1.Progress of research on oncogene PTEN in thyroid malignant tumors
Fan LIU ; Panlong WANG ; Yalan ZHANG ; Yahui CHAI ; Ailin SONG
Chinese Journal of Endocrine Surgery 2023;17(3):364-366
The phosphatase and tensin homolog deleted on chromosome ten (PTEN) is a common oncogene located in the 10q23.3 region on the long arm of human chromosome l0, which regulates a variety of biological processes such as proliferation, survival, cell structure, motility, energy metabolism and genomic stability. Inactivation of PTEN is prevalent in almost all malignancies and correlates with tumor progression. Thyroid malignancies are among the most common endocrine malignancies, and PTEN has been shown to be critically associated with their development. The aim of this review is to describe the structural function of PTEN, as well as to summarize and discuss the recent findings of PTEN in thyroid malignancies.
2.Study of Recellularized Human Acellular Arterial Matrix Repairs Porcine Biliary Segmental Defects
Wei LIU ; Sheng Ning ZHANG ; Zong Qiang HU ; Shi Ming FENG ; Zhen Hui LI ; Shu Feng XIAO ; Hong Shu WANG ; Li LI
Tissue Engineering and Regenerative Medicine 2019;16(6):653-665
BACKGROUND: With the popularity of laparoscopic cholecystectomy, common bile duct injury has been reported more frequently. There is no perfect method for repairing porcine biliary segmental defects.METHODS: After the decellularization of human arterial blood vessels, the cells were cultured with GFP⁺ (carry green fluorescent protein) porcine bile duct epithelial cells. The growth and proliferation of porcine bile duct epithelial cells on the human acellular arterial matrix (HAAM) were observed by hematoxylin-eosin (HE) staining, electron microscopy, and immunofluorescence. Then, the recellularized human acellular arterial matrix (RHAAM) was used to repair biliary segmental defects in the pig. The feasibility of it was detected by magnetic resonance cholangiopancreatography, liver function and blood routine changes, HE staining, immunofluorescence, real-time quantitative PCR (RT-qPCR), and western blot.RESULTS: After 4 weeks (w) of co-culture of HAAM and GFP? porcine bile duct epithelial cells, GFP⁺ porcine bile duct epithelial cells grew stably, proliferated, and fused on HAAM. Bile was successfully drained into the duodenum without bile leakage or biliary obstruction. Immunofluorescence detection showed that GFP-positive bile duct cells could still be detected after GFP-containing bile duct cells were implanted into the acellular arterial matrix for 8 w. The implanted bile duct cells can successfully resist bile invasion and protect the acellular arterial matrix until the newborn bile duct is formed.CONCLUSION: The RHAAM can be used to repair biliary segmental defects in pigs, which provides a new idea for the clinical treatment of common bile duct injury.
Bile
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Bile Ducts
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Blood Vessels
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Blotting, Western
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Cholangiopancreatography, Magnetic Resonance
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Cholecystectomy, Laparoscopic
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Coculture Techniques
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Common Bile Duct
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Duodenum
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Epithelial Cells
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Fluorescent Antibody Technique
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Humans
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Infant, Newborn
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Liver
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Methods
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Microscopy, Electron
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Polymerase Chain Reaction
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Swine
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Tissue Engineering
3.Study on Compatibility Rationality of Panlongqi Tablets in Treatment of Osteoarthritis Based on Network Pharmacology
Lu WANG ; Ying XU ; Chun-fang LIU ; Teng-fei HAN ; Rui-qin CUI ; Na LIN
Chinese Journal of Experimental Traditional Medical Formulae 2020;26(18):166-174
Objective:To explore the compatibility of Panlongqi tablets in the treatment of osteoarthritis. Method:Network pharmacology was used to predict and screen the targets and pathways related to osteoarthritis of 59 compounds in Panlongqi tablets including activating blood circulation and removing stasis group(ACRG),expelling wind-damp group(EWDG)and tonifying liver and kidney group(TLKG). Through data integration analysis, the characteristics and compatibility rules of this prescription in preventing and treating osteoarthritis were analyzed. Result:The 59 compounds can act on 70 osteoarthritis(OA) related targets, mainly involving inflammatory stimulation response, cell proliferation, cell metabolism, immune regulation and other related processes. Pathway enrichment analysis involved inflammatory response, cartilage degeneration, immune regulation, bone metabolism and other related pathways. Conclusion:The three drugs play different regulatory roles in the pathogenesis of OA, such as inflammation, chondrocyte apoptosis and metabolism, extracellular matrix degradation, and bone metabolism. Among them, promoting blood circulation and removing blood stasis were mainly related to anti-inflammatory and analgesia, the wind-dampening group was mainly involved in regulating immunity and inflammation, and the liver-kidney group was more related to bone metabolism and chondrocyte apoptosis.
4.Analgesic Effect of Panlongqi Tablet on Rats with Chronic Inflammatory Pain
Ke-xin JIA ; Chun-fang LIU ; Jin-xia WANG ; Yi-qun LI ; Teng-teng XU ; Rui-rui MING ; Teng-fei HAN ; Qi WANG ; Na LIN
Chinese Journal of Experimental Traditional Medical Formulae 2020;26(15):61-68
Objective:To observe the analgesic effect of Panlongqi tablet(PLQT) on rats with chronic inflammatory pain, and to explore mechanism of the action preliminarily from the perspective of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-