Objective To evaluate the bridging role of non-bioartificial liver for patients with chronic severe hepatitis B awaiting liver transplantation. Methods The non-bioartificial liver support was used to treat 9 hepatitis B patients with advanced stage liver failure waiting for liver donor in order to correct physiological disturbances and bridge to orthotopic liver transplantation (OLT). Results When the patients were treated with non-bioartificial liver support, the concentrations of serum total bilirubin and total bile acid were decreased dramatically (P

With the common application of total mesorectal excision (TME) technique,the proportion of sphincterpreserving surgery for the mid-low rectal carcinoma is significantly increased.Anastomotic leakage after sphincter-preserving surgery is the most severe complication of rectal surgery,and it is the main reason which will lead to other complications and death.Many researches on the early diagnosis,prevention and treatment of anastomotic leakage are conducted by surgeons at home and abroad,and a further understanding of this complication is deeply realized.

For observation on clinical therapeutic effect of modified Wei Er Fang on gastric precanceraus lesion (GPL), 90 cases of GPL were divided into 3 groups, the treatment group treated with modified Wei Er Fang, the control group I treated with Wei Fu Chuan and the control group I with Bismuth subcitrate, 30 cases each group. Results indicated that the total effective rate for clinical symptoms was 96. 67% in the treatment group which was significant different from 60. 00% in the control group I (P 0. 05), but the therapeutic effect on heterotypic proliferation in the treatment group was superior to those in the two control groups. It is showed that modified Wei Er Fang has a better action of reversing gastric precancerous lesion.

Objective To study the hypnotic interaction between propofol and ketamine with isobologram. Methods Seventy-five ASA Ⅰ - Ⅱ patients (35 male, 40 female) aged 20-50 yr, weighing 40-80 kg, undergoing elective upper abdominal surgery were randomly divided into three equal groups of 25 patients : propofol group (P); ketamine group (K) and propofol-ketamine combination group (P/K), Each group was further divided into 5 subgroups. Propofol 0.8, 1.0, 1.25, 1.56 or 1.95 mg ? kg-1 was given in 5 propofol subgroups (P1-5 ) respectively. Ketamine 0.32, 0.40, 0.50, 0.63 or 0.78 mg?kg-1 was given in 5 ketamine subgroups (K1-5 ) respectively. Propofol /ketamine 0.45/0.15, 0.60/0.20, 0.80/0.29, 1.05/0.35 or 1.41/0.47 mg?kg-1 were given in the 5 propofol-ketamine combination subgroups (P/K1-5 ) respectively. Two minutes after drug administration the patients were asked to open their eyes. Failure to open eyes was taken as the start-point of hypnotic effect. If the patient failed to respond to verbal order twice consecutively, the patient was considered to be in the hypnotic state. When the patient in hypnotic state failed to respond to electric stimulation of certain intensity, the patient was considered to be in anesthetic state. ED50s of propofol, ketamine and P/K combination for hypnotic and anesthetic effect were calculated. Isobologram was drawn. ED50 and 95 % confidence limit of ketamine were plotted on the abscissa and of propofol on the ordinate. The ED50 s of the two drugs were connected. If the ED50 of P/K combination was located on the connecting line, the two drugs are additive, on the left side of the connecting line synergistic, on the right side of the connecting line antagonistic. SBP, DBP, HR, SpO2 and BIS were continuously monitored before, during and after drug administration. Results The ED50 s for hypnotic and anesthetic effect were : 1.15 mg? kg-1 and 1.59 mg? kg-1 in group P; 0.40 mg? kg-1 and 0.72 mg? kg-1 in ketamine group; 0.65/0.22 mg?kg-1 and 1.19/0.40 mg?kg-1 in P/K group. The deviation of the location of ED50 of P/K combination from the connecting line was statistically insignificant. There was no significant change in SBP and DBF after administration of drugs in P/K group. Conclusion The hypnotic and anesthetic interaction between propofol and ketamine was additive. In terms of hemodynamic stability, P/K combination was the best among the three groups.

c-Jun N-terminal kinases(JNKs)play an integral role in neuronal death in multiple cell lines following a wide variety of stimuli and in a number of physiological functions which have been recognized as important enzymes in cellular function.JNK has been implicated in several neurodegenerative diseases.Data are emerging to extend the understanding of the JNK signaling and confirm the possibility that targeting JNK signaling may offer an effective therapy for pathological conditions in the near future.Because of the involvement of JNK in neuronal diseases,the inhibition of this enzyme is an attractive therapeutic target.

