AIM:To investigate the effects of propofol on ammonia-induced neocortical astrocyte aquaporin-4 expression in rats and its mechanism. METHODS:Astrocytes were separated from newborn Sprague Dawley rats. Glial fibrillary acidic protein,the specific protein of astrocyte,was labeled by cell immunofluorescence method. The purity of astrocyte achieved to 95% was considered to be used in the study. Grouping:cultured astrocytes were randomly divided into 5 groups (n=3):normal control group (N); ammonia-incubated for 24 h group (NH_4Cl-24 h); p38 antagonist SB203580 (10 μmol/L) pretreated group (SB); propofol (10 μmol/L) pretreated group (P); solvent (DMSO) control group (C). SB203580,propofol and DMSO were pretreated for 30 min before astrocytes were exposed to NH_4Cl. Cell morphology was assessed by light microscopy. The expression of aquaporin-4; p38 and p-p38 were detected by Western blotting. RESULTS:Astrocytes were found significant swelling when exposed to 5 mmol/L NH_4Cl in NH_4Cl-24 h group compared to control group. Pretreatment with propofol and SB203580 decreased astrocyte swelling. No significant change in total p38 in all groups was observed (P>0.05) by Western blotting analysis,while the levels of p-p38 and AQP4 in group SB and group P were significantly decreased compared to group C and group NH_4Cl-24 h (P<0.05).CONCLUSION:The expression of AQP4 is regulated by p38 pathway. Propofol pretreatment down-regulates aquaporin-4 expression through preventing the ammonia-induced p38 phosphorylation.

Objective To study effect of traditional Chinese medicine chrysanthemum decoction on RSV infection after A549 cells chemotactic factor.Methods Subjects from The Second Hospital Affilated To Suzhou University in recent days, 64 cases of patients diagnosed with RSV effected pneumonia by clinical examination, RSV infection A549 cell model was established and the infected cells were cultured, chrysanthemums with TCM decoction and conventional antiviral observed its effect on RSV infection cell survival, compared two groups of cell SAP was determined by ELISA kit and the expression of RANTEs and chemokines MCP-1.Results Chrysanthemum, according to the results of in vitro cell culture studies traditional Chinese medicine decoction had the effect of directly inhibit the growth of virus replication, and there were some positive effects RSV infected A549 cells, MCP-1 and RANTEs expression or release significantly increased ( P<0.05 ) .Chrysanthemum Chinese medicine decoction and antiviral drugs after the intervention, the expression of MCP-1 and RANTES reduced significantly (P<0.05), but overall volume was still significantly higher than the normal. Conclusion Chrysanthemum Chinese medicine decoction can inhibit RSV infected A549 cells release chemokine RANTEs and MCP-1 to some extent, and then reduce the airway inflammation reaction of children.

Childhood tuberculosis remains a serious public health threat which draws more and more attention.The unspecific symptoms or signs and the paucibacillary nature of childhood tuberculosis make it hard to diagnose which delays appropriate treatment and increases disease burden and mortality.Molecular methods such as polymerase chain reaction (PCR),real-time PCR,and DNA line probe based on mycobacterium tuberculosis specific nucleotide sequence will make a quick and reliable diagnosis of childhood tuberculosis.

AIM:To establish the method for quality control of Suhong Tongluo Tincture(eugenol and methylsalicylate). METHODS: Gas phase chromatogram approach was used to determine the content of eugenol and(methylsalicylate.) The chromatogram condition consisted of 10% PEG-20M,carrier was N2(99.999%),temperature of the entrance of the column was at 230(?C),temperature of the detector was at 250(?C);column temperature was at 70(?C)(4 min),3(?C)/min→90(?C)(4 min),then 13(?C)/min→200(?C)(3 min);0.5 ?L Sample quantity. RESULTS: The linear range of methylsalicylate was 2.41-14.21 ?g,r=0.999 7,the average recovery was (99.76%),RSD=(2.64%(n=6).) The linear range of eugenol was 2.43-14.34 ?g,r=0.999 96,the average recovery was (99.33%),RSD=2.47%(n=6). CONCLUSION: This method is simple,quick,accurate and reliable for the quality control of Suhong Tongluo Tincture.

Objective To analysis clinical significance of joint detection of serum Cys C,Hcy and mAlb in HDCP patients. Methods Selected 92 cases of HDCP patients in Guangji hosipital of Hezhou from Jun 2011 to Jun 2014 as observation group.They were divided into three groups (43 patients of gestational hypertension as group A,32 patients with mild pre-eclampsia as group B,17 patients of severe preeclampsia as group C)and chose 50 cases of healthy maternal the same period in the hospital as control group.Enhanced turbidimetric method for the determination of serum Cys C,Hcy,immune scatter-ing method for urinary mAlb,the results were analyzed.Results Observation group with the level of serum Cys C,Hcy and urinary mAlb was rise with the damage degree aggravating,group C rise significantly (F =4.567,P <0.001).Observation group serum Cys C,Hcy and abnormal detection rate of urinary mAlb level was rise at the same time renal damage degree aggravating,group C rise significantly (χ2 = 12.842,P <0.001).Three joint detection indices abnormal detection rate was significantly higher than that of single detection (χ2 =5.869,P <0.001),the detection sensitivity of 95.70% has significant advantage (χ2 =15.992,P <0.001).Conclusion The joint detection of renal function of serum Cys C,Hcy and mAlb in HDCP patients was better than the single detection,which is helpful for the early detection of HDCP patients with renal damage,it’s worthy clinical promotion.

