As natural components of the environment,air and water,geomagnetic field plays an important role,which is closely related to growth and evolution for living organisms. Being off earth magnetic field,biological effects would be fetched out. Settled in the Moon and long-term interplanetary space flight,human being and other biological objects would experience extremely low magnetic fields. In addition,weaker magnetic fields exist in some artificial environment,such as artificial magnetic shielding experimental room,submarine etc. Since 1970s,biological effects of hypomagnetic environment and zero-magnetic environment have continuously studied. This paper will briefly review the research progress about biological effects of hypomagnetic environment..

OBJECTIVE:To establish a method for the determination of EPA and DHA in ray oil.METHODS:The determination was carried out on Diamonsil C18 column.The mobile phase consisted of acetonitrile-water(95 :5) with flow rate of 1.0 mL?min-1 and detection wavelength set at 220 nm.The column temperature was set at 25 ℃.RESULTS:The linear ranges were 1～4 ?g for EPA(r=0.999 1) and 2～8 ?g for DHA(r=0.999 4).The average recoveries were 99.23% for EPA(RSD=0.49%) and 99.30% for DHA(RSD=0.27%).CONCLUSION:The method is simple,rapid,accurate and reproducible for the detection of EPA and DHA in ray oil.

Degree of Confidence(?),Validity(E),Item Difficulty(P) and Item Discrimination(D) used in Software System for Paper Quality Analysis play an important role in evaluating the qualities of items and papers.Their usages and meanings are discussed in detail here,and an example is given to illustrate their significance and important roles in items analysis and papers analysis.

Paper analysis plays an important role in teaching improvement as well as in its quality enhancement.We have developed a set of software for analyzing paper qualities,which will make paper analysis efficient,practical and standardizing.

Objective To evaluate the value of color Doppler flow imaging(CDFI) in diagnosis of choroidal melanoma.Methods A comparative analysis was carried retrospectively on sonograms,pathological and clinical data of 13 choroidal melanoma cases,confirmed by pathology and operation.Results The pre-operative diagnosing rate of choroidal malignant melanoma by CDFI in the study was 100%.The sonogram features were divided into three types: hemispherical type,mushroom type and diffusive type.Conclusions Characters of blood flow signal of intraocular occupying lesions were valuable to distinguish choroidal malignant melanoma from other diseases.

BACKGROUND: Long-term utilization of steroid hormone easily induces avascular necrosis of the femoral head (ANFH). Cornu Cervi Pantotrichum possesses bone growth factor and can promote wound healing and tissue repairing, which may has effect on treatment of ANFH. OBJECTIVE: To investigate therapeutic effect of antler powder on ANFH induced by corticosteroids in rats. METHODS: Totally 42 male wistar rats were randomly divided into the blank control, model and high-, moderate- and low-dose treatment groups. Exception those in the blank control group, all rats were intragluteally injected with dexamethasone at 30 mg/kg twice per week for 6 weeks to induce ANFH. Then the rats were treated with antler powder by oral gavage at 200, 400 and 800 mg/kg dosage, once per day for 60 days. Same volume of physiological saline was gavaged in the blank control and model groups. Serum lipid level and bone mineral density (BMD) of femur were measured, pathological change and expression of vascular endothelial growth factor (VEGF) were investigated. RESULTS AND CONCLUSION: Steroid hormone intervention resulted in obviously ANFH in rats. After treatment with antler powder, the degree of necrosis was significant reduced. Compared with the model group, BMD were increased, total cholesterol, triacylglycerol, and low density lipoprotein levels were decreased, and the VEGF expression increased in the treatment groups. The results suggest that antler powder has a positive curative effect on ANFH by promoting bone formation, fat metabolism and increasing VEGF expression.

Objective:This work aims to use the characteristics of dendritic cells (DCs) pulsed with recombinant human HSP70, which can present and process tumor antigens, to enhance the killing activity of cytotoxic t lymphocytes (CTLs) against breast neoplasms. Methods:Autologous DCs were isolated from peripheral blood mononuclear cells and then stimulated in vitro with granulocyte macrophage-colony stimulating factor and interleukin-4. The DCs were loaded with A549 tumor cell freeze-thaw lysate, and rhHSP70 was added as an immune adjuvant. The specific groups were subjected to tumor-specific cytotoxic assay, enzyme-linked immunosorbent assay, and fluores-cence-activated cell sorting. Results:DCs pulsed with A549 tumor cell lysate enhanced the growth expansion of CTLs, upregulated CD40 and CD80 populations in CTLs, and augmented Th1 cytokines. In addition, the cytotoxicity of specific CTLs against A549 was highly enhanced. The above indications became more obvious after the addition of rhHSP70. Conclusion:DCs pulsed with freeze-thaw cell lysates derived from breast cancer can enhance growth expansion of lymphocytes. They may serve as an effective tumor antigen to stimulate the proliferation of specific CTLs, which are very effective in activating specific T-cell responses against breast cancer cells in vitro. The improved anti-tumor immunity response by DC-based vaccines may be related to the maturation of the DCs promoted by rhHSP70.

Objective To make use of the characteristics of presenting and processing tumor antigen of Dendritic cells pulsed with recombinant human Hsp70 and Mage-3 peptide to enhance killing capability of breast neoplasms. Methods Autologous dendritic cells were isolated from PBMC and then stimuiated in vitro with GM-CSF and IL-4.Dendritic cells were loaded with Mage-3 peptide;at the same time Hsp70L1 was added. The specific groupings each induce generation of tumor specific cytotoxic assay with ELISA. Results DCs pulsed with Mage-3 peptide enhanced the growth expansion of CTL, and promoted the secretion of cytokines such as TNF-αand IL-6. DCs pulsed with recombinant human Hsp70 and Mage-3 peptide had cytotoxicity against human breast cancer cells MCF-7.Conclusions DCs pulsed with recombinant human Hsp70 and Mage -3 peptide have higher biological activities. Modified DCs can stimulate the proliferation of lymphoeytes,present effectively tumor antigen to stimulate generation of speeific CTLs. Dendritic cells pulsed with recombinant human Hsp70 and Mage-3 peptide have high ability to kill breast cancer cells.

Objective To study the feasibility and safety of the diagnostic coronary angiography by transradial approach with modified 5 F universial catheter.Methods Retrospective analysis was performed in 268 randomly chosen patients undergoing diagnostic coronary angiography by transradial approach,either with 5 F universial catheter(universial group,n=133) or 5 F modified universial catheter(modified group,n=135).The success rate and the duration of operation and fluoroscopy,the rate of error entry into branch vessel between the 2 groups were compared.Results The success rate for angiography was 87.2%(116/133) in the universal group and 97.0%(131/135) in the improved group(P

Objective To observe the effect of attention after sleep deprivation in healthy volunteers.Methods10 healthy young male volunteers were exposed to continuous sleep deprivation for 32 h.Continuous performance task(CPT) and Stroop tests were performed 4 times to the volunteers at 17 h and 32 h before and after sleep deprivation.Results In CPT and Stroop test,the total reaction time and error rate were elevated significantly after sleep deprivation(all P