Acute pancreatitis( AP)is a common gastrointestinal emergency,which comprises mild acute pancreatitis ( MAP),moderately severe acute pancreatitis( MSAP)and severe acute pancreatitis( SAP). Most patients with MAP and MSAP present as a mild,self-limiting disease with low morbidity and mortality,however,patients who suffer from SAP will experience serious local and/or systemic complications associated with high mortality. Therefore,early assessment of disease severity is important for clinical management. Procalcitonin( PCT),a pro-peptide of calcitonin,is an inflammatory mediator that can be used to differentiate bacterial and non-bacterial infection. Recently,the effect of PCT on AP has attracted more and more attentions. This article reviewed the advances in studies on PCT and AP.

Objective To establish and optimize AFLP reaction system used in providing necessary technique basis for genetic diversity analysis in Asarum sieboldii.Methods Leaves of A.sieboldii were used as experimental materials to analyze various essential elements of the whole process,such as quality of extracted DNA,time of restriction digest and ligation,concentration of Mg2+,primers and Taq DNA polymerase during PCR amplification etc.,so that the most optimal AFLP reaction system could be built up. Results The optimal AFLP reaction system of A.sieboldii has been constructed: in the genomic DNA extraction,mercaptoethanol being utilized and samples being incubated about 30 min at 65 ℃;the genomic DNA being digested by Trul Ⅰ and Pst Ⅰ for 3 h respectively;in PCR amplification,the final concentration of Mg2+ being 1.5 mmol/L and the volume of Taq DNA polymerase being 0.2 ?L.Conclusion The present reaction system for A.sieboldii is able to gain the favorable results of AFLP analysis and can be used in the genetic diversity research of the species.

Objective To explore the hearing screening, and the change and outcome of hearing impairment of high risk infants. Meth-ods From March, 2015 to March, 2016, 336 high risk infants were screened with otoacoustic emissions (OAE), auditory brainstem response (ABR) and brainstem auditory evoked-potential (BAEP) 0-1, 3, 6, 12 months after born, respectively. Results Among the 336 high risk in-fants, 29 failed the examinations within the 1st month, 37 cases failed in the 3rd month, 27 cases recovered in the 9th month, and 7 cases re-covered in the 12th month, 3 cases were finally diagnosed as deafness (0.89%). Conclusion OAE, ABR combining with BAEP examination may obtain comprehensive diagnosis of hearing impairment for high risk infants, continuous listening comprehension monitoring can effec-tively dynamically observe the hearing impairment, changes and outcome of high risk infants.

Objective To observe the clinical effect of Zhengan Xifeng decoction in the treatment of Parkinson's disease.Methods From January 2013 to January 2017,100 patients with Parkinson's disease in the People's Hospital of Dongping County were included in the study and they were randomly divided into two groups according to the digital table,with 50 cases in each group.The control group was treated with Madopar,the observation group was treated with Madopar combined with Zhengan Xifeng decoction.Mter 12 weeks of treatment,the clinical efficacy,incidence of adverse reactions,unified Parkinson's disease score,ADL score,cognitive function index of the two groups were compared.Results The total effective rate of the observation group was 92％ (46/50),which was significantly higher than 74％ (37/50) of the control group (x2 =5.741,P ＜ 0.05).The incidence rate of adverse reactions of the observation group was 6％ (3/50),which of the control group was 4％ (2/50),the difference was not statistically significant(x2 =0.211,P ＞ 0.05).After treatment,the unified Parkinson's disease score,ADL score,Montreal cognitive function score,mini mental state score in the observation group were (49.93 ± I0.86) points,(85.71 ± 14.53) points,(25.93 ± 2.76) points,(23.81 ± 4.12) points,respectively,which in the control group were (60.56 ± 11.49) points,(71.46 ± 14.02) points,(22.56 ± 2.65) points,(19.42 ±.4.05) points,respectively,there were statistically significant differences between the two groups (t =4.754,4.990,6.228,5.373.all P ＜ 0.05).Conclusion Zhengan Xifeng decoction in the treatment of Parkinson's disease can effectively improve the patients' cognitive function,daily life ability,alleviate the symptoms of Parkinson's disease.