Objective To learn how well these items of Hubei Province meet the quality standards of allowed imprecision. Methods Collected the indoor quality control data of median concentration levels from the laboratories which participated the project of interlaboratory comparisons of clinical chemistry indoor quality control data in Hubei Province.This paper was to analyze the variation coefficient of indoor quality control for 21 routine clinical chemistry examination items which were K,Na,Cl,TCa,P,GLu,Urea,UA,Cr,TP,Alb,TC,TG,ALT,AST,TBil,ALP,AMS,CK,LDH andγ-GT.The other objec-tive was to learn how well these items of Hubei Province meet the quality standards of allowed imprecision.Then took the 1/3 TEa,1/4 TEa,WS/T-403-2012 and minimum imprecision derived from biological variation as quality specification.And an-alyzed the percentage of laboratories in meeting the quality standards.Results The TG,ALT,CK and TBil in more than 50% of the participated laboratories could meet the quality standards of the 1/3 TEa,1/4 TEa,WS/T-403-2012 and the low-est appropriate imprecision derived from biological variation.The Cl and Cr in more than 50% of laboratories couldn’t meet the all above quality standards.The Na and TCa in all laboratories couldn’t meet the quality standards of best imprecision derived from biological variation.The evaluation criterion for qualified items setted was that the variation coefficient in more than 80% laboratories was less than the quality standard.Thus,the percentage of the items which meet the lowest quality standard of biological variation and the all 21 items was the most (66.7%).While the percentage of the items which met the quality standards of the WS/T403-2012 and the best biological variation was the least (14.3%).Conclusion In short,the values of indoor variation coefficient of the 21 items in laboratories which participated the project of interlaboratory compari-sons of clinical chemistry indoor quality control data generally met the requirements.But some items had a little higher de-gree of dispersion.The laboratories should set the appropriate imprecision levels based on the detection capability and quality standards and improve the quality of examination through continuous efforts.

Objective: To understand the significance of serum TK1 level change during chemotherapy of patients with non-Hodgkin's lymphoma. Methods: The serum TK1 levels were detected in 37 patients with non-Hodgkin's lymphoma by enhanced chemiluminecence assay before chemotherapy and one day, four weeks after chemotherapy. Results: The changes of serum TK1 levels were significantly different at different stages of treatment (P<0.01). Conclusion: Our findings indicate that serum TK1 change might be of great value in evaluating the treatment outcomes and predicting recurrence of patients with non-Hodgkin's lymphoma.

Objective To review the management of impalpable testes. Methods Ninty-eight impalpable testes in 82 patients underwent surigcal exploration and all the cases were studied retrospectively. Results 17 testes were found on ultrasonography and 3 of 4 cases were found on ECT pre-operatively.Ninty-eight testes in 82 patients were explored.Eighty-five testes were found,52 in the inguinal canal,33 in the abdomen.Another 13 testes were absent.Ninty-two(94%) testes were found on inguinal exploration or limited laparotomy when necessary. Conclusions Inguinal exploration,with lower abdominal laparotomy if necessary remains the main form of management.If the testes could not be found still laparoscopy should then be performed.

Objective To investigate the therapeutic effects of matrine eye drops (MED) on experimental ocular inflammation in rabbits. Methods The effects of 0. 5 % and 1.0% MED, dexamethasone eye drops, saline solution were e-valuated in the rabbits with ocular inflammation induced by paracentesis of anterior chamber and capsicum tincture respectively. Results MED could significantly inhibited the increase of protein concentration in aqueous humor induced by anterior chamber paracentesis, and the anti - inflammatory effect of MED on capsicum - tincture - induced extra ocular and intraocular inflammation was similar to dexamethasone eye drops. There were no significant difference between 1.0% MED and dexamethasone eye drops. Conclusion MED has a good effect on experimental ocular inflammation in rabbits.

Objective To evaluate the value of the transnasal esophagogastrodudenoseopy(EGD)in clinical diagnosis and therapy.Methods The study includes 1000 patients referred for diagnostic and therapie EGD.All patients were received the examination after 2%lidocaine was administered into the uaris.We obtained biopsy specimens from 824 patients.Results Among the 1000 patients,971 were completed successfully by transnasal EGD,and the remaining was completed by classical transoral EGD.During the examination,we extracted the esopha6eal foreign body in two patients.and performed the pyloric stenting in one patient.Enteral feeding tubes were placed in 18 pafients.We found that the endoscopic manifestation is consistent with the histopathologic examination(the concordance rate is 84%).Conclusion The transnasal EGD has the goodish value in the clinical diagnosis and therapy.