drinking Water ; Fenitrothion ; Gyeongsangbuk-do* ; Korea* ; Lakes ; Malathion ; Metals, Heavy ; Parathion ; Pesticides ; Phenol* ; Rivers* ; Water ; Water Quality

Clonorchiasis for which no specific therapy is available has been recognized as one of the serious public health problems in terms of waste of human productivity in Korea today and an urgent consideration must be given to its prevention and control. For past several years, the author has been finding that the infection rate of liver fluke in fish from the Kum-Ho river which has long been recognized as the world's notorious prevalent area of the disease, has been decreasing gradually. Here the author formulated the hypothesis that the decreasing of infection rate might be influenced by the common use of pesticide in plants along the river. Basing this hypothesis, the effective use of pesticide may contribute to the control of the disease by cutting the life cycle of liver fluke. To test above hypothesis, molluscicidal effect on Parafossarulus manchouricus and cercariacidal effect of various pesticides in liver fluke have been determined and the following results were obtained. In molluscicidal effect, the most potent agent in low concentration was Bayer 73 which is 3 to 70 times stronger than other pesticides. When compared with NaPCP which has been widely used for the eradication of Onchomelania, Bayer 73 shows 5 to 10 times stronger effect. The order of pesticide in molluscicidal effect was: Bayer 73, Nicotin, NaPCP, Sevin, Parathion, Copper sulfate and Lindane. In cercariacidal effect, the most potent agent was Parathion which had 2 to 5 times stronger effect than Bayer 73 or NaPCP. The order of pesticide in the cercariacidal effect was: Parathion, Bayer73, NaPCP, Lindane, Nicotin,Sevin, and Copper sulfate. By applying the common used concentration of pesticides considering the toxicity to plants, Bayer 73 is most potent in molluscicidal effect and then Nicotin, NaPCP, Copper sulfate, Sevin, Parathion and Lindane in order. In cercariacidal effect, Parathion is the most potent and NaPCP, Bayer 73, Lindane, and Copper sulfate are followed. Considering with the toxicity to human body the most safe and effective pesticides were Bayer 73, Seven, NaPCP, and Copper sulfate but among those agents, Bayer 73 and NaPCP show relatively stronger effect in both Parafossarulus manchouricus and cercaria of liver fluke.
parasitology ; helminth ; trematode ; Clonorhcis sinensis ; chemotherapy ; Parafossarulus manchouricus ; Onchomelania ; cercaria ; toxicology ; Bayer 73 ; Nicotin ; NaPCP ; Sevin ; Parathion ; Copper sulfate ; Lindane