No abstract available.
Amylases/analysis ; Bicarbonates/analysis* ; Chronic Disease ; Endoscopy, Digestive System ; Human ; Pancreas/physiology* ; Pancreatic Juice/secretion ; Pancreatic Juice/enzymology ; Pancreatic Juice/chemistry* ; Pancreatitis/metabolism ; Pancreatitis/diagnosis*

AIMTo know the effect of cysteamine on the pancreatic secretion and enzyme activity in geese.

METHODSEight adult geese fitted chronic pancreatic and duodenal cannulas were used to evaluate the effect of cysteamine (CS) on the pancreatic secretion and enzyme activity. The experiment was consist of control and treated phase. CS was added in the diet at the dosage of 100 mg/kg bw on the first day of treated phase. The birds were free fed at daytime (8:00-20:00) and fasted at nighttime (20:00-8:00). The pancreatic juice samples were collected continuously for three days in each phase.

RESULTSCS increased the average rate of pancreatic secretion by 240.16% (P < 0.01), in which that of daytime was elevated by 234.45% (P < 0.01), while that of nighttime elevated by 253.70% (P < 0.01). The secretion volume at daytime was more than that of night. CS increased trypsin activity by 49.05% (P < 0.01), whereas lipase and amylase activity was reduced by 25.44% (P < 0.01) and 21.95% (P < 0.01) separately. The one hour total activity of trypsin, lipase and amylase were elevated by 406.88% (P < 0.01), 153.58% (P < 0.01) and 166.59% (P < 0.01) respectively. Ratios of pancreatic secretion were different between day and night.

CONCLUSIONThese results indicate that CS can affect the pancreatic juice secretion and pancreatic enzyme activity by depleting the somatostatin, so that benefits to improve the digestive foundation and supply more nutrition for quickly growing in geese.

Animals ; Cysteamine ; pharmacology ; Geese ; physiology ; Pancreas ; drug effects ; enzymology ; secretion ; Pancreatic Juice ; secretion ; Pancreatin ; metabolism

BACKGROUND/AIMS: There has been lack of data on the secretion of the pancreatic juice after pancreatoduodenectomy. Thus, surgeons have difficulties in predicting the clinical sequences of pancreaticojejunostomy leakage or other pancreatic surgery. METHODS: This study employed 15 patients who underwent pylorus-preserving pancreatoduodenectomy (PPPD) with total external drainage of the pancreatic duct from January 2000 to June 2001. RESULTS: These patients were divided into the normal pancreas group (n=11) and chronic pancreatitis- accompanied group (n=4). Excretion of pancreatic juice was suppressed in a few days after PPPD. Then, it gradually increased and became constant at the postoperative day 4. The amount of excreted pancreatic juice in the normal pancreas group was 229 +/- 99, 85~385 (mean, range) mL/day at the postoperative day 7 before the initiation of oral intake, and was increased to 314 +/- 222, 110~910 (mean, range) mL/day at the 7th day after the initiation of food intake. The usage of octreotide during early fasting period had no influence on the excretion of the pancreatic juice in the normal pancreas group. CONCLUSIONS: We observed the real amount of secreted pancreatic juice after PPPD. These data may be helpful to interpret and manage the postoperative conditions such as pancreatic leakage or pancreatic exocrine insufficiency.
Chronic Disease ; English Abstract ; Humans ; Pancreatic Juice/*secretion ; *Pancreaticoduodenectomy ; Pancreatitis/physiopathology

Twenty dyes which previously have been claimed to be excreted in pancreatic juice were reinvestigated to determine to what extent they could be eliminated through the pancreas. Exogenous secretin or cholecysto-kinin-pancreozymin(CCK-PZ) stimuli were used in dogs which had been given intravenous dye solutions at the rate of 1mg/min. In this experiment among the twenty dyes, only six were found to be eliminated through the pancreas. The intensity of dye color in pancreatic juice was estimated photometrically or macroscopically. The dye color intensity decreased as follows; basic fuchsin, acridine red, new fuchsin, rhodamin B, phenol red and rhodamin 6G. Basic fuchsin consistently appeared in CCK-PZ stimulated juice. However, it was seen in only a scant amount or not at all in juice stimulated by purified Vitrum (Sweden) secretin. Similar findings were observed in cats and conscious pigs. The content of basic fuchsin in pancreatic juice was more related to changes in the enzyme concentration than to other components. The chloride content of the juice was related to the amylase or basic fuchsin secretion. However, the chloride content was inversely related to the secreted volume. Vagal stimulation or the administration of parasympathomimetics produced a juice rich in enzyme content, but the dye response to vagal stimulation was weak. Usually the volume of secreted pancreatic juice following stimulation by Boots (England) secretin is greater than stimulated by purified Vitrum preparation. Basic fuchsin was slightly reduced during its elimination from pancreas or when present in alkaline pancreatic juice. Adding acid and formaldehyde revived the color. The acridine red and other pyronine dyes caused the juice to fluorescence. This effect lasted over 24 hours.
Animal ; Dogs ; Dyes/metabolism* ; Pancreas/metabolism* ; Pancreatic Juice/secretion* ; Rosaniline Dyes/metabolism*

OBJECTIVETo study the changes of canine Oddi sphincter (SO) function after pancreas transplantation with bladder drainage and the effect on the graft function.

METHODSNormal canine SO, transplant canine SO and canine SO in vitro manometry were performed by triple lumen catheter. At the same time, pancreas endocrine and exocrine function after transplantation were determined. After transplantation, anti-reflux function of graft SO was also measured.

RESULTSEndocrine and exocrine function of all the transplanted dogs showed that pancreas graft function was good. Basal pressure of SO in control group was (18.5 +/- 2.8) mm Hg (1 mm Hg = 0.133 kPa). The contraction frequency was (9.7 +/- 1.5) per min, the contraction amplitude was (47.1 +/- 5.5) mm Hg, the motility index was (236 +/- 56). After transplantation, basal pressure increased to (27.8 +/- 2.8) mm Hg, frequency increased to (13.1 +/- 1.9) per min, amplitude decreased significantly to (8.3 +/- 1.8) mm Hg. There was no significant difference of motility index. Basal pressure of SO in vitro increased significantly to (37.2 +/- 5.1) mm Hg. Phasic contraction was not absent. After transplantation, the pressure in the bile duct residual did not increase in accordance with the increase of bladder pressure.

CONCLUSIONSAfter pancreas transplantation with bladder drainage, Basal pressure and frequency of canine SO could increase while amplitude could decrease, which provide the anti-reflux function of graft SO and may serve as an obstacle to pancreatic juice flow.

Animals ; Dogs ; Drainage ; methods ; Female ; Male ; Pancreas Transplantation ; methods ; physiology ; Pancreatic Juice ; secretion ; Sphincter of Oddi ; physiology ; Transplantation, Homologous ; Urinary Bladder ; surgery