Objective To investigate the clinical effects of Xuebijing injection on immune function of patients with acute exacer-bation of chronic obstructive pulmonary disease(AECOPD) .Methods 103 patients with AECOPD were divided into 3 groups ,in-cluding control group(33 cases) ,the treatment group 1(36 cases) and treatment group 2(34 cases) .The conventional treatments of COPD including anti-infection ,relieving cough ,expectorant ,antispasmodic ,anti-asthmatic and aerosol therapy were adopted in all patients ,based on the similar conventional therapy in the control group ,50 mL Xuebijing injection with NS 250 mL(1/d ,course of 7 d) were added in the treatment group 1 ,and 100 mL Xuebijing injection with NS 250 mL (1/d ,course of 7 d)were added in the treatment group 2 .Serum CD3 ,CD4 ,CD8 ,CD4/CD8 and IgA ,IgG ,IgM were tested before treatment as well as 4 days and 8 days after treatment .The condition changes in all patients were evaluated .Results After 8 days of treatment ,the total treatment effi-ciency and serum CD3 ,CD4 ,CD4/CD8 ,IgA were significantly changed in the two treatment groups compared with the control group(P<0 .05) ,but serum CD8 ,IgG ,IgM showed no significant difference(P>0 .05);After 4 days of treatment ,the total efficien-cy and serum CD4 ,CD4/CD8 were significantly changed in the treatment group 2 which injection dose was increased compared with the control group(P<0 .05) .The total treatment efficiency and immune function showed no significant difference between the two treatment groups(P>0 .05) .Conclusion Xuebijing injection can improve the immune function and treatment efficiency of AECO-PD patients .Appropriately increase the dose can achieve better clinical results in a short period .In conclusion ,Xuebijing injection is a feasible and effective method for the clinical treatment of AECOPD .

Objective To evaluate the effect of a community-based nutrition education program on the dietary quality of the elderly with diet balance index(DBI). Methods Eighty-three volunteers from Zhujing Town of Jinshan District of Shanghai with age ≥60 years were served as study objectives,and a total of 70 people completed the program.They were given nutritional guidance bimonthly in combination with measures such as community publications,panel discussions and individual interviews.Measurements of dietary intakes,which lasted for 3 d, were performed before intervention and 12 months after intervention,respectively.Diet quality was evaluated with DBI scoring system,and the parameters were compared before and after intervention. Results There were significant differences in DBI-Total Score(DBI-TS),DBI-Lower Bound Score(DBI-LBS)and DBI-Diet Quality Distance(DBI-DQD)before and after intervention for these 70 people (P<0.05).The proportion of subjects with adequate milk and soy product consumption increased from 7.1% before intervention to 20.0% after intervention,that with proper amount of salt intake from 22.9%to 51.5%.and that with diverse diet from 20.0%to 49.9%.The major DBI pattern changed from pattern B (47.1%) and pattern E(34.3%)to pattern B (34.3%) and pattern A (32.9%). Conclusion The community-based nutrition education program for the elderly can improve the diet quality of the elderly.

Objective To analyze the differential expression of protein in taxol-resistance from elderly females with ovarian cancer, by compared proteomics analysis and identified the candidated marker in the clinical tissues. Methods Soluble fraction proteins in two SKOV3 taxol-resistant cells, SK-TR30 and SK-TR2500 and one A2780 taxol-resistant cell, A2780-TR paired taxol-sensitive ovarian cancer cells were separated and prepared by two dimensional gel electrophoresis (2-DE). The differentially expressed proteins were selected and identified by matrix-assisted laser desorption/lonization time of flight mass spectrometry(MALDI-TOF-MS) and database search. 2-DE profiles with high resolution and reproducibility were obtained. Then highly expressed protein-cofilin1 which exist in two forms of de-phosphorylate was detected and compared between human ovarian cancer specimens including 22 elderly female with chemosensitive and 21 elderly female with chemoresistant by immunohistochemistry. Results Among the identified proteins, there were sixteen over/under-expressed proteins in taxol-resistant cell lines as compared with taxol-sensitive cell lines. In particular, overexpression of cofilin1 and destrin were displayed in all three taxol-resistant cell lines. The expression of phosphorylation of cofilin (P＜0.05), but not cofilin1, displayed much higher in chemoresistant than in chemosensitive group. Conclusions Cofilin1 may play a role of taxol resistance by phosphorylation of ovarian cancer cell. However, the mechanisms need to be elucidated further.

