1.Effects of Burdock Leaf Extract NBY-4 on Oxidative Damage of Vascular Endothelial Cells
Kaixia WANG ; Jingjing YAN ; Panxia CAO ; Menghui HU ; Liping DAI ; Hong WU
World Science and Technology-Modernization of Traditional Chinese Medicine 2023;25(12):3899-3907
Objective To investigate the function of burdock leaf extract NBY-4 in protecting vascular endothelial cells from oxidative damage.Methods The oxidative damage model of vascular endothelial cells was established with hydrogen peroxide(H2O2).ROS,LDH,SOD,apoptosis rate and the expression of apoptosis were measured;Further test the nuclear transcription factor-κB(NF-κB).Results In the model group,H2O2 increased the apoptosis rate of endothelial cells,increased the ratio of Bax/Bcl-2 and the expression of Caspase-3,promoted the production of ROS and LDH in endothelial cells,and inhibited the level of SOD,resulting in oxidative damage to endothelial cells(P<0.05).NBY-4 could slow down the process of these effects;At the same time,NBY-4 can reduce NF-κB in endothelial cells.Conclusion NBY-4 can inhibit endothelial cell apoptosis and antioxidant damage by NF-κB signaling pathway.
2.Isorhapontigenin protects against doxorubicin-induced cardiotoxicity
Panxia WANG ; Minghui WANG ; Yuehuai HU ; Jianxing CHEN ; Yanjun CAO ; Cui LIU ; Zhongkai WU ; Juan SHEN ; Jing LU ; Peiqing LIU
Acta Pharmaceutica Sinica B 2021;11(3):680-693
As an effective anticancer drug, the clinical limitation of doxorubicin (Dox) is the time- and dose-dependent cardiotoxicity. Yes-associated protein 1 (YAP1) interacts with transcription factor TEA domain 1 (TEAD1) and plays an important role in cell proliferation and survival. However, the role of YAP1 in Dox-induced cardiomyopathy has not been reported. In this study, the expression of YAP1 was reduced in clinical human failing hearts with dilated cardiomyopathy and Dox-induced