Objective To evaluate the satisfaction and usability of SoloStar(R),a prefilled pen for injecting insulin glargine ( Lantus(R),Sanofi ) in Chinese diabetic patients.Methods This was a 3-month,observational,multicenter,prospective registry study in adult diabetic patients who were ever insulin users or insulin naive.The SoloStar(R) was prescribed at physician's discretion.At baseline,patients were asked to evaluate their previous insulin pen if applicable.After 3-month SoloStar(R) use,the satisfaction and usability in using SoloStar(R) were assessed,and the evaluation from physicians was also collected.Results Total 1052 patients aged 56.5 years (27.6％ ≥65years),with mean diabetes duration 7.6 years,54.6％ male,94.1％ type 2 diabetes,6.5％ visual impairment,and 2.4％ dexterity impairment,were analyzed.Of these,488 patients (46.4％) had previously used insulin pen.Most patients (95.3％) rated SoloStar(R) assatisfied/very satisfied.Most patients planned to continue using SoloStar(R)(94.7％) and would recommend it to others(86.0％ ).Compared with previous insulin pen,most patients felt using SoloStar(R) became easier ( 57.3％ ) and more convenient ( 60.7％ ).63 physician questionnaires were analyzed,over 70％ physicians rated SoloStar(R) features as good/excellent,and 96.8％ physicians regarded training on SoloStar(R) as easier and quicker than other pens.Conculsion Most patients were satisfied/very satisfied with SoloStar(R) and most physicians rated SoloStar(R) as good/excellent,suggesting that SoloStar(R) was well accepted by patients and physicians in China.

Objective To investigate the mechanism of endothelial cell damage under insulin resistance state induced by high fat-feeding by observations of the effects of antioxidant N-acetylcysteine(NAC) on the blood glucose,lipids,the histological parameters of aorta,and the expression of intercellular adhesion molecules-1(ICAM-1),monocyte chemoattractant protein-1(MCP-1) and NF-?B/I?B? in aortic intima.Methods Forty-five Wistar male rats aged 5 to 6 weeks were randomly divided into 3 groups:control,high fat-feeding(HF) and HF plus NAC treatment(HF+NAC).Blood glucose,insulin,lipids,body weight,visceral fat content,histological change of aorta and the expression levels of ICAM-1,MCP-1 and NF-?B/I?B? were determined after the rats were fed for 11 weeks. Results(1)The body weight,ratio of visceral fat content over body weight,blood glucose,insulin,triglyceride,total cholesterol,LDL-C,and total FFA were significantly increased in HF group after 11 weeks feeding compared with control or HF+NAC(all P

Oxidative stress is a major contributor to the pathogenesis of diabetes and diabetic complications.Heme oxygenase(HO),an important part of the human defense system,has been recognized to have significant physiological function against oxidative stress.HO can be stimulated by oxidative stress and decompose into carbon monoxide,biliverdin and iron,all with important anti-inflammatory,anti-oxidative stress and anti-apoptosis effects.Overexpression of HO induced by hyperglycemia can protect cells(including islet cells,endothelial cells and monocytes) and tissues from oxidative damage.Increased HO metabolic products not only maintain the physiological function of islet cells, delay diabetes progression,but also regulate vascular,nerve and muscle function,so as to prevent diabetic complications.

In this study the role of acute administration of naloxone on LH pulse in normal adult men was evaluated. In 4 volunteers, blood samples were drawn at 10-min intervals for 10 hours both before and after administrating naloxone respectively for measurment of LH by RIA. The administration of naloxone resulted in an increase in LH pulse frequency from 5. 50?0. 50 to 8. 25?1. 09 (P

This article emphasizes the importance of medical communication and expounds how to strenthen medical communication by some principles and methods,like paying attention to and lov-ing patients,listenning carefully,explaining patiently and confidently,learning modestly and so on.

