Objective To establish the quality standard for Kangganmao Granule.Methods Radix scutellariae,radix et rhizome glycyrrhizae and radix platycodonis in the prescription were identified by TLC.The content of the baicalin was determined by HPLC.The column of AichromBond-AQ-C18(4.6 mm? 150 mm,5 ?m)was used.The mobile phase was CH3OH-H2O-H3PO4(43:57:0.2),with the flow of 1.0 mL/min,the column temperature of 30 ℃,and peaks were detected at 280 nm.Results The characteristic of identification by TLC was distinct and highly specific.The linear range of baicalin was 0.049 6～0.297 6 ?g,r =0.999 7,the average recovery was 101.80%,RSD=1.86%(n=6).Conclusion The method was easy to operate with accurate result and good reproducibility.It can be used effectively for the quality control of the preparation.

Objective To evaluate left ventricular torsion in patients after coronary artery bypass graft(CABG) surgery by velocity vector imaging(VVI). Methods Twelve patients who were scheduled to have CABG surgery were enrolled. VVI offline software was used to evaluate myocardial rotation in both basal and apical segment before and three months after CABG. The rotation and rotation rate of each a counterclockwise rotation at the apex and a clockwise rotation at the base, and the absolute value of CABG the rotation at the base was significantly improved than that before CABG (P <0.001), and the rotation in the segment of anterior septum, anterior wail and inferior septum at the base was higher than that rotation in the apical and basal segment three months after CABG was also significantly higher than that negative peak rotation rate in the early diastolic period at the apex were significantly higher than those at the improved than that before CABG (P <0.001). And the positive peak rotation of the systolic period in the segment of anterior septum,anterior wall,lateral wall and inferior septum and the negative peak rotation of the diastolic period in the segment of anterior and inferior septum at the base were also higher than those before CABG (P <0.001). Conclusions VVI is a useful method which can quantitatively assess left ventrieular torsion in patients before and after CABG surgery.

Objective To assess whether echocardiographic parameters of M-mode,pulsed-wave Doppler and tissue Doppler imaging which were usually used for selecting cardiac resynchronization therapy (CRT) candidates could predict CRT responders.Methods Twenty-nine patients with NYHA class Ⅲ or Ⅳ heart failure,left ventricular ejection fraction(LVEF)≤35%,QRS≥130 ms were enrolled.All of them underwent biventricular pacemaker implantation.The septal-to-posterior wall motion delay(SPWMD) from M-mode recording,and the standard deviation of time to peak systolic velocity(Ts-SD) were used to evaluate intraventricular dyssynchrony.The interventricular mechanical delay (IVMD) by pulsed Doppler echocardiography was calculated by subtracting the right ventricular preejection intervals (RV-PEI:the onset of the QRS on the electrocardiogram to the onset of pulmonary outflow) from the left ventricular preejection intervals (LV-PEI:the onset of the QRS on the electrocardiogram to the onset of aortic outflow).At least 15 % reduction in left ventricular end-systolic volume(LVESV) at the 6-month after CRT was defined as responder.Results In the 29 patients,there were 20 (68.97%) responders.The area under the receiver operating curve(ROC) of SPWMD,Ts-SD,LV-PEI,RV-PEI and IVMD to predict CRT responders was 0.448 (95% CI 0.184-0.712,P＞0.05),0.557 (95% CI 0.317-0.798,P＞0.05),0.479 (95% CI 0.186-0.773,P＞0.05),0.568 (95 % CI 0.272-0.863,P＞0.05) and 0.411 (95% CI0.136-0.687,P＞0.05),respectively.Conclusions M-mode echocardiography,pulsed Doppler and tissue Doppler imaging failed to predict CRT responders.New echocardiographic techniques including threedimensional echocardiography and speckle tracking image will be investigated,and may improve the predictive power.

Objective To optimize the prescription and technology of Brucea javanica oil subnanoemulsion injection. Methods Using superfine Brucea javanica oil as oil phase,yolk lecithin and poloxamer 188 as the complex emulsifying agents. The dosage of oil,emulsifier and stabilizer were optimized and the preparation was optimized by orthogonal experiments. Results The optimum precription was 10% oil phase,1.2% emulsifier and 0.1% stabilizer. The optimum technology was that aqueous phase and oil phase mixed at 70 ℃,sheared 20 min by high speed shearing machine and then passed the ultra-high pressure nano homogenizer 6 times under the pressure of 900 bar. Conclusion The physical stability of Brucea javanica oil subnanoemulsion injection is good.

