Objective Hes5 is one of the critical effectors of the Notch pathway and its expression Call inhibit neuronal differentiation.Which protein expression is influenced in cell differentiation by HeS5 remains to be determined.Methods Experiments were designed to study changes in protein expression in the subventricular zone(SVZ)of the HesS knockout mice at the postnatal day 8 using the new method of proteomic isobaric tags for relative and absolute quantification(ITRAQ),and confirmed expression of the up-gnlated and down-regulated proteins by Western blotting.Results Nineteen proteins showed differences in expression levels in the SVZ of Hes5 knockout mice.When compared tO the wild type mice,expressions of 11 proteins in the knockout mice were up-regulated,and the differentially expressed proteins showed ratios of knockout/widtype between 1.20 and 1.32.Expressions of 9 proteins were down-regulated,with their ratios were between 0.77 and 0.83.The upregulated proteins have been shown to be involved in neurogenesis,cell adhesion,migration and signal transduction.The downregulated proteins have been mainly shown to be involved in growth inhibition and cytoskeleton.Westem bloning analysis further confirmed the expression of proteins from iTRAQ.Conclusion Our findings suggest that Hes5 may influence cell differentiation by regulating expression of some proteins involved in cell growth and migration,and its cytoskeleton.

Objective Hes5 is one of the critical effectors of the Notch pathway and its expression can inhibit neuronal differentiation.Which protein expression is influenced in cell differentiation by Hes5 remains to be determined. Methods Experiments were designed to study changes in protein expression in the subventricular zone(SVZ) of the Hes5 knockout mice at the postnatal day 8 using the new method of proteomic isobaric tags for relative and absolute quantification(iTRAQ),and confirmed expression of the up-gulated and down-regulated proteins by Western blotting. Results Nineteen proteins showed differences in expression levels in the SVZ of Hes5 knockout mice.When compared to the wild type mice,expressions of 11 proteins in the knockout mice were up-regulated,and the differentially expressed proteins showed ratios of knockout/widtype between 1.20 and 1.32.Expressions of 9 proteins were down-regulated,with their ratios were between 0.77 and 0.83.The up-regulated proteins have been shown to be involved in neurogenesis,cell adhesion,migration and signal transduction.The down-regulated proteins have been mainly shown to be involved in growth inhibition and cytoskeleton.Western blotting analysis further confirmed the expression of proteins from iTRAQ.Conclusion Our findings suggest that Hes5 may influence cell differentiation by regulating expression of some proteins involved in cell growth and migration,and its cytoskeleton.

Objective: To study the effects of dietary n-6/n-3 PUFA ratio on T lymphocyte functions in mice. Methods: 96 male BALb/c mice were randomly divided into 8 groups with dietary S:M:P 1:1.5:3.7 or 1:1.5:1, respectively. The n-6/n-3 PUFA ratio was 1, 7.5, 15, 30 respectively in each S:M:P diet. Each diet contained 70.0g/kg lipid,and different n-6/n-3 PUFA ratios by mixing different oils. At the end of 12w, all mice were killed and blood was collected. The spleen T lymphocyte proliferation, IL-2 production and serum MDA were measured. Results: When dietary S:M:P was 1:1.5:3.7, the proliferation of spleen lymphocytes and IL-2 production from mice with dietary n-6/n-3 ratio 1 were suppressed (P

Objective To evaluate the bridging role of non-bioartificial liver for patients with chronic severe hepatitis B awaiting liver transplantation. Methods The non-bioartificial liver support was used to treat 9 hepatitis B patients with advanced stage liver failure waiting for liver donor in order to correct physiological disturbances and bridge to orthotopic liver transplantation (OLT). Results When the patients were treated with non-bioartificial liver support, the concentrations of serum total bilirubin and total bile acid were decreased dramatically (P

