OBJECTIVETo identify and assess multiple human papillomavirus types in condyloma acuminatum lesions from patients with genital warts in Beijing area, and compare different features between otherwise healthy and immunosuppressed patients.

METHODSPCR, RFLP and nucleotide sequencing analysis were used to determine HPV types from individual lesions.

RESULTSThe predominant type from other healthy patients was HPV6, secondly HPV11. The mean age of patients infected by HPV6 was lower than that of HPV11 and HPV6 + 11. While lesions from immunosuppressed patients were often contained HPV11 or mixed with HPV6. Besides, HPV types 16 and 53 were detected from infected lesions than other HPV types.

CONCLUSIONSHPV6 was the major pathogen of condyloma acuminatum, but infected patients were at lower ages. While HPV11 was most often detected from immunosuppressed patients. As a low risk virus in normal genital tract, HPV53 also could be a pathogen in genital warts.

Adult ; Condylomata Acuminata ; virology ; Female ; Humans ; Male ; Papillomaviridae ; classification ; isolation & purification ; Papillomavirus Infections ; Tumor Virus Infections ; Warts ; virology

Anus Neoplasms ; diagnosis ; etiology ; surgery ; Buschke-Lowenstein Tumor ; Carcinoma, Verrucous ; diagnosis ; etiology ; surgery ; Condylomata Acuminata ; etiology ; pathology ; surgery ; Human papillomavirus 16 ; Human papillomavirus 18 ; Humans ; Male ; Middle Aged ; Papillomavirus Infections ; complications ; Penile Neoplasms ; etiology ; pathology ; surgery ; Time Factors

Epidermodysplasia verruciformis is a very rare, chronic disease that's characterized by persistent human papillomavirus infection. This type of lesion may transform into malignant skin tumors, usually after the age of 30 years. We report here on a 33-year-old woman who presented with squamous cell carcinoma and actinic keratosis that developed from epidermodysplasia verruciformis. Her malignant skin lesions were effectively treated by surgical excision, cryotherapy and topical application of imiquimod.
Actins ; Adult ; Aminoquinolines ; Carcinoma, Squamous Cell ; Chronic Disease ; Cryotherapy ; Epidermodysplasia Verruciformis ; Female ; Humans ; Keratosis, Actinic ; Papillomavirus Infections ; Skin ; Skin Neoplasms

Human papillomavirus (HPV) is associated with cervical cell changes, genital warts, recurrent respiratory papillomatosis, laryngeal papillomatosis, head and neck cancer, and cervical cancer. Two commercial HPV vaccines have successfully been made available in the clinical field. This review covers the progress of cervical disease by understanding the nature of HPV infection, as well as the relationship between the host factors and HPV vaccine effectiveness. Among these host factors, microbiota has been revealed to influence the development and function of both the innate and adaptive immune systems. Therefore, the composition of the microbiome may ultimately affect vaccine efficacy. Understating the relationship between host factors and HPV infection/vaccine efficacy may prove to be useful in earlier diagnosis, as well as disease prophylaxis.
Condylomata Acuminata ; Diagnosis ; Head and Neck Neoplasms ; Humans* ; Immune System ; Microbiota ; Papilloma ; Papillomavirus Infections* ; Papillomavirus Vaccines ; Probiotics ; Uterine Cervical Neoplasms

Epidermodysplasia verruciformis (EV), a rare inherited disease, is believed to be associated with human papillomavirus (HPV) infection. EVER1/2 genes, dendritic cells, T lymphocytes, and the biological characteristics of HPV itself may play roles in the pathogenesis of HPV infection.
Dendritic Cells ; immunology ; Epidermodysplasia Verruciformis ; genetics ; immunology ; virology ; Humans ; Membrane Proteins ; genetics ; Mutation ; Papillomaviridae ; isolation & purification ; pathogenicity ; Papillomavirus Infections ; complications ; T-Lymphocytes ; immunology

