Objective:To understand the attitudes of medical students to public elective courses of humanistic quality and the existing problems. Method:Questionnaires were used in 1 ~5 grade students in randomly selected five medical colleges in Guizhou. Results:Total 1000 questionnaires were sent out, 957 were received, the recovery rate was 95. 7%;817 valid questionnaires, the effective rate was 81. 7%. 75. 56% of the students supported for medical colleges and uni-versities setting up humanistic quality public elective courses, but 24. 44% had negative attitude. Conclusions:It's nec-essary to make efforts to improve the mind medical student's humanistic quality. From the perspective of serving students, school should improve medical college students' humanistic quality from various aspects.

OBJECTIVE:To establish the quality standard for Anti-virus chewable tablet. METHODS:TLC was used for the qualitative identification of Forsythia suspensa,Anemarrhena asphodeloides and Pogostemon cablin in Anti-virus chewable tablet, and HPLC was conducted to determine the content of forsythin in F. suspensa. The column was Diamonsil C18 with mobile phase of acetonitrile- water (20∶80,V/V) at the flow rate of 1.0 ml/min,the detection wavelength was 230 nm,column temperature was 25℃,and injection volume was 10μl. RESULTS:The test sample and the reference sample displayed same color spots on the cor-responding position in TLC diagram. The linear range of forsythin was 0.053 5-2.675 μg(r=0.999 9);RSDs of precision,stabili-ty and reproducibility tests were no more than 1.74%;average recovery was 98.94%(RSD=1.84%,n=6). CONCLUSIONS:The method is simple,rapid,accurate,reliable and reproducible,and can be used for quality control of Anti-virus chewable tablet.

Taking 98th Hospital of PLA as an example, based on analyzing current existing problems in hospital data center, the pa-per introduces the plan of hospital cloud computing data center construction based on VMware virtualization technology, describes the de-sign principle, overall architecture, implementation steps and application effect, points out that the application of cloud computing tech-nology will become the construction and development direction of hospital data center.

Objectives: To reveal the possible role and mechanism of disc-facet angle in the occurrence and progress of Hirayama disease by analyzing the correlation of clinical appearance and the variation of disc-facet angle in Hirayama disease. Methods: 45 patients with Hirayama disease treated in the orthopedic de-partment of Peking University Third Hospital from October 2006 to January 2012 were reviewed. There were 44 males(97.8%) and 1 female(2.2%) with an average age of 19.33±3.89(range 13-37) years old. The onset age was 16.33±2.73 (range 10-27) years old and the duration of history was 35.64±23.24 (range 1 to 120) months. The disc-facet angle of C3-T1 was measured on PACS system. An extension line was made along the upper edge of objective vertebral body on the CT scan sagittal reconstruction image. An extension line was made along the joint surface of upper articular process of objective vertebra on the sagittal plane which crossed the midpoint of the facets. The blunt angle between two lines was the disc-facet angle. The cases were divided into unilateral affect and bilateral affected group according to history, spinal cord function and spinal cord lesion level. The correlation of above data and the variation of disc-facet angle were analyzed. Results: The duration of history in unilateral affected patients was significantly shorter than that of bilateral affected patients (P<0.05). The duration of history in sequential onset patients was significantly shorter than that of simultaneous onset in bilateral affected patients (P<0.05). The JOA score 17 and Chinese score 40 in unilateral affected patients were higher than those of bilateral affected patients. The C3-C7 disc-facet angle of affected side was bigger than that of normal side in unilateral affected patients, and C5, C6 had statistically significant difference (P<0.05). The spinal cord lesion level was mainly at C5 and C6 in unilateral affected group. The disc-facet angle had no difference on both sides in bilateral affected patients but the figures at C4 and C5 level were bigger than that of normal side in unilateral affected patients. The spinal cord lesion level was mainly at C4 and C5 in bilateral affected group. The stability of upper cervical spine segments was even worse in unilateral affected group and resulting in higher level of spinal cord lesion comparing with bi-lateral affected group. Conclusions: The disc-facet angle of C4, C5 is bigger in bilateral affected patients and may be correlated with higher level spinal cord lesion and more severe clinical symptoms. The C5, C6 disc-facet angle of affected side is significantly bigger than that of normal side in unilateral affected patients. This may be the key factor of spinal cord lesion mainly at C5 and C6 level on the same side.

