Currently targeted therapy of breast cancer therapy has become the hot research field and a kind of brand-new biological treatment mode after three traditional pattern of the surgery, radiotherapy and chemotherapy for breast cancer. Molecular targeted therapy is aimed at the target that may cause cancer cells, such as protocarcinogenic genes and tumor-suppressor genes, cell signaling pathways, cytokines and receptors, antiangiogenesis etc. It can reverse malignant biology behavior to inhibit tumor cell growth from the molecular level and has the advantages of high specific effects and low side effects. This paper focuses on the drug of the molecular targeted therapy for breast cancer and the latest progress of targeted therapy.

OBJECTIVE: To establish a bacterial endotoxin test method for fluorescein sodium injection.METHODS: Based on the bacterial endotoxin test method stated in Chinese Pharmacopoeia 2005 edition(second part),an interference test was conduced on different batches of fluorescein sodium samples using tachypleus amebocyte lysate from different companies.RESULTS: At a concentration of no more than 0.75 mg?mL-1,fluorescein sodium showed no interfere with the bacterial endotoxin test.CONCLUSION: It is feasible for bacterial endotoxin test of fluorescein sodium injection be conducted in a limit value of 0.15 EU?mL-1.

Ischemic stroke is one of the major diseases of causing adult disability and death.Age,sex,smoking,hypertension,and diabetes,etc.are the risk factors for ischemic stroke,but they can only partially explain the reasons of stroke onset.Twins,families,and single-gene genetic stroke studies have shown that ischemic stroke has obvious genetic predisposition.In recent years,genomics has made remarkable progress in ischemic stroke study and has shown that many new single nucleotide polymorphisms or genes are associated with ischemic stroke and its risk factors.However,the contribution of these genetic genes for ischemic stroke is still too small and the repetitive studies are needed for further confirmation.

Objective To establish the quality standard for Kangyuan Granula. Methods Radix Astragali, Radix Paeoniae Alba, Fructus Lycii and Radix et Rhizoma Glycyrrhizae in this prescription were identified by TLC. The content of the Paeoniflorin was determined by HPLC. The column of AichromBond-AQ-C18 (4.6 mm?150 mm, 5 ?m) was used, the mobile phase was Water-Acetonitrile (85∶15), at the flow of 1.0 mL/min, the column temperature of 30 ℃, and peaks were detected at 230 nm. Results The characteristic of identification by TLC was distinct and highly specific. The linear range of Paeoniflorin was 0.0545～0.545 ?g, r =0.999 5, the average recovery was 97.90%, RSD=1.06% (n =6). Conclusion The method was easy to operate with accurate result and good reproducibility. It can be used effectively for the quality control of this preparation.

Sepsis is a systemic inflammatory reaction caused by infection,which is a common complication after bum,serious trauma,major surgery,pathological obstetrics,toxication and cardiopulmonary resuscitation.Simultaneously,it's also an important cause which can lead to septic shock and multiple organ dysfunction syndrome.Heart is the target of multiple organ dysfunction caused by sepsis,leading to different degrees of cardiac dysfunction by sepsis and vice verse.Ryanodine receptor is macromolecule composed by complex protein structure,which is the major component of Ca2+ channels.It's the necessary factor to adjust muscle,cardiac rhythm and contraction.It is also involved in regulating sarcoplasmic reticulum to release Ca2+.This article is to give a review of ryanodine receptors in cardiac dysfunction caused by sepsis.

Two groups of neurotic outpatients were compared in their outcome afterfrontal EMG biofeedback treatment with Jacobson progressive relaxation at home.The firstneurotic group consisted of 42 patients and treated once every two days for four weeks whilethe second group consisted of 19 cases and treated every day but for only two weeks.Similarily satisfactory clinical effects and same trends of change in various physiological indexof EMG and clinical symptons were observed,between these two groups.No relationship wasfound between patient's personality characteristc and clinical effects.In a four follow up,2/3patients reported a continuous satisfactory outcome.

Objective To understand the role of peroxisomes on the intracellular survival of Salmonella typhimurium in macrophages. Methods Vesicles from infected or uninfected RAW264.7 macrophage-like cells were isolated by stepwise centrifugation after cells were broken by nitrogen cavitation and purified with magnetic beads containing polyclonal antibodies to TassC (Target for Salmonella secreted protein SpiC). Western-blot was used to detect the characters of the binding vesicles. Three-dimensional (xyz) fluorescence microscopy was used to determine the recruitment of peroxisomes tagged with pDsRed2-Perxi to the SCV after infection of RAW264.7 cells with Salmonella typhimurium mutant strains spiC:kan producing GFP. Volocity software was employed for image analysis. Overlap of individual fluorescence pixels from separated channels for each optical plane was determined with the Volocity 4.1 colocalization module. Results It was shown by western-blot that the anti-TassC magnetic beads could bind TassC and peroxisomal marker catalase in infected or uninfected RAW264.7 cells. Inducible nitric oxide synthase (iNOS) could be detected in infected samples, but couldn’t in uninfected samples. Lysosome associated membrane protein (LAMP1), one of the lysosome markers, and a bacterium marker RecA could not be detected from the elution of anti-TassC magnetic beads. It was determined by a fluorescence microscopy that the recruitment or overlapping of peroxisomes tagged with pDsRed2-Perxi to the Salmonella-containing vacuoles after infection of RAW264.7 cells with Salmonella mutant strains producing GFP. Calculation by Volocity 4.1 showed that peroxisome abundance increased by 1.2- or 1.3-fold, respectively, at 1h and 24h time point of infection in the infected macrophages than in uninfected cells while the bacteria abundance decreased with the infection time. Conclusion It is suggested that TassC is localized with peroxisomes, but not with lysosomes. Inducible nitric oxide synthase might be localized to peroxisomes and recruited to the SCV during infection with Salmonella mutant strains for killing the bacterium.

OBJECTIVE: To analyse oily constituents of Meconopsis punicea Maxim. METHOD: The oily constituents were analysed and identified by GC-MS. RESULTS: The sixty-three constituents were isolated and the forty-seven of that were identified, making up 74.6% of the total constituents. Ester compounds accounted for 57.5% of the identified compounds. The main constituents were ester octadecnate. CONCLUSION: Ester octadecenate has high biological activity, which agrees with curative effect of the plant.

The parameters of 797 measurements of electrolytes and blood gases in 191 patients during and after open heart surgery under cardio-pulmonary bypass were studied. It was found that both the anion gap (AG) and lactate levels were markedly elevated in the patients than in the normal controls. It is well known that increased plasma lactate due to hvpoxia is one of the main factors to result in anion gap acidosis, and the therapeutic measures should be directed to increase tissue oxygen supply rather than administer alkali, which will be liable to induce alkalosis. Therefore, it is suggested that concomitant determinations of AG and blood gases bo valuable and helpful to the management of the postoperative cases of open heart surgery.