Objective: To observe the clinical effect of Xiayuxue decoction in the treatment of endometriosis. Methods: 88 patients with endometriosis were selected and randomly divided into two groups by drawing lots, with 44 cases in each group.The control group received danazol treatment, the observation group was given TCM Xiayuxue decoction.Before and after treatment, the serum endocrine hormones[serum estradiol (E₂), testosterone(T), progesterone(P), luteinizing hormone(LH), follicle stimulating hormone(FSH)]contents and humoral immunity(C₃, C₄) levels, and the clinical effect were compared between the two groups. Results: After treatment, the E₂, T, P, LH, FSH levels of observation group were (109.47±20.67)ng/L, (441.23±120.14)ng/L, (15.11±4.13)g/L, (9.85±2.34)IU/L, (3.05±0.90)IU/, respectively, which of the control group were (100.22±17.45)ng/L, (500.47±110.24)ng/L, (15.34±4.20)g/L, (9.01±1.04)IU/L, (3.08±0.91)IU/, respectively, there were statistically significant differences between the two groups(t=2.27, 2.41, 0.26, 2.18, 0.16, all P<0.05). The C₃, C₄ levels of the observation group were (1.09±0.25)g/L, (0.52±0.10)g/L, respectively, which of the control group were (1.00±0.10)g/L, (0.77±0.46)g/L, respectively, there were statistically significant differences between the two groups(t=2.23, 3.52, all P<0.05). The total effective rate of the observation group was 95.45%, which was significantly higher than 79.55% of the control group (χ²=5.09, P<0.05). Conclusion Xiayuxue decoction in the treatment of endometriosis can improve the endocrine hormone and humoral immunity of patients, and the effect is excellent.

Objective:To investigate the role and regulatory mechanism of miR-125b-1-3p in rotavirus replication.Methods:MA104 cells were infected with rotavirus after upregulation and down-regulation of miR-125b-1-3p, respectively. The expression of miR-125b-1-3p and the copy number of rotavirus were analyzed by RT-PCR. The effect of miR-125b-1-3p on the protein expression of rotavirus was analyzed by immunofluorescence. The expression of related proteins involved in the regulation of miR-125b-1-3p was analyzed by Western blot analysis.Results:After rotavirus infection, the expression level of miR-125b-1-3p was significantly up-regulated, the copy number of VP7 and NSP3 gene of rotavirus decreased after up-regulation of miR-125b-1-3p, and the copy number of VP7 and NSP3 gene of rotavirus was significantly increased after down-regulation of miR-125b-1-3p.The fluorescence number of rotavirus protein decreased after upregulation of miR-125b-1-3p expression level, and increased after down-regulation of miR-125b-1-3p expression level. The activity of PI3K/Akt pathway was inhibited 16 h after rotavirus infection, and the up-regulation of miR-125b-1-3p could inhibit the activation of PI3K/Akt pathway.Conclusions:MiR-125b-1-3p inhibits rotavirus replication by regulating the PI3K/Akt pathway. These results provide an experimental basis for exploring the specific regulatory mechanism between miR-125b-1-3p and PI3K/Akt pathway, and provide a target for anti-infection therapy of rotavirus.

OBJECTIVETo investigate the effect of occupational exposure to toluene diisocyanate (TDI) on the workers' health.

METHODSA total of 76 workers exposed to TDI (exposure group) and 64 management staff members (control group) were selected from a factory as the study subjects. Area sampling was performed for the place with exposure to TDI according to the method in GBZ 159-2004 Specifications of air sampling for hazardous substances monitoring in the workplace, and gas chromatography was applied to measure the concentration of TDI in workplace air. The workers' personal information was collected with questionnaire, pulmonary ventilation function was determined with a portable spirometer, hematological parameters were analyzed by automatic blood analyzer and blood chemistry analyzer, and the indicators of oxidative damage and energy metabolism were measured by the reagent kit provided by Nanjing Jiancheng Bioengineering Institute. SPSS 17 software was applied for statistical analysis.

