Objective To investigate the distribution of pathogenic microbes in blood culture and analysis of drug resis-tance of common pathogens in children with acute leukemia.Methods BACTE-FX system made by BD company was applied for the blood culture of children with acute leukemia. API or VITEK-2 Compact bacterial identiifcation system was used for iden-tiifcation of bacteria. Antimicrobial susceptibility testing was performed by disk diffusion method.Results Among 6400 blood culture samples in children with acute leukemia, 713 cases were positive at the rate of 11.1%. Among 655 strains ifrst isolated from the patient, Gram-positive bacteria were 52.5% (344/655) and dominated byStaphylococcus. Strains resistant to vancomy-cin and linezolid were not found. 278 strains (42.5%) were Gram-negative bacteria , andS.marcescens andE.coli were dominat-ed. Gram-negetive bacteria showed high antibacterial activity to imipenem with the sensitive rate of 100%. 33 strains (5%) were fungi.Conclusions Staphylococcus,S.marcescens, and E.coli were the most important bacteria in blood culture of children with acute leukemia. We can offer effective help for clinical treatment with the susceptibility testing.

New military transformation and modernization of Chinese army brings new challenges to medical equipment of PLA. This paper introduces challenges by development of science and technology, flourish of economy, medical reform and military transformation.

Objective:Examining the difference in the protein profiling of endometrium between the blastocyst-adhering side and the side without blastocyst adhering,in order to study the asynchrony of blastocysts adhesion between two uteri.Methods:The endometrium with and without blastocyst adhering from the mice pregnant for 5 days were analyzed by two-dimensional gel electrophoresis and imaging analysis.Results:The number of matched protein spots between two gels was up to 82.5%.760 and 720 protein spots with a molecular mass between 14.0～75.4 and isoelectric points (pI)from 4～7 were catalogued from silver-stained gels of the blastocyst-adhering endometrium and the endometrium without blastocyst adhering respectively.At least 60 proteins were up-regulated or down-regulated by over two folds between two groups.Conclusion:the different expression of protein profile may be a cause underlying the the asynchrony of blastocysts adhesion between two uteri.

AIM: To investigate the effect of exercise training(EX) on the arterial baroreflex and possible mechanisms in rats with chronic heart failure(CHF).METHODS: Experiments were carried out in four groups: EX-CHF,CHF,EX-sham,and sham. CHF was induced by left coronary artery ligation,and EX consisted of 4 weeks of treadmill running. Baroreflex function,plasma angiotensin II(Ang II) and central AT1 receptor expression were determined.RESULTS: (1) The average slope and maximal gain of the baroreflex curve in CHF rats were lower than those in sham rats(P<0.01). EX significantly enhanced baroreflex parameters in CHF group but not in sham group.(2) EX decreased plasma Ang II in CHF rats ［(137±27)ng/L vs (263±55)ng/L,P<0.01］,but had no significant effect on plasma Ang II in sham rats ［(75±17)ng/L vs (92±21)ng/L］.(3) Expression of AT1R protein in the PVN of CHF rats was higher than that in sham rats(1.20±0.21 vs 0.70±0.14,P<0.01). EX reduced AT1R protein level in CHF rats(0.90±0.13),but had no impact in sham rats(0.60±0.16).CONCLUSION: EX restores the attenuated arterial baroreflex function in CHF rats,involving in decrease of plasma Ang II and downregulation of AT1R in the PVN.

