The survival ability of the larvae of Clonorchis sinensis in raw fish dish prepared by traditional culinary method in Nghia Lac commune, Nghia Hung District, Nam Dinh province and the larvae of Paragonimus heterotrimus in grilled crabs processed in Khanh Hoa commune, Luc Yen district, Yen Bai province was investigated. In this raw fish dish, 93-95% of the microcerca of Clonorchis sinensis and in this grilled crab, 23.3-65% of microcerca had been living
Clonorchis sinensis ; Fishes ; Paragonimus

No abstract available.
Paragonimiasis ; Paragonimus westermani ; Stroke

This survey was undertaken to determine the recent prevalence of lung fluke, Paragonimus westermani, in the three localities of Wiseong county, Kyungpook Province, Korea and to compare the prevalence among the residents in the same areas between 1924 and 1980. The three localities, Ku-seong, Sam-chun, and Um-ji villages (dong) have been known as the endemic areas of Paragonimus westermani. The surveys were made on the general population. They were examined using intradermal test with Paragonimus westermani antigen, and the diagnosis of paragonimiasis was made depending on the positive intradermal reaction. The sputum examination could not be performed beacause the residents were inaccessible. The prevalence of Paragonimus westermani among the residents in the three communities varied from 1.4 percent to 7.9 per cent, with an average of 4.0 per cent. Whereas, the average reported by Ichinomiya in 1924 was 4.7 percent. There are no significant differences in the sex and age specific rates of infection between 1924 and 1980. With the abundant density of Semisulcospira snails and crayfish in Sam-chun stream and the high rate of infection with Paragonimus westermani among the residents in the village, it is found that Sam-chun village, one of the three localitied in Wiseong county, where Ichinomiya (1924) surveyed, was still the endemic focus of Paragonimus westermani.
parasitology-helminth-trematoda ; Paragonimus westermani ; paragonimiasis ; epidemiology

By molecular identification and using mitochondrial genetic markers, the results showed that different forms of lung fluke in Vietnam, including adult fluke from human, dogs, cats and Potamicus sp. rock crab, have been identified as Paragonimus heteotremus. Molecular-based analysis on 390 nucleotides of the cytochrome oxidase gene revealed that Paragonimus heterotremus of Vietnam from all forms showed high identity to the Chinese and Thai strains. (99.0 -99.2% nucleotide and 97-100% amino acid). Phylogenetic analysis uniquely placed the Vietnamese Paragonimus sp to the group of P.heterotremus of Chinese and Thai origin. Thus, P.heterotremus is offically identified from most of the natural and experimental hosts in Vietnam
Molecular Diagnostic Techniques ; Paragonimus ; Genome, Mitochondrial

Metacercariae were found from 6 samples among 4,382 Parlemon nipponensis which were caught at Podu, Kohung, Korea. The metacercaria was inclined as a larval stage of Paragonimus westermani by following reason: Semisulcospira libertina was the only one species which lives in Podu area. No other metacercaria except Paragonimus westermani were found from the craw or cray fish which were caught in the endemic aera. Metacercariae, ovary, testes and cuticular spines resmbled the characteristics of Paragonimus westermani, which have been described by previous workers.
parasitology ; helminth ; trematoda ; Paragonimus westermani ; metacercaria ; epidemiology

The present study was undertaken to demonstrate the surface structure of Paragonimus westermani metacercaria in Korea with special reference to the distribution of sensory papillae. Metacercariae were isolated from crayfish, one of the second intermediate host of P. westermani in Bogil island, Chollanam-do (Province), Korea, where has been known as an endemic area of human paragonimiasis. Isolated metacercariae were excysted and examined with light, scanning and transmission electron microscopes for morphological features. On the surface of metacercariae, three types of sensory papillae were identified. Large domed papillae (3-5 micrometer), which were covered with wrinkled plasma membrane of the worm, were distributed on the oral and ventral suckers only. On the oral sucker, these large domed papillae were 12-13 in number. On the other hand large domed papillae on the ventral sucker were constantly 6 in number and hexagonal in distribution. Small domed papillae (2-3 micrometer), of which surface was more smooth than those of large ones, were distributed symmetrically on the ventral (30-32 pairs) and dorsal surfaces (40-42 pairs). Ciliated papillae (0.8-1.5 micrometer) were observed about 5-6 in number around the oral sucker and 3-5 pairs each on the ventral and dorsal surface of the body. Single pointed spines covered the entire surface of the body except around the excretory pore. Spines on the anterior part of the body were 0.9-2.0 micrometer in length and 45-55/100 square micrometer in number, and were gradually reduced in length (0.4-1.4 micrometer) and in number (12-27/100 square micrometer) toward the posterior part. The body wall of P. westermani metacercariae was consisted with anucleated syncytium layer, fibrous interstitial layer and musclar layer. In the anucleated syncytium, biconcave (0.15-0.55 micrometer) and spherical (0.08-0.16 micrometer) secretory granules, which were transferred from epidermal cells via protoplasmic tubules, mitochondria and ribosomes, were observed. Spines originated around the basement membrane protruded externally. Epidermal cells were consisted with a nucleus and a cytoplasm, and connected to syncytium with protoplasmic tubules. In the cytoplasm many secretory granules, mitochondria, Golgi complex, endoplasmic reticula, ribosomes and lipid droplets were observed.
parasitolgy-helminth-trematoda ; Paragonimus westermani ; electron microscopy