Objective To explore the effect of protease-activated receptor 1(PAR-1) activation on the expression of CXCR4 mR-NA of mouse endothelial progenitor cells (EPCs) and proliferation ,migration of EPCs .Methods Mouse EPCs were activated by different concentration of SFLLRN (one agonist of PAR-1) ,or transfected by small interfering RNA of PAR-1 .The expressions of PAR-1 and CXCR4 mRNA of EPCs were detected by fluorescent quantitative real-time PCR .Proliferation ,migration of EPCs were detected by MTT ,Transwell chambers respectively .Results The expressions of PAR-1 and CXCR4 mRNA in SFLLRN group were higher than that in other groups(P< 0 .05) .The expression of CXCR4 mRNA was highly positive correlation with PAR-1 mRNA(r=0 .991) .The proliferation ,migration of mouse EPCs were induced by activation of PAR-1 .Conclusion Activation of PAR-1 promotes cell proliferation and migration of mouse EPCs ,this effect may be depended on CXCR4 .

Objective To study the influence of hyponatremia on prognosis in hospitalized patients with chronic heart failure . Methods A total of 322 patients with chronic heart failure from Feb .2006 to Aug .2012 were retrospectively reviewed and random-ly divided into hyponatremia group(n=161) and normal serum sodium group(n=161) .The clinical data of the two groups were compared .Results There were significant difference between the two groups in the BNP levels ,length of stay ,hospital mortality and readmission rates(P<0 .05) .Serum sodium concentration in hyponatremia group was decreased with the decrease of cardiac function(P<0 .05) ,BNP levels was elevated with the decrease of blood sodium level (P<0 .05) ,days of hospitalization and hospital mortality were increased with the decrease of cardiac function (P< 0 .05) .Conclusion Patients with heart failure combined hy-ponatremia have poor cardiac function ,higher in-hospital mortality and readmission rates and longer hospital stay .

Objective To explore the protective mechanism of metformin in the process of cultured human umbilical vein endothelial cells (HUVECs) injury induced by palmitic acid (PA). Methods HUVECs were cultured in the media of blank,PA,metformin,or PA combined metformin for 24 h,48 h,72 h respectively. Expressions of monocyte chemoattractant protein-1 (MCP-1) and intercellular adhesion molecule-1 (ICAM-1) were determined by RT-PCR and the expressions of ICAM-1,nuclear factor kappa B p65 (NF-?B p65) and phospho-I?B? was evaluated by Western blotting. Results Significant increase of the expressions of MCP-1 and ICAM-1 was found in PA group compared with in the control group (P

Objective:To investigate the role of saturated free fatty acids (SFFAs) in the pathogenesis of atherosclerosis by determining the effects of FFAs on diacylglycerol (DAG) synthesis and protein kinase C (PKC) activity in cultured bovine aortic smooth muscle cells. Methods:Bovine aortic smooth muscle cells, cultured in vitro, were treated with 200 ?mol/L SFFAs (including palmitate and stearate) and unsaturated FFAs (including oleate and arachidonate) separately for 24 h. Then the cells were lysed and the lipids were treated with lysis buffer and separation buffer. DAG level and PKC activity were calculated through measuring the radioactivity of 32P-PA by liquid scintillation counting (1 cpm=6.17?108 Bq). Results:Incubation of the cells with saturated FFAs(palmitate or stearate, 200 ?mol/L, for 24 h) induced significant increase in DAG concentrations (927.2 and 1 533.3 pmol/106 cells by palmitate and stearate, respectively) compared with that of the control cells (190 pmol/106 cells,P

Objective:To study the diagnostic value of combined detection of IAA,ICA and GADA in the classification of diabetes mellitus.Methods:30 cases of patients with type 1 diabetes who were treated in our hospital from June 2015 to June 2016 were selected as A group,60 cases of patients with type 2 diabetes were selected as B group,50 cases of healthy people were selected as C group.The IAA,ICA and GADA of the three groups were detected by ELISA,and the positive rate of the three groups were compared.Results:The fasting glucose of A group was (10.12± 3.68) mmol/L,B group was (11.23± 3.26) mmol/L,A group and B group were significantly higher than that of C group (P＜0.05),but there was no significant difference between A group and B group (P＞0.05);the positive rates of GADA,ICA and IAA in A group and B group were significantly higher than those in C group (P＜0.05),and the positive rates of GADA,ICA and IAA in A group were significantly higher than those in B group (P＜0.05);the sensitivity and specificity of combined detection of IAA,ICA and GADA in type 2 and type 1 diabetes mellitus were significantly higher than that in the single test (P＜0.05).Conclusions:The combined detection of IAA,ICA and GADA has a high diagnostic value in the classification of diabetes mellitus,which is worth clinical application.