Objective To investigate clinical effects of laparoscopic salpingotomy combined with pituitrin in the treatment of tubal pregnancy.Methods 160 children with tubal pregnancy were chosen in our hospital in the period and randomly divided into two groups including control group (80 children)with laparoscopic salpingotomy treatment and treatment group (80 children)with pituitrin adjunct treatment before operation on the basis of control group.And the operation time,intraoperative bleeding volume,postoperative body temperature,hospitalization time,the retention rate of fallopian tube,fallopian tube patency rate,persistent ectopic pregnancy rate,intrauterine pregnancy rate and fertility rate of both groups were compared.Results The operation time,intraoperative bleeding volume, postoperative body temperature and hospitalization time of the control group were separately (20.33 ±2.70)min, (52.76 ±7.42)mL,(37.52 ±0.54)℃ and (7.56 ±1.98)d;the operation time,intraoperative bleeding volume, postoperative body temperature and hospitalization time of the treatment group were separately (8.94 ±1.61)min, (16.33 ±2.15)mL,(37.46 ±0.50)℃and (5.91 ±1.42)d;the operation time,intraoperative bleeding volume and hospitalization time of the treatment group were significantly better than those of the control group (t=2.26,2.60, 2.08,all P<0.05 );there was no significant difference in postoperative body temperature between 2 groups (t=1.55,P>0.05).The retention rate of fallopian tube and fallopian tube patency rate of the control group were sepa-rately 75.00% (60/80)and 52.50%(42/80);the retention rate of fallopian tube and fallopian tube patency rate of the control group were separately 93.75% (75/80)and 81.25% (65/80);the retention rate of fallopian tube and fallopian tube patency rate of treatment group were significantly better than those of the control group (χ2 =9.71, 10.14,all P<0.05).The persistent ectopic pregnancy rate,intrauterine pregnancy rate and fertility rate of the control group were separately 17.50%(14/80),31.25%(25/80)and 37.50%(30/80);the persistent ectopic pregnancy rate,intrauterine pregnancy rate and fertility rate of the treatment group were separately 15.00% (12/80),61.25%(49/80)and 16.25% (13/80);there was no significant difference in persistent ectopic pregnancy rate between 2 groups (χ2 =1.33,P>0.05);the intrauterine pregnancy rate and fertility rate of treatment group were significant-ly better than those of the control group (χ2 =11.84,12.52,all P<0.05).Conclusion Laparoscopic salpingotomy combined with pituitrin in the treatment of tubal pregnancy can efficiently decrease operation time the amount of bleeding,shorten hospitalization time and be helpful to improve the reproductive outcome.

0.05),the content ratio of glutamate to gamma-aminobutyric acid in the hippocampus of the dependent rats was lower than that in the control group(P

0.05).IGFR-1? expressed in all the 6 cell lines,with the highest expression in SW480(P0.05),but a gefitinib-induced and dose-dependent increase in p-IGFR-1?,not IGFR-1?,expression was observed in HT29 cells(P0.05),but the expression of IGFR-1? was higher in HCT116 than in Lovo and HT29(P

Objective To observe the inhibitory effect of Gefitinib,a selective oral epidermal growth factor receptor tyrosine kinase inhibitor,on the growth of human colon cancer cells,and investigate the relationship between the sensitivity of colon cancer cells to Gefitinib and PIEN expressions.Methods The inhibitory effects of Gefitinib on 6 kinds of colon cancer cells(Lovo,HCT116,HT29,Lsl74T,SW480 and SW620),the levels of PTEN mRNA in different colon cancer cells.and PTEN protein expressions in colon cancer cells were detected by MTT assay,RT-PCR and Westem blot,respectively.Results The inhibitory effects of Gefitinib on the 6 colon cancer cells in vitro varied a lot.When the concentration of Gefitinib was 1 μmol/L,LoVO cells had the most sensitivity to Gefitinib with an IC50＜10μmol/L;HT29 and SW480 had moderate sensitivity,and the IC50 ranged from 10 μnol/L to 100 μmol/L;HCT116,LS174T and SW620 were insensitive to Gefitinib,and their IC50＞100 μmol/L.All the colon cancer cell lines exhibited PTEN mRNA and protein expressions.Conclusions PTEN mRNA andprotein expressions might not be associated with the inhibitory effects of Gefitinib on the growth of colon cancer cells.The expression of PTEN can not be taken as the indication of the sensitivity of colon cancer cells to Gefitinib.