The present study was performed to observe the ultrastructure of the integument of adult Paragonimus westermani. Dogs experimentally infected with 60 metacercariae of P. westermani were autopsied 4 months after the infection. Adult P. westermani were extracted from the dogs and the fine structure was studied by means of scanning and transmission electron microscope. The findings are as follows: Scanning electron microscopic findings showed that the spines and the papillae are distributed at whole body surface but the well developed spines or papaillae are shown around the oral suckrer and ventral sucker. At the end of the body, excretory pore was found, the shape was irregular. Transmission electron microscopic findings showed that plasma membrane, tegument, basal lamina, connective tissue, circular muscle layer, longitudinal muscle layer, nerve axon and tegumental cell were observed. In higher magnification, plasma membrane and bar-shaped granules were found at the outer surface of the tegument.
parasitology-helminth-trematoda ; Paragonimus westermani ; electron microscopy

An epidemiological study of clonorchiasis and paragonimiasis westermani prevailed among the people of a rural county. The author studied the infection rates of clonorchiasis sinensis and paragonimiasis westermani in the ingabitants of Chungsong Gun, Kyungbuk Province, Korea. The examinations were carried out from March 1965 to November 1965 fowards 1.303 inhabitants whose ages were ranging from three months to over sixty years. All the inhabitants were tested intradermally with clonorchis and paragonimus antigen and then confirmed the eggs by M.G.L. technique and sputum test. The results of the examination were summarized as follows : 1. Out of the persons tested, 15.8% were infected with clonorchiasis sinensis. 2. Out of the persons tested with paragonimus antigen, 20.9% were infected with Paragonimus westermani. 3. The infection rate of paragonimiasis was higher than that of clonorchasis, 4. The infection rate of clonorchiasis in male was 20.8%(147 out of 706), while that in female was 7.1%(58 out of 597). The infection rate of paragonimiasis in male was 21.2%(150 out of 706), while that in female was 20.1%(120 out of 597). 5. The maximum infection rates of clonorchiasis and paragonimiasis were observed among the 45-49 age group(65.2% and 43.5%) respectively, however it was gradually decreased in the senile group. 6. By occupation, the maximum infection rate of clonorchisasis was observed in policemen(45.7%) and the maximum carrier of eggs(by sputum test) was observed in unemployed(paragonimiasis). 7. By education, the maximum infection rate of clonorchiasis was observed in the unemployed(28.9%) and the maximum carrier of eggs(by sputum test) was observed in the unemployed(paragnomiasis).
Clonorchiasis* ; Education ; Eggs ; Epidemiologic Studies* ; Female ; Humans ; Korea ; Male ; Occupations ; Ovum ; Paragonimiasis* ; Paragonimus ; Paragonimus westermani ; Sputum

The new drug niclofolan (bayer 9015, Bilevon), 5,5'-dichloro-2,2'-dihydroxy-3, 3'-dinitrobiphenyl, has been used in the treatment of Paragonimus westermani in dogs and cats and P. iloktsuenensis in rats. It was proved that administration of daily dose of 1.0 mg/kg body weight for 3 days or in 2 doses of 2.0 mg/kg body weight by alternate days were evidently effective for the infected dogs, cats and rats with the lung flukes, and toxic manifestations were not found.
parasitology-helminth-trematoda ; Paragonimus iloktsuenensis ; paragonimiasis ; Paragonimus westermani ; dog ; cat ; rat ; niclofolan-chemotherapy ; niclofolan

This study was undertaken to evaluate the status of human paragonimiasis in Kang Hwa Gun, Kyunggi Do, Korea from March 1973 to October 1975 by the use of intradermal screening test with Veronal buffered saline antigen. A total number of 7,352 (male 3,936; female 3,4l6) consisting of 3,447 primary school children, 2,689 middle school students, l,008 high school students of 9 Myuns out of 13 Myuns of this County and 208 residents of all age group in Kilgeong-Ri, Yangdo Myun were examined by the intradermal test with Paragonimus antigen. In order to detect Paragonimus egg, the sputum and the stool examinations were undertaken on the residents in Kilgeong-Ri. The infestation rate of crayfish with Paragonimus metacercaria was also examined in Konneung and Jeongha village in Kilgeong-Ri. The following results were obtained in this survey: As a whole, 16.6 per cent of the examiners showed positive reaction with sexual difference of 16.1% in male and 14.8 per cent in female. In age groups, the highest positive rate showed in middle school age group (17.1 per cent) than primary school (14.0 per cent) and high school age group (11.4 per cent). In regional differences of positive rates, the highest rate (28.4 per cent) showed at Yangdo Myun followed by Kilsang Myun (27.0 per cent), Hwado Myun (25.0 per cent), and Naega Myun(20.8 per cent). In the result of 208 residents Kilgeong-Ri, the positive of 36.1 per cent in male and 20.2 per cent in female. The highest positive rate showed in 10 to 19 age group (41.5 per cent). Among the residents in Kilgeong-Ri, Paragonimus eggs were detected in 11 residents by the sputum and the stool examinations. The infestation rate of crayfsh with Paragonimus metacercariae was 100 per cent (41 out of 41 crayfishes) in Konneung village, and 84.6 per cent (9 out of 13 crayfishes) in Jeongha village, Kilgeong-Ri The fluctuation of the infection rate of crayfish with Paragonimus metacercariae was decreased in Konneung village in 2 years after the treatment with Bilevon against 10 paragonimiasis patients.
parasitology-helminth-trematoda ; epidemiology-paragonimiasis ; Paragonimus westermani ; crayfish-Cambaroides similis