Objective To investigate the impacts of psychosocial factors on the development of breast cancer.Methods Thirty-seven patients with confirmed breast cancer and 37 healthy women were enrolled in this study to complete psychological assessment,including ENRICH marital inventory,Life Event Scale(LES),Social Support Rate Scale(SSRS),and Trait Copying Styles Questionnaire(TCSQ).Results In comparison with the healthy controls,the patients experienced more stress events and negative response,and lowered quality of marriage over the last 5 years.The logistic regressive analyses indicated that the important contributors to breast cancer were stress events,objective social support and negative coping styles.Conclusion Stress events,insufficient access to available psychosocial support and negative responses are important risk factors of breast cancer in women.

Objective To explore the better diagnostic method for acute splenic arteral injury through comparative analysis of CT and DSA. Methods Fifty-seven patients with acute splenic injury were examined by CT and DSA,treated with splenic arterial embolization and then undertook follow up. Results CT examination possessed higher sensitivity and accuracy than DSA in demonstrating splenic parenchymal laceration,intrasplenic hematoma,subcapsuler hematoma,rupture of splenic capsule and combinated injury of intra-abdominal organs,especially in localizing splenic laceration.And there was a high significant difference statistically between the two kinds of examination(?~2=10.71,P

ObjectiveTo explore the best applying time and effect of baclofen on recovery of motor function of stroke patients with hemiplegia.Methods83 stroke patients with hemiplegia were divided into group A and group B according to the degree of muscle tension, every group was divided into the treatment group and control group. Patients of the four groups were treated with routine rehabilitation training, and baclofen was added to the patients of two treatment groups. Motor ability and activities of daily living (ADL) of patients were evaluated before and after one month treatment.ResultsAfter one month treatment, motor ability and ADL of all patients were improved, but in group A, there were not significant differences existed between treatment group and control group (P>0.05), while, there was a significant difference existed in group B(P<0.01).ConclusionBaclofen is necessary to stroke patients with hemiplegia only when the muscle tension increased obviously (≥Asworth 3).

BACKGROUND:So far, engineered myocardium is stil facing many problems. Research has demonstrated that bone marrow mesenchymal stem cells can be differentiated into myocardial cells. Polyglycolide and polycaprolactone are commonly used artificial polymers, which have good biocompatibility.
OBJECTIVE:To observe the growth of the poly(glycolic acid)/poly(e-caprolactone) copolymer patch in vitro in normal myocardium and infarcted myocardium.
METHODS:Bone marrow mesenchymal stem cells in Sprague-Dawley rats were separated using adherent separation and selection method, cultured in vitro. The third passage was labeled with DAPI. The bone marrow mesenchymal stem cellsuspensions (2×106/cm2) were produced and planted on poly(glycolic acid)/poly(e-caprolactone) copolymer scaffolds to form poly(glycolic acid)/poly(e-caprolactone) copolymer patch. After culturing for 48 hours, the specimens were observed under electron microscope, stained with hematoxylin and eosin, and then observed under light microscope. Rat models of myocardial infarction were established by ligating left anterior descending coronary artery. Poly(glycolic acid)/poly(e-caprolactone) copolymer patch was implanted into the normal and infarcted myocardium for 5 weeks. The survival of bone marrow mesenchymal stem cells was determined by the detection of pathology.
RESULTS AND CONCLUSION:Results of light microscope and electron microscope demonstrated that bone marrow mesenchymal stem cells grew three-dimensional y on poly(glycolic acid)/poly(e-caprolactone) copolymer patch. cells and patch were adhesive wel . Under laser confocal microscopy, compared with the first week, bone marrow mesenchymal stem cells were marked by DAPI in the myocardium at the fifth week. There were bone marrow mesenchymal stem cells marked by DAPI in the infracted area. Results of hematoxylin-eosin staining exhibited that bone marrow mesenchymal stem cells were detected in the infarct area. These results suggested that bone marrow mesenchymal stem cells adhered to the poly(glycolic acid)/poly(e-caprolactone) copolymer stent wel . The complexes of poly(glycolic acid)/poly(e-caprolactone) copolymer and bone marrow mesenchymal stem cells can be used for reparation of myocardium.

