3.Technical improvement in rapid sectioning of paraffin-embedded tissue blocks.
Xiao-ping ZHENG ; Wen-xing XU ; Li-kang LUO ; Yan-fei XU
Chinese Journal of Pathology 2009;38(1):57-58
Humans
;
Leiomyoma
;
pathology
;
Microtomy
;
methods
;
Microwaves
;
Paraffin Embedding
;
methods
;
Specimen Handling
;
methods
;
Thyroid Neoplasms
;
pathology
4.Proteomic study of formalin-fixed and paraffin-embeded tissues.
Chinese Journal of Pathology 2009;38(10):718-720
7.Updates on antigen retrieval techniques for immunohistochemistry.
Shan-rong SHI ; Yan SHI ; Clive R TAYLOR
Chinese Journal of Pathology 2007;36(1):7-10
Antigens
;
analysis
;
genetics
;
Fixatives
;
Formaldehyde
;
Hot Temperature
;
Humans
;
Immunohistochemistry
;
methods
;
In Situ Hybridization
;
methods
;
Paraffin Embedding
;
Proteins
;
analysis
;
genetics
;
Staining and Labeling
;
methods
;
Tissue Fixation
;
methods
8.Experience on design of equipment for production of cell block.
Hong-min HE ; Li ZHANG ; Ya ZHANG ; Yu-bo REN
Chinese Journal of Pathology 2013;42(12):833-834
9.Antigen retrieval for immunohistochemistry performed on destained paraffin sections.
Xin-lan LUO ; Jie XU ; Yan-hui LIU ; Heng-guo ZHUANG
Chinese Journal of Pathology 2007;36(1):59-60
Antigens
;
analysis
;
immunology
;
Antigens, CD
;
analysis
;
immunology
;
Formaldehyde
;
Hot Temperature
;
Humans
;
Immunohistochemistry
;
methods
;
Neoplasms
;
metabolism
;
pathology
;
Paraffin Embedding
;
methods
;
Staining and Labeling
;
Tissue Fixation
;
methods
10.DNA extraction from formalin fixed and paraffin embedded tissues.
Journal of Forensic Medicine 2006;22(6):455-458
Owing to the DNA degradation induced by formalin and the obstruction of paraffin to DNA extraction, it is difficult to recover high-quality DNA from Formalin fixed and paraffin embedded tissue (FFPET). In recent years, a lot of researches indicate that the DNA extraction from FFPET can be developed by improving the pretreatment, optimizing the digestion condition of proteinase, simplizing the procedures of the DNA extraction, purifying the extracted DNA and so on, which may pave a way for popularizing FFPET in DNA analysis.
DNA/isolation & purification*
;
Forensic Medicine/methods*
;
Formaldehyde/chemistry*
;
Humans
;
Hydrogen-Ion Concentration
;
Paraffin Embedding/methods*
;
Polymerase Chain Reaction
;
Specimen Handling/methods*
;
Time Factors
;
Tissue Fixation
Result Analysis
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