To investigate the solubility and thermodynamics parameters between brucine and β-cyclodextrin polymer (β-CDP) under the condition of different temperature and pH solution. Methods:The solubility effect ofβ-CDP was performed by the phase-solubility method and the inclusion constant ( Kc ) and thermodynamic parameters were calculated. Results:The Kc at the differ-ent temperature of 25℃,37℃ and 45℃ was 88. 38,349. 29 and 641. 79 L·mol-1 , respectively, and that at the pH of 4,6. 8 and 10 was 490. 66,111. 85 and 58. 86 L·mol-1 , respectively. The solubility of brucine was increased linearly with the increase of β-CDP concentration in the AL-type phase solubility curve. The inclusion constant and solubilization of β-CDP were both higher under acidic conditions. Conclusion:The solubility of brucine in water can be improved byβ-CDP, and brucine andβ-CDP can form stable inclu-sion complexes with molar ratio of 1∶1. The inclusion compound can form spontaneously (ΔG<0), and high temperature and low pH value can enhance the formation of the inclusion compound.

AIM: To elevate the stability of the volatile oil in Jiufen Cataplasm (Semen strychni,Herba ephedrae,Oilbanum and Myrrha) with the help of inclusion with ?-Cyclodextrin. METHODS: The quality of the volatile oil in Jiufen Cataplasm before and after stability test was examined by thin layer chromatography and differential thermal analysis and thermogravimetric analysis. RESULTS: The combination of volatile oil and ?-cyclodextrin could form stable inclusion compound that helped constant in before and after inclusion. CONCLUSION: The process of enclosure with ?-CD can keep the active components of the volatile oil in Jiufen Cataplasm.

Objective To perform comparative proteomic analysis of human ovarian cancer cell lines for detecting platinum-resistance associated proteins.Methods The total proteins of two sensitive (SKOV3 and A2780)and four resistant(SKOV3/CDDP,SKOV3/CBP,A2780/CDDP and A2780/CBP) human ovarian cancer cell lines were separated by two-dimensional gel electrophoresis(2-DE).The differentially expressed proteins were analyzed using image analysis software,stained with Coomassie Brilliant Blue,then identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS)and database searching.The mRNA and protein levels of the differentially expressed protein which was most significant in all of the four resistant cell lines were validated by RT-PCR and western blotting,respectively.Results Five proteins were found to be significant in four cell lines. Annexin A3 and destrin were up-regulated and nicotinamide-adenine dinucleotide phosphate(NADP)- dependent isocitrate dehydrogenase 1 was down-regulated in all the four resistant samples.Glutathione transferase omega 1 had an increased expression in the other three resistant cell lines except for SKOV3/CBP in which its expression was not changed.However,cofilin 1 represented a different trend.In the two resistant sublines of SKOV3,eofilin 1 had a down-regulation,but it had an up-regulation in the cell lines induced from SKOV3.The expression of annexin A3 was up-regulated by 3-20 fold and the results of RT- PCR and western blotting showed complete consistency with that by 2-DE.Conclusions Proteomic techniques are useful to the identification of the resistance-associated proteins in ovarian cancer platinum- resistant cell lines and five candidates have been found.The five differential proteins might become hopeful candidate biomarkers for resistance.

Animals ; Dementia, Vascular ; drug therapy ; physiopathology ; Hippocampus ; metabolism ; physiopathology ; Learning ; drug effects ; Male ; Memory ; drug effects ; Nootropic Agents ; pharmacology ; therapeutic use ; Proto-Oncogene Proteins c-bcl-2 ; genetics ; metabolism ; Pyrrolidinones ; pharmacology ; therapeutic use ; Rats ; Rats, Sprague-Dawley

Acrylamide ; analysis ; Air Pollutants, Occupational ; analysis ; Chromatography, High Pressure Liquid ; methods ; Workplace

China ; Clinical Laboratory Techniques ; Laboratories ; statistics & numerical data ; Occupational Health ; Quality Control

Humans ; Indicators and Reagents ; supply & distribution ; Occupational Health

Schistosomiasis is a neglected tropical disease which is socioeconomically devastating and a significant cause of morbidity in endemic countries or regions. Some countries and regions have brought down the prevalence of schistosomiasis through positive prevention and control programs. However in the past few years with the social and economic development and globalization re?emergence and spread of schistosomiasis led to a growing concern that new endemic areas may occur. This article analyzes the epidemiological situation and the strategies to control schistosomiasis in China and African countries.

Neuromyelitis optica ( NMO) is an idiopathic autoimmune inflammatory disorder of the central nervous system ( CNS) that predominantly affects the optic nerves and spinal cord.It is especially common in Asia. An association between NMO and other autoimmune diseases has been frequently reported, which is different from multiple sclerosis( MS) ,this phenomenon may due to different genetic background between these two diseases.There are reports on different populations to make this clear,which showed human leukocyte antigen( HLA) class II distribu-tion was unique entity different between NMO and MS or healthy controls,this would be a great help to make clear the mechanism underlying NMO pathogenesis.

OBJECTIVE:To construct the structure equation model for the burden of chronic disease inpatients in primary hos-pital before and after the implementation of zero price policy,and analyze the changes of burden factor loading. METHODS:6 pri-mary hospitals were randomly selected in Danyang,Jiangsu province. The data of hospitalization expenses for chronic diseases(hy-pertension,diabetes,bronchitis)were collected before and after the implementation of zero price policy. Using drug cost,nursing fees,inspection fees and treatment fees as independent variables,hospitalization burden as latent variable,SPSS and AMOS 24.0 software were adopted to establish the model. RESULTS:The burden factor loading of inpatients changed greatly before and after the implementation of zero price policy. χ2=24.586,χ2/df=1.446, RMSEA=0.019,GFI=0.995,AGFI=0.989,CFI=0.988,NFI=0.963 manifested good model fitting. Factor loading of drug cost increased greatly,indicating the burden of inpatients was reduced after the implementation of zero price policy. Factor loading of inspection fees and treatment fees increased significantly,the charac-terization effects of them to the burden of inpatients were enhanced. Factor loading of nursing fees was the lowest,and it had the weakest effects on the burden of inpatients,of which the government should enhance the regulation. CONCLUSIONS:Established model has good reliability and validity. It can reflect the change of burden factor loading of inpatients before and after the implemen-tation of zero price policy.