PURPOSE: To evaluate the prevalence and type distribution of high-risk human papillomavirus (HPV) infection in genital warts of Korean men, and for the first time, to describe the risk factors associated with high-risk HPV infection in male genital warts. MATERIALS AND METHODS: In a single private clinic, 150 consecutive male patients with histopathologic-confirmed genital warts who underwent HPV genotyping by use of polymerase chain reaction (PCR) were included in this study. We detected HPV DNA in male genital warts and evaluated HPV type distribution, especially high-risk HPV types, by use of PCR. The associations between HPV prevalence and various characteristics, such as age, circumcision status, type of genital warts diagnosis (new vs. recurrent), number of lesions, site of lesions, and gross morphology, were assessed by use of unconditional multiple logistic regression. RESULTS: High-risk HPV types were detected in 31 cases (23.5%), and of these, 27 cases (20.5%) contained both high-risk and low-risk HPV types. The most frequently detected high-risk HPV types were HPV16 (6.8%), HPV33 (4.5%), HPV18 (2.3%), and HPV68 (2.3%). In particular, the prevalence of infection with HPV16 and/or HPV18 was 8.3% (11 of 132). In the multivariate analysis, lesions located at sites including the base of the penis or the pubic area, papular or mixed genital warts, and lack of circumcision significantly increased the association with high-risk HPV infection in male genital warts. CONCLUSIONS: The prevalence of high-risk HPV infection was substantial in male genital warts. The site and morphology of lesions and circumcision status were significantly associated with the prevalence of high-risk HPV infection.
Circumcision, Male ; Condylomata Acuminata* ; Diagnosis ; DNA ; Female ; Human papillomavirus 16 ; Humans* ; Logistic Models ; Male* ; Multivariate Analysis ; Papillomavirus Infections* ; Penis ; Polymerase Chain Reaction ; Prevalence* ; Risk Factors

OBJECTIVE: To assess the level and accuracy of understanding of human papillomavirus (HPV) infection among female public health personnel in Chonbuk province. METHODS: Nine hundred ninety-one female public health personnel were asked to complete a questionnaire assessing HPV awareness and specific knowledge about the virus. The questionnaire contained 11 demographic informations and nine true-false questions on knowledge about HPV infection. RESULTS: Questionnaires were completed by 546 women, of whom 145 (37.6%) had heard of HPV. Married women (P=0.019), those with a history of candida, genital warts (P<0.001), or an abnormal smear result (P=0.001), annually visitor for a Pap smear (P=0.023) were more likely to have heard of human papilloma virus. Medical doctor (38.6%) was the most common source of hearing of HPV. Overall percentage of knowledge among those who had heard of HPV was 56.3%. Responses indicated than more than 70% had up-to-date knowledge about several issues: HPV is the main cause of cervical cancer, HPV is sexually transmitted, The pill protects against HPV, and Men can carry HPV. Fewer than 50% knowledge of HPV infection were as follows: HPV viruses are divided to low-risk and high-risk type, HPV infections persist forever, condoms protect against HPV. CONCLUSION: In this well educated samples, awareness of HPV infection was poor. And also, knowledge about HPV infection was relatively low. We recommend that more educational effort is needed for improving women's knowledge and awareness of HPV infection.
Candida ; Condoms ; Condylomata Acuminata ; Female ; Hearing ; Humans ; Male ; Papilloma ; Papillomavirus Infections ; Public Health ; Surveys and Questionnaire ; Uterine Cervical Neoplasms ; Viruses

OBJECTIVETo explore the genotypes of human papilloma virus (HPV) in male patients with condyloma acuminate in Lishui area of Zhejiang Province, China.

METHODSUsing HybriMax, we identified the genotypes of HPV of the verruca samples from 110 male condyloma acuminate patients aged 16 - 65 years in Lishui area.

RESULTSHPV infection was detected in 107 (97.27%) of the condyloma acuminate patients, including 76 cases (71.02%) of simple infection and 31 cases (28.97%) of multiple infection. The peak age of infection was 21 - 40 years old. Totally, 14 genotypes of HPV were identified, including the high-risk genotypes as HPV16, 53, 52, 33, CP8304, 58, 51, 45, 66, 68, and 59, which accounted for 44.85% of the cases, and the low-risk ones as HPV6, 11, and 19 (43), with an infection rate of 93.45%.

CONCLUSIONThe low-risk genotype is a dominant type of HPV infection and the rates of high-risk genotype and multiple infection are high among the male condyloma acuminate patients in Lishui area.