Objective To determine the diagnostic value of diffusion weighted imaging(DWI) for primary nasopharyngeal carcinoma(NPC) and metastatic lymph nodes,and to establish the diagnostic thresh-old of apparent diffusion coefficients(ADCs). Methods Conventional MR scans and DWI scans were con-tinuously performed in 56 patients with newly diagnosed NPC and 55 healthy volunteers. All patients re-ceived primary tumor biopsy and MR image-guided cervical lymph node fine-needle biopsy. ADC and eADC values of both primary lesions and lymph nodes were calculated and compared. Results According to the pathological diagnosis,all the 56 patients had non-keratinizing carcinoma and 51 had lymph node metastasis. In the control group,75 cervical lymph nodes were found. ADC values of both primary NPC and metastatic lymph nodes were significantly lower, while eADC values were higher than those of normal controls. Setting the ADC value threshold at 0.809 ×10-3 mm2/s, the sensitivity and specificity for primary NPC detection were 80.4% and 74.5%, respectively. The negative and positive predictive values were 79.2% and 77.6% ,respectively. The accuracy was 78.4%. Setting the ADC value threshold at 0. 708×10-3 mm2/s, the sensitivity and specificity in the detection of metastatic cervical lymph nodes were 43.1% and 93.3%, respectively. The negative and positive predictive values were 70.7% and 81.5% ,respectively. The accura-cy was 73.0%. Conclusions DWI might be a new diagnostic approach in the detection of primary NPC as well as metastatic lymph nodes.

The paper described the specific measures and results of postoperative complications reporting system, including reporting coverage, methods, deadline, data management, supervision and feedback. In addition, it introduced results of the practice and the positive impact on quality of care and safety management. Based on these, the authors proposed further improvements of the system for the purposes of constant improvement of care and higher medical safety.

Objective To identify the potential value of event-related potentials ( ERPs ) and electroencephalogram(EEG) in early diagnosis of dementia.Methods From June 2008 to July 2011,113 patients with dementia were recruited fom First Affiliated Hospital of Anhui College of Traditional Chinese Medicine.Meanwhile,100 healthy individuals conducting physical examination at this hospital were collected as the control group.P300 and EEG were measured in patients and healthy controls.Results ( 1 ) Compared with healthy controls,patients with early diagnosis of dementia had significantly longer latency(402.5 ± 37.4)ms vs.( 320.4 ± 23.5 ) ms ;t =19.40 ; P =0.02 ) and lower amplitude of P300 ( 3.76 ± 1.76 ) μV vs.( 5.32 ± 1.38 )μV;t=7.24;P=0.01 ).(2)Significant higher proportionof abnormal EEG cases was found in individuals with dementia (68/113,60.2％ ) than that of participants in the healthy control group(34/100,34.0％ ) ( x2 =13.54,P ＜0.01 ).Specifically,patients with early diagnosis of dementia had significantly higher prevalence of moderate (26.5 ％ [ 18/68 ] vs.5.9 ％ [ 2/34 ] ; x2 =4.85,P =0.02 ) and severe ( 8.8 ％ [ 6/68 ] vs.2.9 ％ [ 1/34 ] ; x2 =1.22,P =0.02 ) abnormal EEG than healthy controls,while there were no significant difference of the proportions of mild or borderline abnormal EEG between the two groups ( P ＞ 0.05 ).Conclusion P300 is an extraordinary objective indicator for early diagnosis of dementia.EEG can provide evidence of impaired brain function for individuals with dementia.