AIM:To investigate the effects of propofol on ammonia-induced neocortical astrocyte aquaporin-4 expression in rats and its mechanism. METHODS:Astrocytes were separated from newborn Sprague Dawley rats. Glial fibrillary acidic protein,the specific protein of astrocyte,was labeled by cell immunofluorescence method. The purity of astrocyte achieved to 95% was considered to be used in the study. Grouping:cultured astrocytes were randomly divided into 5 groups (n=3):normal control group (N); ammonia-incubated for 24 h group (NH_4Cl-24 h); p38 antagonist SB203580 (10 μmol/L) pretreated group (SB); propofol (10 μmol/L) pretreated group (P); solvent (DMSO) control group (C). SB203580,propofol and DMSO were pretreated for 30 min before astrocytes were exposed to NH_4Cl. Cell morphology was assessed by light microscopy. The expression of aquaporin-4; p38 and p-p38 were detected by Western blotting. RESULTS:Astrocytes were found significant swelling when exposed to 5 mmol/L NH_4Cl in NH_4Cl-24 h group compared to control group. Pretreatment with propofol and SB203580 decreased astrocyte swelling. No significant change in total p38 in all groups was observed (P>0.05) by Western blotting analysis,while the levels of p-p38 and AQP4 in group SB and group P were significantly decreased compared to group C and group NH_4Cl-24 h (P<0.05).CONCLUSION:The expression of AQP4 is regulated by p38 pathway. Propofol pretreatment down-regulates aquaporin-4 expression through preventing the ammonia-induced p38 phosphorylation.

Childhood tuberculosis remains a serious public health threat which draws more and more attention.The unspecific symptoms or signs and the paucibacillary nature of childhood tuberculosis make it hard to diagnose which delays appropriate treatment and increases disease burden and mortality.Molecular methods such as polymerase chain reaction (PCR),real-time PCR,and DNA line probe based on mycobacterium tuberculosis specific nucleotide sequence will make a quick and reliable diagnosis of childhood tuberculosis.

Objective To study effect of traditional Chinese medicine chrysanthemum decoction on RSV infection after A549 cells chemotactic factor.Methods Subjects from The Second Hospital Affilated To Suzhou University in recent days, 64 cases of patients diagnosed with RSV effected pneumonia by clinical examination, RSV infection A549 cell model was established and the infected cells were cultured, chrysanthemums with TCM decoction and conventional antiviral observed its effect on RSV infection cell survival, compared two groups of cell SAP was determined by ELISA kit and the expression of RANTEs and chemokines MCP-1.Results Chrysanthemum, according to the results of in vitro cell culture studies traditional Chinese medicine decoction had the effect of directly inhibit the growth of virus replication, and there were some positive effects RSV infected A549 cells, MCP-1 and RANTEs expression or release significantly increased ( P<0.05 ) .Chrysanthemum Chinese medicine decoction and antiviral drugs after the intervention, the expression of MCP-1 and RANTES reduced significantly (P<0.05), but overall volume was still significantly higher than the normal. Conclusion Chrysanthemum Chinese medicine decoction can inhibit RSV infected A549 cells release chemokine RANTEs and MCP-1 to some extent, and then reduce the airway inflammation reaction of children.

AIM:To establish the method for quality control of Suhong Tongluo Tincture(eugenol and methylsalicylate). METHODS: Gas phase chromatogram approach was used to determine the content of eugenol and(methylsalicylate.) The chromatogram condition consisted of 10% PEG-20M,carrier was N2(99.999%),temperature of the entrance of the column was at 230(?C),temperature of the detector was at 250(?C);column temperature was at 70(?C)(4 min),3(?C)/min→90(?C)(4 min),then 13(?C)/min→200(?C)(3 min);0.5 ?L Sample quantity. RESULTS: The linear range of methylsalicylate was 2.41-14.21 ?g,r=0.999 7,the average recovery was (99.76%),RSD=(2.64%(n=6).) The linear range of eugenol was 2.43-14.34 ?g,r=0.999 96,the average recovery was (99.33%),RSD=2.47%(n=6). CONCLUSION: This method is simple,quick,accurate and reliable for the quality control of Suhong Tongluo Tincture.