Objective: To use vertical and humidity correcting method to calibrate aerosol optical depth (AOD), and to explore the feasibility of calibrated-AOD as exposure index to measure the level of air pollutants from the ground and to establish the exposure-response relationship between calibrated-AOD and people's health outcomes.Methods: First of all, we interpolated AOD, PM2.5, relative humidity and planetary boundary layer height using Kriging method to obtain data at different locations and matched different data with respiratory death in Beijing by geographical coordinates.Then, the planetary boundary layer height and aerosol hygroscopic growth factor calculated based on relative humidity was used to calbrate the AOD.To compare the effects of calibrated-AOD and PM2.5, we used standardization method to get non dimensionless calibrated-AOD and PM2.5.At last, we used the generalized additive mixed model (GAMM) to estimate the acute effects of calibrated-AOD and PM2.5 on respiratory death and chronic obstructive pulmonary disease (COPD) death, after controlling the time trend, temperature and humidity effects, days of the week effect and holiday effects.Results: The correlation coefficient between calibrated-AOD and PM2.5 was 0.72.The effects of calibrated-AOD on respiratory death and COPD death was the strongest at lag 0-3, one unit of calibrated-AOD increases was associated with 3.64% (95%CI: 0.58%-6.78%) increase of respiratory death and 4.92% (95%CI: 1.81%-8.14%) increase of COPD death.As for PM2.5, the strongest effects appeared at lag 0-1, one unit of PM2.5 (about 155 μg/m3) increases was associated with excess risks of 3.96% (95%CI: 0.82%-7.19%) and 6.12% (95%CI: 1.44%-11.02%) for respiratory death and COPD death respectively.Compared with PM2.5, the effects of calibrated-AOD on respiratory death and COPD death had narrower confidence intervals.The calibrated-AOD was sensitive to capture the lag effects, and the cumulative lag effects of calibrated-AOD were all significant on multiple lag days which indicated that the calibrated-AOD was sensitive to capture cumulative lag effects of air pollutants on respiratory death and COPD death as well.Conclusion: We believe that calibrated-AOD can be used as an index to reflect the effects of air pollutants on respiratory death in Beijing.In the absence of ground monitoring, calibrated-AOD can be used to mea-sure the relationship between air pollutants and some health outcomes.

Objective To explore the effect of modified Seldinger technology in PICC intubation for chemotherapy . Methods Forty patients with chemotherapy during January to December 2014 were assigned as the control group, where the traditional PICC intubation was used, and another forty patients during January to December 2014 as the observation group, where modified Seldinger technology was used. The two groups were compared in terms of post-intubation comfort, puncture success rate, bleeding rate and complications. Results The post-intubation comfort degree of the observational group was significantly higher than that of the control group (P<0.05). The total puncture success rate and one-time success rate were both statistically significantly higher than those of the control group, and the rates of obstruction and infection were significantly lower than those of the control one (P<0.05). Conclusion Modified Seldinger technology used for intubation for chemotherapy can help improve comfort degree and safety and reduce the rate of complications.

Objective To investigate the therapeutic effect of stage treatment of severe complicated injuries in palmar forearm and wrist.Methods From March 2008 to Novemler 2011, eleven cases with skin lost combined with fracture and lost of flexor tendon, ulnar radial arteries, median and ulnar nerves,and some of them had fracture of ulnar and radius were treated in the first stage with vacuum sealing drainage after emergency debridement and tissue repair. Secondary microsurgical repair was performed after the VSD was removed. The lost of median and ulnar nerves was repaired with transplantation of sural nerve,and the wound was repaired with free flaps and complex tissue flaps. Results Eleven cases were treated with vacuum sealing drainage for 7 to 10 days.Infections were under control,wound areas reduced and most wound areas were covered by granulation tissues.Flaps and skin grafts were survived.After postoperative follow-up for 3 to 36 months (average 13 months),three cases was received flexor tendolysis.As result,the blood circulation of hand was good, the sensor of injured nerve was higher than S3, and there was a good functional rehabilitation of hand muscles. Conclusion Using vacuum sealing drainage after emergency debridement and tissue repair, there was a good repairing effect of secondary repair with flaps and skin grafts for severe complicated tissue defect in palmar forearm and wrist,and it is an effective choice of operation.