RESULTSThe exposure group had significantly lower forced vital capacity (FVC), forced expiratory volume in 1 second(FEV1.0), and FEV1.0/FVC ratio than the control group (P <0.05). Compared with the control group, the exposure group had significantly higher red blood cell count, platelet distribution width, mean platelet volume, lymphocyte count, and neutrophil count(P<0.01), and significantly lower activities of lactate dehydrogenase(LDH), superoxide dismutase, and succinodehydrogenase (SDH)(P <0.01). In the exposure group, the length of exposure was negatively correlated with the activities of SDH and LDH in the serum (r=-0.319, P <0.05; r=-0.239, P <0.05), and the length of exposure was not found to be correlated with the activity of SOD and pulmonary function indices.

CONCLUSIONTDI can induce inflammatory response and lung ventilation function impairment in workers exposed to TDI, as well as oxidative stress and imbalance of energy metabolism. Therefore, it can cause damage to workers' health, and protective measures should be enhanced.

Case-Control Studies ; Erythrocyte Count ; Forced Expiratory Volume ; Humans ; Inflammation ; physiopathology ; L-Lactate Dehydrogenase ; blood ; metabolism ; Leukocyte Count ; Lung ; physiopathology ; Occupational Exposure ; adverse effects ; Pulmonary Ventilation ; Succinate Dehydrogenase ; blood ; metabolism ; Superoxide Dismutase ; metabolism ; Toluene 2,4-Diisocyanate ; adverse effects ; Vital Capacity

ObjectiveTo explore psychological and behavioral characteristics of children with autism spectrum disorder (ASD) using Psycho-educational Profile (Third Edition) (PEP3). MethodsFrom October, 2021 to October, 2022, 192 children with ASD without intervention in the Binzhou Medical University Hospital were selected as observation group, and 96 healthy children who visited at the same time were selected as control group. They were assessed with PEP3. ResultsThe development of receptive language significantly delayed behind expressive language (t = 5.383, P < 0.001) in the observation group. The cognitive, expressive language, receptive language, fine motor, gross motor, imitation and personal self-care correlated with age (r = 0.540 to 0.795, P < 0.001). The Cronbach's α of PEP3 for the observation group was 0.810 to 0.947. The original score of each subtest of PEP3 was less in the observation group than in the control group (|t| > 4.267, P < 0.001). ConclusionThe development of receptive language retards behind the expressive language in children with ASD. Cognitive and motor functions develop with age, while the correlation between maladaptive behavior and age is weak. PEP3 is reliable in internal consistency, and valid in discrimination.

ObjectiveTo explore the typical behavior characteristics of children with autism in symbolic play and the value of Symbolic Play Test (SPT) in the early identification of autism. MethodsFrom November, 2021 to September, 2022, a total of 260 children with language problems were collected from Department of Children's Health Care of Binzhou Medical University Hospital. A total of 193 children with autism were as observation group and 67 normal children were as control group. All children played symbolic games. The typical behavioral characteristics of children with autism in SPT were explored, and a reliability and validity analysis based on the results of SPT was conducted. They were assessed with the adaptive and personal social scores of Gesell Development Scale, and the correlation of the scores of Gesell Development Scale and the score of SPT was analyzed. ResultsThe Cronbach's α coefficient of SPT of children with autism was 0.835 to 0.935, and the total score of SPT, the scores of surrogate object, fictional attribute and fictional object were positively correlated with each other (r > 0.607, P < 0.001). The SPT scores decreased in the observation group (t > 9.615, P < 0.001), and SPT score positively correlated with adaptability and personal-social development quotient (r > 0.609, P < 0.001). ConclusionTypical behavior of children with autism can be reflected in symbolic play, and SPT can provide clues for early identification of autism.