Objective To analysis clinical significance of joint detection of serum Cys C,Hcy and mAlb in HDCP patients. Methods Selected 92 cases of HDCP patients in Guangji hosipital of Hezhou from Jun 2011 to Jun 2014 as observation group.They were divided into three groups (43 patients of gestational hypertension as group A,32 patients with mild pre-eclampsia as group B,17 patients of severe preeclampsia as group C)and chose 50 cases of healthy maternal the same period in the hospital as control group.Enhanced turbidimetric method for the determination of serum Cys C,Hcy,immune scatter-ing method for urinary mAlb,the results were analyzed.Results Observation group with the level of serum Cys C,Hcy and urinary mAlb was rise with the damage degree aggravating,group C rise significantly (F =4.567,P <0.001).Observation group serum Cys C,Hcy and abnormal detection rate of urinary mAlb level was rise at the same time renal damage degree aggravating,group C rise significantly (χ2 = 12.842,P <0.001).Three joint detection indices abnormal detection rate was significantly higher than that of single detection (χ2 =5.869,P <0.001),the detection sensitivity of 95.70% has significant advantage (χ2 =15.992,P <0.001).Conclusion The joint detection of renal function of serum Cys C,Hcy and mAlb in HDCP patients was better than the single detection,which is helpful for the early detection of HDCP patients with renal damage,it’s worthy clinical promotion.

Objective To learn how well these items of Hubei Province meet the quality standards of allowed imprecision. Methods Collected the indoor quality control data of median concentration levels from the laboratories which participated the project of interlaboratory comparisons of clinical chemistry indoor quality control data in Hubei Province.This paper was to analyze the variation coefficient of indoor quality control for 21 routine clinical chemistry examination items which were K,Na,Cl,TCa,P,GLu,Urea,UA,Cr,TP,Alb,TC,TG,ALT,AST,TBil,ALP,AMS,CK,LDH andγ-GT.The other objec-tive was to learn how well these items of Hubei Province meet the quality standards of allowed imprecision.Then took the 1/3 TEa,1/4 TEa,WS/T-403-2012 and minimum imprecision derived from biological variation as quality specification.And an-alyzed the percentage of laboratories in meeting the quality standards.Results The TG,ALT,CK and TBil in more than 50% of the participated laboratories could meet the quality standards of the 1/3 TEa,1/4 TEa,WS/T-403-2012 and the low-est appropriate imprecision derived from biological variation.The Cl and Cr in more than 50% of laboratories couldn’t meet the all above quality standards.The Na and TCa in all laboratories couldn’t meet the quality standards of best imprecision derived from biological variation.The evaluation criterion for qualified items setted was that the variation coefficient in more than 80% laboratories was less than the quality standard.Thus,the percentage of the items which meet the lowest quality standard of biological variation and the all 21 items was the most (66.7%).While the percentage of the items which met the quality standards of the WS/T403-2012 and the best biological variation was the least (14.3%).Conclusion In short,the values of indoor variation coefficient of the 21 items in laboratories which participated the project of interlaboratory compari-sons of clinical chemistry indoor quality control data generally met the requirements.But some items had a little higher de-gree of dispersion.The laboratories should set the appropriate imprecision levels based on the detection capability and quality standards and improve the quality of examination through continuous efforts.

Objective: To understand the significance of serum TK1 level change during chemotherapy of patients with non-Hodgkin's lymphoma. Methods: The serum TK1 levels were detected in 37 patients with non-Hodgkin's lymphoma by enhanced chemiluminecence assay before chemotherapy and one day, four weeks after chemotherapy. Results: The changes of serum TK1 levels were significantly different at different stages of treatment (P<0.01). Conclusion: Our findings indicate that serum TK1 change might be of great value in evaluating the treatment outcomes and predicting recurrence of patients with non-Hodgkin's lymphoma.