Adolescent ; Adult ; Aged ; China ; Condylomata Acuminata ; virology ; Genotype ; Humans ; Male ; Middle Aged ; Papillomaviridae ; genetics ; Papillomavirus Infections ; virology ; Risk ; Young Adult

Objective: To investigate the distribution of the gene subtypes of human papillomavirus (HPV) in male patients with condyloma acuminatum (CA) and analyze the characteristics of the gene subtypes. METHODS: We extracted genomic DNA of the HPV virus from the genital tissue of 70 male CA patients, detected the DNA subtypes of HPV using the PCR-reverse dot hybridization technique, and analyzed the rates of different subtypes identified and their characteristics of distribution in different age groups. RESULTS: The male HPV-positive patients were mainly infected at the age of 20－39 years, primarily with high- and low-risk mixed infection of various subtypes, which accounted for 61.54% in the 20- to 29-year-olds and 42.86% in the 30- to 39-year-olds. Among the 70 CA patients, 22 HPV subtypes were identified, the top five subtypes including HPV 11 (21.08%), HPV 6 (19.46%), HPV 42 (6.49%), HPV 59 (6.49%) and HPV 53 (5.95%); 20 infected with a single subtype (28.57%), 19 with two subtypes (27.14%) and 31 with three or more (44.29%); and 30 infected with a low-risk single subtype (42.86%) and 40 with both high- and low-risk multiple subtypes (57.14%). CONCLUSIONS Male patients with CA are mainly infected with HPV 11 and HPV 6, with a significantly higher rate of multi-subtype than single-subtype infection, and the multi-subtype patients chiefly with high- and low-risk mixed infection. Men aged 20－39 years old are most commonly affected by CA.
Adult ; Condylomata Acuminata/virology* ; DNA, Viral/genetics* ; Genotype ; Humans ; Male ; Papillomaviridae/genetics* ; Papillomavirus Infections/virology* ; Young Adult

OBJECTIVETo determine the different rates of human papillomavirus types 6 (HPV-6) and 11 (HPV-11) infection in biopsy samples from pointed condyloma patients, and to construct prokaryotic expression system of the major capsid protein L1 of the virus so as to establish an ELISA for detecting the expression of L1 gene in the biopsy samples.

METHODSUsing a double PCR based on the L1 gene of HPV-6 and HPV-11, the infection rates of HPV-6 and HPV-11 in the biopsy samples were determined. The whole length of HPV-6 L1 gene was amplified using PCR and the target amplification fragment was sequenced after T-A cloning. The prokaryotic expression system pET32a-L1-E. coli BL21 (DE3) was constructed and SDS-PAGE was used to measure the expression of the target recombinant protein rL1. Rabbit anti-rL1 serum was prepared and immuno-diffusion assay was applied to examine the antiserum titer. ELISA was established to detect the expression of L1 gene in the biopsy samples.

RESULTSIn the biopsy samples from 116 pointed condyloma patients, 92.2% (107/116) were detectable for HPV-6 and/or HPV-11. Of the 107 positive samples, 70.1% (75/107) and 23.4% (25/107) were positive for HPV-6 or HPV-11 alone and 6.5% (7/107) were coinfected with both HPV-6 and HPV-11 respectively. When compared with the reported corresponding sequences, the homology of nucleotide and sequence of the cloned HPV-6 L1 gene was from 99.20% - 99.93% while its putative amino acid sequence homology was from 99.80% - 100%, suggesting IPTG could induce the expression of rL1. The immuno-diffusion titer of the rabbit anti-rL1 serum was 1:4. 88.8% (103/116) of the biopsy samples were the major capsid protein L1 detectable.

CONCLUSIONA prokaryotic expression system of HPV-6 L1 gene, a double PCR assay for HPV-6 and HPV-11 genotyping, and an ELISA assay for detecting the major capsid protein L1 were successfully established in this study. The pointed condyloma patients in Zhejiang area mainly infected with HPV-6. The HPV in the focus frequently expressed major capsid protein L1.

Animals ; Biopsy ; Capsid Proteins ; genetics ; Condylomata Acuminata ; pathology ; virology ; Enzyme-Linked Immunosorbent Assay ; Gene Expression ; Human papillomavirus 11 ; genetics ; isolation & purification ; Human papillomavirus 6 ; genetics ; isolation & purification ; Humans ; Papillomavirus Infections ; virology ; Polymerase Chain Reaction ; Rabbits ; Sequence Homology, Nucleic Acid