Objective To probe into the way for relieving pains irrelevant to incision but associated with the event of pneumoperitoneum by means of physiotherapy, wherein the said pains occur after a gynecologic celioseope operation.Methods Random option of 207 patients after the gynecologic operation was conducted and the patients were divided into 3 groups at random that were on the man-care,instrument and ordinary basis.Each group consists of 69 cases.In man-care group,artificial massage at the points (hereafter called mann-care group)Was adopted.The instrument group adopted microwave devices to heal (hereafter called instrument group).And the ordinary group adopted regular care of post operation (hereafter called ordinary group).We observed and compared three methods,to see whether they may relieve the pains irrelevant to incision.Results The point massage and microwave therapy can relieve the complicated pains irrelevant to incision after a gynecologic operation,and the effect thereof is obviously better than that of the ordinary care.Conclusions As to the complications such as pains irrelevant to incision after gynecologic operation,the point massage,microwave and relevant physiotherapy can function well and are comparatively ideal.

To evaluate the inhibitory effects of long antisense RNA on HBV replication in HepG2.2.15 cells. The coding region of HBV S gene was cloned into pTARGET vector in sense and antisense orientations and the recombinant plasmids were transfected into HepG2.2.15 cells which were divided into HBS2 (antisense RNA) group, HBS4 (sense RNA) group and control group. HBsAg and HBeAg in the culture supernate were detected by ELISA. The HBV DNA in the supernate was quantified by real-time PCR. After treatment, the levels of HBsAg in HepG2.2.15 cell supernatants of three groups were 0.621+/-0.027, 3.399+/-0.018 and 2.232+/-0.187 respectively; the levels of HBeAg were 0.749+/-0.019, 1.548+/-0.025 and 1.570+/-0.044 respectively and the levels of HBV DNA were 1.597+/-0.082, 3.381+/-0.297 and 3.610+/-0.063 respectively. The expressions of HBsAg and HBeAg and the HBV DNA level in HBS2 group were remarkably reduced as compared to the control (Z = -2.309, P value is less than 0.05); whereas the sense plasmid transfection (HBS4) did not affect HBeAg (Z = -0.866) and HBV DNA (Z = -1.155) levels in the culture supernate but slightly increased the HBsAg level (Z = -2.309). Antisense RNA might be a useful tool to repress HBV replication.
DNA, Viral ; genetics ; Gene Expression Regulation, Viral ; Genetic Vectors ; Hep G2 Cells ; Hepatitis B Surface Antigens ; analysis ; Hepatitis B e Antigens ; analysis ; Hepatitis B virus ; genetics ; physiology ; Humans ; Plasmids ; RNA Interference ; Transfection ; Virus Replication ; genetics