OBJECTIVE: To observe pathological changes and apoptosis in rats myocardial cells after Macleaya cordata total alkaloids poisoning, and to provide some references for Macleaya cordata total alkaloids poisoning detection. METHODS: An experimental model of Macleaya cordata total alkaloids poisoning was established, and the technology of TUNEL staining was used.The results were analyzed by computer image analysis competitive system. RESULTS: Quantities of apoptosis in myocardial cells in poisoning groups were much more than those in the control groups at different tages (P<0.01). In addition the quantities of apoptosis were different after different poisoning duration. CONCLUSION Although clinical symptoms was not obvious and could not be detected by poison analysis. Pathological changes induced by Macleaya cordata total alkaloids could be found through the apoptosis detection.
Acute Disease ; Animals ; Apoptosis/drug effects* ; Cell Count ; Disease Models, Animal ; Female ; Immunohistochemistry ; In Situ Nick-End Labeling ; Male ; Myocardium/pathology* ; Myocytes, Cardiac/drug effects* ; Papaveraceae/chemistry* ; Papaverine/poisoning* ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Staining and Labeling ; Time Factors

OBJECTIVE: To observe the expression of Bcl-2 and Bax proteins in rat's myocardial cells after Macleaya cordata alkaloids poisoning, and to provide certain molecular biology references for the detection of Macleaya cordata alkaloids poisoning. METHODS: Experimental model of Macleaya cordata alkaloids poisoning was established, the expression levels of Bcl-2 and Bax proteins in these cells were detected by immunohistochemistry, and the results were analyzed by computer image system. RESULTS: The expression levels of Bcl-2 and Bax proteins in myocardial cells in poisoning groups were much greater than those in the control groups (P<0.05). CONCLUSION If the clinical symptoms may not be obvious, the detection of Bcl-2 and Bax proteins level by immunohistochemistry still could be ancillary method.
Animals ; Disease Models, Animal ; Female ; Immunohistochemistry ; Male ; Myocardium/pathology* ; Myocytes, Cardiac/pathology* ; Papaveraceae/chemistry* ; Papaverine/poisoning* ; Proto-Oncogene Proteins c-bcl-2/metabolism* ; Rats ; Rats, Sprague-Dawley ; Staining and Labeling ; Time Factors ; bcl-2-Associated X Protein/metabolism*