Objectives: To study the morphological difference of luscka joints between Hirayama disease patients and non-Hirayama disease patients on CT scan and to provide a new possible mechanism of Hirayama disease. Methods: 32 patients(all males) with a mean age of 19.4±4.1(range 16-37 years) and with Hirayama disease were treated in our hospital from October 2006 to January 2012, the mean course of disease was 31.7±23.7 months(range, 1-120 months). 32 patients(all males) with a mean of age was 19.1±4.3 (range, 12-26 years) suffering from acute neck pain and having no Hirayama disease were reviewed as control. Both groups showed no age related difference. From the cervical CT coronal plane reconstruction images which passing through the transverse foramen center of C3-C7 in GE-PACS system, the following data were measured in both sides: ①The width of the uncinate process base: the distance between inner and outer margin of the uncinate process at the upper edge of the vertebral body. ②The height of the uncinate process:the vertical distance from the top of the uncinate process to the upper edge of the vertebral body. ③The distance between two uncinate processes: the distance between the tips of the bilateral uncinate processes. ④The inclination angle of the uncinate process: the angle between the uncinate process and the upper edge of the vertebral body. ⑤The inclination angle of the inferior endplate: the angle between the uncinate process:the vertical distance from the top of the uncinate process to the upper edge of the vertebral body. Results:There were no significant side-related differences on the width of the uncinate process base, the height of the uncinate process, the distance of the uncinate process, the inclination angle of the uncinate process and the inclination angle of the inferior endplate at the same segment from C3 to C7 in Hirayama disease patients(P>0.05). However, differences were found on the height of the uncinate process and the distance between two uncinate processes of C3-C7 (P<0.05), C6 and C4 had the highest and lowest height of the uncinate process as C6>C5>C7>C3>C4. The distance of the uncinate process gradually increased from C3 to C7. There were no significant differences on the width of the uncinate process base, the inclination angle of the uncinate pro-cess and the inclination angle of inferior endplate(except for C3) of C3-C7(P>0.05). Then, using the mean value of the left and right sides as the width of the base of uncinate process, the height of uncinate process, and calculating the sum of inclination angle of the uncinate process, the sum of inclination angle of inferior endplate of the upper vertebra and the difference between the sums (the sum of inclination angle of uncinate process - the sum of inclination angle of inferior endplate of the upper vertebra), then calculating the mean value and standard deviation. Compared with the non-Hirayama disease patients, there were no significant dif-ferences on the uncinate process base, the distance of the uncinate process and the sum of inclination angle of the uncinate process at the same segment of Hirayama disease patients (P>0.05), while the height of the uncinate process and the sum of inclination angle of uncinate process of Hirayama disease patients were sig-nificantly smaller than those of the control group, respectively(P<0.05), and the differences between the sums were larger than those of the control group (P<0.05). Conclusions: Hirayama disease patients may possess a dysplasia in the luscka joint, manifesting the nonuniform development of the uncinate indicators. Lower unci-nate process and smaller inclination angle of inferior endplate of the upper vertebra are common, The conse-quential cervical instability may play a significantly important role in the pathogenesis and progress of Hi-rayama disease.

This paper introduces a kind of design scheme with the application of M-bus to concentrated oxygen supply data acquisition and measurement.The method to integrate all kinds of consumable equipments and products of different companies is also presented.It is proved that the data acquisition system by M-bus can be applied to concentrated oxygen supply data acquisition and measurement in hospital.

Objective To evaluate denervation changes [fibrillation potentials (fib) and positive sharp waves (psw)] in thoracic paraspinal muscles as diagnostic aids in amyotrophic lateral sclerosis (ALS). Methods The fib and psw of thoracic paraspinal muscles,the motor unit potential of sternocleidomastoid muscle were detected in 50 patients who had been identified as having ALS.Age and sex-matched,30 normal subjects and 30 patients who suffered radicular disease in cervical or lumbosacral region were also detected in thoracic paraspinal muscles for finding fib and psw serving as controls. Results In ALS group,a vast of fib and psw were found in thoracic paraspinal muscles in 41 of 50 ALS patients (82%),they were not found with sternocleidomastoid muscle,but the motor unit potential of this muscle was shown having long-duration and high-amplitude in 48 of 50 ALS patients (96%),which were coincided with the chronic neurogenic changes.6 of 50 patients had denervation changes of tongue muscles,3 of them found fib and psw.2 of 30 patients who suffered from radicular diseases were found with a few of fib and psw changes in thoracic paraspinal muscles.None of these symptoms were found in normal subjects.Conclusion A vast of fib and psw in thoracic paraspinal muscles should represent a sensitive tool for diagnosis of ALS.

Objective: To establish a method for determination of mercury (Hg) and arsenic (As) contents in biological samples. Methods: Biological samples containing Hg were pretreated with digestive agents of HNO3-H2SO4 and that of As with HNO3-HClO4 and HNO3-H2O2 under the pressure of 0.5 MPa,1.0 MPa and 1.5 MPa respectively heated for 2 minutes. And then the concentrations of Hg or As were determined by intermittent flow hydride generation-atomic spectrophotofluorimetry. Results: Recoveries of Hg and As were 97.3 %～99.1 %and 99.4 %～105.7 %respectively. Conclusion: The method is simple, rapid, accurate and sensitivite and with good reproducibility and is suitable for the determination of Hg and As contents in biological samples.

Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Longitudinal Studies ; Male ; Neoplasm Staging ; Neuroblastoma ; diagnosis ; pathology ; therapy ; Treatment Outcome

Objective: Our aim was to analyze the relationship between the concentration of some minerals in saliva and periodontitis.Methods:Twenty-five patients with adult periodontitis (AP group), 25 age and sex matched healthy people(N group) were selected. Unstimulated whole saliva samples were collected and qualified for the concentration of TCa,P,Mg2+, Na+,K+, and Cl-.　Clinical parameters on periodontitis were also assessed for the patients. All the aforementioned indices were measured repeatedly at the end of the fourth week after initial therapy. Results:The clinical condition of the patients improved significantly after treatment. The level of saliva Ca elevated before and after treatment in AP group than that in N group. Before treatment, the concentration of Na+ also increased significantly, but it decreased to normal level after initial therapy. The findings also indicated the positive relationship between the concentration of salivary Na+ and pocket probing depth as well as gingival index before treatment.Conclusion:The elevated level of saliva Ca is a sign of periodontitis and may help the occurrence and progress of periodontitis. The level of saliva Na+ may indicate the pocket depth and degree of gingivitis.The saliva Na＋ is closely associated with the clinical condition of periodontitis.