Background Corneal transplantation is an effective treatment to severe corneal diseases,but the shortage of cornea donor limits its application.Tissue-engineered cornea is being a new approach to corneal diseases.Objective This study was to investigate the possibility of construction of tissue-engineered corneal epithelium by culturing human amniotic mesenchymal stem cells (hAMSCs) in vitro.Methods Fresh human amniotic membranes were obtained under the approval of Ethic Committee of Affiliated First Hospital of Jinan University and informed consent of maternal women.The 6 cm×6 cm amniotic membrane tissue explant was digested using trypsin+ EDTA,and then the amniotic epithelial cells (AECs) were scraped before putting into collagenase Ⅱ digestion medium to isolate hAMSCs.hAMSCs of passage 3 were cultured to achive 80％-90％ confluence,and then the ceils were incubated on rabbit deepithelial corneal stroma at a 1 ×105/ml density.The corneal stroma was co-cuhured with hAMSCs at an air-liquid interface till 14 days.Rabbit deepithelial corneal stroma with and without hAMSCs (experimental group and control group) were fixed in 4％ para formaldehyde, and sections were prepared for histopathological examination.Immunochemistry and immunofluorescence were empoyed to detect the expressions of cytokeratin3 (CK3) and CK12 in hAMSCs.Results hAMSCs grew well and formed a stratified epidermal structure resembling native corneal epithelium on rabbit corneal stroma in cultured 14 days in the experimental group,with the oval nucleus at basement and fusiform nucleus on the surface of corneal stroma.There was no cell structure in the control group.Immunochemistry revealed brown staining for CK3, CK12 in cytoplasm of hAMSCs on the rabbits corneal stroma,and the green fluorescence for CK3 and CK12 was also seen in the hAMSCs.However,the response for CK3 and CK12 was absent in the control sections either immunochemistry or immunofluorescence test.Conclusions hAMSCs can be induced to differentiate into corneal epithelioid cells at an air-liquid interface on the rabbit corneal stroma.

Objective To study of manifestation of adenomyomatosis of gallbladder from MRI and to improve its accuracy of diagnosis .Methods MR features of 24 cases with adenomyomatosis of gallblad-der from 2006 to 2008 proved by operation and pathology were analysed retrospectively .Results Among 24 patients, 20 cases were diagnosed correctly before operation ,four were misdiagnosed as Chronic cholecysti-tis and carcinoma of gallbladder respectively; all cases of gallbladder wall increased thickness from 5 to 12mm (7mm average).Among them, the gallbladder -wall was thickened as limited type in fourteen cases , segment type in eight cases and diffuse type in two cases .And classical Rokitansky-Aschoff sinuses were clearly displayed in 20 cases by lamina T2WI from MRI .Conclusion MRI might has higher diagnostic value in diagnosing of adenomyomatosis of gallbladder .

Objective To evaluate the current status and outcomes of pelvic exenteration (PE) for recurrent cervical cancer.Methods The following electronic databases has been searched on recurrent cervical cancer management and treatment:Chinese Biological Medicine Disk (CBM),PubMed and Cochrane library.All retrieved studies had to fulfill the following inclusion criteria:cohort studies of recurrent cervical cancer,containing information of detailed patient and operation characteristics as well as the survival rate.Only publications in the English literature were included.All eligible literatures between Jan.1990 and Aug.2013 were assessed for quality.Relevant basic characteristics,complications,survival rate and prognostic factors were reviewed.Results There were eight trials involving 607 patients with cervical cancer received PE,including 515 cases with recurrent disease and 92 cases with primary disease.Four hundred and ninety patients had received total pelvic exenteration (TPE) operation,103 underwent anterior pelvic exenteration(APE) and 14 received posterior pelvic exenteration(PPE).The 5-year overall survival rate for recurrent cervical cancer fluctuate from 26.7％ to 56.0％.Complication rates were from 34.3％ to 83.3％ and the mortality rate was 1.2％ (7/607).Among the relevant factors affecting survival time,resection margin status seemed to be the most important.Conclusion Based on this systematic review,PE does help improve the survival of recurrent cervical cancer patients on the basis of strict selection of candidates.

ObjectiveTo discuss the features and laws of online learning of graduate studnets in Beijing University of Chinese Medicine (BUCM); To provide references for the directions of guidance and improvement. Methods Graduate students from the grade 2014 in BUCM were set as the investigation objects. Questionnaire was used to investigate students’ online learning experience, learning channels, time frequency, main contents for online learning, learning achievements and shortcomings, and cognitive degree of MOOCs. Students were invited to write down their understanding of online learning and their opinions and suggestions for the new learning mode of combining MOOCs and classroom learning.Results In this survey, 92.16% of graduate students had the experience in online learning; 41.18% of the students did online learning through the campus network; 47.06% of students kept“once a week” frequency of online learning; 54.90% of students learnt through the network mainly used to develop extracurricular knowledge; 66.27% of students thought online learning had a certain learning harvest; 49.41% of students thought complicated network information brought certain problems. In the MOOCs cognitive survey, 43.92% of students did not understand MOOCs completely; 66.27% of students wanted to try MOOCs as a new study mode; 30.98% of students thought the hours of MOOCs should accounts for a quarter of the total class hours.Conclusion Most graduate students have experience in online learning, have certain learning achievements and have expectations for MOOCs teaching.

Several factors influencing the extraction of tetrandrine and demethyltetrandrine from Chinese medicinal herbs, Stephania tetrandra S. Moore were studied by means of Ortogonal method. The optimum Condition was eight hours steeping, in 33.3% ammonia in absolute alcohol for four hours under ultrasonic agitation. Tctrandrine aud demethyltetrandrino were detected by HPLC. The contents were 0.678% and 0.616%, the detection limits were 3.87?10-3and 0.48?10-3 mmol/L respectively.