OBJECTIVE: To investigate the expression of FasL and ICAM-1 on the HEVs in the hypertrophic tonsils and its clinical significance. METHOD: Under the light microscopy, HE and immunohistochemistry methods were used to show the morphology of HEVs, the number of T, B lymphocytes, the expression intensity of FasL and ICAM-1 in 17 patients with the idiopathic tonsillar hypertrophy (ITH) and in 20 patients with the chronic hypertrophic tonsillitis (CHT). RESULT: The expression intensity of ICAM-1 on the tonsillar HEVs in ITH group was significantly lower than that in CTH group (P < 0.05), but the intensity of FasL in ITH group was significantly higher than that in CHT group (P < 0.05). CONCLUSION FasL and ICAM-1 expression in the tonsillar HEVs contribute to the CHT and ITH. The former mainly makes IHT developed, and the latter promotes the process of the inflammation.
Adolescent ; B-Lymphocytes ; immunology ; Child ; Child, Preschool ; Endothelium ; blood supply ; Fas Ligand Protein ; metabolism ; Female ; Humans ; Hypertrophy ; Intercellular Adhesion Molecule-1 ; metabolism ; Male ; Palatine Tonsil ; metabolism ; pathology ; T-Lymphocytes ; immunology ; Tonsillitis ; metabolism ; Venules ; metabolism

OBJECTIVETo study the significance and differential diagnosis of intralymphatic accumulation of lymphocytes.

METHODSThe clinical and pathologic features of 4 cases of intralymphatic accumulation of lymphocytes were reviewed retrospectively. Immunohistochemical study was carried out and follow-up data were analyzed.

RESULTSThe sites of involvement included tonsil (2 cases), pharynx (1 case) and appendix (1 case). The duration of disease ranged from 1 week to 3 months. Follow up of the patients (from 3 to 84 months) showed no evidence of disease recurrence. Gross examination of the tissues (except in the case of appendiceal involvement) showed polypoid changes. Histologically, the lymphatic channels were filled up with small lymphocytes and associated with fibrosis in the vicinity. Immunohistochemical study revealed a T-cell phenotype of the intralymphatic lymphoid cells.

CONCLUSIONSThe accumulation of lymphocytes in lymphatic channels is associated with a benign clinical course. This phenomenon may be due to retention of lymphocytes secondary to the perilymphatic chronic inflammation and fibrosis. Although the lesion simulates intravascular lymphomatosis morphologically and shows a uniform T-cell phenotype, the lymphoid cells lack obvious cellular pleomorphism and mitotic activity. The solitary nature of the lesion, when coupled with the indolent clinical behavior, is also helpful in the differential diagnosis.

Adolescent ; Adult ; Antibodies, Monoclonal, Murine-Derived ; metabolism ; CD3 Complex ; metabolism ; Child ; Diagnosis, Differential ; Female ; Fibrosis ; Follow-Up Studies ; Humans ; Lymphangitis ; metabolism ; pathology ; Lymphatic Diseases ; metabolism ; pathology ; Lymphatic Vessels ; pathology ; Lymphoma, B-Cell ; metabolism ; pathology ; Male ; Middle Aged ; Palatine Tonsil ; pathology ; Platelet Endothelial Cell Adhesion Molecule-1 ; metabolism ; Retrospective Studies ; T-Lymphocytes ; pathology ; Young Adult

To investigate the role of cyclin B1 and cdc2 in the pathogenesis and progression of malignant lymphoma, 68 cases of nodal non-Hodgkin's lymphoma were examined about the expression of cyclin B1 and cdc2 along with p53 and Ki-67 by immunohistochemical method. The correlation of their expression with various clinicopathologic findings was also analyzed. Cyclin B1 and cdc2 were diffusely expressed in 39 cases (57.4%) and 54 cases (79.4%) out of 68 cases studied, respectively. The mean labeling indices of cyclin B1 and cdc2 in malignant lymphoma were 31.9% and 68.0%, respectively. In normal lymphoid tissues, cyclin B1 and cdc2 were expressed predominantly in the germinal center with mean labeling indices of 13.9% and 28.3%, respectively. The correlation between the expression of cyclin B1 and cdc2 was noted (p=0.013). The expression of Ki-67 was correlated with that of cyclin B1 (p=0.023) and marginally correlated with that of cdc2 (p=0.056). The expression of cdc2 and p53 in complete remission group to chemotherapy was lower than that of progressive disease group (p=0.047, p=0.049). In multivariate analysis, the clinical stage alone showed significance on overall survival (p=0.049). In conclusion, cyclin B1 and cdc2 appeared to be involved in the genesis or progression of malignant lymphoma and cdc2 can be a useful marker for response to chemotherapy.
Adolescent ; Adult ; Aged ; CDC2 Protein Kinase/*biosynthesis ; Child ; Child, Preschool ; Cyclin B/*biosynthesis ; Cyclin B1 ; Female ; Humans ; Immunohistochemistry ; Ki-67 Antigen/biosynthesis ; Lymph Nodes/metabolism/pathology ; *Lymphoma, Non-Hodgkin/metabolism/mortality/pathology ; Male ; Middle Aged ; Palatine Tonsil/metabolism/pathology ; Predictive Value of Tests ; Prognosis ; Survival Analysis ; Tumor Suppressor Protein p53/biosynthesis