Objective To evaluate the effect of transarterial chemoembolization (TACE) before operation, including side effects and the improvment for the suceesfult resection rate.Methods 56 patients with cardiac cancer were divided into two groups: TACE group and resection group. The former included 25 patients (male 14, female 11) undertaken TACE 7-15 days before surgery. The image manifestations, histological changes and side effects together with the time of operation, volume of blood lose during operation and the succesful resection rate were compared with those of the latter. Results 1. TACE induced the necrosis of tumor tissue to some extent and possessed the power to localize the lesion with little side effects. 2. TACE before resection did not only minimize the injury of operation but also improved the resection rate. Conclusion TACE is a safe, effective modality before operation and is worthy for recommendation

Objective To analyze the efficacy and experience of coil embolization in patients with pulmonary arteriovenous malformations(PAMV). Methods Seven patients (2 males and 5 females, age range from 11 to 52 years; mean age 31 years) were admitted including 4 single saccular, 2 multiple saccular, 1 diffuse capillary pulmonary arteriovenous malformations. Results Coil embolization of pulmonary arteries was successful in all 7 patients. The mean arterial oxygen saturation rate increased from 87% to 96% in patients with saccular PAMV. Transient chest pain occurred in a case of diffuse capillary PAMV during the procedure, after undergoing multiple coil embolization, the arterial oxygen saturation rate increased from 79% to 87%. All patients were followed up for 6 months to 5 years, arterial oxygen saturation declined slightly,and mild to moderate tricuspid regurgitation was found by echocardiography only in the patient with diffuse capillary PAMV.Conclusion Coil embolization is an effective and safe method of treatment for saccular PAMV. But it is only a palliative therapy for diffuse capillary PAMV.

Objective To compare the safety and recent efficacy between transcatheter and surgical closure of secondum atrial septal defect over 50 years of age. Methods Retrospective analysis was done on 66 patients over 50 years of age who underwent transcatheter (30 cases) and surgical (36 cases) closure of secondum atrial septal defect.The median age was 60.8?7.1 years old in the transcatheter closure group and (57.5?6.3) years old in the surgical closure group( P=0.04).The median sizes of atrial septal defect in the transcatheter and the surgical closure group were 24.9?7.2 mm and 30.4?11.0 mm respectively (P=0.02). We compared the recent efficacy、 complications and prognosis between two groups. Results The instant procedural success rate was 93.3% for the transcatheter closure group and 100% for the surgical group (P=0.85). 2 procedures in the transcatheter closure group failed, 1 patient (2.6%) in the surgical group died during hospitalization and 9 patients (25.0%) underwent Devage tricuspid annuloplasty. Newly onset atrial arrhythmias occurred in 3.3% in the transcatheter closure group and 22.2% in the surgical closure group (P=0.03). After the procedures, right atrial and ventricular dimensions were decreased from 38.0?9.0 mm to 35.2?10.1 mm and from 44.2?8.6 mm to 36.2?9.0 mm in the transcatheter closure group whereas in the surgial group, the atrial and ventricular sizes fell from 44.1?8.2 mm to 38.2?9.5 mm and 49.0?10.2 mm to 37.1?11.0 mm respectively (P

In order to study the application of glial cell line-derived neurotrophic factor(GDNF)in clinic,gene mutation,fusion protein expression in E.coli and purification methods have been used to obtain the fragments of GDNF,GDNF(△N39),GDNF(△N39)-R9.Using primary cultured dopaminergic neurons and PC12 cells with transfected with GFR?1 and Ret to observe their biological function and cytotoxicity.Using B-Endo3 cells and Transwell method to analyze their delivery across the cellular membrane and blood brain barrier.The results show that GDNF(△N39)-R9 has the same neurotrophic function with wild GDNF and nearly no cytotoxicity to dopaminergic neurons and PC12-GFR?1-Ret cells and can get through effectually the cellular membrane and simulacrum of blood brain barrier with matrigel and B-Endo3.

Objective To investigate the linkage between the mutation of IL 4R ? chain gene and susceptibility of asthma and atopy in Wuhan. Methods Thirty five samples from asthmatic children,twenty five samples from adult patients with asthma, twenty samples from health adult and children respectively, were analyzed. A coding region 1205 1928 of IL 4R ? chain gene was detected by reverse transcription polymerase chain reaction/Single Strand conformation polymorphism/Sequence. Result 57.8% cases from asthmatic children, one polymorphism (1902 A→G), were found exist in the IL 4R ? chain gene and only 8% cases from asthmatic adults and 5% cases from control have this mutation. Conclusion The SNP of IL 4R ? chain coding area 1902 correlates with susceptibility of asthma in Wuhan asthmatic children (? 2=12.54, P 0.05, ?=0.05). There may be another mutations in IL 4R ? chain coding area except 1902 or another changes in other gene related with allergy.

Objetive To analyze the standard of training and evaluation on semi-automatic biochemical analyzer to improve grass-root medical unit in training on the analyzer.Methods The standard was analyzed and expounded from the aspects of scope of application,requirements,subjects setting,key links as well as the demands of evaluation scoring scale.Results The main points of the standard included elementary knowledge of biochemical analysis as well as the structure,principle,installation,operation,application,maintenance and etc of the semi-automatic biochemical analyzer.The integration of personnel and instrument contributed to accurate and rapid output of test report.Conclusion It is necessary to grasp accurately the connotation of the standard to improve the quality of medical equipment training.