OBJECTIVETo analyze the changes in CIE L*, a*, and b* at cervical, body, and incisal sites after tooth bleaching by using a spectrophotometer.

METHODSSixty-seven intact and healthy maxillary central incisors were in-vestigated. These incisors were darker than A3 according to the Vita Classical shade guide. The CIE tooth shade parameters L*, a*, and b* were simultaneously recorded at three tooth areas (cervical, body, and incisal) with a spectrophotometer before and after tooth bleaching (35%H2O2 coordinating with Beyond whitening accelerator irradiating). The shade dif-ferential (DeltaE) was calculated. ANOVA, paired t-test, and Pearson correlation analysis were used for data analysis.

RESULTSThe efficacy rates of tooth bleaching were satisfactory, with 86.6%, 86.6%, and 85.1% in the cervical, body, and incisal sites, respectively. The average values of DeltaE were 5.09, 4.44, and 4.40 in the cervical, body, and incisal sites. Tooth bleaching significantly increased L* and significantly decreased a* and b* in all tooth areas (P < 0.01). The decreasing range of Deltab* was more than the increasing range of DeltaL* at the cervical site; opposite results were observed at the incisal site. A positive correlation was detected between baseline b* and DeltaE.

CONCLUSIONThe spectrophotometer could objectively evaluate the whitening effect of tooth bleaching at the different tooth sites. The tooth bleaching system (35%H202 coordinating with Beyond whitening accelerator irradiating) exerts powerful bleaching actions in most of the tooth areas investigated. The order of tooth bleaching effectiveness is cervicalbody>incisal. Yellow coloration is decreased mainly at the cervical site, and brightness was increased mostly at theincisal site. The effectiveness of tooth bleaching increases as the baseline b* value increases.

Gastrointestinal stromal tumors (GISTs) are common mesenchymal neoplasms in the gastrointestinal tract.The results of computed tomography play important roles in the diagnosis,treatment planning and follow up of GISTs. From August 2007 to January 2012,28 patients with GISTs were admitted to the Henan Provincial People's Hospital. Sixteen tumors showed extra-luminal growth,4 showed intra-luminal growth,7 involved both intra- and extra-luminal growth,and 1 showed extra-gastrointestinal growth.After administration of contrast media,9 tumors demonstrated homogeneous enhancement,18 showed heterogeneous enhancement and 1 showed non-obvious enhancement.The enhancement of tumors in arterial and venous phase was 20 Hu higher than that in plain scan.Angiogenesis was displayed in 6 tumors,and feeding arteries were observed in 3 tumors.

Objective] To explore the relationship between excessive internal heat in TCM and intestinal microflora ,provide scientific evidences for pathogenesis of excessive internal heat.[Method]With the research methods of clinical observation and literature review,analyze the characteristics of excessive internal heat,and collect research progress about intestinal microflora to find out the link.[Result] Head and facial tissue is more susceptible to injury,which is determined by the organizational characteristics. People suffering from excessive internal heat widely have problems of energy metabolism and mucosal immune disorders. Intestinal microflora plays an important role in energy production,conversion,storage and use.They can change the way that organizations use energy,and change AMPK phosphorylation to influence the energy metabolism of the body.Thus gut microflora imbalance may lead to disorders of energy metabolism.On the other hand,inflammatory mediators express abnormally in these people. Impaired immune function leads to inflammatory states,which causes imbalance of gut microflora.While intestinal microflora also can regulate intestinal mucosal immune response by adjusting related effective factors. [Conclusion]Excessive internal heat is closely related with intestinal microflora from the aspects of energy metabolism and mucosal immunity,which may provide new ideas to explore the pathogenesis of this disease.The researches based on intestinal microflora and diseases bring brilliant prospects of individual prevention and treatment for TCM and western medicine .