Objective To determine the evaluating prognostic value of plasma Nt-proBNP in patients with congestive heart failure.Methods 107 patients with congestive heart failure were included in this study,followed the degree of rehospitalization and mortality rate of the cured patients after left hospital.The plasma concentrations of Nt-proBNP were measured by enzymelinked immunozorbent assay(ELISA).Results Concentrations of Nt-proB-NP of Ⅱ,Ⅲ,Ⅳ classincation were(1129.8±383.2),(5849.7±567.6),(9506.3±492.4)ng/L in NYHA functional classification respectively(P＜0.01);the plasma level of Nt-proBNP had significant positive correlation with NYHA functional classification(r=0.759,P＜0.01).The degree of rehospitalization were(0.85±0.96),(1.98±0.86),(2.58±0.91)respectively(P＜0.01);mortality rate were 8.3%,12.5%,45.2%respectively(P＜0.01).Conclusion Plasma Nt-proBNP level may be the more important clinic value in evaluating prognosis in patients with congestive heart failure.

Objective To evaluate the dose distributions with different dose rates, and approach a reference to the dose rate for radiation. Methods Three classic static intensity modulated radiotherapy (IMRT) plans of prostate cancer, lymphoma and nasopharyngeal carcinoma were chosen for the study. For each plan, the dose verification of three different dose rates (100, 300 and 600 MU/min) was performed with the Varian 600CD linear accelarator by using the 2-DICA of I'mRT Matrixx. With the Pinncale planning system, each segment was used as a beam to form another IMRT plan. The OmniPro-I'mRT V1.6 was applied to compare the segments in the two IMRT plans, and then the actual weights were obtained. The simulated plans at different dose rate were designed when setting the weights back into the planning system. Results With the increase of dose rate, the passing ratio of the verification decreased and the D_(max), D_(min), D_(mean) and D_(95) of the planning tumor volume increased. The high dose area expanded significantly in target regions, and the 95% isodose line extended. At the dose rate of 600 MU/min, The D_(95) of GTV_(nd) in nasopharyngeal carcinoma increased by 5.33% than the original plan with the V_(110) up to 19. 38%. The irradiation dose of the organs at risk (OARs) increased. For the case of lymphoma, the V_(20) of the lungs in the original plan and the three simulated plans were 31.77%, 32. 11%, 32.60% and 33.26%, respectively. For the case of nasopharyngeal carcinoma, the V_(30) of the right parotid were 48.75%, 49. 56%, 51.65% and 53.91%, respectively. Conclusions With the increase of dose rate in static IMRT , the actual dose distribution deviates the original plan , and the high dose area and the OARs dose increases. The higher dose rate is suboptimal when the dose of the OARs is proximate to the tolerance limit.

Objective To explore the value of dual-source CT monoenergetic-plus imaging in the diagnosis of early gastric cancer. Methods Imaging data of 2 6 cases with pathologically proven early gastric cancer who underwent dual-energy scanning were analyzed retrospectively.Monoenergetic-plus images with energy levels at 40 keV,50 keV,60 keV and 70 keV were reconstructed by using an advanced monoenergetic software (syngo.via.VA30A,Dual energy Mono+).Contrast-to-noise ratio (CNR)of the lesion,image noise,overall image quality score and lesion conspicuity score were com pared between different monoenergetic-plus images and conventional polychromatic images at 1 2 0 kVp.Results The CNRs (3 .2 4 ± 0 .7 6 ,2 .9 4 ± 0 .9 2 ,2 .4 7 ± 0 .7 9 ,2 .3 7 ± 0.98 and 1.22±0.40 respectively)and image noises [(32.7±1.82)HU,(25.6±2.61)HU,(20.30±2.1 7)HU,(1 7.03±2.1 5)HU and (18.15±2.12)HU respectively]among 40 keV,50 keV,60 keV,70 keV and 120 kVp groups all had statistical differences (F =24.654 and 227.05,P <0.01).The CNR in 50 keV group was higher than that in 60 keV,70 keV and 120 kVp groups,but similar to that in 40 keV group (P >0.05).The SD value in 50 keV group was lower than that in 40 keV group,but higher than that in other three groups (P <0.05).The overall image quality score (3.08±0.27,3.65±0.49,3.31±0.47,3.23±0.43 and 3.1 5±0.37,respectively) and lesion conspicuity score (4.15±0.67,3.92±0.48,3.27 ±0.45,3.08±0.27 and 3.04±0.20)among the five groups all had statistical differences (Z =25.343 and 70.426,P <0.05).The overall image quality score of 50 keV group was higher than that of the other four groups (P <0.05).Lesion conspicuity score in 50 keV group was higher than that in 60 keV,70 keV and 120 kVp groups and similar to that in 40 keV group (P =0.1 52).Conclusion The 50 keV monoenergetic-plus images can increase the CNR value of early gastric cancer while maintaining the overall image quality.