Objective To investigate the effect and mechanism of the icaritin on the human cronic myeloid leukemia K562 cells. Methods The K562 cells at logarithmic growth phase were divided into the control group and the icaritin group. The cells in the control group were normally treated and the cells in the icaritin group were incubated with 8 μmol/L icaritin. Methyl thiazolyl tetrazolium (MTT) method and flow cytometry were used to examine the proliferation and apoptotic changes in the two groups after incubation for 72 h, respectively. Gene expression of p85 and Akt were detected by RT-PCR. The protein changes of p85, Akt, p-p85, p-Akt cleavage-caspase-3 and caspase-3 were detected by Western blot. Results Compared with the control group, the proliferation rate of K562 cells in the icaritin group was significantly decreased (P<0.05), however the apoptotic rate of K562 cells and the expressions of p-p85, p-Akt, cleavage-caspase-3 in the icaritin group were significantly increased (all P< 0.05), but the expressions of p85 mRNA, Akt mRNA and caspase-3 protein had no difference (all P> 0.05). Conclusion Icaritin could induce the proliferation and promote the apoptosis of K562 cell, and its mechanism may be achieved through activating the PI3K-Akt signal transduction pathway.

AIM:To determine the effect of reactive oxygen species on the baroreflex and to investigate the intracellular mechanism responsible for baroreflex dysfunction in the heart failure state.METHODS:In the rat model of cardiomyocytes infarct induced heart failure,baroreflex function was evaluated by measuring the relationship between renal sympathetic nerve activity(RSNA)responses and change of blood pressure by intravenous injection of nitroglycerin and phenylephrine.Alteration in baroreflex function was measured under the different reactive oxygen species(ROS)level induced by intracerebroventricular administration of several chemicals.RESULTS:(1)The range of RSNA response,average slope and maximum gain of baroreflex function curve were(92.2?9.9) mmHg,(0.07%?0.01%)/mmHg and(1.20%?0.10%)/mmHg,respectively,in CHF rats,which were significantly lower than those in sham rats(65.6?7.4) mmHg,(0.13%?0.02%)/mmHg and(3.00%? 0.20%)/mmHg(P

As the people's life quality improved, more and more attention has been paid to indoor air pollution. The SBS (sick building syndrome) resulting from indoor air pollution has become an unavoidable problem in the modern life. Indoor air pollution has been listed as one of the five most dangerous environmental factors to human health. The present paper mainly analysed the prominent indoor air pollutants such as formaldehyde, radon, ammonia, nitrogen oxides and VOCs, which affect indoor air quality, and also pointed out the research direction and the high lights in this field in future.

Colorectal cancer has the propensity to develop liver-dominant metastases.In some of these patients,the liver is the only site of metastatic disease.Thus,surgical resection is the mainstay of treatment for liver-only colorectal cancer metastases,which can produce long-term survival in selected patients,but only 10%-20% of patients are suitable for a surgical approach.In this paper we introduced how to transfer the inoperable colorectal liver metastases to operable colorectal liver metastases through improved chemotherapy with /without molecular target therapy and multidisciplinary treatment approaches,and the results of radiofrequency ablation(RFA),which can also produce long-term survival in a subset of patients with colorectal liver metastasis or prolong survival duration.

Objective To summarize the modernization of information demand of clinical teaching and management of nursing interns, evaluate the effect of development of the nursing intern teaching management information platform in the clinical effect. Methods Through literature retrieval, inductive method, on the basis of the needs analysis of nursing interns, clinical teachers, Nursing Education Center personnel a total of 41 people were divided into 5 groups with 5-7 people each. Brainstorming method was used to build the initial module and design the questionnaire and the interview outline. The clinical teaching teacher questionnaire was investigated in 101 part and in-depth interviews. Results The recovery of 100 valid questionnaires, the effective questionnaire 99.0%(100/101) and in-depth interviews with 15 excellent clinical teachers, the final 7 first level indices and 55 two level indices; finally running test platform development and 6 months, again the clinical teacher interviews, trial platform had a satisfactory result. Conclusion The nursing intern teaching management information platform is helpful to improve the teaching quality of nursing interns and management efficiency.

Urinary albumin is a biomarker for kidney damage and is widely applied in clinical practice.With more evidence on urinary albumin, it is also recognized as a risk factor for cardiovascular disease and all-cause mortality.This paper focuses on thecurrent status of pre-analytical, mid-analytical and post-analytical process and discusses the progress in standardization of measurement for urinary albumin and the issues which call for the attention of clinical laboratorian.