Objective To assess whether echocardiographic parameters of M-mode,pulsed-wave Doppler and tissue Doppler imaging which were usually used for selecting cardiac resynchronization therapy (CRT) candidates could predict CRT responders.Methods Twenty-nine patients with NYHA class Ⅲ or Ⅳ heart failure,left ventricular ejection fraction(LVEF)≤35%,QRS≥130 ms were enrolled.All of them underwent biventricular pacemaker implantation.The septal-to-posterior wall motion delay(SPWMD) from M-mode recording,and the standard deviation of time to peak systolic velocity(Ts-SD) were used to evaluate intraventricular dyssynchrony.The interventricular mechanical delay (IVMD) by pulsed Doppler echocardiography was calculated by subtracting the right ventricular preejection intervals (RV-PEI:the onset of the QRS on the electrocardiogram to the onset of pulmonary outflow) from the left ventricular preejection intervals (LV-PEI:the onset of the QRS on the electrocardiogram to the onset of aortic outflow).At least 15 % reduction in left ventricular end-systolic volume(LVESV) at the 6-month after CRT was defined as responder.Results In the 29 patients,there were 20 (68.97%) responders.The area under the receiver operating curve(ROC) of SPWMD,Ts-SD,LV-PEI,RV-PEI and IVMD to predict CRT responders was 0.448 (95% CI 0.184-0.712,P＞0.05),0.557 (95% CI 0.317-0.798,P＞0.05),0.479 (95% CI 0.186-0.773,P＞0.05),0.568 (95 % CI 0.272-0.863,P＞0.05) and 0.411 (95% CI0.136-0.687,P＞0.05),respectively.Conclusions M-mode echocardiography,pulsed Doppler and tissue Doppler imaging failed to predict CRT responders.New echocardiographic techniques including threedimensional echocardiography and speckle tracking image will be investigated,and may improve the predictive power.

Objective To observe the anatomical and biomechanieal properties of the forearm in terosseous membrane (IOM), providing the scientific theoretical basis for the diagnosis and treatment of the IOM injury. Methods Ten radius-IOM-ulna structures (left for 5 and right for 5) were harvested from 5 fresh-frozen adult cadavers to measure the length, width and thickness of the tendinous part. Then the tendi-nous part with its radial and ulnar insertions were isolated, embedding the proximal part of the radius and the distal part of the ttina in commercially available dental base acrylic resin powder. The embedded speci-mens were mounted on MTS 858 testing machine using a 10 000 N load cell for all tensile tests. The speci-mens were tested at a displacement rate of 50 mn/min until failure. The load-displacement curve was de-scribed by the computer while the maximum load and stiffness were recorded. Results The IOM consisted of three components: the tendinous part, the membranous part, and the dorsal oblique accessory cord. The IOM was fiat in the neutral position, while it flexed during the pronation and supination. Six specimens ob-tained the tear of tendinous portion at a maximum load of (1021.50±250.13) N. The stiffness was (138.24±24.29) N/mm with displacement to failure of (9.77±1.77) mm. Other 4 specimens failed by fracture through the ulnar fixed site before there was failure of the IOM at a maximum load of (744.40±109.85) N. The stiff-ness was (151.17±30.68) N/mm with displacement to failure of (6.51±0.51) ram. Conclusion The IOM has stiffness comparable to the ligament between the radius and the ulna and play an important role for the maintenance of longitudinal stability of the forearm. These results can provide objective criteria for the eval-uation of reconstructive methods.

Objective To investigate the safety and clinical significance of total thyroidectomy performed for benign thyroid disease. Methods Eighty-eight patients with benign thyroid disease were treated with total thyroidectomy. The postoperative complications were analyzed. Results With primary total thyroidectomy, the incidences of transient hypocalcemia and transient recurrent laryngeal nerve paralysis were 2.5% and 1.2% respectively. The incidences after reoperation were 28.6%( P

0. 05). The mean survival time in Iodine-125 stent group was 10. 5 months prominently longer than those in group A 4. 4 months (P

One hundred kinds of excellent books on basic medicine were selected from Wanfang Data Knowledge Platform to develop a books-papers citation database.Their academic levels of the selected books were evaluated by PageRank algorithm.The chapters and sections of the books were divided into 4 parts according to their PageRank value and the distribution characteristics of their citation frequencies to study the PageRank and the top 20 cited chapters and sections of the books.The advantages and disadvantages of PageRank algorithm in book evaluation were described.

Objective To investigate the protective effect of Limb ischemic postconditioning ( LIPostC) on blood brain barrier (BBB) and explore the influence on the the expression of tight junction protein claudin-5,occludin in cerebral ischemia-reperfusion injury of mice.Methods Ninety male CD1 mice were divided into three groups randomly:Sham-operated group(sham group), ischemia-reperfusion group(I/R group) and LIPostC group.Mouse middle cerebral artery cerebral ischemia-reperfusion model was established by modified-occlusion method. Neurological deficit scores, brain water content, infarct volume, BBB dysfunction were measured at 24 h after reperfusion.Western blot and RT-qPCR were used to analyze the expressions of claudin-5, occludin.Results Compared with I/R group, neurological deficits scores were significantly decreased, brain edema was relieved, infarct volume was reduced, and the expression of claudin-5, occludin up-regulated, the differences were statistically significant(all P<0.05).Conclusion LIPostC can protect the BBB of cerebral ischemia-reperfusion injury mice by increasing the expression of claudin-5,occludin, and that will provide a theoretical basis for its clinical application.